LOCUS       RR217189                7182 bp    DNA     circular  SYN 
DEFINITION  Sec23b (myc-DDK-tagged) - Rat Sec23 homolog B (S. cerevisiae) (Sec23b)
ACCESSION   NM_001277235
VERSION     NM_001277235.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3339..3430 
                     /label="Myc-DDK"  
     misc_feature    3460..4045 
                     /label="PolyA signal"  
     misc_feature    3517..3536 
                     /label="Restriction Enzyme"  
     misc_feature    4321..4993 
                     /label="ColE1"  
     misc_feature    5423..6217 
                     /label="Kan/Neo"  
     misc_feature    6252..6554 
                     /label="SV40 ori"  
     misc_feature    6640..7117 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3321..3327 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3321 
                     /label="Sec23b"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCAACATAT CTGGAATTCA TTCAGCAGAA TGAAGAACGA GATGGTGTGC 
     1081 GTTTCAGTTG GAATGTGTGG CCTTCTAGCC GACTTGAAGC CACAAGAATG GTTGTTCCCT 
     1141 TGGCTTGTCT CCTCACTCCT TTAAAAGAGC GCCCAGACTT ACCTCCTGTA CAGTATGAAC 
     1201 CTGTGCTTTG CAGTAGGCCT ACCTGCAAAG CTATTCTCAA TCCACTTTGT CAAGTTGATT 
     1261 ACCGAGCAAA ACTTTGGGCC TGCAATTTCT GTTTTCAAAG AAATCAGTTC CCCCCAGCAT 
     1321 ATGCAGGCAT TTCTGAGGTG AACCAGCCTG CTGAATTGAT GCCCCAGTTC TCCACAATCG 
     1381 AGTACATGAT CCAGAGAGGT GCTCGGTCCC CTCTGATCTT TCTCTATGTG GTTGACACGT 
     1441 GTTTGGAAGA AGATGACCTT CAAGCACTCA AAGAGTCGCT GCAGATGTCT CTGAGTCTCC 
     1501 TTCCTCCAGA TGCCCTGGTT GGTCTTATCA CTTTTGGGAG GATGGTGCAG GTTCATGAGC 
     1561 TAAGCTGTGA AGGAATCTCC AAGAGCTATG TCTTCCGAGG GACCAAGGAT TTAACTGCAA 
     1621 AGCAAATACA GGAGATGCTG GGCCTGACCA AGTCAGCTAT GCCTGTGCAG CAAGTCAGAC 
     1681 CTGGTCAACC TCAGGAGCAG TCTGCTGTTT CCAGCAGATT TCTACAGCCC GTTCACAAGA 
     1741 TTGACATGAA CCTCACAGAT CTTCTTGGGG AGCTGCAGAG GGACCCATGG CCCGTAACTC 
     1801 AGGGGAAGAG ACCACTGAGA TCCACTGGTG TTGCTTTGTC CATTGCTGTT GGCTTGTTGG 
     1861 AGGGTACATT CCCAAATACT GGAGCCAGGA TCATGCTGTT CACTGGTGGG CCCCCGACCC 
     1921 AAGGACCTGG CATGGTGGTT GGAGATGAAT TGAAGACTCC TATTCGTTCC TGGCACGATA 
     1981 TTGAGAAAGA TAATGCACGG TTCATGAAAA AGGCAACCAA GCATTATGAG ATGCTTGCTA 
     2041 ATCGAACTGC TACAAATGGT CACTGCATTG ATATTTATGC TTGTGCCCTT GATCAAACTG 
     2101 GACTTCTGGA GATGAAGTGC TGTCCAAATC TTACAGGAGG CCTCATGGTG ATGGGAGACT 
     2161 CATTCAACAC CTCTCTCTTC AAGCAGACCT TCCAAAGGAT TTTTAGTAAA GACTTCAACG 
     2221 GAGATTTCAG AATGGCGTTT GGTGCTACCC TGGAAGTCAA GACCTCTCGG GAACTGAAGA 
     2281 TTGCAGGTGC CATTGGTCCA TGTGTGTCTC TGAATGTGAA AGGACCATGT GTGTCAGAAA 
     2341 ATGAGCTCGG CGTTGGTGGC ACAAGTCAGT GGAAGATCTG TGGCCTGGAT CCTTCGTCTA 
     2401 CTCTTGGCAT CTACTTTGAA GTTGTCAATC AGCACAATGC CCCGATTCCC CAGGGAGGCA 
     2461 GAGGTGCCAT CCAGTTTGTC ACTCAGTATC AGCATTCCAG CACTCAGAAG CGCATCCGAG 
     2521 TGACCACCAT TGCCCGCAAT TGGGCAGATG CACAGAGTCA GCTGAGGCAC ATAGAAGCAG 
