LOCUS       RR214960                7410 bp    DNA     circular  SYN 
DEFINITION  Fbxo11 (Myc-DDK-tagged ORF) - Rat F-box protein 11 (Fbxo11), (10 ug)
ACCESSION   NM_181631
VERSION     NM_181631.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3567..3658 
                     /label="Myc-DDK"  
     misc_feature    3688..4273 
                     /label="PolyA signal"  
     misc_feature    3745..3764 
                     /label="Restriction Enzyme"  
     misc_feature    4549..5221 
                     /label="ColE1"  
     misc_feature    5651..6445 
                     /label="Kan/Neo"  
     misc_feature    6480..6782 
                     /label="SV40 ori"  
     misc_feature    6868..7345 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3549..3555 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3549 
                     /label="Fbxo11"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGTTGCAGAA GAATCAGGTC CTGGTGCACA AAACAGTCCA TACCAGCTTC 
     1081 GTAGAAAAAC TCTTTTGCCA AAAAGAACAG CGTGTCCTAC AAAGAACAGC ATGGAGGGCG 
     1141 CCTCTACTTC AACTACAGAA AACTTCGGCC ATCGAGCGAA GCGGGCAAGA GTGTCTGGGA 
     1201 AGTCACAAGA TCTGTCAGCA GCACCCGCTG AACAGTATCT TCAGGAGAAG CTACCAGATG 
     1261 AAGTAGTTTT AAAAATATTC TCTTATTTGC TGGAACAAGA TCTTTGTAGA GCTGCTTGTG 
     1321 TGTGTAAACG CTTCAGTGAA CTTGCTAATG ATCCAATTTT GTGGAAACGA TTATATATGG 
     1381 AAGTATTTGA ATATACCCGG CCTATGATGC ATCCTGAACC TGGTAAATTC TACCAGATTA 
     1441 ATCCAGAAGA ATATGAACAT CCAAATCCAT GGAAAGAGAG TTTTCAACAG TTGTATAAAG 
     1501 GTGCACACGT AAAGCCGGGA TTTGCGGAAC ATTTCTATAG TAATCCTGCC AGATACAAAG 
     1561 GGAGAGAAAA CATGTTGTAT TATGATACTA TTGAAGATGC CCTTGGCGGA GTACAAGAAG 
     1621 CACATTTTGA TGGGCTTATC TTTGTTCATT CTGGAATATA TACTGATGAA TGGATATATA 
     1681 TTGAATCTCC AATCACTATG ATTGGTGCAG CACCTGGGAA GGTTGCAGAC AAGGTCATCA 
     1741 TTGAGAACAC TAGAGACTCC ACTTTCGTTT TTATGGAAGG TTCTGAGGAC GCTTACGTTG 
     1801 GATATATGAC AATAAGATTC AACCCTGATG ACAAATCTGC CCAGCATCAC AACGCGCACC 
     1861 ACTGCCTAGA GATCACAGTC AACTGCAGCC CCATTATCGA CCACTGTATC ATCCGAAGCA 
     1921 CATGCACAGT TGGCTCTGCA GTGTGTGTCA GCGGTCAGGG GGCGTGTCCC ACTATCAAGC 
     1981 ATTGTAACAT CAGTGACTGT GAAAACGTCG GCCTCTATAT AACAGATCAT GCACAGGGAA 
     2041 TATATGAAGA TAATGAAATT TCTAATAATG CATTAGCTGG GATTTGGGTT AAAAATCATG 
     2101 GAAATCCAAT TATTAGACGG AATCACATTC ATCATGGACG TGATGTTGGT GTATTCACAT 
     2161 TTGATCATGG CATGGGCTAC TTTGAAAGTT GCAACATACA CAGAAACAGG ATAGCAGGCT 
     2221 TTGAGGTAAA AGCCTACGCT AATCCCACAG TGGTTCGATG TGAAATTCAC CATGGGCAGA 
     2281 CTGGAGGAAT ATATGTCCAT GAGAAAGGAA GAGGACAATT TATAGAGAAT AAAATCTATG 
     2341 CAAACAACTT CGCAGGCGTA TGGATTACCT CAAATAGTGA CCCAACAATA AGGGGGAATT 
     2401 CTATATTTAA TGGAAATCAA GGAGGAGTTT ACATCTTTGG TGATGGACGA GGCCTTATAG 
     2461 AAGGAAATGA CATTTATGGT AATGCGTTAG CAGGAATTCA GATTAGGACA AACAGTTGTC 
     2521 CTATTGTTCG ACATAACAAA ATTCACGATG GACAGCATGG TGGGATTTAT GTGCATGAAA 
     2581 AGGGACAAGG TGTAATAGAA GAAAATGAAG TTTACAGCAA CACCCTGGCT GGCGTCTGGG 
     2641 TGACAACTGG CAGCACGCCA GTACTAAGAA GAAATCGGAT ACACAGTGGC AAGCAGGTCG 
