LOCUS       RR215273                8778 bp    DNA     circular  SYN 
DEFINITION  Rad54l2 (Myc-DDK-tagged ORF) - Rat Rad54 like 2 (S. cerevisiae) (Rad54l2), (10 ug)
ACCESSION   NM_001134520
VERSION     NM_001134520.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4935..5026 
                     /label="Myc-DDK"  
     misc_feature    5056..5641 
                     /label="PolyA signal"  
     misc_feature    5113..5132 
                     /label="Restriction Enzyme"  
     misc_feature    5917..6589 
                     /label="ColE1"  
     misc_feature    7019..7813 
                     /label="Kan/Neo"  
     misc_feature    7848..8150 
                     /label="SV40 ori"  
     misc_feature    8236..8713 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4917..4923 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4917 
                     /label="Rad54l2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTCAGACGAA TCCGCCTCAG GGAGCGATCC AGACCTGGAC CCGGACGTGG 
     1081 AGCTGGAGGA TGCGGAAGAG GAGGAGGAGG AGGAGGAGGT GGCAGTGGAG GAGCATGACA 
     1141 GGGATGACGA GGAAGGCCTG CTGGATGACC CATCCCTGGA AGGCATGTGT GGCACTGAGC 
     1201 ATGCCCAGCT GGGGGAAGAT GGGCAGCGGC CGCCGCGGTG CACTTCAACT ACCTCATCTC 
     1261 AGTCTGAGCC TTCAGAGCAG CTTAGGCACC AAGGCAAGAT CTTAGCATCT GAGGACCCCA 
     1321 AAAAGAAGCG AGCTCAGAAG CCCTCTCACA TGAGAAGAAA CATACGAAAG CTACTTCGGG 
     1381 AGGATCAGTT GGAGCCCGTT ACCAAAGCAG CACAGCAGGA AGAATTGGAA AGAAGAAAGC 
     1441 GCCTGGAGCA GCAGAGGAAA GAATATGCAG CCCCTATTCC TACTGTCCCT TTGGAGTTCC 
     1501 TACCTGAGGA AATTGTCTTA CGAGCTAGTG ATGGTCCCCA GCTCCCTCCT CGGGTCTTGG 
     1561 CCCAGGAAGT CATTTGTTTG GACAGTAGCA GTGGCAGTGA GGATGAAAAA AGCAGTCGAG 
     1621 ACGAGGTCAT TGAACTGAGT TCTGGAGAGG AGGACACTCT GCACATTGTG GACAGCAGTG 
     1681 AGTCTGTCAG TGAGGAGGAC GAGGAAGAGG AGAAGGGTGG CACCCATGTG AATGACGCCT 
     1741 TAAACCAGCA TGACGCTCTT GGGCGGGTAC TTGTCAACCT GAATCACCCT CCAGAGGAGG 
     1801 AGAATGTCTT CCTGGCCCCA CAGTTGGCAC GGGCTGTGAA ACCTCATCAG ATTGGTGGGA 
     1861 TCCGGTTCCT ATATGATAAC CTAGTGGAGT CCTTAGAAAG GTTTAAGACC AGTAGTGGCT 
     1921 TTGGCTGTAT CCTGGCCCAT AGCATGGGTC TGGGGAAAAC TCTGCAAGTG ATCTCCTTCA 
     1981 TTGATGTCCT TTTCCGCCAC ACGCCAGCCA AAACAGTCCT TGCGATTGTA CCGGTGAACA 
     2041 CTCTTCAGAA TTGGTTGGCA GAGTTCAACA TGTGGCTCCC GGCTCCTGAA GCCCTCCCAG 
     2101 CCGACAGCAA GCCTGAAGAA ATCCAGCCTC GGTTCTTTAA AGTTCATATC TTGAATGATG 
     2161 AACACAAGAC AGTGGCATCC CGTGCTAAAG TGACGACTGA TTGGGTTTCA GAGGGTGGAG 
     2221 TGCTGCTGAT GGGGTATGAG ATGTACAGAC TGCTCACCCT GAAGAAGTCC TTAGCCACAG 
     2281 GCAGACCCAA GAAAACCAAG AAACGGTCTC ACCCGGTCAT CATTGATCTG GATGAAGAAG 
     2341 ATCGGCAGCA GGAGTTCCGG AGAGAGTTTG AGAAGGCCTT ATGTCGCCCT GGTCCTGATG 
