LOCUS       RR215048                8796 bp    DNA     circular  SYN 
DEFINITION  Gak (Myc-DDK-tagged ORF) - Rat cyclin G associated kinase (Gak), (10 ug)
ACCESSION   NM_031030
VERSION     NM_031030.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4953..5044 
                     /label="Myc-DDK"  
     misc_feature    5074..5659 
                     /label="PolyA signal"  
     misc_feature    5131..5150 
                     /label="Restriction Enzyme"  
     misc_feature    5935..6607 
                     /label="ColE1"  
     misc_feature    7037..7831 
                     /label="Kan/Neo"  
     misc_feature    7866..8168 
                     /label="SV40 ori"  
     misc_feature    8254..8731 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4935..4941 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4935 
                     /label="Gak"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTCGCTGCTG CAGTCTGCAC TGGACTTCCT GGCGGGCCCT GGTTCTCTTG 
     1081 GCGGAGCTGC CGGCCGTGAC CAGAGTGACT TCGTGGGGCA GACTGTGGAG CTGGGCGAGC 
     1141 TGCGTCTGCG GGTGCGGCGG GTCCTGGCCG AGGGAGGGTT TGCATTTGTT TATGAAGCTC 
     1201 AAGATCTGGG AAGTGGCAGA GAGTATGCAT TAAAGAGATT ACTATCCAAT GAAGAGGAAA 
     1261 AGAACAGAGC CATCATTCAG GAAGTATGTT TCTTGAAAAA ACTTTCTGGC CACCCCAATA 
     1321 TTGTCCAGTT CTGCTCTGCA GCATCCATAG GAAAAGAGGA ATCGGACACT GGGCAGGCTG 
     1381 AGTTCCTCCT GCTTACGGAG CTCTGTAAAG GACAGCTGGT GGAGTTTCTC AGGAGAGTTG 
     1441 AATGTAAAGG CCCTCTATCC TGCGACAGCA TTCTGAAGAT CTTCTACCAG ACATGCAGAG 
     1501 CAGTGCAGCA CATGCACAGG CAGAAACCAC CCATCATCCA CAGGGATCTC AAGGTTGAAA 
     1561 ACTTACTGCT TAGTAACCAG GGGACCATTA AGCTGTGTGA CTTTGGCAGT GCCACAACCA 
     1621 TCTCCCATTA TCCTGACTAC AGCTGGAGCG CCCAGAAGCG AGCAATGGTG GAGGAAGAGA 
     1681 TCACGAGGAA CACCACACCC ATGTACAGAA CGCCAGAAAT TGTAGACCTG TATTCTAACT 
     1741 TCCCTATTGG CGAAAAGCAG GATATCTGGG CACTGGGCTG TATCTTATAC CTGCTGTGTT 
     1801 TCCGGCAGCA TCCTTTTGAA GATGGAGCAA AACTTCGGAT AGTCAATGGG AAGTATTCCA 
     1861 TTCCTGTGAA TGACACTCGT TACACAGTCT TCCATGACCT TATTCGTGGC ATGCTAAAGG 
     1921 TCAATCCAGA AGAGAGGCTA TCCATTGCTG AAGTTGTCCG ACAACTGCAG GAAATTGCAG 
     1981 CAGCCCGGAA TGTGAACCCC AAAGCCCCCA TCACAGAACT TCTGGAGCAG AATGGTGGCT 
     2041 ATGGGAACTC AGGGCCTTCC CGAGCACAAC CACCTTCTGG GGGCCCTGTG AACAGCAGTG 
     2101 GAGTTTTGGC TCTGGCAGAG TATGACCAGC CCTATGGTGG GTTTCTCGAT ATCCTACGGG 
     2161 GTGGGACAGA ACGGCTCTTC ACCAACCTCA AGGATACTTC CTCCAAGGTC ATCCAGTCTG 
     2221 TGGCTAACTA TGCAAAGGGC GATCTTGACA TATCTTACAT CACATCCAGG ATTGCAGTGA 
     2281 TGTCGTTCCC AGCAGAAGGT GTGGAGTCAG CAATCAAAAA CAATATAGAG GATGTACGAT 
     2341 TGTTTCTGGA TGCCAAGCAT CCAGGACATT ATGCTGTCTA CAACCTTTCT CCAAGAATAT 
     2401 ACCGGGCTTC CAAGTTCCAC AATCGGGTCA CTGAGTGTGG CTGGGCAGTC AGGCGGGCAC 