     2701 GTGTTTATTT CTATGACAAT GGACATGGTG TTCTAGAAGA TAACGATATC TACAATCATA 
     2761 TGTATTCAGG GGTTCAGATA AGGACTGGAA GCAACCCCAA AATTAGAAGA AACAAAATTT 
     2821 GGGGAGGACA AAATGGTGGA ATTCTAGTTT ATAATTCTGG TCTAGGCTGT ATAGAAGACA 
     2881 ATGAAATCTT CGACAACGCA ATGGCTGGCG TCTGGATTAA AACAGACAGC AATCCTACGC 
     2941 TGAGACGGAA TAAAATCCAC GATGGAAGGG ATGGCGGCAT CTGTATATTT AATGGGGGTC 
     3001 GAGGTCTCCT TGAAGAAAAT GATATTTTCA GGAACGCTCA AGCAGGTGTT CTTATCAGCA 
     3061 CCAACAGTCA TCCAGTCTTA AGAAAAAACA GAATATTCGA TGGATTTGCT GCAGGTATTG 
     3121 AAATTACAAA TCATGCAACT GCAACACTAG AAGGCAATCA GATTTTTAAC AACAGATTTG 
     3181 GAGGCTTATT TTTAGCATCT GGTGTTAACG TGACAATGAA AGATAACAAG ATAATGAACA 
     3241 ATCAAGATGC CATAGAAAAG GCTGTTAGTA GAGGACAATG TTTATATAAA ATATCAAGTT 
     3301 ATACCAGCTA TCCCATGCAT GACTTCTACA GATGTCATAC TTGTAACACC ACAGATCGAA 
     3361 ATGCCATATG TGTGAACTGC ATTAAGAAGT GCCATCAGGG ACATGATGTA GAGTTCATTA 
     3421 GACATGATAG GTTTTTCTGT GACTGTGGTG CTGGAACACT GTCCAATCCT TGTACATTAG 
     3481 CTGGTGAGCC AACACATGAT ACAGACACAC TATATGACTC TGCCCCACCT ATAGAGTCTA 
     3541 ATACGTTGCA GCACAACACG CGTACGCGGC CGCTCGAGCA GAAACTCATC TCAGAAGAGG 
     3601 ATCTGGCAGC AAATGATATC CTGGATTACA AGGATGACGA CGATAAGGTT TAAACGGCCG 
     3661 GCCGCGGTCA TAGCTGTTTC CTGAACAGAT CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA 
     3721 GTGCCTCTCC TGGCCCTGGA AGTTGCCACT CCAGTGCCCA CCAGCCTTGT CCTAATAAAA 
     3781 TTAAGTTGCA TCATTTTGTC TGACTAGGTG TCCTTCTATA ATATTATGGG GTGGAGGGGG 
     3841 GTGGTATGGA GCAAGGGGCA AGTTGGGAAG ACAACCTGTA GGGCCTGCGG GGTCTATTGG 
     3901 GAACCAAGCT GGAGTGCAGT GGCACAATCT TGGCTCACTG CAATCTCCGC CTCCTGGGTT 
     3961 CAAGCGATTC TCCTGCCTCA GCCTCCCGAG TTGTTGGGAT TCCAGGCATG CATGACCAGG 
     4021 CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA CGGGGTTTCA CCATATTGGC CAGGCTGGTC 
     4081 TCCAACTCCT AATCTCAGGT GATCTACCCA CCTTGGCCTC CCAAATTGCT GGGATTACAG 
     4141 GCGTGAACCA CTGCTCCCTT CCCTGTCCTT CTGATTTTAA AATAACTATA CCAGCAGGAG 
     4201 GACGTCCAGA CACAGCATAG GCTACCTGGC CATGCCCAAC CGGTGGGACA TTTGAGTTGC 
     4261 TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG TCCACTCAGT AGATGCCTGT TGAATTGGGT 
     4321 ACGCGGCCAG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA 
     4381 ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG 
     4441 TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA 
     4501 AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT 
     4561 TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT 
     4621 GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT 
     4681 CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC 
     4741 CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT 
     4801 ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC 
     4861 TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGAACAG TATTTGGTAT 
     4921 CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA 
     4981 ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA 
     5041 AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA 
     5101 AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT 
