LOCUS       RR214977                8589 bp    DNA     circular  SYN 
DEFINITION  Plcb3 (Myc-DDK-tagged ORF) - Rat phospholipase C, beta 3 (phosphatidylinositol-specific) (Plcb3), (10 ug)
ACCESSION   NM_033350
VERSION     NM_033350.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4746..4837 
                     /label="Myc-DDK"  
     misc_feature    4867..5452 
                     /label="PolyA signal"  
     misc_feature    4924..4943 
                     /label="Restriction Enzyme"  
     misc_feature    5728..6400 
                     /label="ColE1"  
     misc_feature    6830..7624 
                     /label="Kan/Neo"  
     misc_feature    7659..7961 
                     /label="SV40 ori"  
     misc_feature    8047..8524 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4728..4734 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4728 
                     /label="Plcb3"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGGGCGCG AGGCCCGGCG TCCACGCGCT GCAGCTGGAG CCGCCCACCG 
     1081 TGGTGGAGAC CCTGCGGCGC GGGAGTAAAT TCATCAAATG GGACGAGGAG GCCTCCAGTC 
     1141 GGAACCTGGT GACCCTGCGA GTGGACCCCA ATGGCTTCTT CTTGTACTGG ACAGGACCCA 
     1201 ACATGGAGGT GGACACACTG GACATCAGCT CCATCAGGGA CACAAGGACA GGTCGTTATG 
     1261 CCCGCCTGCC CAAGGACCCC AAGATTCGAG AGGTACTGGG CTTTGGGGGT CCTGACACCC 
     1321 GGCTGGAGGA GAAACTGATG ACAGTGGTAG CAGGACCAGA TCCAGTAAAC ACCACATTCT 
     1381 TGAACTTCAT GGCCGTGCAA GATGACACAG TCAAGGTCTG GTCAGAGGAG TTGTTTAAGC 
     1441 TGGCTATGAA CATACTGGCT CAGAACGCCT CCCGGAACAC GTTCCTTCGG AAAGCATACA 
     1501 CGAAACTGAA GCTGCAGGTG AACCAGGATG GACGGATTCC AGTCAAGAAC ATTCTGAAGA 
     1561 TGTTCTCCGC GGACAAGAAG CGGGTGGAGA CTGCACTGGA GTCCTGCGGC CTCAACTTCA 
     1621 ACCGGAGTGA GTCCATTCGG CCTGATGAGT TTTCCTTGGA AATCTTCGAG CGGTTCTTGA 
     1681 ATAAGCTGTG TCTGCGACCG GATATTGACA AGATCCTGCT GGAGATAGGT GCTAAGGGCA 
     1741 AGCCGTACCT CACTCTGGAG CAGCTCATGG ACTTTATCAA CCAGAAGCAA AGAGACCCAA 
     1801 GACTCAACGA GGTGCTGTAC CCACCGCTTC GCTCCTCACA GGCTCGGCTG CTCATTGAGA 
     1861 AGTATGAGCC GAACAAACAG TTCCTGGAGC GCGACCAGAT GTCTATGGAG GGTTTCAGCC 
     1921 GCTATCTGGG AGGGGAGGAG AATGGCATCC TGCCCCTGGA GGCCCTGGAT CTGAGCATGG 
     1981 ACATGACGCA GCCACTGAGC GCATACTTCA TCAATTCTTC ACACAATACC TATCTCACTG 
     2041 CGGGCCAGCT GGCTGGAACA TCGTCAGTGG AGATGTACCG CCAGGCGCTG CTGTGGGGCT 
     2101 GCCGTTGTGT GGAGCTGGAT GTATGGAAGG GACGGCCACC GGAGGAAGAG CCCTTCATTA 
     2161 CCCATGGTTT CACCATGACC ACTGAGGTGC CATTGCGTGA TGTGTTAGAG GCCATCGCCG 
     2221 AGACAGCCTT CAAGACCTCA CCCTACCCTG TCATTCTCTC CTTCGAAAAC CATGTGGACT 
     2281 CGGCAAAACA GCAGGCCAAG ATGGCAGAGT ACTGCCGCTC TATCTTTGGG GAAGCGCTGC 
     2341 TCATTGACCC ACTGGACAAG TACCCGCTGT CTGCGGGCAC CCCCCTTCCT AGTCCACAGG 
