LOCUS       RR215521                8364 bp    DNA     circular  SYN 
DEFINITION  Itgad (Myc-DDK-tagged ORF) - Rat integrin, alpha D (Itgad), (10 ug)
ACCESSION   NM_031691
VERSION     NM_031691.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4521..4612 
                     /label="Myc-DDK"  
     misc_feature    4642..5227 
                     /label="PolyA signal"  
     misc_feature    4699..4718 
                     /label="Restriction Enzyme"  
     misc_feature    5503..6175 
                     /label="ColE1"  
     misc_feature    6605..7399 
                     /label="Kan/Neo"  
     misc_feature    7434..7736 
                     /label="SV40 ori"  
     misc_feature    7822..8299 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4503..4509 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4503 
                     /label="Itgad"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCCGGTGGA GTTGTGATCC TCCTGTGTGG CTGGGTCCTG GCTTCCTGTC 
     1081 ATGGGTCTAA CCTGGATGTG GAGGAACCCA TCGTGTTCAG AGAGGATGCA GCCAGCTTTG 
     1141 GACAGACTGT GGTGCAGTTT GGTGGATCTC GACTCGTGGT GGGAGCCCCT CTGGAGGCGG 
     1201 TGGCAGTCAA CCAAACAGGA CGGTTGTATG ACTGTGCACC TGCCACTGGC ATGTGCCAGC 
     1261 CCATCGTACT GCGCAGTCCC CTAGAGGCAG TGAACATGTC CCTGGGCCTG TCTCTGGTGA 
     1321 CTGCCACCAA TAACGCCCAG TTGCTGGCTT GTGGTCCAAC TGCACAGAGA GCTTGTGTGA 
     1381 AGAACATGTA TGCGAAAGGT TCCTGCCTCC TTCTCGGCTC CAGCTTGCAG TTCATCCAGG 
     1441 CAGTCCCTGC CTCCATGCCA GAGTGTCCAA GACAAGAGAT GGACATTGCT TTCCTGATTG 
     1501 ATGGTTCTGG CAGCATTAAC CAAAGGGACT TTGCCCAGAT GAAGGACTTT GTCAAAGCTT 
     1561 TGATGGGAGA GTTTGCGAGC ACCAGCACCT TGTTCTCCCT GATGCAATAC TCGAACATCC 
     1621 TGAAGACCCA TTTTACCTTC ACTGAATTCA AGAACATCCT GGACCCTCAG AGCCTGGTGG 
     1681 ATCCCATTGT CCAGCTGCAA GGCCTGACCT ACACAGCCAC AGGCATCCGG ACAGTGATGG 
     1741 AAGAGCTATT TCATAGCAAG AATGGGTCCC GTAAAAGTGC CAAGAAGATC CTCCTTGTCA 
     1801 TCACAGATGG GCAGAAATAC AGAGACCCCC TGGAGTATAG TGATGTCATT CCCGCCGCAG 
     1861 ACAAAGCTGG CATCATTCGT TATGCTATTG GGGTGGGAGA TGCCTTCCAG GAGCCCACTG 
     1921 CCCTGAAGGA GCTGAACACC ATTGGCTCAG CTCCCCCACA GGACCACGTG TTCAAGGTAG 
     1981 GCAACTTTGC AGCACTTCGC AGCATCCAGA GGCAACTTCA GGAGAAAATC TTCGCCATTG 
     2041 AGGGAACTCA ATCAAGGTCA AGTAGTTCCT TTCAGCACGA GATGTCACAA GAAGGTTTCA 
     2101 GTTCAGCTCT CACATCGGAT GGACCCGTTC TGGGGGCCGT GGGAAGCTTC AGCTGGTCCG 
     2161 GAGGTGCCTT CTTATATCCC CCAAATACGA GACCCACCTT TATCAACATG TCTCAGGAGA 
     2221 ATGTGGACAT GAGAGACTCC TACCTGGGTT ACTCCACCGC AGTGGCCTTT TGGAAGGGGG 
     2281 TTCACAGCCT GATCCTGGGG GCCCCGCGTC ACCAGCACAC GGGGAAGGTT GTCATCTTTA 
