LOCUS       RR217019                6753 bp    DNA     circular  SYN 
DEFINITION  Parn (myc-DDK-tagged) - Rat poly(A)-specific ribonuclease (Parn)
ACCESSION   NM_001270415
VERSION     NM_001270415.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    2910..3001 
                     /label="Myc-DDK"  
     misc_feature    3031..3616 
                     /label="PolyA signal"  
     misc_feature    3088..3107 
                     /label="Restriction Enzyme"  
     misc_feature    3892..4564 
                     /label="ColE1"  
     misc_feature    4994..5788 
                     /label="Kan/Neo"  
     misc_feature    5823..6125 
                     /label="SV40 ori"  
     misc_feature    6211..6688 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    2892..2898 
                     /label="Restriction Enzyme"  
     misc_feature    1020..2892 
                     /label="Parn"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAGATAATC CGGAGCAATT TTAAGATTAA TCTTCCCAAA GTGTACCAGG 
     1081 CCATAGAGGA GGCTGACTTC TTCGCCATCG ATGGGGAGTT TTCAGGAATC AGTGATGGAC 
     1141 CCTCAGTAAC AGCATTAACA AGTGGTTTTG ACACCCCAGA AGAGAGATAT CAGAAGCTTA 
     1201 AAAAGCATTC CATGGACTTC TTGCTGTTTC AGTTTGGCCT CTGTGCTTTT AAGTATGACC 
     1261 ACGCCAATTC CAAGCATGTA ACGAAGTCGT TTAACTTCTA TGTTTTCCCC AAGCCTTTCA 
     1321 GTAGGTCCTC ACCAGATGTC AAGTTTGTTT GCCAGAGCTC CAGCATTGAC TTTCTGGCAA 
     1381 GCCAAGGATT TGACTTTAAT AAAGTGTTTT GCAGTGGAAT TCCATATCTG AACCAGGAAG 
     1441 AAGAAAGACA GCTGAGAGAG CAATTTGATG AAAAACGTTC TCAGGCCAAT GGGGCCGGAG 
     1501 CTCTGACAAA ATGTCCTGTG ACAATTCCTG AGGATCAGAA GAAGTTTATT GACCAAGTAA 
     1561 TAGAGAAGAT AGAGGGTTTC CTACAGAGAG AAGAGAAGAG GACTCTGGAA CTTAACCCAT 
     1621 GCACTGGGTT CCAAAGAAAA CTTATTTATC AGACTTTGAG TTGGAAGTAT CCCAAAGGCA 
     1681 TTCATGTTGA GACATTAGAG ACTGACAAGA AGGAAAGACA CATAGTTATC AGCAAAGTGG 
     1741 ATGAAGAAGA ACGCAAAAGG AGAGAACAGG AGAAGTACGC AAAGGAGCAG GAGGAACTGA 
     1801 ATGATGCTGT AGGATTTTCA AGAGTCATCC ATGCGATTGC TAATTCGGGG AAGCTGGTTG 
     1861 TTGGACACAA CATGCTCTTG GATGTCATGC ACACTATTCA CCAGTTCTAC TGCCCTCTGC 
     1921 CTGTGGACCT GAATGAGTTT AAGGAGATGA CAACATGTGT CTTCCCCAGA CTCTTGGATA 
     1981 CTAAATTGAT GGCCAGCACC CAGCCCTTTA AGGATATCAT TAACAATACA TCCCTTGCAG 
     2041 AGTTGGAAAA GCGGTTGAAA GAGACACCTT TTGACCCTCC TAAAGTTGAG AGTGCGGAAG 
     2101 GCTTTCCAAG CTACGACACA GCTTCTGAGC AGCTGCATGA GGCAGGTTAT GATGCGTACA 
     2161 TCACAGGACT CTGCTTCATC TCCATGGCAA ATTACTTAGG TTCTTTACTC AGTCCTCCAA 
     2221 AAACGTGTGT GTCCGCCAGA TCAAAGCTCA TCGAACCCTT TTTTAACAAG TTATTTCTTA 
     2281 TGAGGGTCAT GGATATTCCC TATTTAAACT TGGAAGGGCC AGACTTGCAG CCTAAGCGGG 
     2341 ATCATGTTCT CCATGTGACC TTCCCTAAAG AATGGAAAAC CAGTGACCTG TACCAGCTCT 
     2401 TCAGCGCCTT TGGTAACATT CAGATATCCT GGATTGATGA CACATCAGCC TTCGTTTCTC 
