LOCUS       RR215669                6789 bp    DNA     circular  SYN 
DEFINITION  Gspt1 (Myc-DDK-tagged ORF) - Rat G1 to S phase transition 1 (Gspt1), (10 ug)
ACCESSION   NM_001003978
VERSION     NM_001003978.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    2946..3037 
                     /label="Myc-DDK"  
     misc_feature    3067..3652 
                     /label="PolyA signal"  
     misc_feature    3124..3143 
                     /label="Restriction Enzyme"  
     misc_feature    3928..4600 
                     /label="ColE1"  
     misc_feature    5030..5824 
                     /label="Kan/Neo"  
     misc_feature    5859..6161 
                     /label="SV40 ori"  
     misc_feature    6247..6724 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    2928..2934 
                     /label="Restriction Enzyme"  
     misc_feature    1020..2928 
                     /label="Gspt1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGATCCCAGC AGCGGAGGTG GTGGCGGAGG CGGCGGCGGC GGCGGCGGGA 
     1081 GCAGCAGCAG CAGTAGCGAC TCTGCGCCCG ACTGCTGGGA CCAGACCGAT ATGGAGGCTC 
     1141 CCGGGCCGGG GCCTTGCGGC GGTGGCTCCA TGGCCGCGGT GGCCGAGGCC CAGCGTGAGA 
     1201 ACCTCAGCGC GGCTTTCAGC CGGCAACTCA ACGTCAACGC CAAGCCCTTC GTCCCCAACG 
     1261 TCCACGCCGC GGAATTCGTG CCGTCTTTCC TGCGAGGTCC GGCCCAGCCG CCGCTATCCC 
     1321 CGGCTGGCGC GGCCGGCGGT GACCACGGAG CGGGCAGCAG CGCGGGAGGC CCTTCGGAAC 
     1381 CTGTGGAGTC CTCTCAAGAG CAGTCATTGT GTGAAGGTTC AAATTCTACT GTTAGCATGG 
     1441 AACTTTCAGA ACCTGTTGTA GAAAATGGAG AGACAGAAAT GTCCCCAGAA GAATCATGGG 
     1501 AGCACAAAGA AGAAATAAGT GAAGCCGAGC CAGCAGGTGG TGGTTCCTCA GGAGATGGAA 
     1561 GGCCACCAGA GGAAAGCACC CAAGAAATGA TGGAGGAGGA AGAAGAAATA CCAAAACCTA 
     1621 AGTCAGTGGT GGCACCACCA GGCGCTCCTA AGAAAGAGCA TGTAAATGTA GTGTTCATCG 
     1681 GGCATGTGGA TGCTGGCAAG TCAACCATTG GAGGACAAAT AATGTATTTG ACTGGAATGG 
     1741 TCGACAAAAG AACACTTGAG AAATATGAAC GAGAAGCTAA AGAAAAAAAC AGAGAAACTT 
     1801 GGTACTTGTC TTGGGCCTTA GACACAAATC AGGAAGAGCG AGACAAGGGT AAAACAGTAG 
     1861 AGGTGGGCCG TGCCTATTTT GAAACGGAAA AAAAGCATTT TACAATCCTA GATGCTCCTG 
     1921 GCCATAAGAG TTTTGTCCCA AATATGATTG GTGGTGCCTC TCAAGCTGAC TTGGCTGTAC 
     1981 TGGTAATCTC AGCCCGGAAA GGAGAGTTTG AAACTGGATT TGAAAAAGGA GGACAGACAA 
     2041 GAGAACATGC GATGTTGGCA AAGACAGCAG GTGTCAAACA CTTAATCGTG CTTATTAATA 
     2101 AGATGGATGA TCCAACAGTA AACTGGAGCA ATGAGAGATA TGAAGAATGC AAAGAGAAAT 
     2161 TAATACCATT TTTAAAGAAA GTTGGCTTCA ATCCCAAAAA GGACATTCAT TTTATGCCCT 
     2221 GCTCAGGACT GACAGGAGCA AATCTTAAAG AGCAATCAGA TTTCTGTCCT TGGTACATTG 
     2281 GCTTACCATT TATTCCATAT CTGGATAATT TGCCAAACTT CAATAGATCA GTTGATGGAC 
     2341 CAATCAGGCT GCCAATTGTG GATAAGTACA AGGATATGGG GACTGTGGTC CTGGGAAAGC 
     2401 TGGAATCTGG ATCCATCTGT AAAGGCCAGC AGCTCGTAAT GATGCCCAAT AAGCACAACG 
