LOCUS       RR215329                7701 bp    DNA     circular  SYN 
DEFINITION  Safb (Myc-DDK-tagged ORF) - Rat scaffold attachment factor B (Safb), (10 ug)
ACCESSION   NM_022394
VERSION     NM_022394.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3858..3949 
                     /label="Myc-DDK"  
     misc_feature    3979..4564 
                     /label="PolyA signal"  
     misc_feature    4036..4055 
                     /label="Restriction Enzyme"  
     misc_feature    4840..5512 
                     /label="ColE1"  
     misc_feature    5942..6736 
                     /label="Kan/Neo"  
     misc_feature    6771..7073 
                     /label="SV40 ori"  
     misc_feature    7159..7636 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3840..3846 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3840 
                     /label="Safb"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGGAGACT CTGTCGGGTT TAGGAGACTC GGGTGCGGCA AGCGCAGCGG 
     1081 CCGTGAGCTC CGCGGCGTCA GAGACCGGGA CGCGGCGGCT CAGCGACCTG CGGGTGATCG 
     1141 ACCTGCGGGC GGAGCTGCGG AAGCGAAACC TCGACTCGAG CGGCAACAAG AGCGTGCTGA 
     1201 TGGAGCGGCT TAAGAAGGCC ATTGAAGAGG AAGGTGGGAA TCCTGATGAA ATTGAAGTCA 
     1261 TCTCAGAAGG CAACAAGAAG ATGCCTAAGA GGCCCAGCAA AGGGAAGAAA CCAGAAGACG 
     1321 AGGGTGTGGA GGACAACGGG CTCGAGGAGA ATTCTGGGGA TGGGCAGGAG GATGTGGAAA 
     1381 CCAGCCTGGA GAACCTGCAA GACATGGATA TGATGGACAT CAGTGTGCTG GATGAGGCTG 
     1441 ACATTGATAA TGGCAGCGTG GCAGACTGTG TGGAGGAAGA AGAGGAGGCC ACACTCCCTG 
     1501 AGGGCCTAGG AGACAGCACT GAACTGGTAG AAGGCGACTC GAAGGGGCTC CCAGAACAGC 
     1561 TGCAGGAGCA TGCTATAGAT GACAAGGAAG CCATTAACAA TGTAGATACA TCATCATCTG 
     1621 ACTTCACCAT CCTGCAGGAG ATGGAAGAGG CATCTTTGGA GCCAGAAAAT GAGAAAATAC 
     1681 TCGACATTTT GGGGGAAACT TGTAAATCTG AGCCAGTAAA AGAAGAAGGT TCGGAGCTGG 
     1741 AGCAGCCCTT TGCCCAGGCC ACGAGTAGCG TGGGGCCAGA CAGGAAGCTG GCGGAGGAAG 
     1801 AGGACCTATT TGAGAGCTGC GGCCACCCGG AAGAGGAAGA AGAAGAGGAG GAAGAAGAGG 
     1861 AGCAGGAAGA GGAGCAGGAG GAGGAGGGAG ATTTAGCTTT GGCCAGCAGC AGCAAGTCTG 
     1921 AGTCTTCAAG CACTCGATGT CAGTGGAGCG AGGCAGACGC CCTGTTAGCA GTAGTGAAAA 
     1981 GGGAGCCGGC AGAGGCGCCT GGCGGAGGCA CTGGGATGGA CCGCGAGCCT GTAGGGCTAG 
     2041 AGGAGCCAGT TGAACAGAGT AGCACGGCTG CCCAGCTCCC GGAGACCACC AGCCAGGAGC 
     2101 TGGTGCGAGC GCCCACGGCA GCCCCGAGCC CTGAGCCCCG AGATAGCAAA GACGACGTGA 
     2161 AGAAGTTTGC TTTTGACGCT TGTAATGACG TCCCTGCGGC TCCTAAAGAG TCCTCAGCCA 
     2221 GTGAGGGCGC TGATCAGAAA ATGAGTTCTG TGGAAGATGA CTCGGATACA AAAAGGCTTT 
     2281 CCAGAGAGGA GAAGGGTCGC AGCAGCTGTG GTAGGAATTT CTGGGTTAGT GGCCTTTCAT 
     2341 CTACGACCAG AGCCACAGAT CTGAAGAACC TTTTTAGCAG ATACGGGAAG GTGGTGGGAG 
     2401 CTAAGGTTGT GACGAACGCC CGGAGTCCCG GAGCTCGCTG TTACGGCTTT GTCACTATGT 
     2461 CCACAGCTGA AGAGGCAACA AAGTGCATTA ACCATTTGCA TAAGACTGAG CTGCATGGCA 
     2521 AGATGATCTC GGTGGAAAAG GCCAAAAGTG AGCCCGCTGG CAAAAGAGTA CCTGACAGGC 
     2581 GAGATGGCGA CAGCAAGAAA GAGAAGACCA GCACCAGTGA CAGATCTGCA AACCTTAAGA 
     2641 GGGAGGAGAA AGGTGACAGA AAAGACGATG CCAAGAAGAC GGATGATGGG AGCACAGAGA 
     2701 AGAGCAAGGA TGCAGATGAC CAAAAGCCTG GGCCTTCAGA GCGCTCTCGA ACAACCAAGT 
     2761 CAGGAAGCCG TGGCACTGAG CGTACTGTGG TGATGGACAA GTCCAAAGGT GTGCCTGTCA 
