LOCUS       RR214925                8001 bp    DNA     circular  SYN 
DEFINITION  Man2c1 (Myc-DDK-tagged ORF) - Rat mannosidase, alpha, class 2C, member 1 (Man2c1), (10 ug)
ACCESSION   NM_139256
VERSION     NM_139256.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4158..4249 
                     /label="Myc-DDK"  
     misc_feature    4279..4864 
                     /label="PolyA signal"  
     misc_feature    4336..4355 
                     /label="Restriction Enzyme"  
     misc_feature    5140..5812 
                     /label="ColE1"  
     misc_feature    6242..7036 
                     /label="Kan/Neo"  
     misc_feature    7071..7373 
                     /label="SV40 ori"  
     misc_feature    7459..7936 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4140..4146 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4140 
                     /label="Man2c1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGGCAGCG CCGTTCTTGA AGCACTGGCG CACCACTTTT GAGCGGGTGG 
     1081 AGAAGTTCGT GTCCCCGATC TACTTCACCG ACTGTAACCT TCGCGGCAGG CTCTTCGGGG 
     1141 ACAGCTGCCC GGTGACACTC TCCAGCTTCC TGACGCCGGA AAGGCTTCCC TATGAGAAGG 
     1201 CAGTGCAGCA GAACTTCAGC CCTGCGCAGG TCGGCGACAG CTTCGGACCC ACATGGTGGA 
     1261 CCTGCTGGTT TCGGGTAGAA CTGGTCATCC CTGAAGTTTG GGTGGGCAAG GAAGTTCATC 
     1321 TTTGCTGGGA AAGTGATGGA GAAAGCCTTG TCTGGCGTGA TGGGGAACCT GTCCAGGGTT 
     1381 TGACCAAGGA GGGTGAAAAG ACTAGTTATG TCCTGAGTGA GAGGCTGCAC GCAGCAGATC 
     1441 CTCGGAGCTT GACTCTCTAT GTGGAAGTAG CCTGCAATGG GCTTCTGGGA GCCGGGAAGG 
     1501 GAAGCATGAT CGCAGCCCCT GACCCTGAGA AGATGTTCCA GCTGAGCCAG GCTAAGCTGG 
     1561 CTGTGTTCCA CCGGGATGTC CACAATCTCC TGGTGGACCT GGAGCTGTTG CTGGGTGTGG 
     1621 CCAAGGGCCT CGGGGAGGAC AACCAGCGCA GCTTCCAGGC CCTGTACACA GCCAACCAGA 
     1681 TGGTGAACAT ATGTGACCCT GCGCAGCCTG AGACTTACCC AGCAGCCGAG GCCCTGGCCT 
     1741 CCAAGTTCTT TGGCCAACGT GGAGGTGAAA GCCAGCATAC CATCCATGCA ACAGGACACT 
     1801 GTCACATTGA TACAGCCTGG CTTTGGCCCT TCAAGGAGAC TGTGAGGAAA TGTGCCCGGA 
     1861 GCTGGTCAAC AGCTGTGAAG CTCATGGAGC GGAACACTGA GTTCACCTTT GCCTGCTCTC 
     1921 AGGCACAACA GCTCGAGTGG GTAAAGAACC AATACCCTGG TCTGTATGCC CAGCTCCAGG 
     1981 AGTTTGCCTG CCGAGGACAG TTTGTGCCTG TGGGGGGCAC CTGGGTAGAA ATGGATGGGA 
     2041 ACCTTCCCAG TGGAGAGGCC ATGGTGAGGC AGTTCCTACA GGGCCAGAAC TTCTTTCTGC 
     2101 AAGAGTTTGG AAAGATGTGC TCTGAGTTCT GGCTGCCAGA TACATTTGGC TACTCAGCAC 
     2161 AGCTCCCCCA GATCATGCAA GGCTGTGGCA TCAAACGCTT CCTGACACAG AAACTGAGCT 
     2221 GGAACTTGGT CAACTCCTTC CCACACCATA CCTTCTTCTG GGAGGGGCTG GATGGCTCCC 
     2281 AAGTGCTGGT ACACTTCCCC CCTGGAGACT CATATGGAAT GCAGGGCAGT GTGGAAGAGG 
     2341 TGCTGAAGAC TGTGACCAAC AACCGGGACA AGGGACGGAC CAACCACAGT GGCTTCCTCT 
     2401 TTGGCTTCGG GGATGGGGGT GGTGGCCCCA CACAGACCAT GTTGGACCGA TTGAAGCGGC 
     2461 TGGGCAACAC TGATGGGCAG CCCAGGGTGC AGCTGTCTTC TCCTGGACAG CTCTTCACAG 
     2521 CACTGGAGAG AGACTCAGGG CAGCTGTGCA CATGGGTTGG GGAACTCTTC TTGGAGCTGC 
     2581 ACAATGGCAC CTATACTACC CATGCCCAGC TCAAGAAGGG GAACCGAGAA TGTGAGCAGA 
     2641 TTCTTCATGA CGTGGAGCTG CTCAGCAGCC TTGCCCTAGC CCGAAGTGCC CAGTTCCTGT 
     2701 ACCCGGCAGT CCAGCTGCAG CGCCTCTGGA GGCTCCTACT CCTCAACCAG TTCCATGATG 
     2761 TGGTGACTGG AAGCTGCATT CAGCTGGTGG CAGAAGATGC CATGAACTAC TATGAAGACA 
     2821 TCCGTTCTCA TGGCAATACG CTGCTCAGTG CTGCAGCCGC AGCCTTGTGT GCTGGGGAGC 
     2881 CAGGTCCCAA GGGCCTCCGT CATTATCAAC ACACTGCCCT GGAAGCGCAC AGAAGTGTTG 
