LOCUS       RR217225                7314 bp    DNA     circular  SYN 
DEFINITION  Tlr10 (myc-DDK-tagged) - Rat toll-like receptor 10 (Tlr10)
ACCESSION   NM_001146035
VERSION     NM_001146035.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3471..3562 
                     /label="Myc-DDK"  
     misc_feature    3592..4177 
                     /label="PolyA signal"  
     misc_feature    3649..3668 
                     /label="Restriction Enzyme"  
     misc_feature    4453..5125 
                     /label="ColE1"  
     misc_feature    5555..6349 
                     /label="Kan/Neo"  
     misc_feature    6384..6686 
                     /label="SV40 ori"  
     misc_feature    6772..7249 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3453..3459 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3453 
                     /label="Tlr10"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAGACTCATC AGCAGCATCT ATACCCTTTA TAACATCATT ATGTCAACAG 
     1081 AAGCCTGGGC TCCAAGACCC ACAGATGAAA GGGAACTGAT CTCCAACTAC TCCAACATGG 
     1141 CTTTAAGGAA GGTTCCCCCA GATGTGACCC CAGCCACAAC CATACTGGAT TTATCCTACA 
     1201 ACCTCCTCTA TCAATTCCAG AGCTCAGATT TCCACTCCTT CTCCAAACTG CGTTTTCTGA 
     1261 TCCTGTGCCA TAACAGCATC CAACAGCTGG ACACCAAGAT CTTTGACCTT AACAAGGAGC 
     1321 TAAGCTATTT AGACTTGTCT TACAATAGAC TACAGTCCAT CACTTGGTCT TCCTTGGTAG 
     1381 ATCTCAGACA TTTAGATCTT TCTTTTAACG ACTTCGATAC TCTGCCTGCC TGTGAGGAGA 
     1441 TTGGCAACAT GCGACATCTG GAAACCTTAG GTTTGAGTGG GGCCAAAATC CAAAGATCAG 
     1501 ATCTCCAGAA AATTGCTAAT TTGCAATTCA GCACTGTGTT CATAGGGCTG AATACTCTTT 
     1561 CTCACTATGA AGAAGGCAGC CTGCCTGTCC TAAACACGAC AGTGCTTCAC ATAGTTTTAC 
     1621 CAATGGCTGC TAACTTCTGG GTGCTTCTGT GTGATGGAAT GAAGACTTCA AAGGTAGTCG 
     1681 AAATGTCAAA CATAGGTGGC AAGAGCCAGT TTGTAAGTTA TGAGACTCCG AGAACTCTTT 
     1741 CTGTGGAGAA TACCAAGGCG TCCACTCTTA TATTCCATAA AGTTGACCTG CTCTGGGATG 
     1801 ACCTTCTCCA CGTTTTTCAG TTTGTGTGGC AGACTTCAGT GGAATGCTTC CAGATTCAGG 
     1861 ACCTGACTTT TGGAGGGATG GCTAGTCGGG ACCACAGTTC ATTTGACTAC TCAAATACCG 
     1921 TAATGAAAGC TGTAATCTTG GAGAGCATAC ATTTCAGAAT CTTTTTCATT CCCCAGAATA 
     1981 AAATCTACTT GTTTTTCACC CAAATGGATT TAGAAAACCT GACGATATCA GATGCACAAA 
     2041 TGCCACATCT TCTCTTCCCT AGTCACCCCC AAAGTTTCCA ACATTTAAAT TTTGCCAATA 
     2101 ACATCTTAAC AGATGACCTG TTTAAAAACC CCATCCAGAT GCCTTATCTG AAAACTCTCG 
     2161 TTTTGAGGGG CAACCAACTG GAGACTCTTT CTCTAGTGAG TCTCTTTGCC AACCACACGT 
     2221 CCTTGCTTCA CCTGGATCTG AGCCAGAACC TATTGCAACA TGGAAACGGG GAGAAATGCA 
     2281 TGTGGCCCGA CACCTTGATC CTCTTGAACT TGTCATCCAA TAAATTTGAT GGTTCTGTTT 
     2341 TCAGATGTTT GCCTAAAAGT ATCCAGACAC TTGATCTGAA CAATAACAAA ATTCGAGCAG 
     2401 TCCCTAAAGA GATTATATAC CTAAAATCTT TACGAGAGCT AAGTATTGCT TTTAATTTTC 
     2461 TGACTGACCT CCCTGGGTGT GATCACTTCA AACGGCTCTC TAGTCTGAAC ATTGAAATGA 
     2521 ATTTGATTCT CAGCCCATCT CTGGATTTTT TCCAGAGCTG CCAGGAGGTA AAGACTCTAA 
     2581 ACGCAGGGCG AAATCCATTC CGGTGTATCT GTGAATTTAG AGACTTCATT TGGCTTGAGA 
