LOCUS       RR215317                9171 bp    DNA     circular  SYN 
DEFINITION  Dapk1 (Myc-DDK-tagged ORF) - Rat death associated protein kinase 1 (Dapk1), (10 ug)
ACCESSION   NM_001107335
VERSION     NM_001107335.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5328..5419 
                     /label="Myc-DDK"  
     misc_feature    5449..6034 
                     /label="PolyA signal"  
     misc_feature    5506..5525 
                     /label="Restriction Enzyme"  
     misc_feature    6310..6982 
                     /label="ColE1"  
     misc_feature    7412..8206 
                     /label="Kan/Neo"  
     misc_feature    8241..8543 
                     /label="SV40 ori"  
     misc_feature    8629..9106 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5310..5316 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5310 
                     /label="Dapk1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GACTGTGTTC AGGCAGGAAA ACGTGGACGA CTACTACGAC ACCGGCGAGG 
     1081 AACTGGGCAG CGGACAGTTC GCCGTTGTGA AGAAATGTCG TGAGAAAAGT ACCGGTCTTC 
     1141 AGTATGCGGC CAAGTTCATC AAGAAAAGGA GGACCAAGTC CAGCCGGCGG GGCGTGAGCC 
     1201 GAGAGGACAT CGAGCGGGAG GTCAGCATCC TGAAGGAGAT CCGGCACCCA AACGTCATCA 
     1261 CCCTGCATGA GGTCTACGAG AACAAGACAG ACGTCATTCT GATCCTGGAG CTTGTTGCAG 
     1321 GAGGCGAGCT GTTTGACTTC CTGGCTGAGA AGGAATCTCT CACTGAAGAA GAAGCAACCG 
     1381 AGTTCCTCAA GCAGATCCTC AGCGGCGTTT ACTACCTGCA CTCGCTTCAA ATCGCCCACT 
     1441 TTGACCTGAA GCCGGAAAAC ATAATGCTTC TGGATAGAAA TGTGCCCAAA CCTCGGATCA 
     1501 AGATCATCGA CTTCGGCCTG GCCCATAAAA TTGACTTTGG AAATGAATTC AAAAACATAT 
     1561 TTGGGACACC AGAGTTTGTG GCTCCGGAGA TCGTCAACTA TGAGCCCCTT GGTCTTGAGG 
     1621 CAGACATGTG GAGCATTGGG GTAATAACCT ATATCCTCCT AAGCGGGGCC TCCCCTTTTC 
     1681 TTGGAGACAC CAAGCAAGAA ACATTAGCGA ATGTATCTGC TGTCAACTAT GACTTTGAGG 
     1741 AGGAATTCTT CCGCAACACC AGTACCCTTG CCAAAGATTT CATCAGAAGG CTGCTGGTCA 
     1801 AGGATCCAAA GAAGAGGATG ACAATCCAGG ACAGTTTGCA GCACCCCTGG ATCAAGCCTA 
     1861 AAGACACCCA ACAAGCACTT AGTCGAAAAG CCTCAGCAGT AAACATGGAG AAATTCAAGA 
     1921 AGTTTGCAGC TCGGAAAAAA TGGAAACAAT CTGTTCGCTT GATATCACTG TGCCAAAGAT 
     1981 TATCCAGGTC ATTTTTGTCC AGAAGTAACA TGAGCGTTGC CAGGAGCGAT GATACTCTGG 
     2041 ATGAGGAAGA CTCCTTTGTG ATGAAAGCCA TCATTCATGC CATCAATGAT GACAACGTCC 
     2101 CCGGCCTGCA GCACCTCCTG GGCTCATTGT CCAGCTATGA TGTCAACCAG CCCAACAAGC 
     2161 ACGGGACACC TCCATTACTG ATTGCTGCAG GCTGTGGGAA TATTCAGATG TTACAGTTAC 
     2221 TCATAAAACG GGGCTCAAGG ATCGACGTCC AGGATAAGGG AGGATCCAAT GCTATCTACT 
     2281 GGGCCTCTCG GCATGGCCAT GTGGATACTT TGAAGTTTCT CAATGAGAAC AAATGCCCTT 
     2341 TGGATGTTAA AGACAAGTCT GGAGAGACAG CTCTTCACGT GGCAGCCCGC TATGGCCATG 
     2401 CAGATGTGGT TCAACTCTTG TGCAGTTTTG GCTCTAATCC TGATTTCCAG GACAAGGAAG 
     2461 AGGAAACCCC CCTGCACTGT GCTGCCTGGC ACGGCTATTA CTCCGTGGCT AGAGCTCTTT 
     2521 GTGAAGTTGG CTGTAACGTG AATATCAAGA ACCGGGAGGG AGAGACCCCA CTGCTGACAG 