     2401 ACCTGATGGG CCGTATCCTG GTGAAGAATA AGAAGCGGCA TCGGCCCAGC ACAGGTGTCC 
     2461 CTGACAGCTC CGTGCGCAAG CGGCCTCTGG AACAGAGCAA TTCAGCCCTG AGTGAGAGCT 
     2521 CAGCTGCCAC AGAGCCCTCC TCACCCCAGC TGGGGTCCCC CAGCTCTGAC AGCTGCCCTG 
     2581 GCCTAAGCAA TGGGGAAGAG GTGGGACTTG AGAAGACCAG CCTGGAGCCT CAGAAGTCTC 
     2641 TAGGTGAGGA GGGCCTCAAC CGAGGACCTA ATGTTCTCAT GCCTGACCGT GACCGAGAGG 
     2701 ATGAGGAGGA AGATGAAGAA GAGGAGGAAA CAACGGATCC AAAAAAGCCC ACCACCGATG 
     2761 AGGGCACAGC CAGCAGTGAG GTCAACGCCA CAGAGGAGAT GTCGACACTC GTCAATTACG 
     2821 TTGAGCCCGT CAAGTTCAAG TCCTTTGAGG CTGCTCGAAA AAGGAACAAG TGCTTTGAGA 
     2881 TGTCGTCCTT TGTGGAGACC AAGGCGATGG AGCAGCTGAC CAAGAGCCCC ATGGAGTTCG 
     2941 TGGAATACAA CAAACAGCAG CTTAGTCGCA TCTACCCCAA GGGCACCCGT GTGGACTCCT 
     3001 CCAACTACAT GCCTCAGCTC TTCTGGAACG TGGGCTGTCA GCTTGTTGCC CTCAACTTCC 
     3061 AGACCTTGGA TTTGCCAATG CAGCTCAACG CAGGCGTGTT TGAGTACAAC GGGCGCAGTG 
     3121 GGTACTTGCT CAAGCCTGAG TTTATGCGGC GGCCGGACAA GTCTTTTGAC CCCTTCACTG 
     3181 AGGTCATCGT AGATGGCATA GTGGCCAACG CCTTGCGGGT CAAGGTGATT TCGGGGCAGT 
     3241 TTCTGTCTGA CAGGAAGGTG GGCATCTATG TCGAGGTGGA CATGTTTGGT CTCCCAGTTG 
     3301 ACACAAGACG CAAATATCGC ACACGGACAT CCCAGGGGAA CTCATTCAAC CCTGTGTGGG 
     3361 ATGAAGAACC CTTTGACTTC CCCAAGGTTG TGCTGCCCAC GCTGGCCTCA CTTCGCATTG 
     3421 CAGCCTTTGA GGAGGGTGGC AGATTTGTTG GGCATCGTAT CCTGCCTGTC TCTGCTATCC 
     3481 GCTCAGGATA CCACTACGTC TGCCTGCGAA ATGAGGCCAA CCAACCGTTG TGCCTGCCTG 
     3541 CCCTGCTTAT CTACACTGAA GCTTCTGACT ACATCCCAGA TGACCACCAG GACTATGCGG 
     3601 AGGCCTTGAT CAACCCCATC AAGCACGTCA GCCTAATGGA CCAGCGGGCC AAGCAACTGG 
     3661 CTGCTCTCAT TGGGGAGAGT GAGGCTCAGG CCAGTACAGA AATGTGTCAG GAGACCCCGT 
     3721 CTCAACAGCA AGGCTCACAG CTCTCCTCCA ACCCCGCACC TAACCCACTG GATGCCTCAC 
     3781 CTCGATGGCC CCCAGGTCCT ACCACCTCCC CCACTAGCAC CTCCCTCAGT AGCCCAGGGC 
     3841 AGCGAGATGA TCTGATCGCC AGCATCCTTT CTGAGGTGAC CCCAACACCC CTAGAGGAGC 
     3901 TCCGAAGCCA CAAGGCTATG GTGAAGCTCC GGAGCCGGCA GGATCGAGAC CTGAGGGAAC 
     3961 TGCATAAAAA ACACCAGCGC AAGGCTGTGG CCCTCACCCG CCGCCTTCTG GATGGCCTGG 
     4021 CCCAGGCCAG GGCTGAGGGG AAGTGCCGGC CCTCCTCCAG CGCCCTGAGC AGGGCCACCA 
     4081 ATGTGGAAGA CGTGAAGGAG GAAGAAAAAG AGGCAGCCAG ACAGTATCGA GAGTTCCAGA 
     4141 ACCGACAAGT ACAGAGCCTG CTGGAGCTGA GGGAGGCCCA GGCAGATGCA GAGACAGAAC 
     4201 GGAGGCTGGA GCATCTGAAA CAGGCTCAGC AGCGGCTCAG GGAGGTTGTC CTGGACGCAC 
     4261 ATACGACTCA GTTCAAGAGG CTGAAGGAGT TGAATGAAAG GGAGAAGAAG GAGCTTCAGA 
     4321 AGATCCTGGA CAGGAAGCGC AACAACAGCA TCTCAGAGGC CAAGACAAGG GAGAAACACA 
     4381 AGAAGGAGGT GGAACTGACA GAGATCAATC GGAGGCACAT CACTGAGTCA GTGAACTCCA 