     2581 CGTTTGACCA GGAGGCTGCA GCAGTCTTGA TGGCACGGCT TGGAGTGTTC CGGGCAGAGT 
     2641 CAGAGGAGGG GCCTGACGTG CTCCGGTGGC TGGATCGACA ACTCATCAGA CTGTGTCAGA 
     2701 AGTTTGGACA GTATAACAAA GAAGACCCCA CGTCTTTCAG GCTGTCAGAC TCCTTTTCTC 
     2761 TGTATCCTCA GTTCATGTTC CATCTGAGGA GATCTCCGTT TCTTCAAGTG TTTAACAACA 
     2821 GTCCTGACGA GTCGTCCTAT TATAGACACC ATTTTGCCCG GCAGGATCTG ACTCAGTCCC 
     2881 TCATCATGAT CCAGCCCATT CTCTACTCTT ACTCCTTCCA CGGACCCCCA GAGCCTGTAC 
     2941 TCCTGGACAG CAGTAGCATT CTAGCTGACA GAATTCTGCT GATGGATACT TTCTTCCAAA 
     3001 TTGTCATTTA CCTTGGTGAG ACCATAGCCC AGTGGCGGAA AGCTGGCTAC CAGGACATGC 
     3061 CTGAGTATGA GAACTTCAAG CACCTTCTGC AGGCACCGCT GGATGATGCC CAAGAAATTC 
     3121 TGCAAGCACG CTTCCCAATG CCACGATATA TCAACACAGA GCACGGTGGC AGTCAGGCTC 
     3181 GATTCCTGTT GTCTAAAGTT AATCCTTCTC AGACACACAA TAACCTGTAT GCTTGGGGAC 
     3241 AGGAAACTGG AGCGCCCATC CTGACAGACG ATGTCAGCCT GCAGGTGTTC ATGGATCATT 
     3301 TGAAGAAGCT GGCTGTGTCC AGTGCCTCTA CGCGTACGCG GCCGCTCGAG CAGAAACTCA 
     3361 TCTCAGAAGA GGATCTGGCA GCAAATGATA TCCTGGATTA CAAGGATGAC GACGATAAGG 
     3421 TTTAAACGGC CGGCCGCGGT CATAGCTGTT TCCTGAACAG ATCCCGGGTG GCATCCCTGT 
     3481 GACCCCTCCC CAGTGCCTCT CCTGGCCCTG GAAGTTGCCA CTCCAGTGCC CACCAGCCTT 
     3541 GTCCTAATAA AATTAAGTTG CATCATTTTG TCTGACTAGG TGTCCTTCTA TAATATTATG 
     3601 GGGTGGAGGG GGGTGGTATG GAGCAAGGGG CAAGTTGGGA AGACAACCTG TAGGGCCTGC 
     3661 GGGGTCTATT GGGAACCAAG CTGGAGTGCA GTGGCACAAT CTTGGCTCAC TGCAATCTCC 
     3721 GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA 
     3781 TGCATGACCA GGCTCAGCTA ATTTTTGTTT TTTTGGTAGA GACGGGGTTT CACCATATTG 
     3841 GCCAGGCTGG TCTCCAACTC CTAATCTCAG GTGATCTACC CACCTTGGCC TCCCAAATTG 
     3901 CTGGGATTAC AGGCGTGAAC CACTGCTCCC TTCCCTGTCC TTCTGATTTT AAAATAACTA 
     3961 TACCAGCAGG AGGACGTCCA GACACAGCAT AGGCTACCTG GCCATGCCCA ACCGGTGGGA 
     4021 CATTTGAGTT GCTTGCTTGG CACTGTCCTC TCATGCGTTG GGTCCACTCA GTAGATGCCT 
     4081 GTTGAATTGG GTACGCGGCC AGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG 
     4141 GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA 
     4201 GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA 
     4261 CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG 
     4321 ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT 
     4381 TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG 
     4441 CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC 
     4501 CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT 
     4561 AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA 
     4621 TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC 
     4681 AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC 
     4741 TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT 
     4801 TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC 
     4861 TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT 
     4921 CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA 