     2461 TCAGCCAACC AGAACAAGTA CAAATTGCTG TTAATACCAG TAAGTATGCT GAAAGTTATC 
     2521 GGATCCAGAC CTACGCTGAG TATGTGGGAA AGAAGCAGGA AAGCAAGCAG GTCAAGAGGA 
     2581 AGTGGACAGA AGACAGTTGG AAGGAGGTGG ACAGAAAGCG GCCCCACGTG CCGGGCCCCT 
     2641 GTTACCACAC CAACAGCTTC ACTGCAGCCG GCATAGTTGG AAAGAGAACT CTGAGTCCCG 
     2701 ACCCTCGGGA AGCTGGAGTG GTGGACAGAG AATCAGAGGG AATATCTGAC CCGGAACTTG 
     2761 AACAGACGGA TTACTGTGCA GACCCCCTCC CAGAGGGAAG GAAAAAGGCC AAGAAGTTAA 
     2821 AAAGAATGAA GAAAGAGTTT TCCTTGGCAG GAAGTGCCTC AGATAGCCCT GCTGTGCTTT 
     2881 TTGAAGTCCC CGACACGTGG ACGCGTACGC GGCCGCTCGA GCAGAAACTC ATCTCAGAAG 
     2941 AGGATCTGGC AGCAAATGAT ATCCTGGATT ACAAGGATGA CGACGATAAG GTTTAAACGG 
     3001 CCGGCCGCGG TCATAGCTGT TTCCTGAACA GATCCCGGGT GGCATCCCTG TGACCCCTCC 
     3061 CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC ACTCCAGTGC CCACCAGCCT TGTCCTAATA 
     3121 AAATTAAGTT GCATCATTTT GTCTGACTAG GTGTCCTTCT ATAATATTAT GGGGTGGAGG 
     3181 GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG AAGACAACCT GTAGGGCCTG CGGGGTCTAT 
     3241 TGGGAACCAA GCTGGAGTGC AGTGGCACAA TCTTGGCTCA CTGCAATCTC CGCCTCCTGG 
     3301 GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC GAGTTGTTGG GATTCCAGGC ATGCATGACC 
     3361 AGGCTCAGCT AATTTTTGTT TTTTTGGTAG AGACGGGGTT TCACCATATT GGCCAGGCTG 
     3421 GTCTCCAACT CCTAATCTCA GGTGATCTAC CCACCTTGGC CTCCCAAATT GCTGGGATTA 
     3481 CAGGCGTGAA CCACTGCTCC CTTCCCTGTC CTTCTGATTT TAAAATAACT ATACCAGCAG 
     3541 GAGGACGTCC AGACACAGCA TAGGCTACCT GGCCATGCCC AACCGGTGGG ACATTTGAGT 
     3601 TGCTTGCTTG GCACTGTCCT CTCATGCGTT GGGTCCACTC AGTAGATGCC TGTTGAATTG 
     3661 GGTACGCGGC CAGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC 
     3721 AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA 
     3781 CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA 
     3841 CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC 
     3901 GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA 
     3961 CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA 
     4021 TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA 
     4081 GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA 
     4141 CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG 
     4201 TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG 
     4261 TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG 
     4321 CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG 
     4381 AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA 
     4441 CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT 
     4501 CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AACCTGAGGC 
     4561 TATGGCAGGG CCTGCCGCCC CGACGTTGGC TGCGAGCCCT GGGCCTTCAC CCGAACTTGG 