     2941 GCACTACCCA AGGCCTTGTG GGGCTCACAC GCCTAGCCCT GGTGACAGTG CCCAGTATTG 
     3001 GCTATGCTCC TGCTCCCACC CCTACTTCAC TGCAGCCCCT CCTACCCCAG CAGCCCGTGT 
     3061 TTGTGATGCA GGAGACTGAC GGTTCCGTGA CGCTGGACAA TGGCATCATC CGGGTGAGAC 
     3121 TGGATCCAAC TGGCTGTCTT ACTTCCCTGG TCCTGGTGGC TTCTGGCAGG GAAGCCATTG 
     3181 CTGAGGGTGC CCTGGGGAAT CAGTTTGTGC TGTTTGATGA TGTGCCACTG TACTGGGATG 
     3241 CATGGGACGT CATGGACTAC CATCTAGAGA CACGGAAGCC TGTGCGGGGC CAGGCAGGTA 
     3301 CCCTGGCAGT AGGCGCTGAA GGTGGCCTTC GGGGTAGTGC CTGGTTCCTG CTACAGATCA 
     3361 GCCCCAACAG TCGGCTCAGC CAGGAAGTCG TGCTGGATGT TGGTTGCCCC TATGTCCGCT 
     3421 TCCACACGGA GGTGCACTGG CACGAGACCC ACAAGTTCCT GAAGGTGGAG TTCCCAGCTC 
     3481 GAGTACGGAG CCCCCAGGCT ACCTATGAGA TCCAGTTTGG GCACCTTCAG GAGGCCGACC 
     3541 CACAACAACA CTCCTGGGAC TGGGCTCGCT ATGAGGTGTG GGCTCATCGC TGGATAGATC 
     3601 TGTCCGAGTG TGACTTTGGG CTGGCCCTGC TCAACAACTG CAAGTATGGA ACGTCAGTAC 
     3661 GAGGCAACGT CCTCAGCCTC TCACTGCTGC GTGCACCCAA GGCTCCAGAT GTTACTGCTG 
     3721 ACATGGGGCG CCATGAATTC ACTTATGCCT TGATGCCACA CAAGGGTTCT TTCCAAGAAG 
     3781 CTGGTGTTAT CCAGGCTGCC TACAACCTCA ACTTTCCCCT CTTGGCACTG CCAGCTCCAG 
     3841 GCCCAGCCCC TGATACCACC TGGAGCGCCT TTTCCGTGTC TTCACCAGCA GTCGTGTTGG 
     3901 AGACCATCAA GCAGGCCGAG AAGAGCCATC AGCACCGCAC ACTGGTTCTA AGGCTGTATG 
     3961 AGGCTCATGG CAGCCATGTG GACTGCTGGC TACACACATC ATTGCCTGTT CAGGAGGCCA 
     4021 CCCTTTGTGA CCTCCTGGAG CAGCGAGACC CTACCGGTCA CCTGTCCCTT CAGGACAACC 
     4081 GCCTGAAGCT CACCTTTTCT CCCTTTCAAG TGAGGTCCTT ATTGCTTGTG CTTCAGCCTC 
     4141 CAGCAAACAC GCGTACGCGG CCGCTCGAGC AGAAACTCAT CTCAGAAGAG GATCTGGCAG 
     4201 CAAATGATAT CCTGGATTAC AAGGATGACG ACGATAAGGT TTAAACGGCC GGCCGCGGTC 
     4261 ATAGCTGTTT CCTGAACAGA TCCCGGGTGG CATCCCTGTG ACCCCTCCCC AGTGCCTCTC 
     4321 CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC ACCAGCCTTG TCCTAATAAA ATTAAGTTGC 
     4381 ATCATTTTGT CTGACTAGGT GTCCTTCTAT AATATTATGG GGTGGAGGGG GGTGGTATGG 
     4441 AGCAAGGGGC AAGTTGGGAA GACAACCTGT AGGGCCTGCG GGGTCTATTG GGAACCAAGC 
     4501 TGGAGTGCAG TGGCACAATC TTGGCTCACT GCAATCTCCG CCTCCTGGGT TCAAGCGATT 
     4561 CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA TTCCAGGCAT GCATGACCAG GCTCAGCTAA 
     4621 TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC ACCATATTGG CCAGGCTGGT CTCCAACTCC 
     4681 TAATCTCAGG TGATCTACCC ACCTTGGCCT CCCAAATTGC TGGGATTACA GGCGTGAACC 
     4741 ACTGCTCCCT TCCCTGTCCT TCTGATTTTA AAATAACTAT ACCAGCAGGA GGACGTCCAG 
     4801 ACACAGCATA GGCTACCTGG CCATGCCCAA CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC 
     4861 ACTGTCCTCT CATGCGTTGG GTCCACTCAG TAGATGCCTG TTGAATTGGG TACGCGGCCA 
     4921 GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA 
     4981 TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC 
     5041 CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG 
     5101 CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG 
     5161 AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT 
     5221 TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT 
     5281 GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG 
     5341 CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT 
     5401 GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT 
     5461 CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT 
     5521 GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC 