     2641 AACATTCCAA GGGCCTGATG TCTGAATGGC CAGACTCCTA CGCCTGCGAA TACCCTTTGA 
     2701 GTCTAAAGGG GACTCCATTA AAAGATGTTT ATCTTCCCGA ATTAGCTTGC AACACAACTT 
     2761 TGCTGATTGT CACCATTGTG CTGGTTATCC TGGTTCTGGG TTTGGTTGTA GCATTCTGCA 
     2821 TTCTAAAATT TGACATGCTT TGGTATCTCA GGGTGCTGGC CCAGTGCATC CGGACACAGC 
     2881 ATAGGGTTAG GAAGACAGTT CGAGAGGAGC GTAAGAGAAG TGTACAGTTC CATGCATTTA 
     2941 TCTCCTACGC TGCACATGAT TCTGTCTGGG TGAAGAATGA ACTGATTCCC AATCTGGAGA 
     3001 CAGAAGATGG CTCTGTCTTG CTTTGTCTTC CCGAGAGGAA CTTTGACCCT GACAGGAGCA 
     3061 TTGCTGAAAA TATTATAAGC TGCACTGAGA AAAGCTATAA GTCCATCTTT GTGCTGTCCC 
     3121 CCAACTTTGT GCAGAATGGG TGGTGCCATA ATGAACTCTA TCTGGCCCAC CACAATCCTT 
     3181 TCCGTGACAA TTATGATTAC ATCATTCCCA TCTTACTAGA ACCCATTCCC TCCTACTGTG 
     3241 TTCCTACCAA GTTCCCTAAG CTGAAAGCTC TTTTGGAAAA GAAAGTATAC TTAGAGTGGC 
     3301 CCAAGGACAG GCGCAAACAT GGGCTTTTTT GGGCAAACCT TCGAGCTGCC ATTAACGTAA 
     3361 ATTTATCAGA CACAAGGGGA AGGTGTGAAC TACAGACATT CACAAAGCCT AACGCGGATT 
     3421 GCCAAGGTTC TACCATCTCT CTGGTAAGAA CAGACAGCTT CACGCGTACG CGGCCGCTCG 
     3481 AGCAGAAACT CATCTCAGAA GAGGATCTGG CAGCAAATGA TATCCTGGAT TACAAGGATG 
     3541 ACGACGATAA GGTTTAAACG GCCGGCCGCG GTCATAGCTG TTTCCTGAAC AGATCCCGGG 
     3601 TGGCATCCCT GTGACCCCTC CCCAGTGCCT CTCCTGGCCC TGGAAGTTGC CACTCCAGTG 
     3661 CCCACCAGCC TTGTCCTAAT AAAATTAAGT TGCATCATTT TGTCTGACTA GGTGTCCTTC 
     3721 TATAATATTA TGGGGTGGAG GGGGGTGGTA TGGAGCAAGG GGCAAGTTGG GAAGACAACC 
     3781 TGTAGGGCCT GCGGGGTCTA TTGGGAACCA AGCTGGAGTG CAGTGGCACA ATCTTGGCTC 
     3841 ACTGCAATCT CCGCCTCCTG GGTTCAAGCG ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG 
     3901 GGATTCCAGG CATGCATGAC CAGGCTCAGC TAATTTTTGT TTTTTTGGTA GAGACGGGGT 
     3961 TTCACCATAT TGGCCAGGCT GGTCTCCAAC TCCTAATCTC AGGTGATCTA CCCACCTTGG 
     4021 CCTCCCAAAT TGCTGGGATT ACAGGCGTGA ACCACTGCTC CCTTCCCTGT CCTTCTGATT 
     4081 TTAAAATAAC TATACCAGCA GGAGGACGTC CAGACACAGC ATAGGCTACC TGGCCATGCC 
     4141 CAACCGGTGG GACATTTGAG TTGCTTGCTT GGCACTGTCC TCTCATGCGT TGGGTCCACT 
     4201 CAGTAGATGC CTGTTGAATT GGGTACGCGG CCAGCGGCGA GCGGTATCAG CTCACTCAAA 
     4261 GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA 
     4321 AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT 
     4381 CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC 
     4441 AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC 
     4501 GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC 
     4561 TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG 
     4621 TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA 
     4681 GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG 
     4741 CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA 
     4801 CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG 
     4861 AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG 
     4921 CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC 
     4981 GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC 
     5041 AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG 
