LOCUS       RR215513                7209 bp    DNA     circular  SYN 
DEFINITION  Cdh5 (Myc-DDK-tagged ORF) - Rat cadherin 5 (Cdh5), (10 ug)
ACCESSION   NM_001107407
VERSION     NM_001107407.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3366..3457 
                     /label="Myc-DDK"  
     misc_feature    3487..4072 
                     /label="PolyA signal"  
     misc_feature    3544..3563 
                     /label="Restriction Enzyme"  
     misc_feature    4348..5020 
                     /label="ColE1"  
     misc_feature    5450..6244 
                     /label="Kan/Neo"  
     misc_feature    6279..6581 
                     /label="SV40 ori"  
     misc_feature    6667..7144 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3348..3354 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3348 
                     /label="Cdh5"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCAGAGGCTC ACAGAGCTGG CCACTGCTTT GGGCACCTTC TTGGGCCTGC 
     1081 TGGCAGTGGC AGCGATGGCA GGCCCCAACA TGCTGCCTGT CCACCAGCGC CAAAAGAGAG 
     1141 ACTGGATTTG GAATCAAATG CACATAGATG AAGAGAAAAA TGAGTCGCTG CCCCACTATG 
     1201 TGGGAAAGAT CAAGTCCAAT GTGAACCGTC AGAATGCTAA GTATGTGCTC CAAGGAGAAC 
     1261 AAGCTGGCAA GATCTTCAGG GTCGATGCTG ACACAGGGGA TGTGCTTGCC TATGAGAGGT 
     1321 TAGACCGGGA AAAAGTCTCT GAGTACTTCC TTACTGCCCT CATTGTGGAC AAGAAGACCA 
     1381 ACAAAAACCT GGAACAACCT TCCAGCTTCA CTGTCAAGGT GCATGATGTA AATGACAACT 
     1441 GGCCTGTGTT TACGCACCAG GTATTCAACG CATCTGTGCC CGAGATGTCA GCTATAGGGA 
     1501 CCTCTGTCAT CCGTGTGACA GCAACGGATG CAGATGACCC TACGGTGGCT GGCCACGCCA 
     1561 CTGTCTTGTA CCAAATGGTG AAAGGAAATG AGTACTTCGC CATCGACAAT TCTGGACTCA 
     1621 TTTTCACAAA GGACAAAAAC TTGGACAGAG AGACACGGGC TGAATACAAG ATCGTGGTGG 
     1681 AAGCACAAGA TGCCCAGGGT CTGCGGGGAG AGTCAGGCAC AGCCACTGTG CTGATCAGAT 
     1741 TGGAAGACAT CAATGACAAC TTCCCCATCT TTACTCAGTC CACATACACA TTTTCGGTGC 
     1801 CTGAAGATAT CCGTGTGGGC AAGCCCTTGG GCTCTCTCTC TGTTGAGGAC CCAGATGAGC 
     1861 CTCAGAACCG GATGACCAAA TATAGCATCA TGCAGGGCGA GTACAGGGAC ACCTTCACCA 
     1921 TTGAGACAGA CCCCGACCGT AATGAGGGTA TCATCAAACC CACAAAGCCC CTGGACTATG 
     1981 AACTCATCCA GCAGTACACG TTCCACATCG AGGCCACAGA CCCCACTATC AGACTCGGAT 
     2041 ACCTGAGCAG CACTGCGGGC AAAAACAAAG CCAAGATCAT CATCAATGTC CTAGATGTGG 
     2101 ATGAGCCCCC TGTCTTCCAG CGACGCTTCT ACCACTTCCA CCTGCCCGAA AACAAGAAGA 
     2161 AACCACTGAT TGGCACTGTG GTGGCCAAAG ACCCCGACAA GGCTCAACGC AGCATAGGAT 
     2221 ACTCCATCCG CAAGACCAGT GACAGAGGCC AATACTTCCG AATAACCAAA CAAGGGAACA 
     2281 TCTATAATGA GAAAGAACTG GACAGAGAAA CCCACGCCTG GTATAACCTG ACTGTGGAGG 
     2341 CCAACGAATT GGATTCTAGG GGTAACCCTG TAGGTAAAGA ATCCATTGTA CAGATTTACA 
     2401 TTGAAGTTTT GGATGAGAAT GACAACGCCC CAGAATTTGC CCAGCCCTAC GAACCTAAAG 
     2461 TATGTGAGAA TGCTGCCCAG GGCAAGCTGG TAGTACAGAT CTCTGCAACA GACAAGGACG 
     2521 TGGTGCCAGT AAACACAAAG TTCAAGTTTG CCCTGAAGAA CGAGGACAGC AACTTCACCC 
     2581 TCATCAACAA CCACGACAAT ACCGCCAACA TCACAGTCAA GTATGGACAG TTTAATCGGG 
