LOCUS       RR215355                7785 bp    DNA     circular  SYN 
DEFINITION  Daam1 (Myc-DDK-tagged ORF) - Rat dishevelled associated activator of morphogenesis 1 (Daam1), (10 ug)
ACCESSION   NM_001108030
VERSION     NM_001108030.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3942..4033 
                     /label="Myc-DDK"  
     misc_feature    4063..4648 
                     /label="PolyA signal"  
     misc_feature    4120..4139 
                     /label="Restriction Enzyme"  
     misc_feature    4924..5596 
                     /label="ColE1"  
     misc_feature    6026..6820 
                     /label="Kan/Neo"  
     misc_feature    6855..7157 
                     /label="SV40 ori"  
     misc_feature    7243..7720 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3924..3930 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3924 
                     /label="Daam1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCCCCAAGG AAGAGAGGCG GAAGGGGGAT TTCATTTATC TTTTGCTGTT 
     1081 TCCGAAACAA TGACCACCCA GAAATCACAT ATCGGCTTAG GAATGATAGC AACTTTGCAC 
     1141 TTCAGACAAT GGAGCCAGCA CTGCCCATGC CCCCTGTGGA GGAGCTGGAT GTCATGTTCA 
     1201 GTGAACTTGT GGATGAACTC GACCTCACAG ACAAACATAG GGAGGCCATG TTTGCACTTC 
     1261 CAGCAGAGAA AAAGTGGCAG ATATACTGTA GCAAGAAAAA GGACCAGGAA GAAAACAAGG 
     1321 GAGCAACAAG TTGGCCCGAG TTCTACATTG ATCAGCTCAA TTCCATGGCT GCTAGAAAAT 
     1381 CTCTGCTGGC TTTAGAGAAG GAAGAAGAAG AAGAGAGGAG TAAAACCATC GAGAGTCTAA 
     1441 AGACAGCACT GAGGACAAAG CCCATGAGGT TTGTAACCAG ATTCATCGAC TTGGATGGCC 
     1501 TGTCATGTAT TCTCAACTTT CTGAAGACCA TGGACTATGA GACCTCCGAG TCTCGGATAC 
     1561 ATACCTCCCT CATTGGCTGT ATAAAGGCAC TAATGAACAA CTCTCAAGGC CGGGCCCACG 
     1621 TCCTGGCTCA TTCTGAGAGT ATTAATGTAA TTGCTCAGAG TCTGAGCACA GAGAACATTA 
     1681 AAACAAAGGT GGCCGTGCTG GAAATCCTGG GCGCTGTGTG CCTGGTTCCT GGGGGCCACA 
     1741 AGAAAGTTCT GCAGGCTATG CTGCACTATC AGAAGTATGC CAGCGAGAGA ACCCGCTTCC 
     1801 AGACGTTAAT CAACGACTTG GATAAAAGCA CTGGGCGCTA TCGGGATGAA GTGAGTCTCA 
     1861 AGACTGCCAT CATGTCGTTC ATCAATGCAG TGCTGAGCCA GGGAGCCGGA GTGGAGAGTT 
     1921 TGGACTTCAG ACTCCATCTT CGCTACGAAT TTCTGATGCT AGGAATTCAG CCTGTTATAG 
     1981 ATAAATTAAG GGAACATGAG AATTCAACAC TAGACAGGCA CTTAGACTTT TTTGAAATGC 
     2041 TCCGAAATGA AGATGAACTA GAATTTGCCA AAAGATTTGA ACTGGTTCAC ATAGACACAA 
     2101 AAAGCGCCAC TCAGATGTTT GAGCTGACCA GGAGGAGGCT CACCCACAGC GAAGCCTACC 
     2161 CCCACTTCAT GTCCATTCTG CACCATTGCC TGCAGATGCC TTACAAGAGG AGTGGTAACA 
     2221 CAGTCCAGTA CTGGCTGCTG CTAGACAGAA TCATACAGCA GATAGTTATC CAGAATGACA 
     2281 AAGGACAAGA CCCCGACTCC ACACCTCTGG AAAACTTTAA TATTAAGAAC GTCGTAAGAA 
     2341 TGCTGGTTAA TGAAAACGAA GTTAAGCAAT GGAAAGAGCA AGCAGAGAAG ATGAGGAAAG 
     2401 AGCACAATGA GCTACAACAG AAGCTGGAGA AGAAAGAGAG AGAATGTGAT GCCAAGACCC 
     2461 AAGAGAAGGA AGAAATGATG CAGACCTTAA ACAAGATGAA GGAGAAGCTG GAGAAGGAGA 
     2521 CCACTGAGCA CAAGCAGGTT AAGCAGCAGG TGGCAGACCT CACGGCGCAG CTCCACGAGC 
     2581 TCAACAGGAA CAAATTGGAA GGAACAGTAT GGACTGAAAT CGATGATACA AAAGTCTTCA 
     2641 AAATTCTAGA CCTTGAAGAC CTAGAAAGAA CATTCTCTGC CTACCAAAGA CAGCAGAAAG 
     2701 AAGCAGATGC CATTGATGAC GCACTGAGTT CCAAACTTAA AGTCAAAGAG CTGTCAGTGA 
     2761 TTGATGGTCG GAGAGCTCAG AATTGCAACA TCCTTCTGTC GAGGTTGAAG TTATCCAATG 
