LOCUS       RR217035                6813 bp    DNA     circular  SYN 
DEFINITION  Chat (myc-DDK-tagged) - Rat choline O-acetyltransferase (Chat)
ACCESSION   NM_001170593
VERSION     NM_001170593.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    2970..3061 
                     /label="Myc-DDK"  
     misc_feature    3091..3676 
                     /label="PolyA signal"  
     misc_feature    3148..3167 
                     /label="Restriction Enzyme"  
     misc_feature    3952..4624 
                     /label="ColE1"  
     misc_feature    5054..5848 
                     /label="Kan/Neo"  
     misc_feature    5883..6185 
                     /label="SV40 ori"  
     misc_feature    6271..6748 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    2952..2958 
                     /label="Restriction Enzyme"  
     misc_feature    1020..2952 
                     /label="Chat"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCCCATCCTG GAAAAGGCTC CCCAAAAGAT GCCTGTAAAG GCTTCTAGCT 
     1081 GGGAGGAGCT GGACTTACCT AAATTACCAG TGCCCCCGCT GCAGCAAACC CTGGCCACCT 
     1141 ACCTACAGTG CATGCAGCAT CTGGTACCTG AAGAGCAGTT CAGGAAGAGC CAGGCCATTG 
     1201 TGAAGCGGTT TGGGGCCCCT GGTGGCCTGG GTGAGACCCT GCAGGAAAAG CTCTTGGAGA 
     1261 GACAGGAGAA GACAGCCAAT TGGGTCTCTG AATACTGGCT GAATGACATG TACCTGAACA 
     1321 ACCGCCTGGC CCTGCCAGTC AACTCCAGCC CTGCTGTGAT CTTTGCTCGG CAGCACTTCC 
     1381 AAGACACCAA TGACCAGCTA AGATTTGCAG CCTGCCTCAT CTCTGGTGTG CTTAGCTACA 
     1441 AAACTCTGCT GGACAGCCAC TCCCTTCCCA CTGACTGGGC CAAGGGGCAG CTCTCAGGGC 
     1501 AGCCCCTCTG TATGAAGCAA TATTACAGAC TCTTCTCCTC TTACCGGCTT CCTGGCCATA 
     1561 CCCAGGACAC ACTGGTGGCC CAGAAGAGCA GTATCATGCC CGAGCCTGAG CATGTCATCG 
     1621 TGGCCTGCTG CAACCAGTTC TTTGTCTTGG ATGTTGTCAT TAATTTCCGC CGTCTCAGTG 
     1681 AGGGTGATCT GTTCACTCAG TTGAGAAAGA TAGTCAAAAT GGCGTCCAAC GAGGATGAAC 
     1741 GCCTGCCTCC AATCGGCCTG CTGACGTCAG ACGGGAGGAG CGAGTGGGCC AAGGCCAGGA 
     1801 CGGTCCTCTT AAAAGACTCC ACCAACCGGG ACTCCCTGGA CATGATCGAG CGCTGCATCT 
     1861 GCCTGGTATG CCTGGATGGT CCAGGCACAG GAGAGCTCAG TGACACCCAC AGGGCCCTCC 
     1921 AACTCCTTCA CGGTGGAGGC TGCAGCCTGA ACGGAGCCAA TCGCTGGTAT GACAAGTCCC 
     1981 TGCAGTTCGT GGTGGGCCGA GATGGCACCT GCGGTGTGGT GTGTGAGCAT TCTCCATTTG 
     2041 ATGGCATCGT CCTGGTGCAG TGCACAGAGC ACCTGCTGAA ACATATGATG ACAAGCAACA 
     2101 AGAAGCTCGT CAGGGCTGAC TCAGTGAGTG AGCTCCCTGC CCCCAGAAGG CTGAGGTGGA 
     2161 AATGTTCCCC AGAAACCCAA GGCCATCTCG CCTCCTCAGC AGAGAAACTT CAAAGAATAG 
     2221 TAAAGAATCT GGATTTCATT GTTTACAAGT TTGACAACTA TGGAAAAACA TTTATCAAGA 
     2281 AGCAGAAATA CAGCCCCGAC GGCTTCATCC AGGTGGCCCT CCAGCTGGCT TACTACAGGC 
     2341 TTTACCAGAG ACTGGTGCCC ACCTATGAGA GCGCATCCAT CCGCCGCTTC CAGGAAGGTC 
     2401 GGGTGGACAA CATCAGATCA GCCACTCCAG AGGCTCTGGC TTTTGTGCAA GCCATGACTG 
