LOCUS       RR217164                7128 bp    DNA     circular  SYN 
DEFINITION  Adam10 (myc-DDK-tagged) - Rat ADAM metallopeptidase domain 10 (Adam10)
ACCESSION   NM_019254
VERSION     NM_019254.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3285..3376 
                     /label="Myc-DDK"  
     misc_feature    3406..3991 
                     /label="PolyA signal"  
     misc_feature    3463..3482 
                     /label="Restriction Enzyme"  
     misc_feature    4267..4939 
                     /label="ColE1"  
     misc_feature    5369..6163 
                     /label="Kan/Neo"  
     misc_feature    6198..6500 
                     /label="SV40 ori"  
     misc_feature    6586..7063 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3267..3273 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3267 
                     /label="Adam10"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGTGTTGCCG ACAGTGTTAA TTCTGCTCCT CTCCTGGGCG GCGGGGCTGG 
     1081 GAGGTCAGTA TGGAAATCCT TTAAATAAAT ATATTAGACA TTATGAAGGA TTATCTTACA 
     1141 ATGTGGATTC ATTACACCAA AAACACCAGC GTGCCAAACG AGCAGTCTCA CACGAGGACC 
     1201 AGTTTTTACT TCTAGATTTC CATGCTCATG GAAGACAATT CAACCTACGA ATGAAGAGGG 
     1261 ACACTTCCCT TTTTAGTGAT GAATTTAAAG TAGAAACGTC AAATAAAGTA CTTGATTATG 
     1321 ATACCTCTCA TATTTACACT GGACATATTT ATGGTGAAGA AGGAAGCTTT AGTCATGGGT 
     1381 CTGTCGTTGA TGGAAGATTT GAAGGTTTCA TCCAGACTCG GGGTGGCACA TTTTATATTG 
     1441 AGCCAGCAGA GAGATATATT AAAGACCGAA TCCTGCCGTT TCACTCTGTC ATTTATCATG 
     1501 AAGATGATAT TAACTATCCC CATAAATACG GCCCACAGGG GGGCTGTGCA GATCACTCCG 
     1561 TCTTTGAAAG GATGAGGAAA TACCAAATGA CTGGAGTAGA GGAAGGAACT CGGGCACATT 
     1621 CAGAGGAGCA TGCTGCTAGT ATGGGTCCTG AGCTCCTGAG GAAAAAGCGC ACAACTCTGG 
     1681 CTGAGAGAAA TACTTGTCAG CTCTATATCC AGACAGATCA CCTGTTCTTT AAATACTATG 
     1741 GAACACGAGA AGCTGTGATT GCTCAGATAT CCAGTCATGT TAAAGCAATT GATACAATTT 
     1801 ACCAGACTAC AGACTTCTCC GGAATCCGTA ACATCAGCTT TATGGTGAAA CGCATAAGAA 
     1861 TCAATACAAC ATCTGATGAA AAAGATCCTA CAAATCCTTT CCGTTTCCCA AATATTGGTG 
     1921 TGGAGAAGTT CCTGGAGTTG AATTCTGAGC AGAATCATGA TGACTACTGC TTGGCCTATG 
     1981 TCTTCACGGA CCGGGATTTT GATGATGGTG TTCTTGGTCT GGCATGGGTT GGAGCACCTT 
     2041 CAGGAAGCTC TGGAGGAATA TGTGAGAAAA GCAAGTTGTA TTCAGATGGC AAGAAGAAGT 
     2101 CATTGAACAC GGGCATCATT ACTGTTCAGA ACTATGGCTC TCACGTGCCT CCCAAAGTCT 
     2161 CTCATATTAC TTTTGCTCAT GAAGTTGGAC ATAACTTTGG ATCTCCACAT GATTCTGGAA 
     2221 CAGAGTGCAC TCCAGGAGAG TCTAAGAACT TAGGACAAAA AGAAAATGGC AATTACATCA 
     2281 TGTATGCGAG AGCAACGTCT GGGGACAAAC TTAACAACAA CAAATTTTCA CTCTGCAGCA 
     2341 TTAGGAACAT AAGCCAAGTG CTTGAGAAGA AGAGGAACAA CTGTTTTGTT GAATCTGGCC 
     2401 AGCCTATCTG TGGAAATGGG ATGGTGGAGC AAGGGGAAGA GTGTGACTGT GGCTACAGTG 
     2461 ACCAATGCAA AGATGAGTGC TGCTTCGATG CAAACCAGCC AGAGGGGAAG AAATGCAAGC 
     2521 TGAAGCCTGG GAAGCAGTGC AGTCCAAGTC AAGGACCCTG CTGTACAGCA CAGTGTGCAT 
