LOCUS       RR214908                8997 bp    DNA     circular  SYN 
DEFINITION  Col1a2 (Myc-DDK-tagged ORF) - Rat collagen, type I, alpha 2 (Col1a2), (10 ug)
ACCESSION   NM_053356
VERSION     NM_053356.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5154..5245 
                     /label="Myc-DDK"  
     misc_feature    5275..5860 
                     /label="PolyA signal"  
     misc_feature    5332..5351 
                     /label="Restriction Enzyme"  
     misc_feature    6136..6808 
                     /label="ColE1"  
     misc_feature    7238..8032 
                     /label="Kan/Neo"  
     misc_feature    8067..8369 
                     /label="SV40 ori"  
     misc_feature    8455..8932 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5136..5142 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5136 
                     /label="Col1a2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCTCAGCTTT GTGGATACGC GGACTCTGTT GCTGCTTGCA GTAACGTCGT 
     1081 GCCTAGCAAC ATGCCAATCT TTACAAATGG GATCTGTACG GAAGGGCCCC ACTGGAGACA 
     1141 GAGGACCGCG TGGACAAAGG GGCCCAGCAG GTCCCCGAGG CAGAGATGGT GTTGATGGTC 
     1201 CCGTTGGCCC TCCTGGTCCC CCTGGTGCCC CTGGCCCCCC TGGTCCCCCT GGCCCCCCTG 
     1261 GTCTTACTGG GAACTTTGCT GCTCAGTATT CTGACAAAGG AGTTTCAGCT GGCCCCGGAC 
     1321 CAATGGGCTT AATGGGACCC AGAGGCCCTC CTGGTGCTGT CGGAGCCCCT GGCCCTCAAG 
     1381 GTTTCCAAGG ACCTGCTGGT GAGCCTGGTG AGCCCGGACA AACGGGTCCT GCAGGTTCCC 
     1441 GTGGGCCAGC TGGCCCTCCT GGCAAGGCCG GTGAAGATGG TCACCCTGGA AAACCTGGAA 
     1501 GACCTGGCGA GAGAGGAGTC GTTGGGCCAC AAGGTGCTCG TGGTTTCCCT GGGACTCCTG 
     1561 GACTTCCTGG CTTCAAAGGC ATTCGAGGAC ACAACGGTCT GGATGGATTG AAAGGACAGC 
     1621 CTGGTGCCCA GGGTGTGAAG GGTGAACCCG GTGCCCCTGG AGAGAATGGA ACCCCAGGTC 
     1681 AAGCAGGAGC CCGAGGGCTT CCTGGTGAGA GAGGGCGTGT CGGAGCTCCT GGTCCAGCTG 
     1741 GTGCCCGTGG CAGTGATGGC AGTGTTGGCC CCGTCGGCCC TGCTGGTCCC ATTGGGTCTG 
     1801 CTGGTCCTCC TGGTTTCCCA GGTGCTCCTG GTCCCAAGGG TGAACTTGGG CCTGTTGGTA 
     1861 ACCCTGGCCC TGCTGGTCCT GCTGGTCCCC GTGGTGAAGC GGGTCTTCCG GGTCTTTCTG 
     1921 GCCCCGTTGG ACCTCCTGGC AACCCTGGAG CAAATGGCCT CACTGGTGCC AAGGGAGCCA 
     1981 CTGGACTCCC TGGTGTTGCT GGGGCTCCTG GTCTCCCCGG TCCCCGTGGT ATTCCTGGCC 
     2041 CCGTTGGTGC TGCTGGTGCC ACTGGTCCCA GAGGACTTGT TGGTGAACCT GGTCCTGCTG 
     2101 GCTCCAAAGG AGAGACTGGT AACAAGGGTG AGCCTGGCTC TGCTGGAGCC CAAGGTCCTC 
     2161 CTGGTCCCAG CGGTGAAGAA GGAAAGAGAG GGTCCCCTGG AGAGCCTGGA TCTGCTGGTC 
     2221 CTGCAGGGCC TCCAGGGCTG AGAGGCAGCC CTGGTTCTCG AGGTCTTCCT GGAGCTGATG 
     2281 GCAGAGCTGG TGTGATGGGC CCTCCTGGCA ATCGTGGTTC AACTGGCCCT GCTGGAGTCC 
     2341 GAGGTCCTAA TGGAGATGCC GGTCGCCCTG GAGAACCTGG TCTCATGGGA CCCAGAGGTC 
     2401 TTCCCGGGTC TCCTGGAAAT GTTGGCCCAG CTGGTAAAGA AGGCCCTGTG GGTCTCCCTG 
