LOCUS       RR217040                6825 bp    DNA     circular  SYN 
DEFINITION  Inpp5e (myc-DDK-tagged) - Rat inositol polyphosphate-5-phosphatase E (Inpp5e)
ACCESSION   NM_053632
VERSION     NM_053632.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    2982..3073 
                     /label="Myc-DDK"  
     misc_feature    3103..3688 
                     /label="PolyA signal"  
     misc_feature    3160..3179 
                     /label="Restriction Enzyme"  
     misc_feature    3964..4636 
                     /label="ColE1"  
     misc_feature    5066..5860 
                     /label="Kan/Neo"  
     misc_feature    5895..6197 
                     /label="SV40 ori"  
     misc_feature    6283..6760 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    2964..2970 
                     /label="Restriction Enzyme"  
     misc_feature    1020..2964 
                     /label="Inpp5e"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCCGTCCAAG TCCGCTTGCC TGCGTCACAC GGAGGCACCT GGGCAGCTAG 
     1081 AAGGCAGAAT GCTCCAGGGA CAGCTTTCGA ACCCCGAAAA GAAGCTCATC CCAACATCAG 
     1141 CCTCCTTACC AGCTGCTGAC TCTCAGAGTT CACAGACAAA CTCCATGCCT CCTTTAAGCA 
     1201 TGCCAGCAAA ACCAAGCAAC CAGAACCTAC AAGCCAAGGC AAACCTCATC ACCCCACAGC 
     1261 CACCCATTAG ACCCAAGTTG GAGAGAACCC TGTCCCTCGA TGACAAGGGC TGGAGAAGGA 
     1321 GGCGTTTCCG GGGTAGTCAG GAGGATCTGA CCGTCCAGAA TGGGGCCAGT CCCTGCAGGG 
     1381 GTTCCTTGCA GGACTCAGTA GCCCAGTCTC CTGCCTACAG TCGTCCCCTG CCCTGCCTCA 
     1441 GTACATCCTT ACAGGAGATA CCTAAGCCCC GAAGGGCCAC AGGCAGCGAG GGAGGGAGCC 
     1501 CATCCTTGTG GAGTGACTGT CTTTCTGGAA TGATCAGCAC CTCCTTGGAC CTCCTGCACA 
     1561 GAGAGGCTGC CTCAGGTGGG CCCCACTCCA GGCTGGCAAG TTTGCGTGCT ACACACACAC 
     1621 CGCCAGCTAT GGACCTCAAC ATAGCCTCCA GCTCCCTGAG AACAGCAAAC AAGGTTGACC 
     1681 CTGAGCACAC GGACTACAAG CTCCGCATGC AGAACAGACT GGTCAGGGCC CACAGCAACC 
     1741 TGGGCCCTAG CAGACCCCGG AGTCCCCTGG CTGGTGACGA CCACTCCATT CACTCAGCCA 
     1801 GATCCTCTTT CAGCCTTCTG GCCCCCATCC GCACCAAGGA CATCAGGAGC AGGAGCTATC 
     1861 TGGAGGGAAG TCTTCTGGCC AGTGGGGCCC TGCTAGGAGC AGACGAGCTG GCCAGGTACT 
     1921 TCCCAGACCG AAACATGGCT CTTTTCGTGG CTACCTGGAA CATGCAGGGC CAGAAGGAGC 
     1981 TTCCGGCAAG CCTGGATGAG TTTCTGCTTC CCACCGAGGC TGACTACACA CAGGACCTGT 
     2041 ATGTCATTGG CGTTCAGGAG GGCTGCTCTG ACAGGCGGGA ATGGGAGACA CGCTTGCAGG 
     2101 AGACACTGGG CCCTCAGTAT GTACTGCTGT CATCAGCAGC ACATGGGGTC CTGTACATGT 
     2161 CCCTGTTTAT CCGTAGGGAC CTCATCTGGT TCTGCTCAGA GGTCGAGTAC TCCACAGTAA 
     2221 CTACACGCAT CGTGTCTCAG ATCAAGACCA AAGGGGCCCT GGGCGTCAGC TTCACCTTTT 
     2281 TTGGCACCTC CTTCCTCTTC ATCACATCTC ACTTCACCTC TGGAGATGGG AAGGTAGCAG 
     2341 AGCGGCTACT GGACTACAAC AGAACCATCC AAGCCCTCGC CTTGCCCCGG AATGTGCCAG 
     2401 ACACAAACCC CTACCGCTCT AGTGCAGGGG ATGTCACTAC CCGGTTTGAT GAGGTCTTCT 