     2341 CCCAGGAAGC CAGGCATTGG AGGCCCAAGT CTGAAGTCAG AGGGACACAG ATCGGCTCCT 
     2401 ACTTCGGGGC CTCTCTCTGT TCTGTGGACG TGGATAGAGA TGGCAGCACY GACCTGGTCC 
     2461 TGATCGGAGC CCCCCATTAC TATGAGCAGA CCCGAGGGGG GCAGGTCTCA GTGTTCCCCG 
     2521 TGCCCGGTGT GAGGGGCAGG TGGCAGTGTG AGGCCACCCT CCACGGGGAG CAGGGCCATC 
     2581 CTTGGGGCCG CTTTGGGGTG GCTCTGACAG TGCTGGGGGA CGTAAACGGG GACAATCTGG 
     2641 CAGACGTGGC TATTGGTGCC CCTGGAGAGG AGGAGAGCAG AGGTGCTGTC TACATATTTC 
     2701 ATGGAGCCTC GAGACTGGAG ATCATGCCCT CACCCAGCCA GCGGGTCACT GGCTCCCAGC 
     2761 TCTCCCTGAG ACTGCAGTAT TTTGGGCAGT CATTGAGTGG GGGTCAGGAC CTTACACAGG 
     2821 ATGGCCTGGT GGACCTGGCC GTGGGAGCCC AGGGGCACGT ACTGCTGCTC AGGAGTCTGC 
     2881 CTCTGCTGAA AGTGGAGCTC TCCATAAGAT TCGCCCCCAT GGAGGTGGCA AAGGCTGTGT 
     2941 ACCAGTGCTG GGAAAGGACT CCCACTGTCC TCGAAGCTGG AGAGGCCACT GTCTGTCTCA 
     3001 CTGTCCACAA AGGCTCACCT GACCTGTTAG GTAATGTCCA AGGCTCTGTC AGGTATGATC 
     3061 TGGCGTTAGA TCCGGGCCGC CTGATTTCTC GTGCCATTTT TGATGAGACT AAGAACTGCA 
     3121 CTTTGACGGG AAGGAAGACT CTGGGGCTTG GTGATCACTG CGAAACAGTG AAGCTGCTTT 
     3181 TGCCGGACTG TGTGGAAGAT GCAGTGAGCC CTATCATCCT GCGCCTCAAC TTTTCCCTGG 
     3241 TGAGAGACTC TGCTTCACCC AGGAACCTGC ATCCTGTGCT GGCTGTGGGC TCACAAGACC 
     3301 ACATAACTGC TTCTCTGCCG TTTGAGAAGA ACTGTAAGCA AGAACTCCTG TGTGAGGGGG 
     3361 ACCTGGGCAT CAGCTTTAAC TTCTCAGGCC TGCAGGTCTT GGTGGTGGGA GGCTCCCCAG 
     3421 AGCTCACTGT GACAGTCACT GTGTGGAATG AGGGTGAGGA CAGCTATGGA ACTTTAGTCA 
     3481 AGTTCTACTA CCCAGCAGGG CTATCTTACC GACGGGTAAC AGGGACTCAG CAACCTCATC 
     3541 AGTACCCACT ACGCTTGGCC TGTGAGGCTG AGCCCGCTGC CCAGGAGGAC CTGAGGAGCA 
     3601 GCAGCTGTAG CATTAATCAC CCCATCTTCC GAGAAGGTGC AAAGACCACC TTCATGATCA 
     3661 CATTCGATGT CTCCTACAAG GCCTTCCTAG GAGACAGGTT GCTTCTGAGG GCCAAAGCCA 
     3721 GCAGTGAGAA TAATAAGCCT GATACCAACA AGACTGCCTT CCAGCTGGAG CTCCCAGTGA 
     3781 AGTACACCGT CTATACCCTG ATCAGTAGGC AAGAAGATTC CACCAACCAT GTCAACTTTT 
     3841 CATCTTCCCA CGGGGGGAGA AGGCAAGAAG CCGCACATCG CTATCGTGTG AATAACCTGA 
     3901 GTCCACTGAA GCTGGCCGTC AGAGTTAACT TCTGGGTCCC TGTCCTTCTG AACGGTGTGG 
     3961 CTGTGTGGGA CGTGACTCTG AGCAGCCCAG CACAGGGTGT CTCCTGCGTG TCCCAGATGA 
     4021 AACCTCCTCA GAATCCCGAC TTTCTGACCC AGATTCAGAG ACGTTCTGTG CTGGACTGCT 
     4081 CCATTGCTGA CTGCCTGCAC TTCCGCTGTG ACATCCCCTC CTTGGACATC CAGGATGAAC 
     4141 TTGACTTCAT TCTGAGGGGC AACCTCAGCT TCGGCTGGGT CAGTCAGACA TTGCAGGAAA 
     4201 AGGTGTTGCT TGTGAGTGAG GCTGAAATCA CTTTCGACAC ATCTGTGTAC TCCCAGCTGC 
     4261 CAGGACAGGA GGCATTTCTG AGAGCCCAGG TGGAGACAAC GTTAGAAGAA TACGTGGTCT 
     4321 ATGAGCCCAT CTTCCTCGTG GCGGGCAGCT CGGTGGGAGG TCTGCTGTTA CTGGCTCTCA 