     2581 TCAAGTCAAA GTCTGAGAAA TGTCGGGATG ATTCTGACTG TGCAAAGGAA GGGATATGCA 
     2641 ATGGCTTCAC AGCCCTTTGC CCAGCATCTG ATCCCAAGCC CAACTTTACA GACTGTAACA 
     2701 GGCATACACA AGTGTGCATT AATGGGCAAT GTGCAGGTTC TATCTGTGAG AAGTATGACT 
     2761 TGGAGGAGTG CACCTGTGCC AGCTCTGATG GCAAAGATGA TAAGGAATTA TGTCATGTTT 
     2821 GCTGCATGAA GAAAATGGCC CCATCAACTT GTGCCAGTAC AGGCTCTTTG CAGTGGAACA 
     2881 AGCAGTTCAA TGGTCGGACT ATCACTCTGC AGCCAGGCTC TCCATGTAAT GACTTCAGAG 
     2941 GCTACTGTGA TGTTTTCATG CGGTGCAGAT TAGTAGATGC TGATGGCCCT CTAGCTAGGC 
     3001 TGAAAAAAGC AATTTTTAGT CCACAACTCT ATGAAAACAT TGCTGAGTGG ATTGTGGCTC 
     3061 ACTGGTGGGC AGTACTGCTT ATGGGAATTG CCCTGATCAT GCTAATGGCT GGATTTATCA 
     3121 AGATTTGCAG TGTTCACACT CCAAGTAGTA ATCCAAAGTT GCCTCCTCCT AAACCACTTC 
     3181 CAGGCACTTT AAAGAGGAGG AGACCGCCAC AGCCCATTCA GCAGCCCCCG CGTCAGAGGC 
     3241 CCCGGGAGAG TTATCAAATG GGACACATGC GACGCACGCG TACGCGGCCG CTCGAGCAGA 
     3301 AACTCATCTC AGAAGAGGAT CTGGCAGCAA ATGATATCCT GGATTACAAG GATGACGACG 
     3361 ATAAGGTTTA AACGGCCGGC CGCGGTCATA GCTGTTTCCT GAACAGATCC CGGGTGGCAT 
     3421 CCCTGTGACC CCTCCCCAGT GCCTCTCCTG GCCCTGGAAG TTGCCACTCC AGTGCCCACC 
     3481 AGCCTTGTCC TAATAAAATT AAGTTGCATC ATTTTGTCTG ACTAGGTGTC CTTCTATAAT 
     3541 ATTATGGGGT GGAGGGGGGT GGTATGGAGC AAGGGGCAAG TTGGGAAGAC AACCTGTAGG 
     3601 GCCTGCGGGG TCTATTGGGA ACCAAGCTGG AGTGCAGTGG CACAATCTTG GCTCACTGCA 
     3661 ATCTCCGCCT CCTGGGTTCA AGCGATTCTC CTGCCTCAGC CTCCCGAGTT GTTGGGATTC 
     3721 CAGGCATGCA TGACCAGGCT CAGCTAATTT TTGTTTTTTT GGTAGAGACG GGGTTTCACC 
     3781 ATATTGGCCA GGCTGGTCTC CAACTCCTAA TCTCAGGTGA TCTACCCACC TTGGCCTCCC 
     3841 AAATTGCTGG GATTACAGGC GTGAACCACT GCTCCCTTCC CTGTCCTTCT GATTTTAAAA 
     3901 TAACTATACC AGCAGGAGGA CGTCCAGACA CAGCATAGGC TACCTGGCCA TGCCCAACCG 
     3961 GTGGGACATT TGAGTTGCTT GCTTGGCACT GTCCTCTCAT GCGTTGGGTC CACTCAGTAG 
     4021 ATGCCTGTTG AATTGGGTAC GCGGCCAGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT 
     4081 AATACGGTTA TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA 
     4141 GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC 
     4201 CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT 
     4261 ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT 
     4321 GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG 
     4381 CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA 
     4441 CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA 
     4501 CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC 
     4561 GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG 
     4621 AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG 
     4681 TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA 
     4741 GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC 
     4801 TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG 
     4861 GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA 
     4921 TGAGTAACCT GAGGCTATGG CAGGGCCTGC CGCCCCGACG TTGGCTGCGA GCCCTGGGCC 