     4441 TCAGGAGGCT GGAAGAGGCC CAGAAGCAGC GCCACGAACG CCTCTTGGCA GGGCAACAGC 
     4501 AGGTCCTCCA GCAGCTAGTG GAAGAGGAGC CCAAGCTGGT GGCCCAGCTG ACCCAGGAGT 
     4561 GTCAGGAACA GCGAGAGAGG CTGCCCCAGG AGATCCGTCG GTGCCTGCTG GGCGAGACAT 
     4621 CAGAGGGATT GGGGGATGGC CCCCTGGTGG CCTGTGCCAG CAATGGTCAT GCAGCTGGGA 
     4681 GTGGTGGGCA CCAGTCCGGC GCTGACTCGG AGAGCCAGGA GGAAAACACC CAGCTTACGC 
     4741 GTACGCGGCC GCTCGAGCAG AAACTCATCT CAGAAGAGGA TCTGGCAGCA AATGATATCC 
     4801 TGGATTACAA GGATGACGAC GATAAGGTTT AAACGGCCGG CCGCGGTCAT AGCTGTTTCC 
     4861 TGAACAGATC CCGGGTGGCA TCCCTGTGAC CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA 
     4921 GTTGCCACTC CAGTGCCCAC CAGCCTTGTC CTAATAAAAT TAAGTTGCAT CATTTTGTCT 
     4981 GACTAGGTGT CCTTCTATAA TATTATGGGG TGGAGGGGGG TGGTATGGAG CAAGGGGCAA 
     5041 GTTGGGAAGA CAACCTGTAG GGCCTGCGGG GTCTATTGGG AACCAAGCTG GAGTGCAGTG 
     5101 GCACAATCTT GGCTCACTGC AATCTCCGCC TCCTGGGTTC AAGCGATTCT CCTGCCTCAG 
     5161 CCTCCCGAGT TGTTGGGATT CCAGGCATGC ATGACCAGGC TCAGCTAATT TTTGTTTTTT 
     5221 TGGTAGAGAC GGGGTTTCAC CATATTGGCC AGGCTGGTCT CCAACTCCTA ATCTCAGGTG 
     5281 ATCTACCCAC CTTGGCCTCC CAAATTGCTG GGATTACAGG CGTGAACCAC TGCTCCCTTC 
     5341 CCTGTCCTTC TGATTTTAAA ATAACTATAC CAGCAGGAGG ACGTCCAGAC ACAGCATAGG 
     5401 CTACCTGGCC ATGCCCAACC GGTGGGACAT TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA 
     5461 TGCGTTGGGT CCACTCAGTA GATGCCTGTT GAATTGGGTA CGCGGCCAGC GGCGAGCGGT 
     5521 ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA 
     5581 GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC 
     5641 GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG 
     5701 GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT 
     5761 GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG 
     5821 AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG 
     5881 CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG 
     5941 TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC 
     6001 TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG 
     6061 GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT 
     6121 TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG 
     6181 TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC 
     6241 TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT 
     6301 GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT 
     6361 TAAATCAATC TAAAGTATAT ATGAGTAACC TGAGGCTATG GCAGGGCCTG CCGCCCCGAC 
     6421 GTTGGCTGCG AGCCCTGGGC CTTCACCCGA ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA 
     6481 AACGCGGGCG TATTGGCCCC AATGGGGTCT CGGTGGGGTA TCGACAGAGT GCCAGCCCTG 
     6541 GGACCGAACC CCGCGTTTAT GAACAAACGA CCCAACACCG TGCGTTTTAT TCTGTCTTTT 
