LOCUS       RR214947                9258 bp    DNA     circular  SYN 
DEFINITION  Rfx7 (Myc-DDK-tagged ORF) - Rat regulatory factor X, 7 (Rfx7), (10 ug)
ACCESSION   NM_001127490
VERSION     NM_001127490.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5415..5506 
                     /label="Myc-DDK"  
     misc_feature    5536..6121 
                     /label="PolyA signal"  
     misc_feature    5593..5612 
                     /label="Restriction Enzyme"  
     misc_feature    6397..7069 
                     /label="ColE1"  
     misc_feature    7499..8293 
                     /label="Kan/Neo"  
     misc_feature    8328..8630 
                     /label="SV40 ori"  
     misc_feature    8716..9193 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5397..5403 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5397 
                     /label="Rfx7"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCAGAGGAA CAACAACAGC CGCCACCACA GCAGCTCGAT GCCCATCAGC 
     1081 AGCTTCCCCT CAGCGCCCCC AACCCCGGGG TGGCTCTGCC AGCCCTTGTG CCCGGGCTGC 
     1141 CAGGGACAGA GGCCAGCGCT CTGCAACACA AGATCAAGAA CTCCATCTGC AAAACTGTAC 
     1201 AATCTAAAGT GGACTGCATT TTGCAAGAAG TTGAGAAGTT TACAGACCTA GAGAAACTCT 
     1261 ACCTCTACCT TCAACTGCCT TCTGGTCTCA GCAGTGCAGA GAAAAGTGAT CAGAATGCCA 
     1321 TGTCATCTAG CCGGGCACAG CAAATGCATG CCTTTTCCTG GATTCGAAAT ACCTTAGAGG 
     1381 AGCATCCTGA GACTTCACTC CCCAAACAAG AAGTCTATGA TGAATACAAG AGCTATTGTG 
     1441 ACAATCTTGG CTACCATCCA TTAAGTGCTG CTGACTTTGG AAAAATCATG AAGAACGTCT 
     1501 TTCCAAACAT GAAGGCACGC CGTTTGGGCA CTAGAGGCAA ATCTAAATAT TGCTACAGTG 
     1561 GACTAAGGAA AAAAGCTTTT GTTCATATGC CAACACTGCC CAACCTTGAC TTTCATAAAA 
     1621 CTGGAGATGG GTTGGAAGGA GTTGAACCAT CTGGGCAGCT TCAAAATATT GATGAGGAAG 
     1681 TTATCTCTTC TGCTTGCCGT CTTGTGTGTG AGTGGGCCCA GAAAGTGTTA AGCCAGCCAT 
     1741 TTGACACAGT CTTGGAATTA GCCCACTTCC TTGTAAAGAG TCATTATATA GGCACCAAGT 
     1801 CAATGGCAGC TCTGACTGTG ATGGCAGCAG CACCAGCAGG ACTTAAAGGA ATTTCCCAGC 
     1861 CTTCTGCTTT TATACCTACA GCTGAAAGTA ATTCCTTTCA ACCTCAAGTA AAGACTTTGT 
     1921 CATCTCCAAT TGATGCTAAA CAGCAGTTGC AACGGAAAAT TCAAAAAAAG CAGCAAGAAC 
     1981 AGAAACTACA GTCCCCATTA CCAGGAGAAT CCTCAGCAAA AAAAACAGAA GGCACTACAG 
     2041 CCAACGGAGT GGCTAATCTA CCCAATGGGA ACCCTGCGAT CCTTTCTCCC CAGCCCATTG 
     2101 GGATTGTGGT AGCAGCTGTC CCTAGTCCCA TTCCGGTCCA GCGGACGAGG CAGTTAGTAA 
     2161 CTTCACCCAG TCCGATGAAT TCTTCTGATG GCAAGGTTCT TCCCCTCAAC GTGCAGGTAG 
     2221 TCACTCAGCA CATGCAGTCT GTGAAACAGA CACCAAAGAC TCCTCAGAAC GTCCCAGCCA 
     2281 GTCCTGGTGG GGACCGTTCT GCCAGGCACC GCTACCCTCA GATCTTACCC AAGCCAGCAA 
     2341 ACACCAGTGC GCTCACCATC CGCTCTCCTA CGACTGTGCT GTTTACCAGC AGTCCCATCA 
     2401 AAACTGCTGT GGTACCTGCG TCACACATGA GTTCTCTAAA TGTGGTGAAA ATGACAACAA 
     2461 TATCCCTCAC ACCCAGTAAC AGTAATGCGC CTCTTAAACA TTCTGCCTCC GTCAGCAATG 
