LOCUS       RR217047                6834 bp    DNA     circular  SYN 
DEFINITION  Nrg1 (myc-DDK-tagged) - Rat neuregulin 1 (Nrg1), transcript variant 14
ACCESSION   NM_001271130
VERSION     NM_001271130.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    2991..3082 
                     /label="Myc-DDK"  
     misc_feature    3112..3697 
                     /label="PolyA signal"  
     misc_feature    3169..3188 
                     /label="Restriction Enzyme"  
     misc_feature    3973..4645 
                     /label="ColE1"  
     misc_feature    5075..5869 
                     /label="Kan/Neo"  
     misc_feature    5904..6206 
                     /label="SV40 ori"  
     misc_feature    6292..6769 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    2973..2979 
                     /label="Restriction Enzyme"  
     misc_feature    1020..2973 
                     /label="Nrg1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAGCCGGAT GCCAACAGCA GCGGCCGCGC GCCGCCCGCC TTCCGAGCCT 
     1081 CGTTTCCCCC ACTGGAGACT GGCCGCAACC TCAAGAAGGA GGTCAGCCGG GTGTTGTGCA 
     1141 AGCGGTGCGC ACTGCCTCCC AGATTGAAAG AAATGAAGAG CCAGGAGTCA GCTGCAGGCT 
     1201 CCAAGCTAGT GCTCCGGTGC GAAACCAGCT CCGAGTACTC CTCACTCAGA TTCAAATGGT 
     1261 TCAAGAATGG GAACGAGCTG AACCGCAAAA ATAAACCAGA AAACATCAAG ATACAGAAGA 
     1321 AGCCAGGGAA GTCAGAGCTT CGAATTAACA AAGCATCCCT GGCTGACTCT GGAGAGTATA 
     1381 TGTGCAAAGT GATCAGCAAG TTAGGAAATG ACAGTGCCTC TGCCAACATC ACCATTGTTG 
     1441 AGTCAAACGA GTTCATCACT GGCATGCCAG CCTCGACTGA GACAGCCTAT GTGTCCTCAG 
     1501 AGTCTCCCAT TAGAATCTCA GTTTCAACAG AAGGCGCAAA CACTTCTTCA TCCACATCGA 
     1561 CATCCACGAC TGGGACCAGC CATCTCATAA AGTGCGCGGA GAAGGAGAAA ACTTTCTGTG 
     1621 TGAATGGGGG CGAGTGCTTC ACGGTGAAGG ACCTGTCAAA CCCGTCAAGA TACTTGTGCA 
     1681 AGTGCCCAAA TGAGTTTACT GGTGATCGTT GCCAAAACTA CGTAATGGCC AGCTTCTACA 
     1741 AGCATCTTGG GATTGAATTT ATGGAAGCGG AGGAACTCTA CCAGAAGAGG GTGCTGACAA 
     1801 TTACTGGCAT CTGTATCGCC CTGCTGGTGG TCGGCATCAT GTGTGTGGTG GCCTACTGCA 
     1861 AAACCAAGAA GCAGCGGCAG AAGCTTCATG ATCGGCTTCG GCAGAGTCTT CGGTCAGAAC 
     1921 GGAGCAACCT GGTGAACATA GCGAATGGGC CTCACCACCC AAACCCACCG CCAGAGAACG 
     1981 TGCAGCTGGT GAATCAATAC GTATCTAAAA ACGTCATCTC CAGTGAGCAT ATTGTTGAGA 
     2041 GAGAAGTGGA GACTTCCTTT TCCACCAGTC ATTACACTTC CACAGCCCAT CACTCCACGA 
     2101 CTGTCACCCA GACTCCTAGT CACAGCTGGA GTAATGGGCA CACGGAGAGC ATCATTTCAG 
     2161 AAAGCAACTC CGTAATCATG ATGTCTTCGG TAGAGAACAG CAGGCACAGC AGTCCCGCCG 
     2221 GGGGCCCACG AGGACGTCTT CATGGCCTGG GAGGCCCTCG TGATAACAGC TTCCTCAGGC 
     2281 ATGCCAGAGA AACCCCTGAC TCCTACAGAG ACTCTCCTCA TAGCGAAAGG TATGTATCAG 
     2341 CCATGACCAC CCCGGCTCGT ATGTCACCTG TAGATTTCCA CACGCCAAGC TCCCCTAAAT 
     2401 CGCCCCCTTC GGAAATGTCT CCACCCGTGT CCAGCATGAC GGTGTCAATG CCCTCTGTGG 
