LOCUS       RR215421                6672 bp    DNA     circular  SYN 
DEFINITION  Bche (Myc-DDK-tagged ORF) - Rat butyrylcholinesterase (Bche), (10 ug)
ACCESSION   NM_022942
VERSION     NM_022942.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    2829..2920 
                     /label="Myc-DDK"  
     misc_feature    2950..3535 
                     /label="PolyA signal"  
     misc_feature    3007..3026 
                     /label="Restriction Enzyme"  
     misc_feature    3811..4483 
                     /label="ColE1"  
     misc_feature    4913..5707 
                     /label="Kan/Neo"  
     misc_feature    5742..6044 
                     /label="SV40 ori"  
     misc_feature    6130..6607 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    2811..2817 
                     /label="Restriction Enzyme"  
     misc_feature    1020..2811 
                     /label="Bche"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGTCACAGAA ATTCACTTCC TCCTATGGAT TCTTCTGCTC TGCATGCTTT 
     1081 TTGGGAAGTC ACACACTGAA GAAGACGTCA TAATTACAAC CAAGACTGGA AGAGTCCGAG 
     1141 GGCTGAGCAT GCCCATTCTA GGTGGCACAG TGACTGCCTT TCTCGGTATC CCCTATGCAC 
     1201 AGCCTCCTCT GGGTAGCCTA AGATTCAAAA AGCCACAACC TTTAAACAAA TGGCCTGATG 
     1261 TCTATAATGC CACTAAATAT GCAAATTCTT GTTATCAGAA TATAGATCAA GCCTTCCCAG 
     1321 GCTTCCAGGG GTCAGAAATG TGGAATCCAA ACACGAACCT CAGTGAAGAC TGCTTGTATC 
     1381 TGAATGTTTG GATTCCAGTA CCTAAGCCCA AGAATGCGAC TGTCATGGTG TGGGTCTATG 
     1441 GTGGTGGCTT TCAAACTGGG ACCTCCTCTC TACCCGTTTA TGATGGGAAA TTTCTCACTC 
     1501 GTGTTGAAAG AGTTATTGTA GTTTCAATGA ACTATAGGGT AGGTGCCCTA GGATTCCTAG 
     1561 CTTTTCCAGG AAATTCTGAG GCTCCAGGAA ACATGGGTTT ATTTGATCAA CAGTTGGCAC 
     1621 TTCAGTGGAT CCAAAGAAAT ATAGCTGCTT TCGGAGGGAA TCCTAAAAGT GTAACACTTT 
     1681 TTGGAGAAAG TGCAGGAGCT GCTTCAGTTA GCTTACATTT GCTATGTCCC CAAAGTTACC 
     1741 CTTTGTTTAC TAGAGCCATT CTAGAAAGTG GCTCCTCTAA TGCCCCCTGG GCAGTAAAGC 
     1801 ATCCAGAGGA AGCCAGAAAC AGAACCTTAA CCTTAGCTAA ATTTATTGGT TGCTCAAAGG 
     1861 AGAATGAGAA GGAGATAATT ACATGCCTTC GAAGTAAAGA TCCTCAGGAA ATTCTTCTGA 
     1921 ATGAAAAGTT AGTTCTCCCC TCTGATTCCA TCAGATCGAT TAATTTTGGT CCAACAGTGG 
     1981 ATGGCGATTT TCTCACTGAT ATGCCCCACA CACTACTCCA ACTTGGAAAA GTGAAAACAG 
     2041 CTCAGATCTT AGTGGGCGTT AACAAGGATG AAGGGACAGC TTTCCTAGTG TACGGTGCTC 
     2101 CTGGTTTCAG CAAAGACAAT GATAGTCTTA TCACACGGAG GGAATTTCAA GAAGGTTTAA 
     2161 ATATGTATTT CCCTGGAGTG AGCAGCTTGG GAAAGGAAGC AATCCTTTTC TACTATGTGG 
     2221 ACTGGTTAGG TGACCAGACA CCGGAAGTCT ACCGTGAAGC TTTCGATGAT ATTATTGGAG 
     2281 ATTACAACAT CATATGCCCT GCACTGGAGT TTACCAAGAA ATTTGCAGAA CTAGAAATTA 
     2341 ATGCCTTTTT CTACTATTTT GAACACCGAT CTTCCAAACT ACCTTGGCCA GAATGGATGG 