     2821 ACGAAATCAA GCGAGCAATT CTAACAATGG ATGAACAGGA GGACCTGCCC AAGGACATGT 
     2881 TAGAACAGCT TTTGAAATTC GTGCCTGAGA AAAGCGACAT TGACCTGCTA GAAGAACATA 
     2941 AACATGAGTT GGACCGGATG GCCAAGGCTG ATCGCTTCCT GTTTGAGATG AGCAGAATTA 
     3001 ATCATTACCA GCAACGACTG CAATCACTGT ACTTCAAAAA GAAGTTTGCA GAGCGTGTGG 
     3061 CCGAAGTGAA GCCCAAAGTA GAAGCGATCC GCTCCAGCTC AGAGGAGGTA CTCAGGAGCA 
     3121 GTGCCCTCAA GCAGCTCCTG GAGGTGGTTC TGGCTTTTGG AAATTATATG AATAAAGGAC 
     3181 AAAGAGGCAA TGCATATGGA TTCAAGATCT CCAGCCTCAA CAAGATTGCA GATACAAAAT 
     3241 CCAGTATTGA CAAGAACATT ACCCTCTTGC ACTATCTAAT AACCATTGTG GAGAATAAGT 
     3301 ACCCCAAGGT CCTGAATCTC AGCGAAGAGT TGCGGGATAT TCCTCAAGCA GCGAAAGTGA 
     3361 ACATGACTGA GCTGGACAAA GAGATCAGCA CCTTGAGGAG TGGCCTGAAA GCTGTGGAGA 
     3421 CGGAACTTGA GTATCAAAAG TCTCAGCCCC CGCAGCCTGG AGACAAGTTT GTGTCTGTTG 
     3481 TCAGCCAGTT TATCACCCTG GCGAGCTTCA GCTTCTCAGA CGTAGAAGAT CTTCTAGCAG 
     3541 AAGCTAAAGA GCTGTTCACT AAAGCAGTGA AGCACTTTGG GGAAGAGGCT GGCAAAATCC 
     3601 AGCCAGATGA GTTCTTTGGG ATCTTTGATC AGTTTCTTCA GGCTGTGACA GAAGCGAAAC 
     3661 AGGAAAATGA GAACATGAGG AAAAGGAAGG AGGAGGAGGA GCGGAGGGCT CGCCTGGAAG 
     3721 CTCAGCTCAA AGAGCAGCGG GAGAGGGAGC GGAAGATGAG GAAGGCCAAG GAGAGCAGTG 
     3781 AAGAGAGTGG CGAGTTTGAC GACCTGGTCT CGGCTCTGCG CTCAGGAGAG GTGTTCGACA 
     3841 AAGACCTTTC AAAACTCAAA CGGAACCGGA AACGCATTTC CAACCAGGTG ACGGACAGCA 
     3901 GCCGCGAGCG ACCAATCACG AAACTTAATT TTACGCGTAC GCGGCCGCTC GAGCAGAAAC 
     3961 TCATCTCAGA AGAGGATCTG GCAGCAAATG ATATCCTGGA TTACAAGGAT GACGACGATA 
     4021 AGGTTTAAAC GGCCGGCCGC GGTCATAGCT GTTTCCTGAA CAGATCCCGG GTGGCATCCC 
     4081 TGTGACCCCT CCCCAGTGCC TCTCCTGGCC CTGGAAGTTG CCACTCCAGT GCCCACCAGC 
     4141 CTTGTCCTAA TAAAATTAAG TTGCATCATT TTGTCTGACT AGGTGTCCTT CTATAATATT 
     4201 ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG GGGCAAGTTG GGAAGACAAC CTGTAGGGCC 
     4261 TGCGGGGTCT ATTGGGAACC AAGCTGGAGT GCAGTGGCAC AATCTTGGCT CACTGCAATC 
     4321 TCCGCCTCCT GGGTTCAAGC GATTCTCCTG CCTCAGCCTC CCGAGTTGTT GGGATTCCAG 
     4381 GCATGCATGA CCAGGCTCAG CTAATTTTTG TTTTTTTGGT AGAGACGGGG TTTCACCATA 
     4441 TTGGCCAGGC TGGTCTCCAA CTCCTAATCT CAGGTGATCT ACCCACCTTG GCCTCCCAAA 
     4501 TTGCTGGGAT TACAGGCGTG AACCACTGCT CCCTTCCCTG TCCTTCTGAT TTTAAAATAA 
     4561 CTATACCAGC AGGAGGACGT CCAGACACAG CATAGGCTAC CTGGCCATGC CCAACCGGTG 
     4621 GGACATTTGA GTTGCTTGCT TGGCACTGTC CTCTCATGCG TTGGGTCCAC TCAGTAGATG 
     4681 CCTGTTGAAT TGGGTACGCG GCCAGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT 
     4741 ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA 
     4801 AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC 
     4861 TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA 
     4921 AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC 
     4981 GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC 
     5041 ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA 
     5101 ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC 
     5161 GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG 
     5221 GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG 
     5281 AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG 
     5341 CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA 