     2461 TGGAGGTCCT TGGAATACTT TCTGATGATG TAGAGACGGA CTCTGTAGCT CCAGGTGAAA 
     2521 ACCTCAAAAT CAGACTGAAA GGGATTGAAG AGGAAGAGAT TCTTCCAGGA TTCATCCTCT 
     2581 GTGATCTTAA TAATCTTTGT CATTCTGGAC GCACATTCGA TGCCCAGATA GTGATTATAG 
     2641 AGCACAAGTC CATCATCTGC CCAGGGTATA ATGCGGTGCT GCACATTCAC ACCTGTATCG 
     2701 AGGAAGTCGA GATAACGGCC TTAATCTGCT TGGTAGACAA AAAATCAGGA GAAAAAAGTA 
     2761 AGACTCGACC CCGTTTTGTA AAGCAAGATC AAGTATGCAT TGCTCGTTTA AGGACAGCAG 
     2821 GAACCATCTG CCTGGAGACC TTTAAGGACT TCCCTCAGAT GGGACGTTTT ACCTTAAGAG 
     2881 ATGAAGGTAA GACCATTGCA ATTGGAAAAG TTCTGAAACT GGTTCCAGAG AAAGACACGC 
     2941 GTACGCGGCC GCTCGAGCAG AAACTCATCT CAGAAGAGGA TCTGGCAGCA AATGATATCC 
     3001 TGGATTACAA GGATGACGAC GATAAGGTTT AAACGGCCGG CCGCGGTCAT AGCTGTTTCC 
     3061 TGAACAGATC CCGGGTGGCA TCCCTGTGAC CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA 
     3121 GTTGCCACTC CAGTGCCCAC CAGCCTTGTC CTAATAAAAT TAAGTTGCAT CATTTTGTCT 
     3181 GACTAGGTGT CCTTCTATAA TATTATGGGG TGGAGGGGGG TGGTATGGAG CAAGGGGCAA 
     3241 GTTGGGAAGA CAACCTGTAG GGCCTGCGGG GTCTATTGGG AACCAAGCTG GAGTGCAGTG 
     3301 GCACAATCTT GGCTCACTGC AATCTCCGCC TCCTGGGTTC AAGCGATTCT CCTGCCTCAG 
     3361 CCTCCCGAGT TGTTGGGATT CCAGGCATGC ATGACCAGGC TCAGCTAATT TTTGTTTTTT 
     3421 TGGTAGAGAC GGGGTTTCAC CATATTGGCC AGGCTGGTCT CCAACTCCTA ATCTCAGGTG 
     3481 ATCTACCCAC CTTGGCCTCC CAAATTGCTG GGATTACAGG CGTGAACCAC TGCTCCCTTC 
     3541 CCTGTCCTTC TGATTTTAAA ATAACTATAC CAGCAGGAGG ACGTCCAGAC ACAGCATAGG 
     3601 CTACCTGGCC ATGCCCAACC GGTGGGACAT TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA 
     3661 TGCGTTGGGT CCACTCAGTA GATGCCTGTT GAATTGGGTA CGCGGCCAGC GGCGAGCGGT 
     3721 ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA 
     3781 GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC 
     3841 GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG 
     3901 GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT 
     3961 GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG 
     4021 AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG 
     4081 CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG 
     4141 TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC 
     4201 TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG 
     4261 GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT 
     4321 TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG 
     4381 TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC 
     4441 TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT 
     4501 GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT 
     4561 TAAATCAATC TAAAGTATAT ATGAGTAACC TGAGGCTATG GCAGGGCCTG CCGCCCCGAC 
     4621 GTTGGCTGCG AGCCCTGGGC CTTCACCCGA ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA 