     5161 TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAC CTGAGGCTAT 
     5221 GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC GAGCCCTGGG CCTTCACCCG AACTTGGGGG 
     5281 GTGGGGTGGG GAAAAGGAAG AAACGCGGGC GTATTGGCCC CAATGGGGTC TCGGTGGGGT 
     5341 ATCGACAGAG TGCCAGCCCT GGGACCGAAC CCCGCGTTTA TGAACAAACG ACCCAACACC 
     5401 GTGCGTTTTA TTCTGTCTTT TTATTGCCGT CATAGCGCGG GTTCCTTCCG GTATTGTCTC 
     5461 CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG GTGGGCGAAG AACTCCAGCA TGAGATCCCC 
     5521 GCGCTGGAGG ATCATCCAGC CGGCGTCCCG GAAAACGATT CCGAAGCCCA ACCTTTCATA 
     5581 GAAGGCGGCG GTGGAATCGA AATCTCGTGA TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT 
     5641 TTCGAACCCC AGAGTCCCGC TCAGAAGAAC TCGTCAAGAA GGCGATAGAA GGCGATGCGC 
     5701 TGCGAATCGG GAGCGGCGAT ACCGTAAAGC ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA 
     5761 AGCTCTTCAG CAATATCACG GGTAGCCAAC GCTATGTCCT GATAGCGATC CGCCACACCC 
     5821 AGCCGGCCAC AGTCGATGAA TCCAGAAAAG CGGCCATTTT CCACCATGAT ATTCGGCAAG 
     5881 CAGGCATCGC CATGGGTCAC GACGAGATCC TCGCCGTCGG GCATGCTCGC CTTGAGCCTG 
     5941 GCGAACAGTT CGGCTGGCGC GAGCCCCTGA TGCTCTTCGT CCAGATCATC CTGATCGACA 
     6001 AGACCGGCTT CCATCCGAGT ACGTGCTCGC TCGATGCGAT GTTTCGCTTG GTGGTCGAAT 
     6061 GGGCAGGTAG CCGGATCAAG CGTATGCAGC CGCCGCATTG CATCAGCCAT GATGGATACT 
     6121 TTCTCGGCAG GAGCAAGGTG AGATGACAGG AGATCCTGCC CCGGCACTTC GCCCAATAGC 
     6181 AGCCAGTCCC TTCCCGCTTC AGTGACAACG TCGAGCACAG CTGCGCAAGG AACGCCCGTC 
     6241 GTGGCCAGCC ACGATAGCCG CGCTGCCTCG TCTTGCAGTT CATTCAGGGC ACCGGACAGG 
     6301 TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC TGCGCTGACA GCCGGAACAC GGCGGCATCA 
     6361 GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA TAGCCGAATA GCCTCTCCAC CCAAGCGGCC 
     6421 GGAGAACCTG CGTGCAATCC ATCTTGTTCA ATCATGCGAA ACGATCCTCA TCCTGTCTCT 
     6481 TGATCGATCT TTGCAAAAGC CTAGGCCTCC AAAAAAGCCT CCTCACTACT TCTGGAATAG 
     6541 CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC ATAAATAAAA AAAATTAGTC AGCCATGGGG 
     6601 CGGAGAATGG GCGGAACTGG GCGGAGTTAG GGGCGGGATG GGCGGAGTTA GGGGCGGGAC 
     6661 TATGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     6721 GGACTTTCCA CACCTGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     6781 TGGGGAGCCT GGGGACTTTC CACACCCTAA CTGACACACA TTCCACAGCT GGTTCTTTCC 
     6841 GCCTCAGGAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG 
     6901 AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT 
     6961 CCCCGAAAAG TGCCACCTGA CGCGCCCTGT AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG 
     7021 GTTACGCGCA GCGTGACCGC TACACTTGCC AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC 
     7081 TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC 
     7141 CCTTTAGGGT TCCGATTTAG TGCTTTACGG CACCTCGACC CCAAAAAACT TGATTAGGGT 
     7201 GATGGTTCAC GTAGTGGGCC ATCGCCCTGA TAGACGGTTT TTCGCCCTTT GACGTTGGAG 
     7261 TCCACGTTCT TTAATAGTGG ACTCTTGTTC CAAACTGGAA CAACACTCAA CCCTATCTCG 
     7321 GTCTATTCTT TTGATTTATA AGGGATTTTG CCGATTTCGG CCTATTGGTT AAAAAATGAG 
     7381 CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//