LOCUS       RR216916                6564 bp    DNA     circular  SYN 
DEFINITION  Ces2 (myc-DDK-tagged) - Rat carboxylesterase 2 (Ces2)
ACCESSION   NM_001271303
VERSION     NM_001271303.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    2721..2812 
                     /label="Myc-DDK"  
     misc_feature    2842..3427 
                     /label="PolyA signal"  
     misc_feature    2899..2918 
                     /label="Restriction Enzyme"  
     misc_feature    3703..4375 
                     /label="ColE1"  
     misc_feature    4805..5599 
                     /label="Kan/Neo"  
     misc_feature    5634..5936 
                     /label="SV40 ori"  
     misc_feature    6022..6499 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    2703..2709 
                     /label="Restriction Enzyme"  
     misc_feature    1020..2703 
                     /label="Ces2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCCACGGAAC CAACTGCATA GCTGGCTGAA TGCTGTGCTC TTTGGGCTGC 
     1081 TGCTTCTTCT TATTCATGTG CAGGGTCAGG ACTCACCGGA GTCCAGCCCC ATCAGGACCA 
     1141 CACACACTGG CCAGGTCCAA GGCAAGCTCG ACCACGTGAA GGACACTAAA GCTGGTGTCC 
     1201 ACACCTTCCT GGGAATTCCC TTTGCCAAGC CTCCTGTAGG ACCGCTGCGC TTTGCACCTC 
     1261 CTGAGCCACC TGAGCCATGG AGTGGTGTGA GAGATGCGAC CTCACAGCCG GCCATGTGTC 
     1321 TGCAAAATCT TGATATTCTG GATGAAGTAG GCCTGCTGGA TATGAAAATG ATACTGTCTT 
     1381 CCATTTCTAT GTCTGAGGAC TGCCTGTATC TCAATGTCTA TGCACCAGCC CATGCCCGTG 
     1441 AGGGCTCTAA CCTGCCTGTG ATGGTGTGGA TCCACGGAGG TGCGCTGGTT GTAGGAATGG 
     1501 CTTCCATGTA TGATGGATCT CTATTGACAG TCAATGAGGA CTTGGTGGTT GTCACTATTC 
     1561 AGTATCGTCT GGGTGTCCTG GGCTTTTTCA GCACTGGAGA TGAGCACGCC AGAGGCAACT 
     1621 GGGGATACCT GGACCAAGTG GCTGCCCTAC GTTGGGTCCA GCAGAATATC GCCCATTTTG 
     1681 GAGGCAACCC TAACCGTGTC ACTATCTTTG GCGAGTCTGC AGGTGGCACA AGTGTGTCTT 
     1741 CACATGTTAT ATCCCCCATG TCTCAAGGGC TCTTCCATGG TGCCATCATG GAGAGTGGAG 
     1801 TGGCCCTGCT GCCTGACCTT ATCTCTGAAA CCTCTGAAAC GGTCTCCACT ACAGTGGCCA 
     1861 AGCTCTCTGG ATGTGAGGCT ATGGACTCAG AGGCCCTGGT GCGCTGCCTG AGAGCGAAGA 
     1921 GTGGAGCAGA GATTCTGGTC ATTAACAAGG TCTTCAAGAT GATCCCCGCT GTGGTGGATG 
     1981 GAGAGTTCCT ACCCAGGCAT CCCAAAGAGC TGTTGGCATC TGAGGATTTT CACCCTGTCC 
     2041 CCAGCATCAT TGGTGTTAAC ACAGATGAGT ACTGTTGCAC CATTCCTATG GTCATGGGCA 
     2101 CTGCTCAAAT AATAAAGGAG CTATCCAGAG AGAACCTGCA GGCTGTTCTA AAGGATACAG 
     2161 CAGCACAAAT GATGCTTCCT CCTGAGTGTG GTGACCTGCT CATGGAAGAG TACATGGGGA 
     2221 ACACTGATGA TTCCCAGACC CTACAAATAC AGTACACAGA GATGATGGGA GACTTCCTGT 
     2281 TTGTGATCCC TGCACTCCAA GTAGCACACT TTCAGCGTTC CCATGCCCCT GTCTACTTCT 
     2341 ATGAGTTCCA ACATGCACCC AGCTATTTCA AGAATGTCAG GCCACCCCAC GTGAAGGCTG 
     2401 ACCATGCTGA TGAGGTTCCT TTTGTCTTTG GGTCCTTCTT TTCGGGCATG AAACTTGACT 
     2461 TCACTGAGGA GGAGAGGCTG CTGAGCAGGA GGATGATGAA GTACTGGGCC AACTTTGCAA 
     2521 GACAGGGGAA CCCCAACAGC GAGGGTCTAC CCTACTGGCC TGCATTGGAC CACGATGAGC 
     2581 AGTACCTGCA GTTGGACACC CATCCTGCTG TGGACCGAGC CCTGAAGGCC AGAAGGCTGC 
     2641 AGTTCTGGAC CAAGACTCTG CCCCAGAAGA TTGAGGAGCT AAATGGAGCT CAGAAAAACC 
     2701 ATGCAGAGCT GACGCGTACG CGGCCGCTCG AGCAGAAACT CATCTCAGAA GAGGATCTGG 
     2761 CAGCAAATGA TATCCTGGAT TACAAGGATG ACGACGATAA GGTTTAAACG GCCGGCCGCG 
     2821 GTCATAGCTG TTTCCTGAAC AGATCCCGGG TGGCATCCCT GTGACCCCTC CCCAGTGCCT 
     2881 CTCCTGGCCC TGGAAGTTGC CACTCCAGTG CCCACCAGCC TTGTCCTAAT AAAATTAAGT 
     2941 TGCATCATTT TGTCTGACTA GGTGTCCTTC TATAATATTA TGGGGTGGAG GGGGGTGGTA 
     3001 TGGAGCAAGG GGCAAGTTGG GAAGACAACC TGTAGGGCCT GCGGGGTCTA TTGGGAACCA 
     3061 AGCTGGAGTG CAGTGGCACA ATCTTGGCTC ACTGCAATCT CCGCCTCCTG GGTTCAAGCG 
     3121 ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG GGATTCCAGG CATGCATGAC CAGGCTCAGC 
     3181 TAATTTTTGT TTTTTTGGTA GAGACGGGGT TTCACCATAT TGGCCAGGCT GGTCTCCAAC 
     3241 TCCTAATCTC AGGTGATCTA CCCACCTTGG CCTCCCAAAT TGCTGGGATT ACAGGCGTGA 
     3301 ACCACTGCTC CCTTCCCTGT CCTTCTGATT TTAAAATAAC TATACCAGCA GGAGGACGTC 
     3361 CAGACACAGC ATAGGCTACC TGGCCATGCC CAACCGGTGG GACATTTGAG TTGCTTGCTT 