     5401 GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA 
     5461 CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT 
     5521 CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA 
     5581 GTAACCTGAG GCTATGGCAG GGCCTGCCGC CCCGACGTTG GCTGCGAGCC CTGGGCCTTC 
     5641 ACCCGAACTT GGGGGGTGGG GTGGGGAAAA GGAAGAAACG CGGGCGTATT GGCCCCAATG 
     5701 GGGTCTCGGT GGGGTATCGA CAGAGTGCCA GCCCTGGGAC CGAACCCCGC GTTTATGAAC 
     5761 AAACGACCCA ACACCGTGCG TTTTATTCTG TCTTTTTATT GCCGTCATAG CGCGGGTTCC 
     5821 TTCCGGTATT GTCTCCTTCC GTGTTTCAGT TAGCCTCCCC CTAGGGTGGG CGAAGAACTC 
     5881 CAGCATGAGA TCCCCGCGCT GGAGGATCAT CCAGCCGGCG TCCCGGAAAA CGATTCCGAA 
     5941 GCCCAACCTT TCATAGAAGG CGGCGGTGGA ATCGAAATCT CGTGATGGCA GGTTGGGCGT 
     6001 CGCTTGGTCG GTCATTTCGA ACCCCAGAGT CCCGCTCAGA AGAACTCGTC AAGAAGGCGA 
     6061 TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG GCGATACCGT AAAGCACGAG GAAGCGGTCA 
     6121 GCCCATTCGC CGCCAAGCTC TTCAGCAATA TCACGGGTAG CCAACGCTAT GTCCTGATAG 
     6181 CGATCCGCCA CACCCAGCCG GCCACAGTCG ATGAATCCAG AAAAGCGGCC ATTTTCCACC 
     6241 ATGATATTCG GCAAGCAGGC ATCGCCATGG GTCACGACGA GATCCTCGCC GTCGGGCATG 
     6301 CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT GGCGCGAGCC CCTGATGCTC TTCGTCCAGA 
     6361 TCATCCTGAT CGACAAGACC GGCTTCCATC CGAGTACGTG CTCGCTCGAT GCGATGTTTC 
     6421 GCTTGGTGGT CGAATGGGCA GGTAGCCGGA TCAAGCGTAT GCAGCCGCCG CATTGCATCA 
     6481 GCCATGATGG ATACTTTCTC GGCAGGAGCA AGGTGAGATG ACAGGAGATC CTGCCCCGGC 
     6541 ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC GCTTCAGTGA CAACGTCGAG CACAGCTGCG 
     6601 CAAGGAACGC CCGTCGTGGC CAGCCACGAT AGCCGCGCTG CCTCGTCTTG CAGTTCATTC 
     6661 AGGGCACCGG ACAGGTCGGT CTTGACAAAA AGAACCGGGC GCCCCTGCGC TGACAGCCGG 
     6721 AACACGGCGG CATCAGAGCA GCCGATTGTC TGTTGTGCCC AGTCATAGCC GAATAGCCTC 
     6781 TCCACCCAAG CGGCCGGAGA ACCTGCGTGC AATCCATCTT GTTCAATCAT GCGAAACGAT 
     6841 CCTCATCCTG TCTCTTGATC GATCTTTGCA AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA 
     6901 CTACTTCTGG AATAGCTCAG AGGCCGAGGC GGCCTCGGCC TCTGCATAAA TAAAAAAAAT 
     6961 TAGTCAGCCA TGGGGCGGAG AATGGGCGGA ACTGGGCGGA GTTAGGGGCG GGATGGGCGG 
     7021 AGTTAGGGGC GGGACTATGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     7081 TGCTGGGGAG CCTGGGGACT TTCCACACCT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     7141 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CCTAACTGAC ACACATTCCA 
     7201 CAGCTGGTTC TTTCCGCCTC AGGACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG 
     7261 GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG 
     7321 GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGACGCGC CCTGTAGCGG CGCATTAAGC 
     7381 GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG ACCGCTACAC TTGCCAGCGC CCTAGCGCCC 
     7441 GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC GCCACGTTCG CCGGCTTTCC CCGTCAAGCT 
     7501 CTAAATCGGG GGCTCCCTTT AGGGTTCCGA TTTAGTGCTT TACGGCACCT CGACCCCAAA 
     7561 AAACTTGATT AGGGTGATGG TTCACGTAGT GGGCCATCGC CCTGATAGAC GGTTTTTCGC 
     7621 CCTTTGACGT TGGAGTCCAC GTTCTTTAAT AGTGGACTCT TGTTCCAAAC TGGAACAACA 
     7681 CTCAACCCTA TCTCGGTCTA TTCTTTTGAT TTATAAGGGA TTTTGCCGAT TTCGGCCTAT 
     7741 TGGTTAAAAA ATGAGCTGAT TTAACAAAAA TTTAACGCGA ATTTT
//