     2401 TGGTAATCTG TGACGAGGGA CACCGCATCA AAAATTGCCA AGCCAGCACC TCACAGGCTC 
     2461 TGAAGAACAT ACGTTCTCGT CGGCGGGTAG TGCTGACCGG CTACCCTCTA CAGAACAACC 
     2521 TCATCGAGTA CTGGTGCATG GTGGACTTTG TGCGCCCAGA TTTCCTTGGT ACTCGTCAGG 
     2581 AGTTCAGCAA TATGTTTGAA CGCCCTATCC TAAACGGACA GTGTATTGAC AGCACACCTC 
     2641 AGGATGTCCG CCTCATGCGC TACCGGAGCC ATGTTTTGCA CAGCCTCCTA GAGGGCTTTG 
     2701 TGCAGAGGAG AGGCCACACT GTGTTGAAGA TTCACCTCCC TGCCAAAGAA GAGAATGTAA 
     2761 TCCTGGTGCG GCTTTCTCAG ATCCAGCGGG ATTTGTACAC ACAATTCATG GACCGTTTCC 
     2821 GGGATTGTGG TAGCAGTGGC TGGTTGGGGC TGAATCCTCT GAAGGCTTTC TGTGTATGCT 
     2881 GCAAGATCTG GAACCATCCT GATGTGCTAT ATGAAGCCCT TCAGAAGGAA AACCTAGCCA 
     2941 ATGAGCAGGA CCTAGATGTG GAAGAACTTG GCTCAGCAGG GACCAGTACC CGCTGTCCAC 
     3001 CACATGGCAC AAAAGTCAAG GGAGAAGATA GTGCCTTGCC TTCCTCAATG GGAGAAGCAA 
     3061 CCAATAGCAA GTTCCTACAG GGAGTTGGCT TTAATCCTTT CCAGGAACGG GGCAATAACA 
     3121 TCGTTACATA TGAATGGGCC AAGGATCTTC TGACTAATTA TCAGACTGGA GTCCTGGAAA 
     3181 ACTCTCCCAA GATGGTACTT CTTTTCCACC TGATTGAAGA GAGTGTGAAG CTTGGGGACA 
     3241 AGATTCTTGT ATTTAGCCAG AGCCTTTCTA CCCTGGCTCT CATCGAGGAG TTCCTAGGGA 
     3301 AACGAGACAT GCCTTGTCCG CCTGGTGCCG AGGGGCAAGG AGCACAGAAG TGGGTTCGAA 
     3361 ATGTCAGCTA CTTCCGGCTA GACGGTAGCA CCCCTGCCTT TGAGAGGGAG CGGCTCATTA 
     3421 ATCAGTTCAA TGATCCCAGC AACCTCACCA CCTGGCTGTT CCTTCTCTCT ACAAGGGCCG 
     3481 GATGCTTGGG TGTGAATCTG ATTGGTGCCA ATCGAGTGGT GGTATTTGAT GCTTCCTGGA 
     3541 ACCCTTGCCA TGATGCCCAG GCAGTATGTC GGGTATACCG TTATGGCCAG AAAAAGCCCT 
     3601 GTTACATCTA TCGACTCGTG GCTGATTATA CTCTTGAAAA GAAGATCTAT GACCGGCAGA 
     3661 TTTCCAAGCA GGGCATGTCA GATCGGGTCG TGGATGATCT AAATCCAATG CTGAACTTCA 
     3721 CCCGGAAGGA AGTGGAAAAC CTGCTGCATT TTGTTGAGAA GGAGCCAGCT CCCCAAACAT 
     3781 CTTTGGATAT AAAGGGGATC AAGGAGTCAG TCTTGCAACT TGCCTGCCTG AAGTATCCTC 
     3841 ACCTCATCAC CAAGGAGCCT TTTGAGCACG AGTCACTGCT CCTCAACCGA AAAGATCACA 
     3901 AGCTGACCAA AGCTGAGAAG AAAGCAGCAA AGAAAAGCTA TGAGGAGGAC AAACGCACAT 
     3961 CAGTACCCTA CACCCGCCCA TCGTATGCGC AGTATTATCC TGCCAGCGAC CAGAGCCTGA 
     4021 CCAGCATCCC TGCCTTCAGT CAGAGGAACT GGCAGCCAAC ACTGAAGGGT GATGATAAGC 
     4081 CTGTGGCCAG CGTTCGTCCT GTACAGTCCA CCCCCATCCC CATGATGCCC CGGCATGTCC 
     4141 CACTCGGTGG CAGTGTAAGC TCTGCCTCTA ACACAAATCC ATCCATGAAC TTCCCCATCA 
     4201 ACTACTTGCA GCGGGCAGGA GTCCTTGTGC AGAAAGTGGT CACCACGACA GATATTGTTA 
     4261 TTCCTGGACT CAACAGCTCG ACAGATGTTC AGGCAAGGAT CAGTGCTGGT GAGAGCATCC 
     4321 ACATCATCCG GGGGACGAAA GGGACATACA TCCGTACCAG TGATGGACGA ATCTTTGCTG 
     4381 TCCGGGCAAC TGGCAAACCA AAGGCCCCTG AAGATGGTCG GATGGCTGCC TCAGGTTCCC 
     4441 AGGGGCCTTC TCTTGCATCC ACGAGCAATG GCAGACACAG TGCCTCATCA CCCAAAGCCC 