     4681 AACGCGGGCG TATTGGCCCC AATGGGGTCT CGGTGGGGTA TCGACAGAGT GCCAGCCCTG 
     4741 GGACCGAACC CCGCGTTTAT GAACAAACGA CCCAACACCG TGCGTTTTAT TCTGTCTTTT 
     4801 TATTGCCGTC ATAGCGCGGG TTCCTTCCGG TATTGTCTCC TTCCGTGTTT CAGTTAGCCT 
     4861 CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT GAGATCCCCG CGCTGGAGGA TCATCCAGCC 
     4921 GGCGTCCCGG AAAACGATTC CGAAGCCCAA CCTTTCATAG AAGGCGGCGG TGGAATCGAA 
     4981 ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG GTCGGTCATT TCGAACCCCA GAGTCCCGCT 
     5041 CAGAAGAACT CGTCAAGAAG GCGATAGAAG GCGATGCGCT GCGAATCGGG AGCGGCGATA 
     5101 CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT TCGCCGCCAA GCTCTTCAGC AATATCACGG 
     5161 GTAGCCAACG CTATGTCCTG ATAGCGATCC GCCACACCCA GCCGGCCACA GTCGATGAAT 
     5221 CCAGAAAAGC GGCCATTTTC CACCATGATA TTCGGCAAGC AGGCATCGCC ATGGGTCACG 
     5281 ACGAGATCCT CGCCGTCGGG CATGCTCGCC TTGAGCCTGG CGAACAGTTC GGCTGGCGCG 
     5341 AGCCCCTGAT GCTCTTCGTC CAGATCATCC TGATCGACAA GACCGGCTTC CATCCGAGTA 
     5401 CGTGCTCGCT CGATGCGATG TTTCGCTTGG TGGTCGAATG GGCAGGTAGC CGGATCAAGC 
     5461 GTATGCAGCC GCCGCATTGC ATCAGCCATG ATGGATACTT TCTCGGCAGG AGCAAGGTGA 
     5521 GATGACAGGA GATCCTGCCC CGGCACTTCG CCCAATAGCA GCCAGTCCCT TCCCGCTTCA 
     5581 GTGACAACGT CGAGCACAGC TGCGCAAGGA ACGCCCGTCG TGGCCAGCCA CGATAGCCGC 
     5641 GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA CCGGACAGGT CGGTCTTGAC AAAAAGAACC 
     5701 GGGCGCCCCT GCGCTGACAG CCGGAACACG GCGGCATCAG AGCAGCCGAT TGTCTGTTGT 
     5761 GCCCAGTCAT AGCCGAATAG CCTCTCCACC CAAGCGGCCG GAGAACCTGC GTGCAATCCA 
     5821 TCTTGTTCAA TCATGCGAAA CGATCCTCAT CCTGTCTCTT GATCGATCTT TGCAAAAGCC 
     5881 TAGGCCTCCA AAAAAGCCTC CTCACTACTT CTGGAATAGC TCAGAGGCCG AGGCGGCCTC 
     5941 GGCCTCTGCA TAAATAAAAA AAATTAGTCA GCCATGGGGC GGAGAATGGG CGGAACTGGG 
     6001 CGGAGTTAGG GGCGGGATGG GCGGAGTTAG GGGCGGGACT ATGGTTGCTG ACTAATTGAG 
     6061 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCTGGTTGC 
     6121 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     6181 ACACCCTAAC TGACACACAT TCCACAGCTG GTTCTTTCCG CCTCAGGACT CTTCCTTTTT 
     6241 CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT 
     6301 ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC 
     6361 GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG TTACGCGCAG CGTGACCGCT 
     6421 ACACTTGCCA GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT TCTCGCCACG 
     6481 TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC CTTTAGGGTT CCGATTTAGT 
     6541 GCTTTACGGC ACCTCGACCC CAAAAAACTT GATTAGGGTG ATGGTTCACG TAGTGGGCCA 
     6601 TCGCCCTGAT AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA 
     6661 CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG TCTATTCTTT TGATTTATAA 
     6721 GGGATTTTGC CGATTTCGGC CTATTGGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC 
     6781 GCGAATTTT
//