     4621 GGGGTGGGGT GGGGAAAAGG AAGAAACGCG GGCGTATTGG CCCCAATGGG GTCTCGGTGG 
     4681 GGTATCGACA GAGTGCCAGC CCTGGGACCG AACCCCGCGT TTATGAACAA ACGACCCAAC 
     4741 ACCGTGCGTT TTATTCTGTC TTTTTATTGC CGTCATAGCG CGGGTTCCTT CCGGTATTGT 
     4801 CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT AGGGTGGGCG AAGAACTCCA GCATGAGATC 
     4861 CCCGCGCTGG AGGATCATCC AGCCGGCGTC CCGGAAAACG ATTCCGAAGC CCAACCTTTC 
     4921 ATAGAAGGCG GCGGTGGAAT CGAAATCTCG TGATGGCAGG TTGGGCGTCG CTTGGTCGGT 
     4981 CATTTCGAAC CCCAGAGTCC CGCTCAGAAG AACTCGTCAA GAAGGCGATA GAAGGCGATG 
     5041 CGCTGCGAAT CGGGAGCGGC GATACCGTAA AGCACGAGGA AGCGGTCAGC CCATTCGCCG 
     5101 CCAAGCTCTT CAGCAATATC ACGGGTAGCC AACGCTATGT CCTGATAGCG ATCCGCCACA 
     5161 CCCAGCCGGC CACAGTCGAT GAATCCAGAA AAGCGGCCAT TTTCCACCAT GATATTCGGC 
     5221 AAGCAGGCAT CGCCATGGGT CACGACGAGA TCCTCGCCGT CGGGCATGCT CGCCTTGAGC 
     5281 CTGGCGAACA GTTCGGCTGG CGCGAGCCCC TGATGCTCTT CGTCCAGATC ATCCTGATCG 
     5341 ACAAGACCGG CTTCCATCCG AGTACGTGCT CGCTCGATGC GATGTTTCGC TTGGTGGTCG 
     5401 AATGGGCAGG TAGCCGGATC AAGCGTATGC AGCCGCCGCA TTGCATCAGC CATGATGGAT 
     5461 ACTTTCTCGG CAGGAGCAAG GTGAGATGAC AGGAGATCCT GCCCCGGCAC TTCGCCCAAT 
     5521 AGCAGCCAGT CCCTTCCCGC TTCAGTGACA ACGTCGAGCA CAGCTGCGCA AGGAACGCCC 
     5581 GTCGTGGCCA GCCACGATAG CCGCGCTGCC TCGTCTTGCA GTTCATTCAG GGCACCGGAC 
     5641 AGGTCGGTCT TGACAAAAAG AACCGGGCGC CCCTGCGCTG ACAGCCGGAA CACGGCGGCA 
     5701 TCAGAGCAGC CGATTGTCTG TTGTGCCCAG TCATAGCCGA ATAGCCTCTC CACCCAAGCG 
     5761 GCCGGAGAAC CTGCGTGCAA TCCATCTTGT TCAATCATGC GAAACGATCC TCATCCTGTC 
     5821 TCTTGATCGA TCTTTGCAAA AGCCTAGGCC TCCAAAAAAG CCTCCTCACT ACTTCTGGAA 
     5881 TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC TGCATAAATA AAAAAAATTA GTCAGCCATG 
     5941 GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG 
     6001 GACTATGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     6061 TGGGGACTTT CCACACCTGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     6121 TGCTGGGGAG CCTGGGGACT TTCCACACCC TAACTGACAC ACATTCCACA GCTGGTTCTT 
     6181 TCCGCCTCAG GACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC 
     6241 ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA 
     6301 TTTCCCCGAA AAGTGCCACC TGACGCGCCC TGTAGCGGCG CATTAAGCGC GGCGGGTGTG 
     6361 GTGGTTACGC GCAGCGTGAC CGCTACACTT GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT 
     6421 TTCTTCCCTT CCTTTCTCGC CACGTTCGCC GGCTTTCCCC GTCAAGCTCT AAATCGGGGG 
     6481 CTCCCTTTAG GGTTCCGATT TAGTGCTTTA CGGCACCTCG ACCCCAAAAA ACTTGATTAG 
     6541 GGTGATGGTT CACGTAGTGG GCCATCGCCC TGATAGACGG TTTTTCGCCC TTTGACGTTG 
     6601 GAGTCCACGT TCTTTAATAG TGGACTCTTG TTCCAAACTG GAACAACACT CAACCCTATC 
     6661 TCGGTCTATT CTTTTGATTT ATAAGGGATT TTGCCGATTT CGGCCTATTG GTTAAAAAAT 
     6721 GAGCTGATTT AACAAAAATT TAACGCGAAT TTT
//