     4501 CTGACCCTGA GGGGCTGGCC AGGCCCGTCT CTCCTGACAG CCCAGAAATC ATCAGTGAGC 
     4561 TCCAGCAGTA TGCAGATGTG GCCGCTGCTC GGGAATCCCA TCAGAACTCC CCAAGCATCA 
     4621 GTGCTGCCCT GCCTGGCCCC CCGGCCCAAC TTATGGACAA TAGCACCATT CCTGGGACAG 
     4681 CTCTTGGAAC TGAGCCATGC CTTGGGGGTC ATTGCCTCAA TAGTTCCCTC TTGGTGACTG 
     4741 GTCAGCCCAG TAGTGGCAGG CATCCAGTGC TGGACTTAAG GGGCCATAAG CGAAAGTTGG 
     4801 CCACTCCATC TGTCTCCCAG GAATCAGTCC GTCGACGGTC CAGGAAGGGC CATCTGCCAG 
     4861 CCCCGTGCAG CCCTATGAAC ACGGGTATCC AGTCTCTGGC GGGTTTTCAA TGCCACCTGT 
     4921 CTCCTACGCG TACGCGGCCG CTCGAGCAGA AACTCATCTC AGAAGAGGAT CTGGCAGCAA 
     4981 ATGATATCCT GGATTACAAG GATGACGACG ATAAGGTTTA AACGGCCGGC CGCGGTCATA 
     5041 GCTGTTTCCT GAACAGATCC CGGGTGGCAT CCCTGTGACC CCTCCCCAGT GCCTCTCCTG 
     5101 GCCCTGGAAG TTGCCACTCC AGTGCCCACC AGCCTTGTCC TAATAAAATT AAGTTGCATC 
     5161 ATTTTGTCTG ACTAGGTGTC CTTCTATAAT ATTATGGGGT GGAGGGGGGT GGTATGGAGC 
     5221 AAGGGGCAAG TTGGGAAGAC AACCTGTAGG GCCTGCGGGG TCTATTGGGA ACCAAGCTGG 
     5281 AGTGCAGTGG CACAATCTTG GCTCACTGCA ATCTCCGCCT CCTGGGTTCA AGCGATTCTC 
     5341 CTGCCTCAGC CTCCCGAGTT GTTGGGATTC CAGGCATGCA TGACCAGGCT CAGCTAATTT 
     5401 TTGTTTTTTT GGTAGAGACG GGGTTTCACC ATATTGGCCA GGCTGGTCTC CAACTCCTAA 
     5461 TCTCAGGTGA TCTACCCACC TTGGCCTCCC AAATTGCTGG GATTACAGGC GTGAACCACT 
     5521 GCTCCCTTCC CTGTCCTTCT GATTTTAAAA TAACTATACC AGCAGGAGGA CGTCCAGACA 
     5581 CAGCATAGGC TACCTGGCCA TGCCCAACCG GTGGGACATT TGAGTTGCTT GCTTGGCACT 
     5641 GTCCTCTCAT GCGTTGGGTC CACTCAGTAG ATGCCTGTTG AATTGGGTAC GCGGCCAGCG 
     5701 GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA 
     5761 CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC 
     5821 GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC 
     5881 AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG 
     5941 CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT 
     6001 CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA 
     6061 GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC 
     6121 CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC 
     6181 AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT 
     6241 GAAGTGGTGG CCTAACTACG GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT 
     6301 GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC 
     6361 TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA 
     6421 AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA 
     6481 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA 
     6541 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAACCT GAGGCTATGG CAGGGCCTGC 
     6601 CGCCCCGACG TTGGCTGCGA GCCCTGGGCC TTCACCCGAA CTTGGGGGGT GGGGTGGGGA 
     6661 AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA ATGGGGTCTC GGTGGGGTAT CGACAGAGTG 