     2401 GAGTAATGCA TGGCTATGAA ATTGAATTTG TATTTGGCTT ACCCCTGGAA AGAAGAGTTA 
     2461 ATTACACAAG GGCTGAGGAA ATCTTTAGTC GATCCATAAT GAAAACTTGG GCAAATTTTG 
     2521 CAAAATATGG ACATCCCAAT GGGACTCAGG GCAATAGCAC AGTGTGGCCT GTCTTCACAA 
     2581 GTACTGAACA AAAATACCTA ACCTTGAACA CAGAGAAGTC AAAAATAAAC TCTAAACTCC 
     2641 GGGCTCCCCA GTGCCAATTC TGGAGACTAT TTTTTCCAAA AGTCTTGGAA ATAACAGGAG 
     2701 ATATTGATGA AAGGGAGCAA GAGTGGAAGG CAGGATTCCA TCGCTGGAGC AATTACATGA 
     2761 TGGACTGGAA AAATCAATTT AACGATTACA CTAGCAAGAA AGAGACTTGT ACAGATCTCA 
     2821 CGCGTACGCG GCCGCTCGAG CAGAAACTCA TCTCAGAAGA GGATCTGGCA GCAAATGATA 
     2881 TCCTGGATTA CAAGGATGAC GACGATAAGG TTTAAACGGC CGGCCGCGGT CATAGCTGTT 
     2941 TCCTGAACAG ATCCCGGGTG GCATCCCTGT GACCCCTCCC CAGTGCCTCT CCTGGCCCTG 
     3001 GAAGTTGCCA CTCCAGTGCC CACCAGCCTT GTCCTAATAA AATTAAGTTG CATCATTTTG 
     3061 TCTGACTAGG TGTCCTTCTA TAATATTATG GGGTGGAGGG GGGTGGTATG GAGCAAGGGG 
     3121 CAAGTTGGGA AGACAACCTG TAGGGCCTGC GGGGTCTATT GGGAACCAAG CTGGAGTGCA 
     3181 GTGGCACAAT CTTGGCTCAC TGCAATCTCC GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT 
     3241 CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA TGCATGACCA GGCTCAGCTA ATTTTTGTTT 
     3301 TTTTGGTAGA GACGGGGTTT CACCATATTG GCCAGGCTGG TCTCCAACTC CTAATCTCAG 
     3361 GTGATCTACC CACCTTGGCC TCCCAAATTG CTGGGATTAC AGGCGTGAAC CACTGCTCCC 
     3421 TTCCCTGTCC TTCTGATTTT AAAATAACTA TACCAGCAGG AGGACGTCCA GACACAGCAT 
     3481 AGGCTACCTG GCCATGCCCA ACCGGTGGGA CATTTGAGTT GCTTGCTTGG CACTGTCCTC 
     3541 TCATGCGTTG GGTCCACTCA GTAGATGCCT GTTGAATTGG GTACGCGGCC AGCGGCGAGC 
     3601 GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA GAATCAGGGG ATAACGCAGG 
     3661 AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT 
     3721 GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA 
     3781 GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT 
     3841 CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC 
     3901 GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT 
     3961 TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC 
     4021 CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC 
     4081 CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG 
     4141 GTGGCCTAAC TACGGCTACA CTAGAAGAAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC 
     4201 AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG 
     4261 CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA 
     4321 TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT 
     4381 TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG 
     4441 TTTTAAATCA ATCTAAAGTA TATATGAGTA ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC 
     4501 GACGTTGGCT GCGAGCCCTG GGCCTTCACC CGAACTTGGG GGGTGGGGTG GGGAAAAGGA 