     2581 CTTCTGCCAG GGGCTACCAT GACATTGTGG AGTGTCTGGC TGAACATGGA GCTGACCTGA 
     2641 ATGCTTCTGA CAAGGATGGG CACATCGCCC TTCATCTTGC TGTGAGGCGC TGTCAGATGG 
     2701 AGGTCATCAA GACCCTCCTT GGCCATGGGT GCCTTGTGGA TTTCCAGGAT CGGCATGGCA 
     2761 ACACCCCCCT GCATGTGGCC TGCAAAGATG GGAGCGCGCC CATCGTGGTG GCCCTCTGTG 
     2821 AAGCCAGCTG CAACCTGGAC ATCTCAAACA AGTATGGTCG GACTCCTCTC CACCTTGCAG 
     2881 CCAACAACGG GATCCTAGAT GTGGTCCGCT ACCTCTGCCT GATGGGCGCC AACGTGGAGG 
     2941 CTCTGACCTT GGATGGCAAG ACGGCCGAGG ACCTCGCCAA GGCAGAGCAG CACGAGCACG 
     3001 TGGCAGGGCT CCTGGCAAGA CTGCGGAAGG ACACACACCG AGGGCTCTTC ATCCAGCAGC 
     3061 TCCGACCCAC GCAGAATCTC CAGCCCAGAA TCAAGCTCAA ACTGTTTGGC CACTCGGGAT 
     3121 CGGGGAAATC CACACTGGTG GAATCTCTCA AGTGTGGGCT GTTAAGGAGT TTCTTCAGAA 
     3181 GGCGCCGGCC CAGACTGTCC TCTACCAACT CCACCCGCTT CCCACCGTCG CCCCTGGCTA 
     3241 CTAAGCCAAC AGTCTCAGTG AGCATTAACA ACCTGTACCC CGGCTGTGAG AATGTGAGCG 
     3301 TGAGGAGCCG AAGCATGATG TTTGAGCCAG GCCTCACCAA AGGGATGCTG GAAGTGTTCG 
     3361 TGGCTCCGTC TCACCACCTA CACTGCTCGA CCGATGACCA GTCCACCAAA GCCATCGACA 
     3421 TCCAGAATGC TTACTTGAAC GGAGTTGGTG ATTTCAGCGT ATGGGAGTTC TCTGGAAACC 
     3481 CTGTGTACTT CTGTTGCTAT GACTACTTCG CCGCCAACGA TCCCACATCC ATCCACATCA 
     3541 TCGTTTTCAG TCTCGAAGAA CCCTATGAGA TCCAGCTGAA CCAAGTGATT TTCTGGCTTA 
     3601 GTTTCTTGAA GTCTCTGGTC TCAGTTGAAG AACCCATAGC ATTTGGAGGC AAGCTGAAGA 
     3661 ACCCTCTCCG AGTTGTCCTG GTGGCCACAC ATGCTGACAT CATGAACATC CCTCGGCCTG 
     3721 CTGGGGGCGA GTTTGGATAT GATAAAGATA CATCCTTGCT GAAAGAGATC AGGAACAGGT 
     3781 TTGGGAATGA CCTTCATGTC TCAAATAAGC TGTTTGTGCT GGATGCAGGA GCATCTGGGT 
     3841 CTAAGGACAT CAAGGTTCTC CGGAATCACC TGCAAGAAAT ACGGAGTCAG ATTGTCTCGG 
     3901 GGTGCCCTCC CATGACTCAT CTGTGTGAGA AGATCATCTC CACTCTGCCC TCCTGGCGGA 
     3961 AGCTCAACGG GCCAAACCAG CTGATGTCGC TCCAGCAGTT TGTGTATGAC GTGCAGGACC 
     4021 AGCTGAACCC CCTGGCCTCC GAGGATGACC TCAGGCGCAT TGCACAGCAG CTGCACAGCA 
     4081 CGGGAGAGAT CAACATCATG CAGAGTGAGA CAGTGCAGGA TGTGTTGCTG CTGGATCCTC 
     4141 GATGGCTCTG CACCAACGTC CTGGGGAAGC TGCTGTCTGT GGAGACTCCC CGAGCCCTGC 
     4201 ACCATTATCG GGGCCGCTAC ACCATGGAAG ATATCCAGCG TCTGGTCCCC GACAGTGACG 
     4261 TGGAGGAGCT GCTGCAGATC CTGGATGCCA TGGATATCTG CGCCCGAGAC CTGAGTAGCG 
     4321 GGACTATGGT GGATATCCCT GCTCTAATCA AAACAGACAG CCTGCAGCGC TCCTGGGCCG 
     4381 ATGAGGAAGA CGAGGTGATG GTGTACGGAG GTGTGCGCAT CGTCCCTGTA GAGCACCTCA 
     4441 CTCCCTTCCC CTGTGGCATC TTTCACAAAG TTCAGGTCAA CCTGTGCCGG TGGATCCACC 
     4501 AGCAGAGCAC TGAGGGGGAC GCGGACATCC GTCTGTGGGT GAGCGGCTGC AGGATCGCCA 
     4561 ACCGTGGGGC TGAGTTGCTG GTGCTGCTGG TCAATCACGG TCAGGGCATT GAGGTACAGG 
     4621 TGCGTGGACT GGAGACCGAG AAGATTAAGT GCTGCCTGCT GCTGGACTCG GTGTGCAGCA 
     4681 CCATCGAGAC TGTCATGGCC ACCACCTTGC CAGGGCTGTT GACGGTGAAG CACTACCTGA 
     4741 GCCCCCAGCA GCTAAGGGAG CATCACGAAC CAGTCATGGT CTACCAGCCC CGGGACTTCT 