     2521 CTACTGGAAC AACAGAGGAC TCCAGGAGCA TCCCTCAGAT CAAGAATGGT TCTGTTGTTT 
     2581 CTCTCCAGTC CCCTGGGTCC AGGGCCAGCA GCACTGGGGG AACCTCTGCT GTGGAAGTCA 
     2641 AAATGGAACC AGAAGGTTCA TCAGATGAGC ATCCTCTGCA GTGCCAAGAG AACGCTGAGG 
     2701 GGACTAAAGC TCCTCTGGCC ACATCTAGTG CCCTTTGGGG ACAGAAAAGT AATACAGATG 
     2761 GAACAGTATC AAAACCTTCT AATGAAGGAG TCACAGAAGT GAAAACAACT AAGGTCTGTG 
     2821 ACCAGAGGGC CAAGTGTAAA AGTCGCTGTA ATGAAACTCT GCCAGGCATC TCAGCAGGCA 
     2881 ATAATCAAAG CACTGTTACT CTCTCAGTTG CTACTCAGAA CTTACCCTTC ACCAGCACCA 
     2941 GCTCACCATC TAATGGTGAC TCAGTAAATA AAGACCCTAA ATTATGCACT AAAAGTCCAA 
     3001 GAAAACGACT GTCTGCTACA CTGCAAGAGT CTCAGGTGCC TCCTGTAAAG AAACCAATTG 
     3061 TAGAACAGCT TTCTGCAGTT ACTACCGAAG GTCAGAAACC AGGCACTGTT AAAAAGGACC 
     3121 AAAAGGTTCC ACATTCAGGG AAAACAGAAA GCTCAACAGC AGGTGCTCAG CCTCCTAACA 
     3181 AGGTGTCACT CAGTGTCAGC TCACACATTG TAGAGGATCA GCCCTTGAAC CCTACTCTTG 
     3241 TCGCCAGTGA ATCAGCTCTG GAGCAGCAGA CAACGCCATC ATCGTCTCCG GATGTAAAAG 
     3301 TAAAACTTGA AGGGAGTGTC TTTCTTTTGG ACCGTGAGTC AAACTCAGAT GGCAGCTTTA 
     3361 ATCGTAACGA ATGGCAACAA GTTACTAAGG ATTCTGACTT CATATCTGCC AGCTGTGAGC 
     3421 AACAGCAAGA CATTAGTGTG ATGACAATTC CTGAGCATCC TGACATCCAT GATTTAGAGA 
     3481 AGTCTGTTTG GGAATTAGAA GGGATGCCAC AGGACACATA CAGCCAGCAG CTCCATAGCC 
     3541 AGATACCAGA ATCTTCTCTG AATGAAATAC AAGCACAGTC TTCAGATCAG TTACCGTTGC 
     3601 AGTCGGAACT GAAAGAGTTT GAGTCTTCTG TTTCCCAGAC AAATGAAAGC TACTTTCCTT 
     3661 TTGATGATGA ACTTACACAA GATAGCATTG TGGAAGAGCT GGTGCTTATG GAGCAGCAGA 
     3721 TGTCAATGAA TAACTCCCAT TCTTATGGTA ACTGCTTGGG AATGACCCTT CAGAGTCAGT 
     3781 CAGTAACTCC AGGAGCTCCA ATGTCATCTC ATGGTTCCAG CTCTCACTTC TATCATCCAA 
     3841 TCCATAGCAA TGGCACTCCA ATTCACACAC CTACACCCAC ACCCACTCCT ACACCAACTC 
     3901 CGACCCCAAC TCCAACCCCA ACATCTGAAA TGATTGCTGG GTCTCAGAGT CTGTCACGGG 
     3961 AGAGCCCTTG TTCCAGGCTT GCCCAGACAA CACCTGTGGA TAGTGCTTTA GGAAGTAGCC 
     4021 GACACACACC CATTGGTACT CCTCATTCTA ATTGCAGCAG TAGCGTCCCT CCAAGCCCTG 
     4081 TTGAATGCAG GAATCCATTT GCATTCACCC CAATAAGCTC CAGTATGGCA TATCACGATG 
     4141 CCAGCATTGT CTCAAGCAGT CCTGTAAAAC CAATGCAAAG ACCCATGGCC ACTCACCCTG 
     4201 ATAAAACCAA GCTTGAATGG ATGAATAATG GGTATGGTGG GGTTGGTAAC TCATCAGTTT 
     4261 CTGGTCACGG CATTCTCCCA AGCTATCAGG AACTAGTAGA AGATCGTTTC AGGAAACCTC 
     4321 ATGCTTTTGC TGTGCCCGGA CAGTCGTATC AGTCTCAGTC CAGACACCAT GACACTCATT 
     4381 TTGGTCGTTT GACTCCCGTC TCTCCTGTGC AGCATCAAGG TGCCACTGTA AACACCAACA 
     4441 AGCAAGAAGG GTTTGCAGTC CCCGCTCCTC TTGATAATAA AGGAACTAAT TCATCTGCCA 
     4501 GCAGCAACTT CAGGTGCCGG AGTGTAAGCC CTGCTGTCCA TCGCCAACGG AATCTTAGTG 
     4561 GAAGCACCCT CTATCCTGTG TCTAATATCC CACGGTCTAA TGTAACCCCC TTTGGAAGTC 
     4621 CAGTGACCCC AGAAGTTCAT GTTTTCACAA ATGTTCACAC AGATGCATGT GCCAACAACA 
     4681 TAGCTCAAAG AAGTCAGTCA GTTCCATTAA CAGTCATGAT GCAGACAGCC TTCCCTAATG 
     4741 CTCTGCAGAA GCAAACAAAC AGTAAAAAAA TCACTAATGT TTTGTTGAGT AAACTCGATT 