     2461 GCATCGATGG CAGACCTGGC CCAATTGGCC CAGCTGGACC AAGAGGTGAA GCTGGCAACA 
     2521 TCGGATTCCC TGGACCCAAA GGCCCCTCTG GTGATCCTGG CAAACCTGGT GAGAAAGGCC 
     2581 ATCCCGGTCT CGCTGGTGCT CGGGGAGCTC CAGGTCCCGA CGGCAACAAT GGTGCTCAGG 
     2641 GCCCTCCTGG ACCTCAGGGT GTTCAAGGTG GCAAAGGTGA ACAGGGCCCT GCTGGTCCTC 
     2701 CAGGCTTCCA GGGTCTCCCG GGTCCCTCTG GTACCGCTGG AGAAGTTGGC AAGCCGGGAG 
     2761 AAAGGGGTCT TCCCGGTGAA TTCGGTCTCC CTGGTCCTGC TGGTCCAAGA GGAGAACGTG 
     2821 GTCCCCCAGG TGAGAGTGGA GCTGCTGGTC CTTCTGGTCC TATTGGAATC CGAGGTCCCT 
     2881 CTGGAGCCCC AGGGCCTGAT GGGAACAAGG GTGAAGCTGG TGCAGTCGGT GCTCCAGGCT 
     2941 CTGCTGGTGC CTCTGGTCCT GGTGGGCTTC CAGGAGAGAG GGGTGCTGCT GGCATACCTG 
     3001 GAGGCAAAGG AGAAAAGGGT GAAACTGGTC TCAGAGGTGA AATCGGCAAC CCTGGTAGAG 
     3061 ATGGTGCTCG AGGTGCTCCT GGTGCTATAG GTGCCCCTGG TCCCGCTGGA GCCTCAGGTG 
     3121 ACCGGGGCGA AGCTGGTGCT GCTGGTCCTT CTGGCCCTGC TGGTCCTCGA GGTAGCCCTG 
     3181 GTGAACGTGG TGAGGTTGGT CCTGCTGGCC CCAATGGATT TGCTGGTCCT GCTGGTTCTG 
     3241 CTGGCCAACC TGGTGCTAAA GGAGAAAAGG GAACCAAAGG GCCTAAGGGA GAAAATGGCA 
     3301 TCGTTGGTCC AACCGGACCT GTCGGAGCTG CTGGCCCATC TGGTCCTAAT GGCCCACCTG 
     3361 GCCCTGCTGG AAGTCGTGGT GATGGAGGGC CCCCTGGTAT GACTGGCTTC CCTGGTGCTG 
     3421 CTGGACGGAC TGGTCCCCCC GGACCCAGTG GTATTACTGG CCCCCCTGGT CCCCCTGGTG 
     3481 CTGCTGGTAA AGAAGGTATT CGTGGTCCCC GTGGTGACCA AGGTCCAGTT GGCCGAACTG 
     3541 GAGAAATAGG TGCATCTGGT CCCCCTGGAT TTGCTGGCGA GAAGGGTCCT TCTGGAGAAC 
     3601 CTGGTACCAC AGGACCCCCT GGCACTGCAG GTCCTCAGGG TCTTCTTGGT GCTCCCGGTA 
     3661 TTCTGGGTCT CCCTGGCTCT CGAGGTGAAC GTGGCCAGCC AGGTATTGCT GGTGCTCTGG 
     3721 GTGAACCTGG TCCTCTGGGC ATTGCAGGCC CTCCTGGGGC CCGTGGTCCC CCTGGTGCTG 
     3781 TGGGCAGTCC TGGAGTTAAT GGTGCCCCTG GTGAAGCCGG TCGTGATGGT AACCCTGGCA 
     3841 GTGATGGTCC CCCAGGTCGT GATGGTCAAC CCGGACACAA GGGAGAGCGT GGTTACCCTG 
     3901 GCAACATTGG TCCCACTGGT GCTGCAGGTG CTCCTGGTCC TCATGGTTCT GTGGGTCCTG 
     3961 CTGGGAAACA TGGAAACCGT GGTGAGCCTG GTCCTGCTGG TTCTGTTGGT CCCGTTGGTG 
     4021 CTGTCGGTCC AAGAGGTCCT AGTGGCCCTC AAGGTATTCG AGGTGACAAG GGAGAGCCTG 
     4081 GTGACAAAGG GGCCAGAGGT CTTCCTGGCT TAAAAGGACA CAATGGATTA CAGGGTCTTC 
     4141 CTGGTCTTGC TGGGCTGCAT GGTGACCAAG GAGCTCCTGG TCCCGTGGGT CCTGCTGGTC 
     4201 CCAGGGGTCC TGCTGGTCCT TCTGGCCCCA TTGGCAAAGA TGGTAGATCT GGACATCCTG 
     4261 GACCAGTCGG TCCTGCTGGC GTTCGTGGCT CTCAGGGTAG CCAAGGTCCC GCTGGTCCCC 
     4321 CTGGTCCCCC TGGGCCTCCT GGGCCTCCTG GTGTCAGCGG AGGAGGCTAT GACTTTGGTT 
     4381 TCGAAGGAGG CTTCTACAGG GCTGACCAGC CTCGCTCACA GCCTTCACTC AGACCCAAGG 
     4441 ACTATGAAGT TGATGCAACT CTGAAATCTC TCAATAACCA AATCGAGACC CTTCTCACTC 
     4501 CTGAAGGCTC TAGAAAGAAC CCTGCCCGCA CATGCCGTGA CTTAAGACTC AGCCACCCAG 
     4561 AGTGGAAGAG CGATTACTAC TGGATTGACC CTAACCAAGG ATGCACTATG GATGCCATCA 