     5101 TATATATGAG TAACCTGAGG CTATGGCAGG GCCTGCCGCC CCGACGTTGG CTGCGAGCCC 
     5161 TGGGCCTTCA CCCGAACTTG GGGGGTGGGG TGGGGAAAAG GAAGAAACGC GGGCGTATTG 
     5221 GCCCCAATGG GGTCTCGGTG GGGTATCGAC AGAGTGCCAG CCCTGGGACC GAACCCCGCG 
     5281 TTTATGAACA AACGACCCAA CACCGTGCGT TTTATTCTGT CTTTTTATTG CCGTCATAGC 
     5341 GCGGGTTCCT TCCGGTATTG TCTCCTTCCG TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC 
     5401 GAAGAACTCC AGCATGAGAT CCCCGCGCTG GAGGATCATC CAGCCGGCGT CCCGGAAAAC 
     5461 GATTCCGAAG CCCAACCTTT CATAGAAGGC GGCGGTGGAA TCGAAATCTC GTGATGGCAG 
     5521 GTTGGGCGTC GCTTGGTCGG TCATTTCGAA CCCCAGAGTC CCGCTCAGAA GAACTCGTCA 
     5581 AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA TCGGGAGCGG CGATACCGTA AAGCACGAGG 
     5641 AAGCGGTCAG CCCATTCGCC GCCAAGCTCT TCAGCAATAT CACGGGTAGC CAACGCTATG 
     5701 TCCTGATAGC GATCCGCCAC ACCCAGCCGG CCACAGTCGA TGAATCCAGA AAAGCGGCCA 
     5761 TTTTCCACCA TGATATTCGG CAAGCAGGCA TCGCCATGGG TCACGACGAG ATCCTCGCCG 
     5821 TCGGGCATGC TCGCCTTGAG CCTGGCGAAC AGTTCGGCTG GCGCGAGCCC CTGATGCTCT 
     5881 TCGTCCAGAT CATCCTGATC GACAAGACCG GCTTCCATCC GAGTACGTGC TCGCTCGATG 
     5941 CGATGTTTCG CTTGGTGGTC GAATGGGCAG GTAGCCGGAT CAAGCGTATG CAGCCGCCGC 
     6001 ATTGCATCAG CCATGATGGA TACTTTCTCG GCAGGAGCAA GGTGAGATGA CAGGAGATCC 
     6061 TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG TCCCTTCCCG CTTCAGTGAC AACGTCGAGC 
     6121 ACAGCTGCGC AAGGAACGCC CGTCGTGGCC AGCCACGATA GCCGCGCTGC CTCGTCTTGC 
     6181 AGTTCATTCA GGGCACCGGA CAGGTCGGTC TTGACAAAAA GAACCGGGCG CCCCTGCGCT 
     6241 GACAGCCGGA ACACGGCGGC ATCAGAGCAG CCGATTGTCT GTTGTGCCCA GTCATAGCCG 
     6301 AATAGCCTCT CCACCCAAGC GGCCGGAGAA CCTGCGTGCA ATCCATCTTG TTCAATCATG 
     6361 CGAAACGATC CTCATCCTGT CTCTTGATCG ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA 
     6421 GCCTCCTCAC TACTTCTGGA ATAGCTCAGA GGCCGAGGCG GCCTCGGCCT CTGCATAAAT 
     6481 AAAAAAAATT AGTCAGCCAT GGGGCGGAGA ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG 
     6541 GATGGGCGGA GTTAGGGGCG GGACTATGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     6601 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCTG GTTGCTGACT AATTGAGATG 
     6661 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC CTAACTGACA 
     6721 CACATTCCAC AGCTGGTTCT TTCCGCCTCA GGACTCTTCC TTTTTCAATA TTATTGAAGC 
     6781 ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA 
     6841 CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGCGCC CTGTAGCGGC 
     6901 GCATTAAGCG CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC 
     6961 CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC 
     7021 CGTCAAGCTC TAAATCGGGG GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC 
     7081 GACCCCAAAA AACTTGATTA GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG 
     7141 GTTTTTCGCC CTTTGACGTT GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT 
     7201 GGAACAACAC TCAACCCTAT CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT 
     7261 TCGGCCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA TTTT
//