     2461 GGTTTGGGGA CTTCAACTTC CGCCTAAGTG GTGGACGAGT GGCTGTGGAG GCCTTCCTGA 
     2521 AGCAGGACCC AGAGGTGGAT GTGCTGGCAC TCCTGCAACA TGACCAGCTC ACCCGGGAGA 
     2581 TGAAGAAAGG GTCCATCTTC AAGGGCTTTG AGGAGGCAGA AATTCACTTT CTCCCATCCT 
     2641 ACAAGTTTGA CATTGGGAAG GACACCTACG ACAGCACCTC CAAGCAAAGG ACACCCTCCT 
     2701 ACACAGACCG AGTCCTATAC AAAAGCCGTC ACAAGGGTGA CATCTGTCCC ATGAAGTATT 
     2761 CCTCTTGTCC TGGGATCAAG ACTTCAGACC ACCGTCCTGT GTATGGCTTG TTCCGGGTGA 
     2821 AAGTGAGGCC AGGACGAGAC AACATCCCGT TAGCTGCCGG CAAGTTTGAC CGAGAACTGT 
     2881 ACTTGATAGG AATTAAAAGA CGAATTTCGA AAGAGATTCA GAGACAAGAA GCACTGAAGA 
     2941 GTCAGAGCTC CAGTGCTGTG TGCACTGTGT CTACGCGTAC GCGGCCGCTC GAGCAGAAAC 
     3001 TCATCTCAGA AGAGGATCTG GCAGCAAATG ATATCCTGGA TTACAAGGAT GACGACGATA 
     3061 AGGTTTAAAC GGCCGGCCGC GGTCATAGCT GTTTCCTGAA CAGATCCCGG GTGGCATCCC 
     3121 TGTGACCCCT CCCCAGTGCC TCTCCTGGCC CTGGAAGTTG CCACTCCAGT GCCCACCAGC 
     3181 CTTGTCCTAA TAAAATTAAG TTGCATCATT TTGTCTGACT AGGTGTCCTT CTATAATATT 
     3241 ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG GGGCAAGTTG GGAAGACAAC CTGTAGGGCC 
     3301 TGCGGGGTCT ATTGGGAACC AAGCTGGAGT GCAGTGGCAC AATCTTGGCT CACTGCAATC 
     3361 TCCGCCTCCT GGGTTCAAGC GATTCTCCTG CCTCAGCCTC CCGAGTTGTT GGGATTCCAG 
     3421 GCATGCATGA CCAGGCTCAG CTAATTTTTG TTTTTTTGGT AGAGACGGGG TTTCACCATA 
     3481 TTGGCCAGGC TGGTCTCCAA CTCCTAATCT CAGGTGATCT ACCCACCTTG GCCTCCCAAA 
     3541 TTGCTGGGAT TACAGGCGTG AACCACTGCT CCCTTCCCTG TCCTTCTGAT TTTAAAATAA 
     3601 CTATACCAGC AGGAGGACGT CCAGACACAG CATAGGCTAC CTGGCCATGC CCAACCGGTG 
     3661 GGACATTTGA GTTGCTTGCT TGGCACTGTC CTCTCATGCG TTGGGTCCAC TCAGTAGATG 
     3721 CCTGTTGAAT TGGGTACGCG GCCAGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT 
     3781 ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA 
     3841 AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC 
     3901 TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA 
     3961 AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC 
     4021 GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC 
     4081 ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA 
     4141 ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC 
     4201 GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG 
     4261 GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG 
     4321 AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG 
     4381 CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA 
     4441 GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA 
     4501 CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT 
     4561 CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA 
     4621 GTAACCTGAG GCTATGGCAG GGCCTGCCGC CCCGACGTTG GCTGCGAGCC CTGGGCCTTC 
     4681 ACCCGAACTT GGGGGGTGGG GTGGGGAAAA GGAAGAAACG CGGGCGTATT GGCCCCAATG 
     4741 GGGTCTCGGT GGGGTATCGA CAGAGTGCCA GCCCTGGGAC CGAACCCCGC GTTTATGAAC 