     2461 CACATCTCCA CAGTTTGTAT ACTCTATGCA GGAGCATGCA TGCCTGGCTC CGGGAAGACC 
     2521 ACAGGAACGT CTGTGTTGTA CATTGCATGG ATGGGAGAGC TGCGTCTGCT GTGGCAGTCT 
     2581 GTGCATTCCT GTGCTTCTGC CGTCTCTTCA GCACTGCAGA GGCTGCTGTG TACATGTTCA 
     2641 GCATGAAGCG CTGCCCACCA GGCATTTGGC CATCCCACAA AAGGTACATT GAATACGTGT 
     2701 GTGACATGGT GGCAGAGGAA CCCATCACGC CCCACAGCAA GCCAATGCTG GTGAAATCTG 
     2761 TTGTCATGAC CCCTGTGCCA CTGTTCAGCA AGCAGAGGAA TGGCTGCCGA CCATTCTGTG 
     2821 AGGTCTATGT TGGGGAGGAG CGTGTAACCA CCACATCCCA GGAGTATGAC AGGATGAAGG 
     2881 AATTTAAAAT TGAGGATGGC AAGGCTGTCA TCCCCCTGGG CATAACAGTT CAAGGCGATG 
     2941 TCCTCACCAT CATTTACCAT GCCAGATCCA CTTTAGGAGG GAGACTGCAG GCCAAGATGG 
     3001 CGTCCATGAA AATGTTCCAG ATTCAGTTCC ACACTGGGTT CGTGCCTCGA AACGCAACCA 
     3061 CTGTGAAATT TGCAAAGTAT GACCTGGATG CTTGTGACAT TCAAGAGAAG TACCCAGATC 
     3121 TGTTCCAGGT GAACCTGGAA GTGGAGGTGG AGCCTAGAGA CAGGCCCAGC CGAGACGTTC 
     3181 CACCTTGGGA GAATACCAGC CTAAGGGGGT TAAACCCCAA GATCCTCTTT TCCAACAGGG 
     3241 AAGAGCAACA GGACATTCTG TCTAAGTTTG GGAAACCAGA GCTACCCCGG CAGCCGGGCT 
     3301 CCACAGCTCA GTATGATGCT GAGGCAGGGT CTCCAGAGGC TGAGATCACA GAGTCGGACT 
     3361 CACCACAGAG CAGCAGCACG GACACCAACC ACTTTCTTCA CACACTGGAT TGGCAGGAGG 
     3421 AAAAAGACCC AGAGACTGGT GTAGACAATA CCTCTCCTAA GGAGAGTCAG TCTAACCTGA 
     3481 TTGCAGATGG AGATGGAAGT GAAGTATCAG ATGAAGAAGA GGCTTCATGC CCTAGTGAAG 
     3541 AGAGAAAGCC TGGGGCTGGA GAAGATACAC CAAGGCTGGC AGCTGGGACC AGACAGCAAG 
     3601 ACTTAATATT TGATGTGGGC ATGCTGGCTG CCCCACAGGA GCCTGTACAG CCTGAAGAAG 
     3661 GTGTCGATCT CCTGGGGCTG CACTCTGAAG GGGACTTAAG GCCTGCTGCT CCCTTGCAGG 
     3721 CTAGCGGGGT CCAGTCTAGC AACACTGACC TGTTGAGCTC CCTTCTTGAA CCATCTGATG 
     3781 CTTCTCAAGT GGGACCTCCT GGTGACCTGC TTGGTGGTGA GACTCCTCTG CTGTTAGCAA 
     3841 GCCCAGTTTC TCTTCTTGGG GTGCAGAGCA ACCTGCAAGG AAAAGTCCCT GACACTGTGG 
     3901 ACCCATTTGA CCAGTTCCTG CTGCCATCCA GCTCGGACAC CCAGCCCTGC TCCAAGCCTG 
     3961 ATCTCTTTGG AGAGTTTCTC AACTCTGACT CTGTAGCTTC CTCAACTGCC TTCCCATCGA 
     4021 CCCACAGCGC CCCACCCCCA TCCTGCAGCA CTGCCTTCCT GCACCTGGGA GATCTGCCAG 
     4081 CAGAGCCCAA CAAGGTGATT GCTTCATCCA GCCACCCAGA TCTGCTAGGA GGATGGGATA 
     4141 CGTGGGCTGA GACTGCTCTA CCTGGGCCGG CCTCCATGCC AGTACCAGAA GGTACTCTCT 
     4201 TCTCTTCTGC AGGTCACCCA GCCCCTCCAG GCCCCAACCC CAGCCAAACC AAGTCTCAGA 
     4261 ACCCAGACCC ATTTGCTGAC CTCAGTGATC TCAGCTCTAG CCTCCAAGGC TTGCCTGCTG 
     4321 GACTTCCTGC AGGGAGCTTC GTTGGCACAT CAGCCACCAC TCACAAAAGC AACAGCTCCT 
     4381 GGCAGACAAC TCGTCCCACA GCCCCTGGAA CCTCATGGCC CCCGCAGGCC AAGCCAGCCC 
     4441 CCAGAGCCTC TGAACAACTA AGGTCTCACT TTAGTGTGAT TGGGGCCCGA GAAGAGAGAG 
     4501 GTGTCCGTGC GCCCAGCTTT GCCCAAAAGC CAAAGGTCTC AGAAAATGAT TTTGAAGATC 