     3421 GGCACTGTCC TCTCATGCGT TGGGTCCACT CAGTAGATGC CTGTTGAATT GGGTACGCGG 
     3481 CCAGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG 
     3541 GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA 
     3601 GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG 
     3661 ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC 
     3721 TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC 
     3781 CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC 
     3841 GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG 
     3901 CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC 
     3961 ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA 
     4021 GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC 
     4081 TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC 
     4141 CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG 
     4201 ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC 
     4261 ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA 
     4321 TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAACCTGAGG CTATGGCAGG 
     4381 GCCTGCCGCC CCGACGTTGG CTGCGAGCCC TGGGCCTTCA CCCGAACTTG GGGGGTGGGG 
     4441 TGGGGAAAAG GAAGAAACGC GGGCGTATTG GCCCCAATGG GGTCTCGGTG GGGTATCGAC 
     4501 AGAGTGCCAG CCCTGGGACC GAACCCCGCG TTTATGAACA AACGACCCAA CACCGTGCGT 
     4561 TTTATTCTGT CTTTTTATTG CCGTCATAGC GCGGGTTCCT TCCGGTATTG TCTCCTTCCG 
     4621 TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC GAAGAACTCC AGCATGAGAT CCCCGCGCTG 
     4681 GAGGATCATC CAGCCGGCGT CCCGGAAAAC GATTCCGAAG CCCAACCTTT CATAGAAGGC 
     4741 GGCGGTGGAA TCGAAATCTC GTGATGGCAG GTTGGGCGTC GCTTGGTCGG TCATTTCGAA 
     4801 CCCCAGAGTC CCGCTCAGAA GAACTCGTCA AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA 
     4861 TCGGGAGCGG CGATACCGTA AAGCACGAGG AAGCGGTCAG CCCATTCGCC GCCAAGCTCT 
     4921 TCAGCAATAT CACGGGTAGC CAACGCTATG TCCTGATAGC GATCCGCCAC ACCCAGCCGG 
     4981 CCACAGTCGA TGAATCCAGA AAAGCGGCCA TTTTCCACCA TGATATTCGG CAAGCAGGCA 
     5041 TCGCCATGGG TCACGACGAG ATCCTCGCCG TCGGGCATGC TCGCCTTGAG CCTGGCGAAC 
     5101 AGTTCGGCTG GCGCGAGCCC CTGATGCTCT TCGTCCAGAT CATCCTGATC GACAAGACCG 
     5161 GCTTCCATCC GAGTACGTGC TCGCTCGATG CGATGTTTCG CTTGGTGGTC GAATGGGCAG 
     5221 GTAGCCGGAT CAAGCGTATG CAGCCGCCGC ATTGCATCAG CCATGATGGA TACTTTCTCG 
     5281 GCAGGAGCAA GGTGAGATGA CAGGAGATCC TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG 
     5341 TCCCTTCCCG CTTCAGTGAC AACGTCGAGC ACAGCTGCGC AAGGAACGCC CGTCGTGGCC 
     5401 AGCCACGATA GCCGCGCTGC CTCGTCTTGC AGTTCATTCA GGGCACCGGA CAGGTCGGTC 
     5461 TTGACAAAAA GAACCGGGCG CCCCTGCGCT GACAGCCGGA ACACGGCGGC ATCAGAGCAG 
     5521 CCGATTGTCT GTTGTGCCCA GTCATAGCCG AATAGCCTCT CCACCCAAGC GGCCGGAGAA 
     5581 CCTGCGTGCA ATCCATCTTG TTCAATCATG CGAAACGATC CTCATCCTGT CTCTTGATCG 
     5641 ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA GCCTCCTCAC TACTTCTGGA ATAGCTCAGA 
     5701 GGCCGAGGCG GCCTCGGCCT CTGCATAAAT AAAAAAAATT AGTCAGCCAT GGGGCGGAGA 
     5761 ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG GATGGGCGGA GTTAGGGGCG GGACTATGGT 
     5821 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     5881 TCCACACCTG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     5941 GCCTGGGGAC TTTCCACACC CTAACTGACA CACATTCCAC AGCTGGTTCT TTCCGCCTCA 
     6001 GGACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA 
     6061 TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA 
     6121 AAAGTGCCAC CTGACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT GGTGGTTACG 
     6181 CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT 
     6241 TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC TAAATCGGGG GCTCCCTTTA 
     6301 GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA AACTTGATTA GGGTGATGGT 
     6361 TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT GGAGTCCACG 
     6421 TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACCCTAT CTCGGTCTAT 
     6481 TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT GGTTAAAAAA TGAGCTGATT 
     6541 TAACAAAAAT TTAACGCGAA TTTT
//