LOCUS       RR214841                7368 bp    DNA     circular  SYN 
DEFINITION  Uhrf1 (Myc-DDK-tagged ORF) - Rat ubiquitin-like with PHD and ring finger domains 1 (Uhrf1), (10 ug)
ACCESSION   NM_001008882
VERSION     NM_001008882.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3525..3616 
                     /label="Myc-DDK"  
     misc_feature    3646..4231 
                     /label="PolyA signal"  
     misc_feature    3703..3722 
                     /label="Restriction Enzyme"  
     misc_feature    4507..5179 
                     /label="ColE1"  
     misc_feature    5609..6403 
                     /label="Kan/Neo"  
     misc_feature    6438..6740 
                     /label="SV40 ori"  
     misc_feature    6826..7303 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3507..3513 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3507 
                     /label="Uhrf1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAGTCAGGA ATCTTTGGCG AGGGAGGATC CCTTCCTCCT CTCCCGGTAT 
     1081 TGCTACGGGG TTTACAAGAC CTGGGCCTTC TTGGTAAACT AGATATAGCC CGCAGGGCCA 
     1141 CACTGACTGG GATCTCCCTC TGTTTTTCAG TATTCCTTCA GCACATCGGC ATCATGTGGA 
     1201 TCCAGGTTCG AACTATGGAT GGGAAGGAGA CCCACACCGT GAACTCTCTA TCCCGGTTGA 
     1261 CCAAGGTGCA GGAGCTGAGG AAAAAGATTG AGGAGCTGTT TCACGTGGAA CCCCAATTGC 
     1321 AGAGACTCTT TTACAGGGGC AAACAGATGG AGGATGGTCA CACACTCTTC GATTATGACG 
     1381 TGCGCCTCAA TGACACAATC CAGCTGCTGG TGCGCCAGAG TCTGGCCCTA CCTCTCAGTA 
     1441 CGAAAGAACG GGACTCGGAG CTCTCAGACT CTGACTCTGG CTATGGTGTG GGTCACAGTG 
     1501 AATCAGACAA GTCGTCCACG CATGGTGAAG GGACAGCTGA TGGGGATGAC AAGACTGTGT 
     1561 GGGAGGACAC TGACCTAGGA CTGTACAAGG TTAATGAGTA CGTGGACGTG CGTGACAATA 
     1621 TCTTTGGTGC ATGGTTTGAG GCCCAGGTGG TCCAGGTACA GAAGAAAGCC CTATCTGAGG 
     1681 AAGAGCCCTG TAGCTCCAGT GCCATTATGG CCCCAGAGGA TGACATCATG TACCACATCA 
     1741 AGTATGATGA CTATCCAGAG CATGGAGTGG ACATTGTCAA AGCCAAGAAT GTCCGTGCTC 
     1801 GCGCTCGCAC TGTGATCCCC TGGGAGGACC TGGAGGTGGG TCAGGTGGTC ATGGCCAACT 
     1861 ACAACGTGGA CTATCCCAGA AAACGTGGCT TCTGGTATGA TGTTGAGATT TGTAGGAAGC 
     1921 GCCAAACCAG GACGGCACGT GAACTGTATG GCAACGTCAT GCTTTTGAAT GATTCTCAGC 
     1981 TCAACAACTG TCGGATCATA TTTGTGGATG AAGTCTTGAA GATTGAGCTC CCTAATGAGA 
     2041 GGAGCCCCTT GATTGGTAGC CCCTCGAGAC GGAAGAGCGG CCCATCCTGC CAGTACTGCA 
     2101 AGGATGACGA GAACAAACCG TGTCGCAAGT GTGCCTGCCA TATTTGTGGT GGGCGTGAGG 
     2161 CTCCCGAGAA ACAAGTGTTG TGTGATGAGT GTGACATGGC CTTCCACCTG TACTGCCTGC 
     2221 AGCCACCGCT CACCTGTGTC CCCCCTGAGC CAGAGTGGTA CTGCCCCAGC TGCCGAACTG 
     2281 ACTCCAGTGA GGTGGTACAA GCAGGGGAGA AACTGAAGAA AAGCAAGAAG AAGGCAAAGA 
     2341 TGGCGTCAGC CACTTCCTCC TCCCGACGGG ACTGGGGCAA GGGCATGGCA TGTGTGGGCC 
     2401 GCACCACAGA GTGTACTATT GTACCGGCCA ACCACTTCGG GCCCATCCCT GGTGTCCCCG 
     2461 TGGGCACCAT GTGGCGCTTC AGAGTCCAGG TCAGTGAGTC CGGTGTGCAT CGGCCACATG 
     2521 TGGCGGGTAT TCATGGTCGG AGCAACGACG GTGCCTACTC ACTGGTGCTG GCTGGTGGCT 
     2581 ATGAGGATGA TGTGGATAAT GGCAATTTCT TCACCTACAC GGGCAGTGGT GGCCGAGACC 
     2641 TCTCTGGCAA CAAGCGTACA GCGGGACAGT CCTCTGACCA GAAACTCACT AATAACAACA 
     2701 GGGCTCTGGC GCTCAATTGC CACTCCCCAA TCAATGAGAA AGGTGCGGAG GCTGAAGACT 
     2761 GGCGCCAGGG TAAGCCGGTG CGTGTGGTCC GGAACATGAA GGGAGGCAAA CATAGCAAGT 
     2821 ACGCACCTGC AGAGGGGAAC CGCTATGATG GCATCTACAA GGTGGTCAAG TACTGGCCGG 
     2881 AGAAGGGGAA ATCTGGCTTC ATTGTGTGGC GCTATCTCCT TCGACGGGAT GACACTGAAC 
     2941 CTGAGCCGTG GACTCGGGAG GGCAAGGACC GCACTCGGCA GCTGGGCCTC ACTATGCAGT 
     3001 ATCCTGAAGG CTACTTGGAG GCCTTGGCTA ACAAGGAGAA GAACAGGAAG CGCCCGGCCA 