     2461 ACCACAAGGC TGCCATGCCG GCTTCTGAGA AACTGCAGCT GCTGCAGACA GCCATGCAGG 
     2521 CCCAGACTGA GTACACAGTC ATGGCCATAA CCGGCATGGC CATTGACAAC CATCTTCTGG 
     2581 CACTGAGGGA GCTGGCCCGA GACCTGTGCA AAGAGCCACC TGAGATGTTC ATGGATGAAA 
     2641 CATACCTGAT GAGCAACCGG TTTGTCCTCT CCACCAGCCA GGTGCCCACA ACCATGGAGA 
     2701 TGTTCTGCTG TTATGGACCC GTGGTCCCCA ATGGCTATGG AGCCTGCTAC AACCCCCAGC 
     2761 CCGAGGCCAT CACCTTCTGC ATCTCCAGTT TTCACAGCTG CAAAGAGACC TCGTCTGTGG 
     2821 AGTTTGCGGA AGCTGTGGGA GCGAGCCTTG TTGACATGAG AGACCTCTGC AGTTCAAGGC 
     2881 AGCCTGCTGA CAGCAAGCCA CCAGCACCCA AGGAAAAAGC TAGGGGCCCA ACCAAGCCAA 
     2941 GCAATCTTGA CTCCAGCCAC ACGCGTACGC GGCCGCTCGA GCAGAAACTC ATCTCAGAAG 
     3001 AGGATCTGGC AGCAAATGAT ATCCTGGATT ACAAGGATGA CGACGATAAG GTTTAAACGG 
     3061 CCGGCCGCGG TCATAGCTGT TTCCTGAACA GATCCCGGGT GGCATCCCTG TGACCCCTCC 
     3121 CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC ACTCCAGTGC CCACCAGCCT TGTCCTAATA 
     3181 AAATTAAGTT GCATCATTTT GTCTGACTAG GTGTCCTTCT ATAATATTAT GGGGTGGAGG 
     3241 GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG AAGACAACCT GTAGGGCCTG CGGGGTCTAT 
     3301 TGGGAACCAA GCTGGAGTGC AGTGGCACAA TCTTGGCTCA CTGCAATCTC CGCCTCCTGG 
     3361 GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC GAGTTGTTGG GATTCCAGGC ATGCATGACC 
     3421 AGGCTCAGCT AATTTTTGTT TTTTTGGTAG AGACGGGGTT TCACCATATT GGCCAGGCTG 
     3481 GTCTCCAACT CCTAATCTCA GGTGATCTAC CCACCTTGGC CTCCCAAATT GCTGGGATTA 
     3541 CAGGCGTGAA CCACTGCTCC CTTCCCTGTC CTTCTGATTT TAAAATAACT ATACCAGCAG 
     3601 GAGGACGTCC AGACACAGCA TAGGCTACCT GGCCATGCCC AACCGGTGGG ACATTTGAGT 
     3661 TGCTTGCTTG GCACTGTCCT CTCATGCGTT GGGTCCACTC AGTAGATGCC TGTTGAATTG 
     3721 GGTACGCGGC CAGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC 
     3781 AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA 
     3841 CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA 
     3901 CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC 
     3961 GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA 
     4021 CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA 
     4081 TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA 
     4141 GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA 
     4201 CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG 
     4261 TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG 
     4321 TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG 
     4381 CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG 
     4441 AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA 
     4501 CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT 
     4561 CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AACCTGAGGC 
     4621 TATGGCAGGG CCTGCCGCCC CGACGTTGGC TGCGAGCCCT GGGCCTTCAC CCGAACTTGG 