     4801 CTGACAATGA TGATGCAGTG AGAGGTTTGG GAATGAACAA CGTGCCCTCC AATTACACAG 
     4861 CCCGGATGAA TCTCACTCAG ATTTTGGAAA CTTCCCCTGT TTTTCCTAGT GCCAATTCAC 
     4921 AAAATATGAT CGATTCCAGC ACTTCTGTTT ATGAATTCCA AACACCATCT TACCTCACCA 
     4981 AAAGTAATAG CACCGATCAG ATCAGTTTTT CTCCTGGAGA TAATCAAGCA CAATCTGAAA 
     5041 TTGGAGAACA GCAGTTAGAT TTCAATAGCA CTGTTAAAGA CCTGTTGAGT GGAGACAACC 
     5101 TGCAAACCAG CCAGCAGCTG GGAGGTCAGG TAGCATCTGA TCTCACCAAC ACTGCATCTG 
     5161 ATTTCCCTAG CGACATCAGG TTGTCTTCTG ACCTCTCAGG CAGCATCAAT GATTTGAACA 
     5221 CCTTAGACCC AAATCTACTG TTTGATCCAG GTCGTCAGCA GGGACAGGAT GATGAAGCCA 
     5281 CACTGGAAGA ATTAAAGAAT GACCCACTGT TTCAACAGAT TTGCAGTGAG TCAATGAACT 
     5341 CTATGACTTC ATCAGGTTTT GAATGGATAG AAAGCAAGGA CCATCCTACT GTTGAAATGT 
     5401 TGGGAACGCG TACGCGGCCG CTCGAGCAGA AACTCATCTC AGAAGAGGAT CTGGCAGCAA 
     5461 ATGATATCCT GGATTACAAG GATGACGACG ATAAGGTTTA AACGGCCGGC CGCGGTCATA 
     5521 GCTGTTTCCT GAACAGATCC CGGGTGGCAT CCCTGTGACC CCTCCCCAGT GCCTCTCCTG 
     5581 GCCCTGGAAG TTGCCACTCC AGTGCCCACC AGCCTTGTCC TAATAAAATT AAGTTGCATC 
     5641 ATTTTGTCTG ACTAGGTGTC CTTCTATAAT ATTATGGGGT GGAGGGGGGT GGTATGGAGC 
     5701 AAGGGGCAAG TTGGGAAGAC AACCTGTAGG GCCTGCGGGG TCTATTGGGA ACCAAGCTGG 
     5761 AGTGCAGTGG CACAATCTTG GCTCACTGCA ATCTCCGCCT CCTGGGTTCA AGCGATTCTC 
     5821 CTGCCTCAGC CTCCCGAGTT GTTGGGATTC CAGGCATGCA TGACCAGGCT CAGCTAATTT 
     5881 TTGTTTTTTT GGTAGAGACG GGGTTTCACC ATATTGGCCA GGCTGGTCTC CAACTCCTAA 
     5941 TCTCAGGTGA TCTACCCACC TTGGCCTCCC AAATTGCTGG GATTACAGGC GTGAACCACT 
     6001 GCTCCCTTCC CTGTCCTTCT GATTTTAAAA TAACTATACC AGCAGGAGGA CGTCCAGACA 
     6061 CAGCATAGGC TACCTGGCCA TGCCCAACCG GTGGGACATT TGAGTTGCTT GCTTGGCACT 
     6121 GTCCTCTCAT GCGTTGGGTC CACTCAGTAG ATGCCTGTTG AATTGGGTAC GCGGCCAGCG 
     6181 GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA 
     6241 CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC 
     6301 GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC 
     6361 AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG 
     6421 CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT 
     6481 CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA 
     6541 GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC 
     6601 CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC 
     6661 AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT 
     6721 GAAGTGGTGG CCTAACTACG GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT 
     6781 GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC 
     6841 TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA 
     6901 AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA 
     6961 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA 
     7021 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAACCT GAGGCTATGG CAGGGCCTGC 