     4561 TGCTGCCTAA TCAAGGCTTC TCTAAGTCTG ACAAGAAGGG GCCAAAGACC ATGGCAGAGA 
     4621 TGCGGAAACA GGAACTTGCC AGAGATACAG ACCCATTCAA ATTGAAGCTT TTGGACTGGA 
     4681 TTGAAGGCAA AGAGAGGAAT ATTCGTGCAC TGCTGTCCAC TCTGCACACA GTATTGTGGG 
     4741 ATGGGGAGAG CCGCTGGACA CCTGTGAGTA TGGCTGACCT GGTGACTCCA GAGCAGGTGA 
     4801 AGAAGCAGTA CCGCCGTGCA GTGCTGGTAG TGCATCCTGA TAAGGCCACA GGGCAGCCAT 
     4861 ATGAACAGTC TGCCAAGATG ATCTTCATGG AGCTGAATGA TGCATGGTCT GAGTTTGAAA 
     4921 ACCAGGGCTC AAGGCCCCTC TTCACGCGTA CGCGGCCGCT CGAGCAGAAA CTCATCTCAG 
     4981 AAGAGGATCT GGCAGCAAAT GATATCCTGG ATTACAAGGA TGACGACGAT AAGGTTTAAA 
     5041 CGGCCGGCCG CGGTCATAGC TGTTTCCTGA ACAGATCCCG GGTGGCATCC CTGTGACCCC 
     5101 TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT GCCACTCCAG TGCCCACCAG CCTTGTCCTA 
     5161 ATAAAATTAA GTTGCATCAT TTTGTCTGAC TAGGTGTCCT TCTATAATAT TATGGGGTGG 
     5221 AGGGGGGTGG TATGGAGCAA GGGGCAAGTT GGGAAGACAA CCTGTAGGGC CTGCGGGGTC 
     5281 TATTGGGAAC CAAGCTGGAG TGCAGTGGCA CAATCTTGGC TCACTGCAAT CTCCGCCTCC 
     5341 TGGGTTCAAG CGATTCTCCT GCCTCAGCCT CCCGAGTTGT TGGGATTCCA GGCATGCATG 
     5401 ACCAGGCTCA GCTAATTTTT GTTTTTTTGG TAGAGACGGG GTTTCACCAT ATTGGCCAGG 
     5461 CTGGTCTCCA ACTCCTAATC TCAGGTGATC TACCCACCTT GGCCTCCCAA ATTGCTGGGA 
     5521 TTACAGGCGT GAACCACTGC TCCCTTCCCT GTCCTTCTGA TTTTAAAATA ACTATACCAG 
     5581 CAGGAGGACG TCCAGACACA GCATAGGCTA CCTGGCCATG CCCAACCGGT GGGACATTTG 
     5641 AGTTGCTTGC TTGGCACTGT CCTCTCATGC GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA 
     5701 TTGGGTACGC GGCCAGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC 
     5761 CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG 
     5821 GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA 
     5881 TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA 
     5941 GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG 
     6001 ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG 
     6061 GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT 
     6121 TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA 
     6181 CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG 
     6241 CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT 
     6301 TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC 
     6361 CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG 
     6421 CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG 
     6481 GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA 
     6541 GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAACCTGA 
     6601 GGCTATGGCA GGGCCTGCCG CCCCGACGTT GGCTGCGAGC CCTGGGCCTT CACCCGAACT 
     6661 TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG 
     6721 TGGGGTATCG ACAGAGTGCC AGCCCTGGGA CCGAACCCCG CGTTTATGAA CAAACGACCC 