     2641 AGCATACCAA GTTCCACTAC CTGCCTGTGC TCATCTCCGA CAATGGGATG CCCAGCCTGA 
     2701 CTGGAACCGG CACGCTAACA GTGGCTGTGT GCAAATGCAA CGAACAGGGC GAGTTCACCT 
     2761 TCTGTGAGGA GATGGCCGCA CAGGCGGGTG TCAGCATCCA GGCACTGGTA GCCATCTTTC 
     2821 TGTGCATCCT CACCATCACA GTGATCACCT TGCTGATCTT CCTGCGGAGG CGGCTCCGGA 
     2881 AGCAGGCGCA TGCTCATAGC AAGAGTGCGC TTGAGATTCA TGAGCAGTTG GTCACCTACG 
     2941 ACGAGGAGGG CGGTGGCGAG ATGGACACCA CAAGCTACGA CGTGTCTGTG CTTAATTCTG 
     3001 TGCGCACCGG CTCCAACAAG CCTCTGCGGT CCACGATGGA TGCCCGGCCA GCAGTATACA 
     3061 CACAGGTGCA GAAGCCTCCA CGACTGGCGC CTAGCCTGCA CGGAGGGCCC AGAGAGATGG 
     3121 CCACCATGAT CGAGGTGAAG AAAGAAGAGG CAGACAATGA TGGCGGTGGT CCCCCCTACG 
     3181 ATACACTGCA CATCTACGGC TACGAGGGCA CAGAGTCCAT CGCAGAGTCC CTCAGCTCCC 
     3241 TGAGCACCAA TTCCTCCGAC TCTGACATCG ACTATGACTT CCTCAATGAC TGGGGACCCA 
     3301 GGTTCAAGAT GCTGGCAGAG CTGTATGGCT CAGATCCTCA GGAAGAGCTC ATCATCACGC 
     3361 GTACGCGGCC GCTCGAGCAG AAACTCATCT CAGAAGAGGA TCTGGCAGCA AATGATATCC 
     3421 TGGATTACAA GGATGACGAC GATAAGGTTT AAACGGCCGG CCGCGGTCAT AGCTGTTTCC 
     3481 TGAACAGATC CCGGGTGGCA TCCCTGTGAC CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA 
     3541 GTTGCCACTC CAGTGCCCAC CAGCCTTGTC CTAATAAAAT TAAGTTGCAT CATTTTGTCT 
     3601 GACTAGGTGT CCTTCTATAA TATTATGGGG TGGAGGGGGG TGGTATGGAG CAAGGGGCAA 
     3661 GTTGGGAAGA CAACCTGTAG GGCCTGCGGG GTCTATTGGG AACCAAGCTG GAGTGCAGTG 
     3721 GCACAATCTT GGCTCACTGC AATCTCCGCC TCCTGGGTTC AAGCGATTCT CCTGCCTCAG 
     3781 CCTCCCGAGT TGTTGGGATT CCAGGCATGC ATGACCAGGC TCAGCTAATT TTTGTTTTTT 
     3841 TGGTAGAGAC GGGGTTTCAC CATATTGGCC AGGCTGGTCT CCAACTCCTA ATCTCAGGTG 
     3901 ATCTACCCAC CTTGGCCTCC CAAATTGCTG GGATTACAGG CGTGAACCAC TGCTCCCTTC 
     3961 CCTGTCCTTC TGATTTTAAA ATAACTATAC CAGCAGGAGG ACGTCCAGAC ACAGCATAGG 
     4021 CTACCTGGCC ATGCCCAACC GGTGGGACAT TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA 
     4081 TGCGTTGGGT CCACTCAGTA GATGCCTGTT GAATTGGGTA CGCGGCCAGC GGCGAGCGGT 
     4141 ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA 
     4201 GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC 
     4261 GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG 
     4321 GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT 
     4381 GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG 
     4441 AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG 
     4501 CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG 
     4561 TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC 
     4621 TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG 
     4681 GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT 
     4741 TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG 
     4801 TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC 
     4861 TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT 
     4921 GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT 
     4981 TAAATCAATC TAAAGTATAT ATGAGTAACC TGAGGCTATG GCAGGGCCTG CCGCCCCGAC 