     4381 TCACAGTGGT ACTGTACAAG CTTGGCTTCT TCAAACGTCA GTACAAAGAA ATGCTGGACG 
     4441 GCAAGGCTGC AGATCCTGTC ACAGCCGGCC AGGCAGATTT CGGCTGTGAG ACTCCTCCAT 
     4501 ATCTCGTGAG CACGCGTACG CGGCCGCTCG AGCAGAAACT CATCTCAGAA GAGGATCTGG 
     4561 CAGCAAATGA TATCCTGGAT TACAAGGATG ACGACGATAA GGTTTAAACG GCCGGCCGCG 
     4621 GTCATAGCTG TTTCCTGAAC AGATCCCGGG TGGCATCCCT GTGACCCCTC CCCAGTGCCT 
     4681 CTCCTGGCCC TGGAAGTTGC CACTCCAGTG CCCACCAGCC TTGTCCTAAT AAAATTAAGT 
     4741 TGCATCATTT TGTCTGACTA GGTGTCCTTC TATAATATTA TGGGGTGGAG GGGGGTGGTA 
     4801 TGGAGCAAGG GGCAAGTTGG GAAGACAACC TGTAGGGCCT GCGGGGTCTA TTGGGAACCA 
     4861 AGCTGGAGTG CAGTGGCACA ATCTTGGCTC ACTGCAATCT CCGCCTCCTG GGTTCAAGCG 
     4921 ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG GGATTCCAGG CATGCATGAC CAGGCTCAGC 
     4981 TAATTTTTGT TTTTTTGGTA GAGACGGGGT TTCACCATAT TGGCCAGGCT GGTCTCCAAC 
     5041 TCCTAATCTC AGGTGATCTA CCCACCTTGG CCTCCCAAAT TGCTGGGATT ACAGGCGTGA 
     5101 ACCACTGCTC CCTTCCCTGT CCTTCTGATT TTAAAATAAC TATACCAGCA GGAGGACGTC 
     5161 CAGACACAGC ATAGGCTACC TGGCCATGCC CAACCGGTGG GACATTTGAG TTGCTTGCTT 
     5221 GGCACTGTCC TCTCATGCGT TGGGTCCACT CAGTAGATGC CTGTTGAATT GGGTACGCGG 
     5281 CCAGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG 
     5341 GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA 
     5401 GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG 
     5461 ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC 
     5521 TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC 
     5581 CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC 
     5641 GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG 
     5701 CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC 
     5761 ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA 
     5821 GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC 
     5881 TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC 
     5941 CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG 
     6001 ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC 
     6061 ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA 
     6121 TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAACCTGAGG CTATGGCAGG 
     6181 GCCTGCCGCC CCGACGTTGG CTGCGAGCCC TGGGCCTTCA CCCGAACTTG GGGGGTGGGG 
     6241 TGGGGAAAAG GAAGAAACGC GGGCGTATTG GCCCCAATGG GGTCTCGGTG GGGTATCGAC 
     6301 AGAGTGCCAG CCCTGGGACC GAACCCCGCG TTTATGAACA AACGACCCAA CACCGTGCGT 
     6361 TTTATTCTGT CTTTTTATTG CCGTCATAGC GCGGGTTCCT TCCGGTATTG TCTCCTTCCG 