     4681 GGGGTGGGGT GGGGAAAAGG AAGAAACGCG GGCGTATTGG CCCCAATGGG GTCTCGGTGG 
     4741 GGTATCGACA GAGTGCCAGC CCTGGGACCG AACCCCGCGT TTATGAACAA ACGACCCAAC 
     4801 ACCGTGCGTT TTATTCTGTC TTTTTATTGC CGTCATAGCG CGGGTTCCTT CCGGTATTGT 
     4861 CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT AGGGTGGGCG AAGAACTCCA GCATGAGATC 
     4921 CCCGCGCTGG AGGATCATCC AGCCGGCGTC CCGGAAAACG ATTCCGAAGC CCAACCTTTC 
     4981 ATAGAAGGCG GCGGTGGAAT CGAAATCTCG TGATGGCAGG TTGGGCGTCG CTTGGTCGGT 
     5041 CATTTCGAAC CCCAGAGTCC CGCTCAGAAG AACTCGTCAA GAAGGCGATA GAAGGCGATG 
     5101 CGCTGCGAAT CGGGAGCGGC GATACCGTAA AGCACGAGGA AGCGGTCAGC CCATTCGCCG 
     5161 CCAAGCTCTT CAGCAATATC ACGGGTAGCC AACGCTATGT CCTGATAGCG ATCCGCCACA 
     5221 CCCAGCCGGC CACAGTCGAT GAATCCAGAA AAGCGGCCAT TTTCCACCAT GATATTCGGC 
     5281 AAGCAGGCAT CGCCATGGGT CACGACGAGA TCCTCGCCGT CGGGCATGCT CGCCTTGAGC 
     5341 CTGGCGAACA GTTCGGCTGG CGCGAGCCCC TGATGCTCTT CGTCCAGATC ATCCTGATCG 
     5401 ACAAGACCGG CTTCCATCCG AGTACGTGCT CGCTCGATGC GATGTTTCGC TTGGTGGTCG 
     5461 AATGGGCAGG TAGCCGGATC AAGCGTATGC AGCCGCCGCA TTGCATCAGC CATGATGGAT 
     5521 ACTTTCTCGG CAGGAGCAAG GTGAGATGAC AGGAGATCCT GCCCCGGCAC TTCGCCCAAT 
     5581 AGCAGCCAGT CCCTTCCCGC TTCAGTGACA ACGTCGAGCA CAGCTGCGCA AGGAACGCCC 
     5641 GTCGTGGCCA GCCACGATAG CCGCGCTGCC TCGTCTTGCA GTTCATTCAG GGCACCGGAC 
     5701 AGGTCGGTCT TGACAAAAAG AACCGGGCGC CCCTGCGCTG ACAGCCGGAA CACGGCGGCA 
     5761 TCAGAGCAGC CGATTGTCTG TTGTGCCCAG TCATAGCCGA ATAGCCTCTC CACCCAAGCG 
     5821 GCCGGAGAAC CTGCGTGCAA TCCATCTTGT TCAATCATGC GAAACGATCC TCATCCTGTC 
     5881 TCTTGATCGA TCTTTGCAAA AGCCTAGGCC TCCAAAAAAG CCTCCTCACT ACTTCTGGAA 
     5941 TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC TGCATAAATA AAAAAAATTA GTCAGCCATG 
     6001 GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG 
     6061 GACTATGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     6121 TGGGGACTTT CCACACCTGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     6181 TGCTGGGGAG CCTGGGGACT TTCCACACCC TAACTGACAC ACATTCCACA GCTGGTTCTT 
     6241 TCCGCCTCAG GACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC 
     6301 ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA 
     6361 TTTCCCCGAA AAGTGCCACC TGACGCGCCC TGTAGCGGCG CATTAAGCGC GGCGGGTGTG 
     6421 GTGGTTACGC GCAGCGTGAC CGCTACACTT GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT 
     6481 TTCTTCCCTT CCTTTCTCGC CACGTTCGCC GGCTTTCCCC GTCAAGCTCT AAATCGGGGG 
     6541 CTCCCTTTAG GGTTCCGATT TAGTGCTTTA CGGCACCTCG ACCCCAAAAA ACTTGATTAG 
     6601 GGTGATGGTT CACGTAGTGG GCCATCGCCC TGATAGACGG TTTTTCGCCC TTTGACGTTG 
     6661 GAGTCCACGT TCTTTAATAG TGGACTCTTG TTCCAAACTG GAACAACACT CAACCCTATC 
     6721 TCGGTCTATT CTTTTGATTT ATAAGGGATT TTGCCGATTT CGGCCTATTG GTTAAAAAAT 
     6781 GAGCTGATTT AACAAAAATT TAACGCGAAT TTT
//