     5581 CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC 
     5641 TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG 
     5701 TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA 
     5761 AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA CCTGAGGCTA TGGCAGGGCC 
     5821 TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG GCCTTCACCC GAACTTGGGG GGTGGGGTGG 
     5881 GGAAAAGGAA GAAACGCGGG CGTATTGGCC CCAATGGGGT CTCGGTGGGG TATCGACAGA 
     5941 GTGCCAGCCC TGGGACCGAA CCCCGCGTTT ATGAACAAAC GACCCAACAC CGTGCGTTTT 
     6001 ATTCTGTCTT TTTATTGCCG TCATAGCGCG GGTTCCTTCC GGTATTGTCT CCTTCCGTGT 
     6061 TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA GAACTCCAGC ATGAGATCCC CGCGCTGGAG 
     6121 GATCATCCAG CCGGCGTCCC GGAAAACGAT TCCGAAGCCC AACCTTTCAT AGAAGGCGGC 
     6181 GGTGGAATCG AAATCTCGTG ATGGCAGGTT GGGCGTCGCT TGGTCGGTCA TTTCGAACCC 
     6241 CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA AGGCGATAGA AGGCGATGCG CTGCGAATCG 
     6301 GGAGCGGCGA TACCGTAAAG CACGAGGAAG CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA 
     6361 GCAATATCAC GGGTAGCCAA CGCTATGTCC TGATAGCGAT CCGCCACACC CAGCCGGCCA 
     6421 CAGTCGATGA ATCCAGAAAA GCGGCCATTT TCCACCATGA TATTCGGCAA GCAGGCATCG 
     6481 CCATGGGTCA CGACGAGATC CTCGCCGTCG GGCATGCTCG CCTTGAGCCT GGCGAACAGT 
     6541 TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG TCCAGATCAT CCTGATCGAC AAGACCGGCT 
     6601 TCCATCCGAG TACGTGCTCG CTCGATGCGA TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA 
     6661 GCCGGATCAA GCGTATGCAG CCGCCGCATT GCATCAGCCA TGATGGATAC TTTCTCGGCA 
     6721 GGAGCAAGGT GAGATGACAG GAGATCCTGC CCCGGCACTT CGCCCAATAG CAGCCAGTCC 
     6781 CTTCCCGCTT CAGTGACAAC GTCGAGCACA GCTGCGCAAG GAACGCCCGT CGTGGCCAGC 
     6841 CACGATAGCC GCGCTGCCTC GTCTTGCAGT TCATTCAGGG CACCGGACAG GTCGGTCTTG 
     6901 ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC AGCCGGAACA CGGCGGCATC AGAGCAGCCG 
     6961 ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT AGCCTCTCCA CCCAAGCGGC CGGAGAACCT 
     7021 GCGTGCAATC CATCTTGTTC AATCATGCGA AACGATCCTC ATCCTGTCTC TTGATCGATC 
     7081 TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC TCCTCACTAC TTCTGGAATA GCTCAGAGGC 
     7141 CGAGGCGGCC TCGGCCTCTG CATAAATAAA AAAAATTAGT CAGCCATGGG GCGGAGAATG 
     7201 GGCGGAACTG GGCGGAGTTA GGGGCGGGAT GGGCGGAGTT AGGGGCGGGA CTATGGTTGC 
     7261 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     7321 ACACCTGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     7381 TGGGGACTTT CCACACCCTA ACTGACACAC ATTCCACAGC TGGTTCTTTC CGCCTCAGGA 
     7441 CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC 
     7501 ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA 
     7561 GTGCCACCTG ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG CGGGTGTGGT GGTTACGCGC 
     7621 AGCGTGACCG CTACACTTGC CAGCGCCCTA GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC 
     7681 TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG 
     7741 TTCCGATTTA GTGCTTTACG GCACCTCGAC CCCAAAAAAC TTGATTAGGG TGATGGTTCA 
     7801 CGTAGTGGGC CATCGCCCTG ATAGACGGTT TTTCGCCCTT TGACGTTGGA GTCCACGTTC 
     7861 TTTAATAGTG GACTCTTGTT CCAAACTGGA ACAACACTCA ACCCTATCTC GGTCTATTCT 
     7921 TTTGATTTAT AAGGGATTTT GCCGATTTCG GCCTATTGGT TAAAAAATGA GCTGATTTAA 
     7981 CAAAAATTTA ACGCGAATTT T
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