LOCUS       RR215265                7188 bp    DNA     circular  SYN 
DEFINITION  Pigr (Myc-DDK-tagged ORF) - Rat polymeric immunoglobulin receptor (Pigr), (10 ug)
ACCESSION   NM_012723
VERSION     NM_012723.4
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3345..3436 
                     /label="Myc-DDK"  
     misc_feature    3466..4051 
                     /label="PolyA signal"  
     misc_feature    3523..3542 
                     /label="Restriction Enzyme"  
     misc_feature    4327..4999 
                     /label="ColE1"  
     misc_feature    5429..6223 
                     /label="Kan/Neo"  
     misc_feature    6258..6560 
                     /label="SV40 ori"  
     misc_feature    6646..7123 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3327..3333 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3327 
                     /label="Pigr"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAGGCTCTCC TTGTTCGCCC TCTTGGTAAC TGTCTTCTCA GGGGTCTCCA 
     1081 CACAAAGCCC CATATTTGGT CCCCAGGATG TGAGTAGTAT TGAAGGTAAC TCGGTCTCCA 
     1141 TCACGTGCTA CTACCCAGAC ACCTCTGTCA ACCGGCACAC CCGGAAATAC TGGTGCCGAC 
     1201 AAGGAGCCAA CGGCTACTGC GCAACCCTCA TCTCTTCAAA TGGCTACCTC TCGAAGGAGT 
     1261 ATTCAGGCAG AGCCAGCCTC ATCAACTTCC CAGAGAATAG CACATTTGTG ATTAACATTG 
     1321 CACATCTCAC CCAGGAGGAC ACTGGGAGCT ACAAGTGTGG TCTGGGTACC ACTAACCGAG 
     1381 GCCTGTTTTT CGATGTCAGC CTGGAGGTCA GCCAGGTTCC TGAGTTCCCA AATGACACCC 
     1441 ATGTCTACAC AAAGGACATA GGCAGAACTG TGACCATCGA ATGCCGTTTC AAAGAGGGGA 
     1501 ATGCTCATAG CAAGAAATCC CTGTGTAAGA AGAGAGGAGA GGCCTGCGAA GTTGTCATCG 
     1561 ACTCTACTGA GTACGTGGAC CCCAGCTATA AGGACAGAGC AATCCTTTTT ATGAAAGGGA 
     1621 CCAGCCGCGA TATATTCTAT GTCAACATTA GCCACCTAAT ACCCAGTGAT GCTGGACTGT 
     1681 ATGTTTGCCA AGCTGGAGAA GGCCCCAGTG CTGATAAAAA TAATGCTGAC CTCCAGGTGC 
     1741 TAGAGCCTGA GCCAGAGCTG CTTTATAAAG ACCTGAGGTC CTCAGTGACT TTTGAATGTG 
     1801 ACCTGGGCCG TGAAGTGGCA AATGATGCCA AATATCTGTG TCGGAAGAAC AAGGAAACCT 
     1861 GTGATGTCAT CATCAACACC CTGGGGAAGA GAGATCCAGC CTTTGAAGGC AGGATCCTGC 
     1921 TAACCCCCAG GGATGACAAT GGCCGCTTCA GTGTGTTGAT CACAGGCCTG AGGAAGGAGG 
     1981 ATGCAGGGCA CTACCAGTGT GGAGCGCACA GTTCTGGTTT GCCTCAAGAA GGCTGGCCCG 
     2041 TCCAGGCTTG GCAACTCTTT GTCAATGAAG AGTCCACGAT TCCCAATAGT CGCTCTGTTG 
     2101 TGAAGGGTGT CACAGGAGGC TCTGTGGCCA TCGTCTGTCC CTATAACCCC AAGGAAAGCA 
     2161 GCAGCCTCAA GTACTGGTGT CACTGGGAAG CCGACGAGAA TGGACGCTGC CCGGTGCTCG 
     2221 TGGGGACCCA GGCCCTGGTG CAAGAAGGAT ATGAAGGCCG ACTGGCACTG TTCGATCAGC 
     2281 CGGGCAGTGG CGCCTACACT GTCATCCTCA ACCAGCTCAC CACCCAGGAT TCTGGCTTCT 
     2341 ACTGGTGTCT TACCGATGGT GACTCTCGCT GGAGAACCAC GATAGAACTG CAGGTTGCTG 
     2401 AAGCTACAAA GAAGCCAGAC CTTGAGGTGA CACCACAGAA CGCGACCGCG GTGATAGGAG 
     2461 AGACCTTCAC AATCTCCTGC CACTATCCGT GCAAATTCTA CTCCCAGGAG AAATACTGGT 
     2521 GCAAGTGGAG CAACGACGGC TGCCACATCC TGCCGAGCCA TGATGAAGGT GCCCGCCAGT 
     2581 CCTCTGTGAG CTGTGACCAG AGCAGCCAGA TCGTCTCCAT GACCCTGAAC CCGGTCAAAA 