     2461 CAGTCAGCCC CTTTGTGGAA GAAGAGAGGC CTCTGCTGCT TGTGACGCCA CCAAGGCTAC 
     2521 GGGAGAAGAA ATATGATCAT CACCCCCAGC AACTCAACTC CTTTCATCAC AACCCTGCAC 
     2581 ATCAGAGTAC CAGCCTCCCC CCTAGCCCAC TGAGGATAGT GGAGGATGAG GAGTACGAGA 
     2641 CGACCCAGGA GTATGAGTCA GTTCAAGAGC CCGTTAAGAA AGTCACCAAT AGCCGGCGGG 
     2701 CCAAAAGAAC CAAGCCCAAT GGCCACATTG CCAATAGGTT GGAAATGGAC AGCAACACAA 
     2761 GTTCTGTGAG CAGTAACTCA GAAAGTGAGA CAGAAGACGA AAGAGTAGGT GAAGACACAC 
     2821 CATTCCTGGG CATACAGAAC CCCCTGGCAG CCAGCCTTGA GGTGGCCCCT GCCTTCCGTC 
     2881 TGGCTGAGAG CAGGACTAAC CCAGCAGGCC GCTTCTCCAC ACAGGAGGAA TTACAGGCCA 
     2941 GGCTGTCTAG TGTAATCGCT AACCAAGACC CTATTGCTGT AACGCGTACG CGGCCGCTCG 
     3001 AGCAGAAACT CATCTCAGAA GAGGATCTGG CAGCAAATGA TATCCTGGAT TACAAGGATG 
     3061 ACGACGATAA GGTTTAAACG GCCGGCCGCG GTCATAGCTG TTTCCTGAAC AGATCCCGGG 
     3121 TGGCATCCCT GTGACCCCTC CCCAGTGCCT CTCCTGGCCC TGGAAGTTGC CACTCCAGTG 
     3181 CCCACCAGCC TTGTCCTAAT AAAATTAAGT TGCATCATTT TGTCTGACTA GGTGTCCTTC 
     3241 TATAATATTA TGGGGTGGAG GGGGGTGGTA TGGAGCAAGG GGCAAGTTGG GAAGACAACC 
     3301 TGTAGGGCCT GCGGGGTCTA TTGGGAACCA AGCTGGAGTG CAGTGGCACA ATCTTGGCTC 
     3361 ACTGCAATCT CCGCCTCCTG GGTTCAAGCG ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG 
     3421 GGATTCCAGG CATGCATGAC CAGGCTCAGC TAATTTTTGT TTTTTTGGTA GAGACGGGGT 
     3481 TTCACCATAT TGGCCAGGCT GGTCTCCAAC TCCTAATCTC AGGTGATCTA CCCACCTTGG 
     3541 CCTCCCAAAT TGCTGGGATT ACAGGCGTGA ACCACTGCTC CCTTCCCTGT CCTTCTGATT 
     3601 TTAAAATAAC TATACCAGCA GGAGGACGTC CAGACACAGC ATAGGCTACC TGGCCATGCC 
     3661 CAACCGGTGG GACATTTGAG TTGCTTGCTT GGCACTGTCC TCTCATGCGT TGGGTCCACT 
     3721 CAGTAGATGC CTGTTGAATT GGGTACGCGG CCAGCGGCGA GCGGTATCAG CTCACTCAAA 
     3781 GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA 
     3841 AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT 
     3901 CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC 
     3961 AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC 
     4021 GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC 
     4081 TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG 
     4141 TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA 
     4201 GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG 
     4261 CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA 
     4321 CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG 
     4381 AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG 
     4441 CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC 
     4501 GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC 
     4561 AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG 
     4621 TATATATGAG TAACCTGAGG CTATGGCAGG GCCTGCCGCC CCGACGTTGG CTGCGAGCCC 
     4681 TGGGCCTTCA CCCGAACTTG GGGGGTGGGG TGGGGAAAAG GAAGAAACGC GGGCGTATTG 
     4741 GCCCCAATGG GGTCTCGGTG GGGTATCGAC AGAGTGCCAG CCCTGGGACC GAACCCCGCG 