     6781 AACACCGTGC GTTTTATTCT GTCTTTTTAT TGCCGTCATA GCGCGGGTTC CTTCCGGTAT 
     6841 TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC CCTAGGGTGG GCGAAGAACT CCAGCATGAG 
     6901 ATCCCCGCGC TGGAGGATCA TCCAGCCGGC GTCCCGGAAA ACGATTCCGA AGCCCAACCT 
     6961 TTCATAGAAG GCGGCGGTGG AATCGAAATC TCGTGATGGC AGGTTGGGCG TCGCTTGGTC 
     7021 GGTCATTTCG AACCCCAGAG TCCCGCTCAG AAGAACTCGT CAAGAAGGCG ATAGAAGGCG 
     7081 ATGCGCTGCG AATCGGGAGC GGCGATACCG TAAAGCACGA GGAAGCGGTC AGCCCATTCG 
     7141 CCGCCAAGCT CTTCAGCAAT ATCACGGGTA GCCAACGCTA TGTCCTGATA GCGATCCGCC 
     7201 ACACCCAGCC GGCCACAGTC GATGAATCCA GAAAAGCGGC CATTTTCCAC CATGATATTC 
     7261 GGCAAGCAGG CATCGCCATG GGTCACGACG AGATCCTCGC CGTCGGGCAT GCTCGCCTTG 
     7321 AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC CCCTGATGCT CTTCGTCCAG ATCATCCTGA 
     7381 TCGACAAGAC CGGCTTCCAT CCGAGTACGT GCTCGCTCGA TGCGATGTTT CGCTTGGTGG 
     7441 TCGAATGGGC AGGTAGCCGG ATCAAGCGTA TGCAGCCGCC GCATTGCATC AGCCATGATG 
     7501 GATACTTTCT CGGCAGGAGC AAGGTGAGAT GACAGGAGAT CCTGCCCCGG CACTTCGCCC 
     7561 AATAGCAGCC AGTCCCTTCC CGCTTCAGTG ACAACGTCGA GCACAGCTGC GCAAGGAACG 
     7621 CCCGTCGTGG CCAGCCACGA TAGCCGCGCT GCCTCGTCTT GCAGTTCATT CAGGGCACCG 
     7681 GACAGGTCGG TCTTGACAAA AAGAACCGGG CGCCCCTGCG CTGACAGCCG GAACACGGCG 
     7741 GCATCAGAGC AGCCGATTGT CTGTTGTGCC CAGTCATAGC CGAATAGCCT CTCCACCCAA 
     7801 GCGGCCGGAG AACCTGCGTG CAATCCATCT TGTTCAATCA TGCGAAACGA TCCTCATCCT 
     7861 GTCTCTTGAT CGATCTTTGC AAAAGCCTAG GCCTCCAAAA AAGCCTCCTC ACTACTTCTG 
     7921 GAATAGCTCA GAGGCCGAGG CGGCCTCGGC CTCTGCATAA ATAAAAAAAA TTAGTCAGCC 
     7981 ATGGGGCGGA GAATGGGCGG AACTGGGCGG AGTTAGGGGC GGGATGGGCG GAGTTAGGGG 
     8041 CGGGACTATG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     8101 GCCTGGGGAC TTTCCACACC TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     8161 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCCTAACTGA CACACATTCC ACAGCTGGTT 
     8221 CTTTCCGCCT CAGGACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT 
     8281 CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC 
     8341 ACATTTCCCC GAAAAGTGCC ACCTGACGCG CCCTGTAGCG GCGCATTAAG CGCGGCGGGT 
     8401 GTGGTGGTTA CGCGCAGCGT GACCGCTACA CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC 
     8461 GCTTTCTTCC CTTCCTTTCT CGCCACGTTC GCCGGCTTTC CCCGTCAAGC TCTAAATCGG 
     8521 GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT TTACGGCACC TCGACCCCAA AAAACTTGAT 
     8581 TAGGGTGATG GTTCACGTAG TGGGCCATCG CCCTGATAGA CGGTTTTTCG CCCTTTGACG 
     8641 TTGGAGTCCA CGTTCTTTAA TAGTGGACTC TTGTTCCAAA CTGGAACAAC ACTCAACCCT 
     8701 ATCTCGGTCT ATTCTTTTGA TTTATAAGGG ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA 
     8761 AATGAGCTGA TTTAACAAAA ATTTAACGCG AATTTT
//