     2641 AGGAAGATGA AGGCTGGTAC TGGTGTGGGG TAAAAGAAGG TCAGGTCTAT GGAGAAACTA 
     2701 CAGCCATCTA TGTAGCAGTT GAAGAGAGGA CCAGAGGGTC ACCCCACATC AACCCGACAG 
     2761 ATGCAAACGC ACGTGCAAAA GATGCTCCAG AGGAAGAGGC AATGGAATCC TCTGTCAGGG 
     2821 AGGATGAAAA CAAGGCCAAT CTGGACCCCA GGCTTTTTGC AGACGAAAGA GAGATACAGA 
     2881 ATGCGGGAGA CCAAGCTCAG GAGAACAGAG CATCTGGGAA TGCTGGCAGT GCTGGTGGAC 
     2941 AAAGCGGGAG CTCCAAAGTC CTATTCTCCA CCCTGGTGCC CCTGGGTTTG GTGCTGGCAG 
     3001 TGGGTGCTGT GGCTGTGTGG GTGGCCAGAG TCCGACATCG GAAGAATGTA GACCGCATGT 
     3061 CAATCAGCAG CTACAGGACA GACATTAGCA TGGGAGACTT CAGGAACTCC AGGGATTTGG 
     3121 GAGGCAATGA CAACATGGGC GCCACTCCAG ACACACAAGA AACAGTCCTC GAAGGAAAAG 
     3181 ATGAAATAGA GACTACCACC GAGTGTACCA CCGAGCCAGA GGAATCCAAG AAAGCAAAAA 
     3241 GGTCATCCAA GGAGGAAGCT GACATGGCCT ACTCAGCATT CCTGTTTCAG TCCAGCACAA 
     3301 TAGCTGCGCA GGTCCATGAT GGTCCCCAGG AAGCCACGCG TACGCGGCCG CTCGAGCAGA 
     3361 AACTCATCTC AGAAGAGGAT CTGGCAGCAA ATGATATCCT GGATTACAAG GATGACGACG 
     3421 ATAAGGTTTA AACGGCCGGC CGCGGTCATA GCTGTTTCCT GAACAGATCC CGGGTGGCAT 
     3481 CCCTGTGACC CCTCCCCAGT GCCTCTCCTG GCCCTGGAAG TTGCCACTCC AGTGCCCACC 
     3541 AGCCTTGTCC TAATAAAATT AAGTTGCATC ATTTTGTCTG ACTAGGTGTC CTTCTATAAT 
     3601 ATTATGGGGT GGAGGGGGGT GGTATGGAGC AAGGGGCAAG TTGGGAAGAC AACCTGTAGG 
     3661 GCCTGCGGGG TCTATTGGGA ACCAAGCTGG AGTGCAGTGG CACAATCTTG GCTCACTGCA 
     3721 ATCTCCGCCT CCTGGGTTCA AGCGATTCTC CTGCCTCAGC CTCCCGAGTT GTTGGGATTC 
     3781 CAGGCATGCA TGACCAGGCT CAGCTAATTT TTGTTTTTTT GGTAGAGACG GGGTTTCACC 
     3841 ATATTGGCCA GGCTGGTCTC CAACTCCTAA TCTCAGGTGA TCTACCCACC TTGGCCTCCC 
     3901 AAATTGCTGG GATTACAGGC GTGAACCACT GCTCCCTTCC CTGTCCTTCT GATTTTAAAA 
     3961 TAACTATACC AGCAGGAGGA CGTCCAGACA CAGCATAGGC TACCTGGCCA TGCCCAACCG 
     4021 GTGGGACATT TGAGTTGCTT GCTTGGCACT GTCCTCTCAT GCGTTGGGTC CACTCAGTAG 
     4081 ATGCCTGTTG AATTGGGTAC GCGGCCAGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT 
     4141 AATACGGTTA TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA 
     4201 GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC 
     4261 CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT 
     4321 ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT 
     4381 GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG 
     4441 CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA 
     4501 CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA 
     4561 CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC 
     4621 GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG 
     4681 AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG 
     4741 TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA 
     4801 GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC 
     4861 TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG 
     4921 GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA 