     6421 TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC GAAGAACTCC AGCATGAGAT CCCCGCGCTG 
     6481 GAGGATCATC CAGCCGGCGT CCCGGAAAAC GATTCCGAAG CCCAACCTTT CATAGAAGGC 
     6541 GGCGGTGGAA TCGAAATCTC GTGATGGCAG GTTGGGCGTC GCTTGGTCGG TCATTTCGAA 
     6601 CCCCAGAGTC CCGCTCAGAA GAACTCGTCA AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA 
     6661 TCGGGAGCGG CGATACCGTA AAGCACGAGG AAGCGGTCAG CCCATTCGCC GCCAAGCTCT 
     6721 TCAGCAATAT CACGGGTAGC CAACGCTATG TCCTGATAGC GATCCGCCAC ACCCAGCCGG 
     6781 CCACAGTCGA TGAATCCAGA AAAGCGGCCA TTTTCCACCA TGATATTCGG CAAGCAGGCA 
     6841 TCGCCATGGG TCACGACGAG ATCCTCGCCG TCGGGCATGC TCGCCTTGAG CCTGGCGAAC 
     6901 AGTTCGGCTG GCGCGAGCCC CTGATGCTCT TCGTCCAGAT CATCCTGATC GACAAGACCG 
     6961 GCTTCCATCC GAGTACGTGC TCGCTCGATG CGATGTTTCG CTTGGTGGTC GAATGGGCAG 
     7021 GTAGCCGGAT CAAGCGTATG CAGCCGCCGC ATTGCATCAG CCATGATGGA TACTTTCTCG 
     7081 GCAGGAGCAA GGTGAGATGA CAGGAGATCC TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG 
     7141 TCCCTTCCCG CTTCAGTGAC AACGTCGAGC ACAGCTGCGC AAGGAACGCC CGTCGTGGCC 
     7201 AGCCACGATA GCCGCGCTGC CTCGTCTTGC AGTTCATTCA GGGCACCGGA CAGGTCGGTC 
     7261 TTGACAAAAA GAACCGGGCG CCCCTGCGCT GACAGCCGGA ACACGGCGGC ATCAGAGCAG 
     7321 CCGATTGTCT GTTGTGCCCA GTCATAGCCG AATAGCCTCT CCACCCAAGC GGCCGGAGAA 
     7381 CCTGCGTGCA ATCCATCTTG TTCAATCATG CGAAACGATC CTCATCCTGT CTCTTGATCG 
     7441 ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA GCCTCCTCAC TACTTCTGGA ATAGCTCAGA 
     7501 GGCCGAGGCG GCCTCGGCCT CTGCATAAAT AAAAAAAATT AGTCAGCCAT GGGGCGGAGA 
     7561 ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG GATGGGCGGA GTTAGGGGCG GGACTATGGT 
     7621 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     7681 TCCACACCTG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     7741 GCCTGGGGAC TTTCCACACC CTAACTGACA CACATTCCAC AGCTGGTTCT TTCCGCCTCA 
     7801 GGACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA 
     7861 TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA 
     7921 AAAGTGCCAC CTGACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT GGTGGTTACG 
     7981 CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT 
     8041 TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC TAAATCGGGG GCTCCCTTTA 
     8101 GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA AACTTGATTA GGGTGATGGT 
     8161 TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT GGAGTCCACG 
     8221 TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACCCTAT CTCGGTCTAT 
     8281 TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT GGTTAAAAAA TGAGCTGATT 
     8341 TAACAAAAAT TTAACGCGAA TTTT
//