     7081 CGCCCCGACG TTGGCTGCGA GCCCTGGGCC TTCACCCGAA CTTGGGGGGT GGGGTGGGGA 
     7141 AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA ATGGGGTCTC GGTGGGGTAT CGACAGAGTG 
     7201 CCAGCCCTGG GACCGAACCC CGCGTTTATG AACAAACGAC CCAACACCGT GCGTTTTATT 
     7261 CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC 
     7321 AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA CTCCAGCATG AGATCCCCGC GCTGGAGGAT 
     7381 CATCCAGCCG GCGTCCCGGA AAACGATTCC GAAGCCCAAC CTTTCATAGA AGGCGGCGGT 
     7441 GGAATCGAAA TCTCGTGATG GCAGGTTGGG CGTCGCTTGG TCGGTCATTT CGAACCCCAG 
     7501 AGTCCCGCTC AGAAGAACTC GTCAAGAAGG CGATAGAAGG CGATGCGCTG CGAATCGGGA 
     7561 GCGGCGATAC CGTAAAGCAC GAGGAAGCGG TCAGCCCATT CGCCGCCAAG CTCTTCAGCA 
     7621 ATATCACGGG TAGCCAACGC TATGTCCTGA TAGCGATCCG CCACACCCAG CCGGCCACAG 
     7681 TCGATGAATC CAGAAAAGCG GCCATTTTCC ACCATGATAT TCGGCAAGCA GGCATCGCCA 
     7741 TGGGTCACGA CGAGATCCTC GCCGTCGGGC ATGCTCGCCT TGAGCCTGGC GAACAGTTCG 
     7801 GCTGGCGCGA GCCCCTGATG CTCTTCGTCC AGATCATCCT GATCGACAAG ACCGGCTTCC 
     7861 ATCCGAGTAC GTGCTCGCTC GATGCGATGT TTCGCTTGGT GGTCGAATGG GCAGGTAGCC 
     7921 GGATCAAGCG TATGCAGCCG CCGCATTGCA TCAGCCATGA TGGATACTTT CTCGGCAGGA 
     7981 GCAAGGTGAG ATGACAGGAG ATCCTGCCCC GGCACTTCGC CCAATAGCAG CCAGTCCCTT 
     8041 CCCGCTTCAG TGACAACGTC GAGCACAGCT GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC 
     8101 GATAGCCGCG CTGCCTCGTC TTGCAGTTCA TTCAGGGCAC CGGACAGGTC GGTCTTGACA 
     8161 AAAAGAACCG GGCGCCCCTG CGCTGACAGC CGGAACACGG CGGCATCAGA GCAGCCGATT 
     8221 GTCTGTTGTG CCCAGTCATA GCCGAATAGC CTCTCCACCC AAGCGGCCGG AGAACCTGCG 
     8281 TGCAATCCAT CTTGTTCAAT CATGCGAAAC GATCCTCATC CTGTCTCTTG ATCGATCTTT 
     8341 GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC TCACTACTTC TGGAATAGCT CAGAGGCCGA 
     8401 GGCGGCCTCG GCCTCTGCAT AAATAAAAAA AATTAGTCAG CCATGGGGCG GAGAATGGGC 
     8461 GGAACTGGGC GGAGTTAGGG GCGGGATGGG CGGAGTTAGG GGCGGGACTA TGGTTGCTGA 
     8521 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     8581 CCTGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     8641 GGACTTTCCA CACCCTAACT GACACACATT CCACAGCTGG TTCTTTCCGC CTCAGGACTC 
     8701 TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA 
     8761 TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG 
     8821 CCACCTGACG CGCCCTGTAG CGGCGCATTA AGCGCGGCGG GTGTGGTGGT TACGCGCAGC 
     8881 GTGACCGCTA CACTTGCCAG CGCCCTAGCG CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT 
     8941 CTCGCCACGT TCGCCGGCTT TCCCCGTCAA GCTCTAAATC GGGGGCTCCC TTTAGGGTTC 
     9001 CGATTTAGTG CTTTACGGCA CCTCGACCCC AAAAAACTTG ATTAGGGTGA TGGTTCACGT 
     9061 AGTGGGCCAT CGCCCTGATA GACGGTTTTT CGCCCTTTGA CGTTGGAGTC CACGTTCTTT 
     9121 AATAGTGGAC TCTTGTTCCA AACTGGAACA ACACTCAACC CTATCTCGGT CTATTCTTTT 
     9181 GATTTATAAG GGATTTTGCC GATTTCGGCC TATTGGTTAA AAAATGAGCT GATTTAACAA 
     9241 AAATTTAACG CGAATTTT
//