     6601 TATTGCCGTC ATAGCGCGGG TTCCTTCCGG TATTGTCTCC TTCCGTGTTT CAGTTAGCCT 
     6661 CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT GAGATCCCCG CGCTGGAGGA TCATCCAGCC 
     6721 GGCGTCCCGG AAAACGATTC CGAAGCCCAA CCTTTCATAG AAGGCGGCGG TGGAATCGAA 
     6781 ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG GTCGGTCATT TCGAACCCCA GAGTCCCGCT 
     6841 CAGAAGAACT CGTCAAGAAG GCGATAGAAG GCGATGCGCT GCGAATCGGG AGCGGCGATA 
     6901 CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT TCGCCGCCAA GCTCTTCAGC AATATCACGG 
     6961 GTAGCCAACG CTATGTCCTG ATAGCGATCC GCCACACCCA GCCGGCCACA GTCGATGAAT 
     7021 CCAGAAAAGC GGCCATTTTC CACCATGATA TTCGGCAAGC AGGCATCGCC ATGGGTCACG 
     7081 ACGAGATCCT CGCCGTCGGG CATGCTCGCC TTGAGCCTGG CGAACAGTTC GGCTGGCGCG 
     7141 AGCCCCTGAT GCTCTTCGTC CAGATCATCC TGATCGACAA GACCGGCTTC CATCCGAGTA 
     7201 CGTGCTCGCT CGATGCGATG TTTCGCTTGG TGGTCGAATG GGCAGGTAGC CGGATCAAGC 
     7261 GTATGCAGCC GCCGCATTGC ATCAGCCATG ATGGATACTT TCTCGGCAGG AGCAAGGTGA 
     7321 GATGACAGGA GATCCTGCCC CGGCACTTCG CCCAATAGCA GCCAGTCCCT TCCCGCTTCA 
     7381 GTGACAACGT CGAGCACAGC TGCGCAAGGA ACGCCCGTCG TGGCCAGCCA CGATAGCCGC 
     7441 GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA CCGGACAGGT CGGTCTTGAC AAAAAGAACC 
     7501 GGGCGCCCCT GCGCTGACAG CCGGAACACG GCGGCATCAG AGCAGCCGAT TGTCTGTTGT 
     7561 GCCCAGTCAT AGCCGAATAG CCTCTCCACC CAAGCGGCCG GAGAACCTGC GTGCAATCCA 
     7621 TCTTGTTCAA TCATGCGAAA CGATCCTCAT CCTGTCTCTT GATCGATCTT TGCAAAAGCC 
     7681 TAGGCCTCCA AAAAAGCCTC CTCACTACTT CTGGAATAGC TCAGAGGCCG AGGCGGCCTC 
     7741 GGCCTCTGCA TAAATAAAAA AAATTAGTCA GCCATGGGGC GGAGAATGGG CGGAACTGGG 
     7801 CGGAGTTAGG GGCGGGATGG GCGGAGTTAG GGGCGGGACT ATGGTTGCTG ACTAATTGAG 
     7861 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCTGGTTGC 
     7921 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     7981 ACACCCTAAC TGACACACAT TCCACAGCTG GTTCTTTCCG CCTCAGGACT CTTCCTTTTT 
     8041 CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT 
     8101 ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC 
     8161 GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG TTACGCGCAG CGTGACCGCT 
     8221 ACACTTGCCA GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT TCTCGCCACG 
     8281 TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC CTTTAGGGTT CCGATTTAGT 
     8341 GCTTTACGGC ACCTCGACCC CAAAAAACTT GATTAGGGTG ATGGTTCACG TAGTGGGCCA 
     8401 TCGCCCTGAT AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA 
     8461 CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG TCTATTCTTT TGATTTATAA 
     8521 GGGATTTTGC CGATTTCGGC CTATTGGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC 
     8581 GCGAATTTT
//