     4621 AAGTGTACTG CGATTTCTCT ACTGGTGAAA CCTGCATCCA GGCCCAACCT GTCAACACCC 
     4681 CAGCCAAGAA TGCATACAGC CGTGCCCAGG CCAACAAGCA TGTCTGGTTA GGAGAGACCA 
     4741 TCAATGGTGG CAGCCAGTTT GAATACAACG CAGAAGGGGT GTCCTCCAAG GAAATGGCAA 
     4801 CTCAGCTCGC CTTCATGCGC CTGCTAGCCA ACCGTGCTTC TCAGAACATC ACCTACCACT 
     4861 GCAAGAACAG CATTGCGTAC CTGGACGAGG AGACAGGCCG CCTGAATAAG GCTGTCATTC 
     4921 TGCAGGGCTC CAACGACGTC GAACTTGTTG CTGAGGGCAA CAGCAGATTC ACCTACACTG 
     4981 TCCTTGTCGA TGGCTGCTCC AAAAAGACAA ATGAATGGGA CAAGACAGTC ATTGAATACA 
     5041 AAACGAATAA GCCATCTCGC CTGCCATTCC TTGACATTGC ACCTCTGGAC ATCGGTGGTA 
     5101 CTAACCAAGA ATTCCGTGTG GAGGTTGGCC CTGTCTGTTT CAAAACGCGT ACGCGGCCGC 
     5161 TCGAGCAGAA ACTCATCTCA GAAGAGGATC TGGCAGCAAA TGATATCCTG GATTACAAGG 
     5221 ATGACGACGA TAAGGTTTAA ACGGCCGGCC GCGGTCATAG CTGTTTCCTG AACAGATCCC 
     5281 GGGTGGCATC CCTGTGACCC CTCCCCAGTG CCTCTCCTGG CCCTGGAAGT TGCCACTCCA 
     5341 GTGCCCACCA GCCTTGTCCT AATAAAATTA AGTTGCATCA TTTTGTCTGA CTAGGTGTCC 
     5401 TTCTATAATA TTATGGGGTG GAGGGGGGTG GTATGGAGCA AGGGGCAAGT TGGGAAGACA 
     5461 ACCTGTAGGG CCTGCGGGGT CTATTGGGAA CCAAGCTGGA GTGCAGTGGC ACAATCTTGG 
     5521 CTCACTGCAA TCTCCGCCTC CTGGGTTCAA GCGATTCTCC TGCCTCAGCC TCCCGAGTTG 
     5581 TTGGGATTCC AGGCATGCAT GACCAGGCTC AGCTAATTTT TGTTTTTTTG GTAGAGACGG 
     5641 GGTTTCACCA TATTGGCCAG GCTGGTCTCC AACTCCTAAT CTCAGGTGAT CTACCCACCT 
     5701 TGGCCTCCCA AATTGCTGGG ATTACAGGCG TGAACCACTG CTCCCTTCCC TGTCCTTCTG 
     5761 ATTTTAAAAT AACTATACCA GCAGGAGGAC GTCCAGACAC AGCATAGGCT ACCTGGCCAT 
     5821 GCCCAACCGG TGGGACATTT GAGTTGCTTG CTTGGCACTG TCCTCTCATG CGTTGGGTCC 
     5881 ACTCAGTAGA TGCCTGTTGA ATTGGGTACG CGGCCAGCGG CGAGCGGTAT CAGCTCACTC 
     5941 AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC 
     6001 AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG 
     6061 GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC 
     6121 GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT 
     6181 TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT 
     6241 TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG 
     6301 CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT 
     6361 TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT 
     6421 TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG 
     6481 CTACACTAGA AGAACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA 
     6541 AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT 
     6601 TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC 
     6661 TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT 
     6721 ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA 
     6781 AAGTATATAT GAGTAACCTG AGGCTATGGC AGGGCCTGCC GCCCCGACGT TGGCTGCGAG 
     6841 CCCTGGGCCT TCACCCGAAC TTGGGGGGTG GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA 