     2821 TCAGTGTGAA GACTTCTGGT TCTAAAGAGA GAGCCTCCAA AAGTCAGGAC CGAAAGTCTG 
     2881 TCAGCCGGGA GAAGCGGTCT GTTGTGTCCT TTGACAAGGT TAAAGAGTCT CGAAAGTCCA 
     2941 GAGACTCGGA GTCACGGAGG GAGCGGGAGC GGGAGCGCAG TGAGCGGGAG CAAAGGCTGC 
     3001 AGGCCCAGTG GGAGCGGGAG GAGCGAGAGC GGCTGGAGAT TGCCCGGGAG CGCCTGGCTT 
     3061 TCCACCGACA TAGGCTGGAG CGTGAACGCA TGGAGAGGGA ACGGTTGGAG CGGGAGCGCA 
     3121 TGCATGTGGA ACAGGAGCGG AGGCGTGAGC AAGAGCGCAT TCACCGAGAG CGAGAGGAGC 
     3181 TTCGGCGTCA GCAGGAACTG CGCTATGAAC AGGAGCGGAG GCCTGCTGTG CGCAGGCCCT 
     3241 ATGAAGTAGA TGGCCGGCGA GATGATGCCT ATTGGCCAGA AGCTAAACGG GCTGCGCTGG 
     3301 ATGACCGTTA CCACTCAGAC TTCAGTCGCC AGGACCGCTT TCATGACTTC GACCACAGAG 
     3361 ACAGAGGCCG CTATCCCAAC CACTCTGTGG ATAGGAGAGA AGGTTCACGG TCGATGATGG 
     3421 GAGACAGAGA AGGACAGCAT TACCCTGAGC GCCATGGAGG ACCTGAACGC CATGGCCGGG 
     3481 ACTCTCGAGA TGGCTGGGGC TATGGCTCAA ACAAGAGACT GAGTGAAGGC CGGGGGCTGC 
     3541 CCCCTCCTCC CAGGCGTGAC TGGGGAGAGC ATGGCCGGAG ACTTGAAGAT GACCGGGCAT 
     3601 GGCAGGGCAC AGCTGATGGA GGCATGATGG AGCGTGATCA CAAGAGATGG CAAGGTGGTG 
     3661 AGAGGAGCAT GTCTGGCCAC TCAGGGCCCG GTCACATGAT GAATCGTGGT GGCATGTCAG 
     3721 GGCGAGGCAG TTTTGCCCCC GGAGGAGCTT CCCGTGGCCA TGTGATCCCA CGTGGAGGGA 
     3781 TGCAGGCAGG CTTCGGCGGA CAGAGCCGGG GCAGCAGACC CAGTGATGCC CGCTTCACCC 
     3841 GCCGCTATAC GCGTACGCGG CCGCTCGAGC AGAAACTCAT CTCAGAAGAG GATCTGGCAG 
     3901 CAAATGATAT CCTGGATTAC AAGGATGACG ACGATAAGGT TTAAACGGCC GGCCGCGGTC 
     3961 ATAGCTGTTT CCTGAACAGA TCCCGGGTGG CATCCCTGTG ACCCCTCCCC AGTGCCTCTC 
     4021 CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC ACCAGCCTTG TCCTAATAAA ATTAAGTTGC 
     4081 ATCATTTTGT CTGACTAGGT GTCCTTCTAT AATATTATGG GGTGGAGGGG GGTGGTATGG 
     4141 AGCAAGGGGC AAGTTGGGAA GACAACCTGT AGGGCCTGCG GGGTCTATTG GGAACCAAGC 
     4201 TGGAGTGCAG TGGCACAATC TTGGCTCACT GCAATCTCCG CCTCCTGGGT TCAAGCGATT 
     4261 CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA TTCCAGGCAT GCATGACCAG GCTCAGCTAA 
     4321 TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC ACCATATTGG CCAGGCTGGT CTCCAACTCC 
     4381 TAATCTCAGG TGATCTACCC ACCTTGGCCT CCCAAATTGC TGGGATTACA GGCGTGAACC 
     4441 ACTGCTCCCT TCCCTGTCCT TCTGATTTTA AAATAACTAT ACCAGCAGGA GGACGTCCAG 
     4501 ACACAGCATA GGCTACCTGG CCATGCCCAA CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC 
     4561 ACTGTCCTCT CATGCGTTGG GTCCACTCAG TAGATGCCTG TTGAATTGGG TACGCGGCCA 
     4621 GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA 
     4681 TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC 
     4741 CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG 
     4801 CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG 
     4861 AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT 
     4921 TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT 
     4981 GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG 
     5041 CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT 
     5101 GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT 
     5161 CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT 
     5221 GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC 
     5281 CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC 