     5041 GTTGGCTGCG AGCCCTGGGC CTTCACCCGA ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA 
     5101 AACGCGGGCG TATTGGCCCC AATGGGGTCT CGGTGGGGTA TCGACAGAGT GCCAGCCCTG 
     5161 GGACCGAACC CCGCGTTTAT GAACAAACGA CCCAACACCG TGCGTTTTAT TCTGTCTTTT 
     5221 TATTGCCGTC ATAGCGCGGG TTCCTTCCGG TATTGTCTCC TTCCGTGTTT CAGTTAGCCT 
     5281 CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT GAGATCCCCG CGCTGGAGGA TCATCCAGCC 
     5341 GGCGTCCCGG AAAACGATTC CGAAGCCCAA CCTTTCATAG AAGGCGGCGG TGGAATCGAA 
     5401 ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG GTCGGTCATT TCGAACCCCA GAGTCCCGCT 
     5461 CAGAAGAACT CGTCAAGAAG GCGATAGAAG GCGATGCGCT GCGAATCGGG AGCGGCGATA 
     5521 CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT TCGCCGCCAA GCTCTTCAGC AATATCACGG 
     5581 GTAGCCAACG CTATGTCCTG ATAGCGATCC GCCACACCCA GCCGGCCACA GTCGATGAAT 
     5641 CCAGAAAAGC GGCCATTTTC CACCATGATA TTCGGCAAGC AGGCATCGCC ATGGGTCACG 
     5701 ACGAGATCCT CGCCGTCGGG CATGCTCGCC TTGAGCCTGG CGAACAGTTC GGCTGGCGCG 
     5761 AGCCCCTGAT GCTCTTCGTC CAGATCATCC TGATCGACAA GACCGGCTTC CATCCGAGTA 
     5821 CGTGCTCGCT CGATGCGATG TTTCGCTTGG TGGTCGAATG GGCAGGTAGC CGGATCAAGC 
     5881 GTATGCAGCC GCCGCATTGC ATCAGCCATG ATGGATACTT TCTCGGCAGG AGCAAGGTGA 
     5941 GATGACAGGA GATCCTGCCC CGGCACTTCG CCCAATAGCA GCCAGTCCCT TCCCGCTTCA 
     6001 GTGACAACGT CGAGCACAGC TGCGCAAGGA ACGCCCGTCG TGGCCAGCCA CGATAGCCGC 
     6061 GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA CCGGACAGGT CGGTCTTGAC AAAAAGAACC 
     6121 GGGCGCCCCT GCGCTGACAG CCGGAACACG GCGGCATCAG AGCAGCCGAT TGTCTGTTGT 
     6181 GCCCAGTCAT AGCCGAATAG CCTCTCCACC CAAGCGGCCG GAGAACCTGC GTGCAATCCA 
     6241 TCTTGTTCAA TCATGCGAAA CGATCCTCAT CCTGTCTCTT GATCGATCTT TGCAAAAGCC 
     6301 TAGGCCTCCA AAAAAGCCTC CTCACTACTT CTGGAATAGC TCAGAGGCCG AGGCGGCCTC 
     6361 GGCCTCTGCA TAAATAAAAA AAATTAGTCA GCCATGGGGC GGAGAATGGG CGGAACTGGG 
     6421 CGGAGTTAGG GGCGGGATGG GCGGAGTTAG GGGCGGGACT ATGGTTGCTG ACTAATTGAG 
     6481 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCTGGTTGC 
     6541 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     6601 ACACCCTAAC TGACACACAT TCCACAGCTG GTTCTTTCCG CCTCAGGACT CTTCCTTTTT 
     6661 CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT 
     6721 ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC 
     6781 GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG TTACGCGCAG CGTGACCGCT 
     6841 ACACTTGCCA GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT TCTCGCCACG 
     6901 TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC CTTTAGGGTT CCGATTTAGT 
     6961 GCTTTACGGC ACCTCGACCC CAAAAAACTT GATTAGGGTG ATGGTTCACG TAGTGGGCCA 
     7021 TCGCCCTGAT AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA 
     7081 CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG TCTATTCTTT TGATTTATAA 
     7141 GGGATTTTGC CGATTTCGGC CTATTGGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC 
     7201 GCGAATTTT
//