     4801 TTTATGAACA AACGACCCAA CACCGTGCGT TTTATTCTGT CTTTTTATTG CCGTCATAGC 
     4861 GCGGGTTCCT TCCGGTATTG TCTCCTTCCG TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC 
     4921 GAAGAACTCC AGCATGAGAT CCCCGCGCTG GAGGATCATC CAGCCGGCGT CCCGGAAAAC 
     4981 GATTCCGAAG CCCAACCTTT CATAGAAGGC GGCGGTGGAA TCGAAATCTC GTGATGGCAG 
     5041 GTTGGGCGTC GCTTGGTCGG TCATTTCGAA CCCCAGAGTC CCGCTCAGAA GAACTCGTCA 
     5101 AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA TCGGGAGCGG CGATACCGTA AAGCACGAGG 
     5161 AAGCGGTCAG CCCATTCGCC GCCAAGCTCT TCAGCAATAT CACGGGTAGC CAACGCTATG 
     5221 TCCTGATAGC GATCCGCCAC ACCCAGCCGG CCACAGTCGA TGAATCCAGA AAAGCGGCCA 
     5281 TTTTCCACCA TGATATTCGG CAAGCAGGCA TCGCCATGGG TCACGACGAG ATCCTCGCCG 
     5341 TCGGGCATGC TCGCCTTGAG CCTGGCGAAC AGTTCGGCTG GCGCGAGCCC CTGATGCTCT 
     5401 TCGTCCAGAT CATCCTGATC GACAAGACCG GCTTCCATCC GAGTACGTGC TCGCTCGATG 
     5461 CGATGTTTCG CTTGGTGGTC GAATGGGCAG GTAGCCGGAT CAAGCGTATG CAGCCGCCGC 
     5521 ATTGCATCAG CCATGATGGA TACTTTCTCG GCAGGAGCAA GGTGAGATGA CAGGAGATCC 
     5581 TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG TCCCTTCCCG CTTCAGTGAC AACGTCGAGC 
     5641 ACAGCTGCGC AAGGAACGCC CGTCGTGGCC AGCCACGATA GCCGCGCTGC CTCGTCTTGC 
     5701 AGTTCATTCA GGGCACCGGA CAGGTCGGTC TTGACAAAAA GAACCGGGCG CCCCTGCGCT 
     5761 GACAGCCGGA ACACGGCGGC ATCAGAGCAG CCGATTGTCT GTTGTGCCCA GTCATAGCCG 
     5821 AATAGCCTCT CCACCCAAGC GGCCGGAGAA CCTGCGTGCA ATCCATCTTG TTCAATCATG 
     5881 CGAAACGATC CTCATCCTGT CTCTTGATCG ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA 
     5941 GCCTCCTCAC TACTTCTGGA ATAGCTCAGA GGCCGAGGCG GCCTCGGCCT CTGCATAAAT 
     6001 AAAAAAAATT AGTCAGCCAT GGGGCGGAGA ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG 
     6061 GATGGGCGGA GTTAGGGGCG GGACTATGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     6121 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCTG GTTGCTGACT AATTGAGATG 
     6181 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC CTAACTGACA 
     6241 CACATTCCAC AGCTGGTTCT TTCCGCCTCA GGACTCTTCC TTTTTCAATA TTATTGAAGC 
     6301 ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA 
     6361 CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGCGCC CTGTAGCGGC 
     6421 GCATTAAGCG CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC 
     6481 CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC 
     6541 CGTCAAGCTC TAAATCGGGG GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC 
     6601 GACCCCAAAA AACTTGATTA GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG 
     6661 GTTTTTCGCC CTTTGACGTT GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT 
     6721 GGAACAACAC TCAACCCTAT CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT 
     6781 TCGGCCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA TTTT
//