     4981 TGAGTAACCT GAGGCTATGG CAGGGCCTGC CGCCCCGACG TTGGCTGCGA GCCCTGGGCC 
     5041 TTCACCCGAA CTTGGGGGGT GGGGTGGGGA AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA 
     5101 ATGGGGTCTC GGTGGGGTAT CGACAGAGTG CCAGCCCTGG GACCGAACCC CGCGTTTATG 
     5161 AACAAACGAC CCAACACCGT GCGTTTTATT CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT 
     5221 TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA 
     5281 CTCCAGCATG AGATCCCCGC GCTGGAGGAT CATCCAGCCG GCGTCCCGGA AAACGATTCC 
     5341 GAAGCCCAAC CTTTCATAGA AGGCGGCGGT GGAATCGAAA TCTCGTGATG GCAGGTTGGG 
     5401 CGTCGCTTGG TCGGTCATTT CGAACCCCAG AGTCCCGCTC AGAAGAACTC GTCAAGAAGG 
     5461 CGATAGAAGG CGATGCGCTG CGAATCGGGA GCGGCGATAC CGTAAAGCAC GAGGAAGCGG 
     5521 TCAGCCCATT CGCCGCCAAG CTCTTCAGCA ATATCACGGG TAGCCAACGC TATGTCCTGA 
     5581 TAGCGATCCG CCACACCCAG CCGGCCACAG TCGATGAATC CAGAAAAGCG GCCATTTTCC 
     5641 ACCATGATAT TCGGCAAGCA GGCATCGCCA TGGGTCACGA CGAGATCCTC GCCGTCGGGC 
     5701 ATGCTCGCCT TGAGCCTGGC GAACAGTTCG GCTGGCGCGA GCCCCTGATG CTCTTCGTCC 
     5761 AGATCATCCT GATCGACAAG ACCGGCTTCC ATCCGAGTAC GTGCTCGCTC GATGCGATGT 
     5821 TTCGCTTGGT GGTCGAATGG GCAGGTAGCC GGATCAAGCG TATGCAGCCG CCGCATTGCA 
     5881 TCAGCCATGA TGGATACTTT CTCGGCAGGA GCAAGGTGAG ATGACAGGAG ATCCTGCCCC 
     5941 GGCACTTCGC CCAATAGCAG CCAGTCCCTT CCCGCTTCAG TGACAACGTC GAGCACAGCT 
     6001 GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC GATAGCCGCG CTGCCTCGTC TTGCAGTTCA 
     6061 TTCAGGGCAC CGGACAGGTC GGTCTTGACA AAAAGAACCG GGCGCCCCTG CGCTGACAGC 
     6121 CGGAACACGG CGGCATCAGA GCAGCCGATT GTCTGTTGTG CCCAGTCATA GCCGAATAGC 
     6181 CTCTCCACCC AAGCGGCCGG AGAACCTGCG TGCAATCCAT CTTGTTCAAT CATGCGAAAC 
     6241 GATCCTCATC CTGTCTCTTG ATCGATCTTT GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC 
     6301 TCACTACTTC TGGAATAGCT CAGAGGCCGA GGCGGCCTCG GCCTCTGCAT AAATAAAAAA 
     6361 AATTAGTCAG CCATGGGGCG GAGAATGGGC GGAACTGGGC GGAGTTAGGG GCGGGATGGG 
     6421 CGGAGTTAGG GGCGGGACTA TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     6481 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCTGGTTGCT GACTAATTGA GATGCATGCT 
     6541 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCCTAACT GACACACATT 
     6601 CCACAGCTGG TTCTTTCCGC CTCAGGACTC TTCCTTTTTC AATATTATTG AAGCATTTAT 
     6661 CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA 
     6721 GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTGACG CGCCCTGTAG CGGCGCATTA 
     6781 AGCGCGGCGG GTGTGGTGGT TACGCGCAGC GTGACCGCTA CACTTGCCAG CGCCCTAGCG 
     6841 CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT CTCGCCACGT TCGCCGGCTT TCCCCGTCAA 
     6901 GCTCTAAATC GGGGGCTCCC TTTAGGGTTC CGATTTAGTG CTTTACGGCA CCTCGACCCC 
     6961 AAAAAACTTG ATTAGGGTGA TGGTTCACGT AGTGGGCCAT CGCCCTGATA GACGGTTTTT 
     7021 CGCCCTTTGA CGTTGGAGTC CACGTTCTTT AATAGTGGAC TCTTGTTCCA AACTGGAACA 
     7081 ACACTCAACC CTATCTCGGT CTATTCTTTT GATTTATAAG GGATTTTGCC GATTTCGGCC 
     7141 TATTGGTTAA AAAATGAGCT GATTTAACAA AAATTTAACG CGAATTTT
//