     6721 CCAGCCCTGG GACCGAACCC CGCGTTTATG AACAAACGAC CCAACACCGT GCGTTTTATT 
     6781 CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC 
     6841 AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA CTCCAGCATG AGATCCCCGC GCTGGAGGAT 
     6901 CATCCAGCCG GCGTCCCGGA AAACGATTCC GAAGCCCAAC CTTTCATAGA AGGCGGCGGT 
     6961 GGAATCGAAA TCTCGTGATG GCAGGTTGGG CGTCGCTTGG TCGGTCATTT CGAACCCCAG 
     7021 AGTCCCGCTC AGAAGAACTC GTCAAGAAGG CGATAGAAGG CGATGCGCTG CGAATCGGGA 
     7081 GCGGCGATAC CGTAAAGCAC GAGGAAGCGG TCAGCCCATT CGCCGCCAAG CTCTTCAGCA 
     7141 ATATCACGGG TAGCCAACGC TATGTCCTGA TAGCGATCCG CCACACCCAG CCGGCCACAG 
     7201 TCGATGAATC CAGAAAAGCG GCCATTTTCC ACCATGATAT TCGGCAAGCA GGCATCGCCA 
     7261 TGGGTCACGA CGAGATCCTC GCCGTCGGGC ATGCTCGCCT TGAGCCTGGC GAACAGTTCG 
     7321 GCTGGCGCGA GCCCCTGATG CTCTTCGTCC AGATCATCCT GATCGACAAG ACCGGCTTCC 
     7381 ATCCGAGTAC GTGCTCGCTC GATGCGATGT TTCGCTTGGT GGTCGAATGG GCAGGTAGCC 
     7441 GGATCAAGCG TATGCAGCCG CCGCATTGCA TCAGCCATGA TGGATACTTT CTCGGCAGGA 
     7501 GCAAGGTGAG ATGACAGGAG ATCCTGCCCC GGCACTTCGC CCAATAGCAG CCAGTCCCTT 
     7561 CCCGCTTCAG TGACAACGTC GAGCACAGCT GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC 
     7621 GATAGCCGCG CTGCCTCGTC TTGCAGTTCA TTCAGGGCAC CGGACAGGTC GGTCTTGACA 
     7681 AAAAGAACCG GGCGCCCCTG CGCTGACAGC CGGAACACGG CGGCATCAGA GCAGCCGATT 
     7741 GTCTGTTGTG CCCAGTCATA GCCGAATAGC CTCTCCACCC AAGCGGCCGG AGAACCTGCG 
     7801 TGCAATCCAT CTTGTTCAAT CATGCGAAAC GATCCTCATC CTGTCTCTTG ATCGATCTTT 
     7861 GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC TCACTACTTC TGGAATAGCT CAGAGGCCGA 
     7921 GGCGGCCTCG GCCTCTGCAT AAATAAAAAA AATTAGTCAG CCATGGGGCG GAGAATGGGC 
     7981 GGAACTGGGC GGAGTTAGGG GCGGGATGGG CGGAGTTAGG GGCGGGACTA TGGTTGCTGA 
     8041 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     8101 CCTGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     8161 GGACTTTCCA CACCCTAACT GACACACATT CCACAGCTGG TTCTTTCCGC CTCAGGACTC 
     8221 TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA 
     8281 TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG 
     8341 CCACCTGACG CGCCCTGTAG CGGCGCATTA AGCGCGGCGG GTGTGGTGGT TACGCGCAGC 
     8401 GTGACCGCTA CACTTGCCAG CGCCCTAGCG CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT 
     8461 CTCGCCACGT TCGCCGGCTT TCCCCGTCAA GCTCTAAATC GGGGGCTCCC TTTAGGGTTC 
     8521 CGATTTAGTG CTTTACGGCA CCTCGACCCC AAAAAACTTG ATTAGGGTGA TGGTTCACGT 
     8581 AGTGGGCCAT CGCCCTGATA GACGGTTTTT CGCCCTTTGA CGTTGGAGTC CACGTTCTTT 
     8641 AATAGTGGAC TCTTGTTCCA AACTGGAACA ACACTCAACC CTATCTCGGT CTATTCTTTT 
     8701 GATTTATAAG GGATTTTGCC GATTTCGGCC TATTGGTTAA AAAATGAGCT GATTTAACAA 
     8761 AAATTTAACG CGAATTTT
//