     6901 TTGGCCCCAA TGGGGTCTCG GTGGGGTATC GACAGAGTGC CAGCCCTGGG ACCGAACCCC 
     6961 GCGTTTATGA ACAAACGACC CAACACCGTG CGTTTTATTC TGTCTTTTTA TTGCCGTCAT 
     7021 AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG 
     7081 GGCGAAGAAC TCCAGCATGA GATCCCCGCG CTGGAGGATC ATCCAGCCGG CGTCCCGGAA 
     7141 AACGATTCCG AAGCCCAACC TTTCATAGAA GGCGGCGGTG GAATCGAAAT CTCGTGATGG 
     7201 CAGGTTGGGC GTCGCTTGGT CGGTCATTTC GAACCCCAGA GTCCCGCTCA GAAGAACTCG 
     7261 TCAAGAAGGC GATAGAAGGC GATGCGCTGC GAATCGGGAG CGGCGATACC GTAAAGCACG 
     7321 AGGAAGCGGT CAGCCCATTC GCCGCCAAGC TCTTCAGCAA TATCACGGGT AGCCAACGCT 
     7381 ATGTCCTGAT AGCGATCCGC CACACCCAGC CGGCCACAGT CGATGAATCC AGAAAAGCGG 
     7441 CCATTTTCCA CCATGATATT CGGCAAGCAG GCATCGCCAT GGGTCACGAC GAGATCCTCG 
     7501 CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG AACAGTTCGG CTGGCGCGAG CCCCTGATGC 
     7561 TCTTCGTCCA GATCATCCTG ATCGACAAGA CCGGCTTCCA TCCGAGTACG TGCTCGCTCG 
     7621 ATGCGATGTT TCGCTTGGTG GTCGAATGGG CAGGTAGCCG GATCAAGCGT ATGCAGCCGC 
     7681 CGCATTGCAT CAGCCATGAT GGATACTTTC TCGGCAGGAG CAAGGTGAGA TGACAGGAGA 
     7741 TCCTGCCCCG GCACTTCGCC CAATAGCAGC CAGTCCCTTC CCGCTTCAGT GACAACGTCG 
     7801 AGCACAGCTG CGCAAGGAAC GCCCGTCGTG GCCAGCCACG ATAGCCGCGC TGCCTCGTCT 
     7861 TGCAGTTCAT TCAGGGCACC GGACAGGTCG GTCTTGACAA AAAGAACCGG GCGCCCCTGC 
     7921 GCTGACAGCC GGAACACGGC GGCATCAGAG CAGCCGATTG TCTGTTGTGC CCAGTCATAG 
     7981 CCGAATAGCC TCTCCACCCA AGCGGCCGGA GAACCTGCGT GCAATCCATC TTGTTCAATC 
     8041 ATGCGAAACG ATCCTCATCC TGTCTCTTGA TCGATCTTTG CAAAAGCCTA GGCCTCCAAA 
     8101 AAAGCCTCCT CACTACTTCT GGAATAGCTC AGAGGCCGAG GCGGCCTCGG CCTCTGCATA 
     8161 AATAAAAAAA ATTAGTCAGC CATGGGGCGG AGAATGGGCG GAACTGGGCG GAGTTAGGGG 
     8221 CGGGATGGGC GGAGTTAGGG GCGGGACTAT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     8281 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CTGGTTGCTG ACTAATTGAG 
     8341 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCCTAACTG 
     8401 ACACACATTC CACAGCTGGT TCTTTCCGCC TCAGGACTCT TCCTTTTTCA ATATTATTGA 
     8461 AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT 
     8521 AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGACGC GCCCTGTAGC 
     8581 GGCGCATTAA GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG TGACCGCTAC ACTTGCCAGC 
     8641 GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC TCGCCACGTT CGCCGGCTTT 
     8701 CCCCGTCAAG CTCTAAATCG GGGGCTCCCT TTAGGGTTCC GATTTAGTGC TTTACGGCAC 
     8761 CTCGACCCCA AAAAACTTGA TTAGGGTGAT GGTTCACGTA GTGGGCCATC GCCCTGATAG 
     8821 ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA ATAGTGGACT CTTGTTCCAA 
     8881 ACTGGAACAA CACTCAACCC TATCTCGGTC TATTCTTTTG ATTTATAAGG GATTTTGCCG 
     8941 ATTTCGGCCT ATTGGTTAAA AAATGAGCTG ATTTAACAAA AATTTAACGC GAATTTT
//