     4801 AAACGACCCA ACACCGTGCG TTTTATTCTG TCTTTTTATT GCCGTCATAG CGCGGGTTCC 
     4861 TTCCGGTATT GTCTCCTTCC GTGTTTCAGT TAGCCTCCCC CTAGGGTGGG CGAAGAACTC 
     4921 CAGCATGAGA TCCCCGCGCT GGAGGATCAT CCAGCCGGCG TCCCGGAAAA CGATTCCGAA 
     4981 GCCCAACCTT TCATAGAAGG CGGCGGTGGA ATCGAAATCT CGTGATGGCA GGTTGGGCGT 
     5041 CGCTTGGTCG GTCATTTCGA ACCCCAGAGT CCCGCTCAGA AGAACTCGTC AAGAAGGCGA 
     5101 TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG GCGATACCGT AAAGCACGAG GAAGCGGTCA 
     5161 GCCCATTCGC CGCCAAGCTC TTCAGCAATA TCACGGGTAG CCAACGCTAT GTCCTGATAG 
     5221 CGATCCGCCA CACCCAGCCG GCCACAGTCG ATGAATCCAG AAAAGCGGCC ATTTTCCACC 
     5281 ATGATATTCG GCAAGCAGGC ATCGCCATGG GTCACGACGA GATCCTCGCC GTCGGGCATG 
     5341 CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT GGCGCGAGCC CCTGATGCTC TTCGTCCAGA 
     5401 TCATCCTGAT CGACAAGACC GGCTTCCATC CGAGTACGTG CTCGCTCGAT GCGATGTTTC 
     5461 GCTTGGTGGT CGAATGGGCA GGTAGCCGGA TCAAGCGTAT GCAGCCGCCG CATTGCATCA 
     5521 GCCATGATGG ATACTTTCTC GGCAGGAGCA AGGTGAGATG ACAGGAGATC CTGCCCCGGC 
     5581 ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC GCTTCAGTGA CAACGTCGAG CACAGCTGCG 
     5641 CAAGGAACGC CCGTCGTGGC CAGCCACGAT AGCCGCGCTG CCTCGTCTTG CAGTTCATTC 
     5701 AGGGCACCGG ACAGGTCGGT CTTGACAAAA AGAACCGGGC GCCCCTGCGC TGACAGCCGG 
     5761 AACACGGCGG CATCAGAGCA GCCGATTGTC TGTTGTGCCC AGTCATAGCC GAATAGCCTC 
     5821 TCCACCCAAG CGGCCGGAGA ACCTGCGTGC AATCCATCTT GTTCAATCAT GCGAAACGAT 
     5881 CCTCATCCTG TCTCTTGATC GATCTTTGCA AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA 
     5941 CTACTTCTGG AATAGCTCAG AGGCCGAGGC GGCCTCGGCC TCTGCATAAA TAAAAAAAAT 
     6001 TAGTCAGCCA TGGGGCGGAG AATGGGCGGA ACTGGGCGGA GTTAGGGGCG GGATGGGCGG 
     6061 AGTTAGGGGC GGGACTATGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     6121 TGCTGGGGAG CCTGGGGACT TTCCACACCT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     6181 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CCTAACTGAC ACACATTCCA 
     6241 CAGCTGGTTC TTTCCGCCTC AGGACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG 
     6301 GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG 
     6361 GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGACGCGC CCTGTAGCGG CGCATTAAGC 
     6421 GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG ACCGCTACAC TTGCCAGCGC CCTAGCGCCC 
     6481 GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC GCCACGTTCG CCGGCTTTCC CCGTCAAGCT 
     6541 CTAAATCGGG GGCTCCCTTT AGGGTTCCGA TTTAGTGCTT TACGGCACCT CGACCCCAAA 
     6601 AAACTTGATT AGGGTGATGG TTCACGTAGT GGGCCATCGC CCTGATAGAC GGTTTTTCGC 
     6661 CCTTTGACGT TGGAGTCCAC GTTCTTTAAT AGTGGACTCT TGTTCCAAAC TGGAACAACA 
     6721 CTCAACCCTA TCTCGGTCTA TTCTTTTGAT TTATAAGGGA TTTTGCCGAT TTCGGCCTAT 
     6781 TGGTTAAAAA ATGAGCTGAT TTAACAAAAA TTTAACGCGA ATTTT
//