     5341 TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG 
     5401 TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA 
     5461 AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA CCTGAGGCTA TGGCAGGGCC 
     5521 TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG GCCTTCACCC GAACTTGGGG GGTGGGGTGG 
     5581 GGAAAAGGAA GAAACGCGGG CGTATTGGCC CCAATGGGGT CTCGGTGGGG TATCGACAGA 
     5641 GTGCCAGCCC TGGGACCGAA CCCCGCGTTT ATGAACAAAC GACCCAACAC CGTGCGTTTT 
     5701 ATTCTGTCTT TTTATTGCCG TCATAGCGCG GGTTCCTTCC GGTATTGTCT CCTTCCGTGT 
     5761 TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA GAACTCCAGC ATGAGATCCC CGCGCTGGAG 
     5821 GATCATCCAG CCGGCGTCCC GGAAAACGAT TCCGAAGCCC AACCTTTCAT AGAAGGCGGC 
     5881 GGTGGAATCG AAATCTCGTG ATGGCAGGTT GGGCGTCGCT TGGTCGGTCA TTTCGAACCC 
     5941 CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA AGGCGATAGA AGGCGATGCG CTGCGAATCG 
     6001 GGAGCGGCGA TACCGTAAAG CACGAGGAAG CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA 
     6061 GCAATATCAC GGGTAGCCAA CGCTATGTCC TGATAGCGAT CCGCCACACC CAGCCGGCCA 
     6121 CAGTCGATGA ATCCAGAAAA GCGGCCATTT TCCACCATGA TATTCGGCAA GCAGGCATCG 
     6181 CCATGGGTCA CGACGAGATC CTCGCCGTCG GGCATGCTCG CCTTGAGCCT GGCGAACAGT 
     6241 TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG TCCAGATCAT CCTGATCGAC AAGACCGGCT 
     6301 TCCATCCGAG TACGTGCTCG CTCGATGCGA TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA 
     6361 GCCGGATCAA GCGTATGCAG CCGCCGCATT GCATCAGCCA TGATGGATAC TTTCTCGGCA 
     6421 GGAGCAAGGT GAGATGACAG GAGATCCTGC CCCGGCACTT CGCCCAATAG CAGCCAGTCC 
     6481 CTTCCCGCTT CAGTGACAAC GTCGAGCACA GCTGCGCAAG GAACGCCCGT CGTGGCCAGC 
     6541 CACGATAGCC GCGCTGCCTC GTCTTGCAGT TCATTCAGGG CACCGGACAG GTCGGTCTTG 
     6601 ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC AGCCGGAACA CGGCGGCATC AGAGCAGCCG 
     6661 ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT AGCCTCTCCA CCCAAGCGGC CGGAGAACCT 
     6721 GCGTGCAATC CATCTTGTTC AATCATGCGA AACGATCCTC ATCCTGTCTC TTGATCGATC 
     6781 TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC TCCTCACTAC TTCTGGAATA GCTCAGAGGC 
     6841 CGAGGCGGCC TCGGCCTCTG CATAAATAAA AAAAATTAGT CAGCCATGGG GCGGAGAATG 
     6901 GGCGGAACTG GGCGGAGTTA GGGGCGGGAT GGGCGGAGTT AGGGGCGGGA CTATGGTTGC 
     6961 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     7021 ACACCTGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     7081 TGGGGACTTT CCACACCCTA ACTGACACAC ATTCCACAGC TGGTTCTTTC CGCCTCAGGA 
     7141 CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC 
     7201 ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA 
     7261 GTGCCACCTG ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG CGGGTGTGGT GGTTACGCGC 
     7321 AGCGTGACCG CTACACTTGC CAGCGCCCTA GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC 
     7381 TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG 
     7441 TTCCGATTTA GTGCTTTACG GCACCTCGAC CCCAAAAAAC TTGATTAGGG TGATGGTTCA 
     7501 CGTAGTGGGC CATCGCCCTG ATAGACGGTT TTTCGCCCTT TGACGTTGGA GTCCACGTTC 
     7561 TTTAATAGTG GACTCTTGTT CCAAACTGGA ACAACACTCA ACCCTATCTC GGTCTATTCT 
     7621 TTTGATTTAT AAGGGATTTT GCCGATTTCG GCCTATTGGT TAAAAAATGA GCTGATTTAA 
     7681 CAAAAATTTA ACGCGAATTT T
//