     4561 AGAAACGCGG GCGTATTGGC CCCAATGGGG TCTCGGTGGG GTATCGACAG AGTGCCAGCC 
     4621 CTGGGACCGA ACCCCGCGTT TATGAACAAA CGACCCAACA CCGTGCGTTT TATTCTGTCT 
     4681 TTTTATTGCC GTCATAGCGC GGGTTCCTTC CGGTATTGTC TCCTTCCGTG TTTCAGTTAG 
     4741 CCTCCCCCTA GGGTGGGCGA AGAACTCCAG CATGAGATCC CCGCGCTGGA GGATCATCCA 
     4801 GCCGGCGTCC CGGAAAACGA TTCCGAAGCC CAACCTTTCA TAGAAGGCGG CGGTGGAATC 
     4861 GAAATCTCGT GATGGCAGGT TGGGCGTCGC TTGGTCGGTC ATTTCGAACC CCAGAGTCCC 
     4921 GCTCAGAAGA ACTCGTCAAG AAGGCGATAG AAGGCGATGC GCTGCGAATC GGGAGCGGCG 
     4981 ATACCGTAAA GCACGAGGAA GCGGTCAGCC CATTCGCCGC CAAGCTCTTC AGCAATATCA 
     5041 CGGGTAGCCA ACGCTATGTC CTGATAGCGA TCCGCCACAC CCAGCCGGCC ACAGTCGATG 
     5101 AATCCAGAAA AGCGGCCATT TTCCACCATG ATATTCGGCA AGCAGGCATC GCCATGGGTC 
     5161 ACGACGAGAT CCTCGCCGTC GGGCATGCTC GCCTTGAGCC TGGCGAACAG TTCGGCTGGC 
     5221 GCGAGCCCCT GATGCTCTTC GTCCAGATCA TCCTGATCGA CAAGACCGGC TTCCATCCGA 
     5281 GTACGTGCTC GCTCGATGCG ATGTTTCGCT TGGTGGTCGA ATGGGCAGGT AGCCGGATCA 
     5341 AGCGTATGCA GCCGCCGCAT TGCATCAGCC ATGATGGATA CTTTCTCGGC AGGAGCAAGG 
     5401 TGAGATGACA GGAGATCCTG CCCCGGCACT TCGCCCAATA GCAGCCAGTC CCTTCCCGCT 
     5461 TCAGTGACAA CGTCGAGCAC AGCTGCGCAA GGAACGCCCG TCGTGGCCAG CCACGATAGC 
     5521 CGCGCTGCCT CGTCTTGCAG TTCATTCAGG GCACCGGACA GGTCGGTCTT GACAAAAAGA 
     5581 ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC ACGGCGGCAT CAGAGCAGCC GATTGTCTGT 
     5641 TGTGCCCAGT CATAGCCGAA TAGCCTCTCC ACCCAAGCGG CCGGAGAACC TGCGTGCAAT 
     5701 CCATCTTGTT CAATCATGCG AAACGATCCT CATCCTGTCT CTTGATCGAT CTTTGCAAAA 
     5761 GCCTAGGCCT CCAAAAAAGC CTCCTCACTA CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC 
     5821 CTCGGCCTCT GCATAAATAA AAAAAATTAG TCAGCCATGG GGCGGAGAAT GGGCGGAACT 
     5881 GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT TAGGGGCGGG ACTATGGTTG CTGACTAATT 
     5941 GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCTGGT 
     6001 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     6061 TCCACACCCT AACTGACACA CATTCCACAG CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT 
     6121 TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA 
     6181 TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT 
     6241 GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC 
     6301 GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC 
     6361 ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT 
     6421 AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG 
     6481 CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT 
     6541 GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA 
     6601 TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT 
     6661 AACGCGAATT TT
//