     4801 TCCGTGCGCA GACCCTGAAA GAGAGCTCCC TCACCAACAC CATGGGAGGG TACAAGGAGA 
     4861 GCTTCAGCAG CATCACGTGC TTTGGGTGTC ACGATGTCTA CTCACAGGCC AGTCTTGGCA 
     4921 TGGACATCCA TGCATCAGAC CTGAGTCTCC TGACCCGGAG GAAACTGAGT CGTCTTCTTG 
     4981 ACCCACCGGA CCCCATGGGG AAGGACTGGT GCCTTCTGGC CATGAACTTG GGCCTCCCGG 
     5041 ACATGGTGGC CAAACACAAC GTCAATACCA GGGCTTCTAG GGATTTCCTC CCCAGCCCAG 
     5101 TGCATGCCTT GCTACAGGAA TGGACCTCCT ACCCTGAGAG CACGGTGGGC ATCCTTATAT 
     5161 CCAAACTTCG GGAGCTGGGG CGCCGGGATG CTGCGGACTT TTTACTGAAG GCCTCCTCTG 
     5221 TGTTCAAGAT CAACCTTGAC GGCAATGGCC AGGAGGCCTA TGCCTCAAGC TGTAACAGTG 
     5281 GCACATCCTA CAATTCCATA AGCTCAGTGG TGTCCCGGAC GCGTACGCGG CCGCTCGAGC 
     5341 AGAAACTCAT CTCAGAAGAG GATCTGGCAG CAAATGATAT CCTGGATTAC AAGGATGACG 
     5401 ACGATAAGGT TTAAACGGCC GGCCGCGGTC ATAGCTGTTT CCTGAACAGA TCCCGGGTGG 
     5461 CATCCCTGTG ACCCCTCCCC AGTGCCTCTC CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC 
     5521 ACCAGCCTTG TCCTAATAAA ATTAAGTTGC ATCATTTTGT CTGACTAGGT GTCCTTCTAT 
     5581 AATATTATGG GGTGGAGGGG GGTGGTATGG AGCAAGGGGC AAGTTGGGAA GACAACCTGT 
     5641 AGGGCCTGCG GGGTCTATTG GGAACCAAGC TGGAGTGCAG TGGCACAATC TTGGCTCACT 
     5701 GCAATCTCCG CCTCCTGGGT TCAAGCGATT CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA 
     5761 TTCCAGGCAT GCATGACCAG GCTCAGCTAA TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC 
     5821 ACCATATTGG CCAGGCTGGT CTCCAACTCC TAATCTCAGG TGATCTACCC ACCTTGGCCT 
     5881 CCCAAATTGC TGGGATTACA GGCGTGAACC ACTGCTCCCT TCCCTGTCCT TCTGATTTTA 
     5941 AAATAACTAT ACCAGCAGGA GGACGTCCAG ACACAGCATA GGCTACCTGG CCATGCCCAA 
     6001 CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC ACTGTCCTCT CATGCGTTGG GTCCACTCAG 
     6061 TAGATGCCTG TTGAATTGGG TACGCGGCCA GCGGCGAGCG GTATCAGCTC ACTCAAAGGC 
     6121 GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG 
     6181 CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG 
     6241 CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG 
     6301 ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC 
     6361 CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA 
     6421 TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT 
     6481 GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC 
     6541 CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG 
     6601 AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC 
     6661 TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT 
     6721 TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA 
     6781 GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG 
     6841 GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA 
     6901 AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT 
     6961 ATATGAGTAA CCTGAGGCTA TGGCAGGGCC TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG 
     7021 GCCTTCACCC GAACTTGGGG GGTGGGGTGG GGAAAAGGAA GAAACGCGGG CGTATTGGCC 