     4981 ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC GACGTTGGCT GCGAGCCCTG GGCCTTCACC 
     5041 CGAACTTGGG GGGTGGGGTG GGGAAAAGGA AGAAACGCGG GCGTATTGGC CCCAATGGGG 
     5101 TCTCGGTGGG GTATCGACAG AGTGCCAGCC CTGGGACCGA ACCCCGCGTT TATGAACAAA 
     5161 CGACCCAACA CCGTGCGTTT TATTCTGTCT TTTTATTGCC GTCATAGCGC GGGTTCCTTC 
     5221 CGGTATTGTC TCCTTCCGTG TTTCAGTTAG CCTCCCCCTA GGGTGGGCGA AGAACTCCAG 
     5281 CATGAGATCC CCGCGCTGGA GGATCATCCA GCCGGCGTCC CGGAAAACGA TTCCGAAGCC 
     5341 CAACCTTTCA TAGAAGGCGG CGGTGGAATC GAAATCTCGT GATGGCAGGT TGGGCGTCGC 
     5401 TTGGTCGGTC ATTTCGAACC CCAGAGTCCC GCTCAGAAGA ACTCGTCAAG AAGGCGATAG 
     5461 AAGGCGATGC GCTGCGAATC GGGAGCGGCG ATACCGTAAA GCACGAGGAA GCGGTCAGCC 
     5521 CATTCGCCGC CAAGCTCTTC AGCAATATCA CGGGTAGCCA ACGCTATGTC CTGATAGCGA 
     5581 TCCGCCACAC CCAGCCGGCC ACAGTCGATG AATCCAGAAA AGCGGCCATT TTCCACCATG 
     5641 ATATTCGGCA AGCAGGCATC GCCATGGGTC ACGACGAGAT CCTCGCCGTC GGGCATGCTC 
     5701 GCCTTGAGCC TGGCGAACAG TTCGGCTGGC GCGAGCCCCT GATGCTCTTC GTCCAGATCA 
     5761 TCCTGATCGA CAAGACCGGC TTCCATCCGA GTACGTGCTC GCTCGATGCG ATGTTTCGCT 
     5821 TGGTGGTCGA ATGGGCAGGT AGCCGGATCA AGCGTATGCA GCCGCCGCAT TGCATCAGCC 
     5881 ATGATGGATA CTTTCTCGGC AGGAGCAAGG TGAGATGACA GGAGATCCTG CCCCGGCACT 
     5941 TCGCCCAATA GCAGCCAGTC CCTTCCCGCT TCAGTGACAA CGTCGAGCAC AGCTGCGCAA 
     6001 GGAACGCCCG TCGTGGCCAG CCACGATAGC CGCGCTGCCT CGTCTTGCAG TTCATTCAGG 
     6061 GCACCGGACA GGTCGGTCTT GACAAAAAGA ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC 
     6121 ACGGCGGCAT CAGAGCAGCC GATTGTCTGT TGTGCCCAGT CATAGCCGAA TAGCCTCTCC 
     6181 ACCCAAGCGG CCGGAGAACC TGCGTGCAAT CCATCTTGTT CAATCATGCG AAACGATCCT 
     6241 CATCCTGTCT CTTGATCGAT CTTTGCAAAA GCCTAGGCCT CCAAAAAAGC CTCCTCACTA 
     6301 CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC CTCGGCCTCT GCATAAATAA AAAAAATTAG 
     6361 TCAGCCATGG GGCGGAGAAT GGGCGGAACT GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT 
     6421 TAGGGGCGGG ACTATGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     6481 TGGGGAGCCT GGGGACTTTC CACACCTGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     6541 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCCT AACTGACACA CATTCCACAG 
     6601 CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT 
     6661 TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT 
     6721 CCGCGCACAT TTCCCCGAAA AGTGCCACCT GACGCGCCCT GTAGCGGCGC ATTAAGCGCG 
     6781 GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT 
     6841 CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA 
     6901 AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA 
     6961 CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT 
     7021 TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC 
     7081 AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG 
     7141 TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TT
//