     4981 TTCACCCGAA CTTGGGGGGT GGGGTGGGGA AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA 
     5041 ATGGGGTCTC GGTGGGGTAT CGACAGAGTG CCAGCCCTGG GACCGAACCC CGCGTTTATG 
     5101 AACAAACGAC CCAACACCGT GCGTTTTATT CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT 
     5161 TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA 
     5221 CTCCAGCATG AGATCCCCGC GCTGGAGGAT CATCCAGCCG GCGTCCCGGA AAACGATTCC 
     5281 GAAGCCCAAC CTTTCATAGA AGGCGGCGGT GGAATCGAAA TCTCGTGATG GCAGGTTGGG 
     5341 CGTCGCTTGG TCGGTCATTT CGAACCCCAG AGTCCCGCTC AGAAGAACTC GTCAAGAAGG 
     5401 CGATAGAAGG CGATGCGCTG CGAATCGGGA GCGGCGATAC CGTAAAGCAC GAGGAAGCGG 
     5461 TCAGCCCATT CGCCGCCAAG CTCTTCAGCA ATATCACGGG TAGCCAACGC TATGTCCTGA 
     5521 TAGCGATCCG CCACACCCAG CCGGCCACAG TCGATGAATC CAGAAAAGCG GCCATTTTCC 
     5581 ACCATGATAT TCGGCAAGCA GGCATCGCCA TGGGTCACGA CGAGATCCTC GCCGTCGGGC 
     5641 ATGCTCGCCT TGAGCCTGGC GAACAGTTCG GCTGGCGCGA GCCCCTGATG CTCTTCGTCC 
     5701 AGATCATCCT GATCGACAAG ACCGGCTTCC ATCCGAGTAC GTGCTCGCTC GATGCGATGT 
     5761 TTCGCTTGGT GGTCGAATGG GCAGGTAGCC GGATCAAGCG TATGCAGCCG CCGCATTGCA 
     5821 TCAGCCATGA TGGATACTTT CTCGGCAGGA GCAAGGTGAG ATGACAGGAG ATCCTGCCCC 
     5881 GGCACTTCGC CCAATAGCAG CCAGTCCCTT CCCGCTTCAG TGACAACGTC GAGCACAGCT 
     5941 GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC GATAGCCGCG CTGCCTCGTC TTGCAGTTCA 
     6001 TTCAGGGCAC CGGACAGGTC GGTCTTGACA AAAAGAACCG GGCGCCCCTG CGCTGACAGC 
     6061 CGGAACACGG CGGCATCAGA GCAGCCGATT GTCTGTTGTG CCCAGTCATA GCCGAATAGC 
     6121 CTCTCCACCC AAGCGGCCGG AGAACCTGCG TGCAATCCAT CTTGTTCAAT CATGCGAAAC 
     6181 GATCCTCATC CTGTCTCTTG ATCGATCTTT GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC 
     6241 TCACTACTTC TGGAATAGCT CAGAGGCCGA GGCGGCCTCG GCCTCTGCAT AAATAAAAAA 
     6301 AATTAGTCAG CCATGGGGCG GAGAATGGGC GGAACTGGGC GGAGTTAGGG GCGGGATGGG 
     6361 CGGAGTTAGG GGCGGGACTA TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     6421 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCTGGTTGCT GACTAATTGA GATGCATGCT 
     6481 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCCTAACT GACACACATT 
     6541 CCACAGCTGG TTCTTTCCGC CTCAGGACTC TTCCTTTTTC AATATTATTG AAGCATTTAT 
     6601 CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA 
     6661 GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTGACG CGCCCTGTAG CGGCGCATTA 
     6721 AGCGCGGCGG GTGTGGTGGT TACGCGCAGC GTGACCGCTA CACTTGCCAG CGCCCTAGCG 
     6781 CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT CTCGCCACGT TCGCCGGCTT TCCCCGTCAA 
     6841 GCTCTAAATC GGGGGCTCCC TTTAGGGTTC CGATTTAGTG CTTTACGGCA CCTCGACCCC 
     6901 AAAAAACTTG ATTAGGGTGA TGGTTCACGT AGTGGGCCAT CGCCCTGATA GACGGTTTTT 
     6961 CGCCCTTTGA CGTTGGAGTC CACGTTCTTT AATAGTGGAC TCTTGTTCCA AACTGGAACA 
     7021 ACACTCAACC CTATCTCGGT CTATTCTTTT GATTTATAAG GGATTTTGCC GATTTCGGCC 
     7081 TATTGGTTAA AAAATGAGCT GATTTAACAA AAATTTAACG CGAATTTT
//