     7081 CCAATGGGGT CTCGGTGGGG TATCGACAGA GTGCCAGCCC TGGGACCGAA CCCCGCGTTT 
     7141 ATGAACAAAC GACCCAACAC CGTGCGTTTT ATTCTGTCTT TTTATTGCCG TCATAGCGCG 
     7201 GGTTCCTTCC GGTATTGTCT CCTTCCGTGT TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA 
     7261 GAACTCCAGC ATGAGATCCC CGCGCTGGAG GATCATCCAG CCGGCGTCCC GGAAAACGAT 
     7321 TCCGAAGCCC AACCTTTCAT AGAAGGCGGC GGTGGAATCG AAATCTCGTG ATGGCAGGTT 
     7381 GGGCGTCGCT TGGTCGGTCA TTTCGAACCC CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA 
     7441 AGGCGATAGA AGGCGATGCG CTGCGAATCG GGAGCGGCGA TACCGTAAAG CACGAGGAAG 
     7501 CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA GCAATATCAC GGGTAGCCAA CGCTATGTCC 
     7561 TGATAGCGAT CCGCCACACC CAGCCGGCCA CAGTCGATGA ATCCAGAAAA GCGGCCATTT 
     7621 TCCACCATGA TATTCGGCAA GCAGGCATCG CCATGGGTCA CGACGAGATC CTCGCCGTCG 
     7681 GGCATGCTCG CCTTGAGCCT GGCGAACAGT TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG 
     7741 TCCAGATCAT CCTGATCGAC AAGACCGGCT TCCATCCGAG TACGTGCTCG CTCGATGCGA 
     7801 TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA GCCGGATCAA GCGTATGCAG CCGCCGCATT 
     7861 GCATCAGCCA TGATGGATAC TTTCTCGGCA GGAGCAAGGT GAGATGACAG GAGATCCTGC 
     7921 CCCGGCACTT CGCCCAATAG CAGCCAGTCC CTTCCCGCTT CAGTGACAAC GTCGAGCACA 
     7981 GCTGCGCAAG GAACGCCCGT CGTGGCCAGC CACGATAGCC GCGCTGCCTC GTCTTGCAGT 
     8041 TCATTCAGGG CACCGGACAG GTCGGTCTTG ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC 
     8101 AGCCGGAACA CGGCGGCATC AGAGCAGCCG ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT 
     8161 AGCCTCTCCA CCCAAGCGGC CGGAGAACCT GCGTGCAATC CATCTTGTTC AATCATGCGA 
     8221 AACGATCCTC ATCCTGTCTC TTGATCGATC TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC 
     8281 TCCTCACTAC TTCTGGAATA GCTCAGAGGC CGAGGCGGCC TCGGCCTCTG CATAAATAAA 
     8341 AAAAATTAGT CAGCCATGGG GCGGAGAATG GGCGGAACTG GGCGGAGTTA GGGGCGGGAT 
     8401 GGGCGGAGTT AGGGGCGGGA CTATGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     8461 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCTGGTT GCTGACTAAT TGAGATGCAT 
     8521 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCCTA ACTGACACAC 
     8581 ATTCCACAGC TGGTTCTTTC CGCCTCAGGA CTCTTCCTTT TTCAATATTA TTGAAGCATT 
     8641 TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA 
     8701 ATAGGGGTTC CGCGCACATT TCCCCGAAAA GTGCCACCTG ACGCGCCCTG TAGCGGCGCA 
     8761 TTAAGCGCGG CGGGTGTGGT GGTTACGCGC AGCGTGACCG CTACACTTGC CAGCGCCCTA 
     8821 GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT 
     8881 CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG TTCCGATTTA GTGCTTTACG GCACCTCGAC 
     8941 CCCAAAAAAC TTGATTAGGG TGATGGTTCA CGTAGTGGGC CATCGCCCTG ATAGACGGTT 
     9001 TTTCGCCCTT TGACGTTGGA GTCCACGTTC TTTAATAGTG GACTCTTGTT CCAAACTGGA 
     9061 ACAACACTCA ACCCTATCTC GGTCTATTCT TTTGATTTAT AAGGGATTTT GCCGATTTCG 
     9121 GCCTATTGGT TAAAAAATGA GCTGATTTAA CAAAAATTTA ACGCGAATTT T
//