     3061 AGGCCTTGGA GCAGGGACCA TCATCTTCCA AGATAGGCAA AAGCAAGCGG AAGTCCACAG 
     3121 GCCCGGCCAC CACAAGCCCC CGTGTCTCCA AGAAGAGCAA GCTGGAGCCC TACACACTTC 
     3181 CATTGCAGCA GGCCAACCTC ATCAAGGAGG ACAAGGGCAA CGCCAAGCTG TGGGATGACG 
     3241 TGCTGAGCTC CCTTCAGGAT GGGCCGTACC AGATCTTCCT GAGCAAGGTG AAGGAGGCTT 
     3301 TCCAGTGCAT CTGTTGCCAG GAGTTGGTGT TCCGGCCAGT CACCACCGTG TGTCAGCACA 
     3361 ACGTTTGTAA GGACTGCCTG GACAGGTCCT TCCGTGCCCA GGTGTTCAGC TGCCCAGCAT 
     3421 GTCGCTATGA CCTGGACCAC AGCTCCCCAA CCCGAGTGAA TCAGCCCTTG CAGACCATTC 
     3481 TCAACCAGCT CTTCCCTGGC TATGGCAGCG GCCGGACGCG TACGCGGCCG CTCGAGCAGA 
     3541 AACTCATCTC AGAAGAGGAT CTGGCAGCAA ATGATATCCT GGATTACAAG GATGACGACG 
     3601 ATAAGGTTTA AACGGCCGGC CGCGGTCATA GCTGTTTCCT GAACAGATCC CGGGTGGCAT 
     3661 CCCTGTGACC CCTCCCCAGT GCCTCTCCTG GCCCTGGAAG TTGCCACTCC AGTGCCCACC 
     3721 AGCCTTGTCC TAATAAAATT AAGTTGCATC ATTTTGTCTG ACTAGGTGTC CTTCTATAAT 
     3781 ATTATGGGGT GGAGGGGGGT GGTATGGAGC AAGGGGCAAG TTGGGAAGAC AACCTGTAGG 
     3841 GCCTGCGGGG TCTATTGGGA ACCAAGCTGG AGTGCAGTGG CACAATCTTG GCTCACTGCA 
     3901 ATCTCCGCCT CCTGGGTTCA AGCGATTCTC CTGCCTCAGC CTCCCGAGTT GTTGGGATTC 
     3961 CAGGCATGCA TGACCAGGCT CAGCTAATTT TTGTTTTTTT GGTAGAGACG GGGTTTCACC 
     4021 ATATTGGCCA GGCTGGTCTC CAACTCCTAA TCTCAGGTGA TCTACCCACC TTGGCCTCCC 
     4081 AAATTGCTGG GATTACAGGC GTGAACCACT GCTCCCTTCC CTGTCCTTCT GATTTTAAAA 
     4141 TAACTATACC AGCAGGAGGA CGTCCAGACA CAGCATAGGC TACCTGGCCA TGCCCAACCG 
     4201 GTGGGACATT TGAGTTGCTT GCTTGGCACT GTCCTCTCAT GCGTTGGGTC CACTCAGTAG 
     4261 ATGCCTGTTG AATTGGGTAC GCGGCCAGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT 
     4321 AATACGGTTA TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA 
     4381 GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC 
     4441 CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT 
     4501 ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT 
     4561 GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG 
     4621 CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA 
     4681 CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA 
     4741 CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC 
     4801 GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG 
     4861 AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG 
     4921 TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA 
     4981 GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC 
     5041 TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG 
     5101 GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA 
     5161 TGAGTAACCT GAGGCTATGG CAGGGCCTGC CGCCCCGACG TTGGCTGCGA GCCCTGGGCC 
     5221 TTCACCCGAA CTTGGGGGGT GGGGTGGGGA AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA 
     5281 ATGGGGTCTC GGTGGGGTAT CGACAGAGTG CCAGCCCTGG GACCGAACCC CGCGTTTATG 
     5341 AACAAACGAC CCAACACCGT GCGTTTTATT CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT 
     5401 TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA 
     5461 CTCCAGCATG AGATCCCCGC GCTGGAGGAT CATCCAGCCG GCGTCCCGGA AAACGATTCC 
     5521 GAAGCCCAAC CTTTCATAGA AGGCGGCGGT GGAATCGAAA TCTCGTGATG GCAGGTTGGG 
     5581 CGTCGCTTGG TCGGTCATTT CGAACCCCAG AGTCCCGCTC AGAAGAACTC GTCAAGAAGG 
     5641 CGATAGAAGG CGATGCGCTG CGAATCGGGA GCGGCGATAC CGTAAAGCAC GAGGAAGCGG 
     5701 TCAGCCCATT CGCCGCCAAG CTCTTCAGCA ATATCACGGG TAGCCAACGC TATGTCCTGA 
     5761 TAGCGATCCG CCACACCCAG CCGGCCACAG TCGATGAATC CAGAAAAGCG GCCATTTTCC 
     5821 ACCATGATAT TCGGCAAGCA GGCATCGCCA TGGGTCACGA CGAGATCCTC GCCGTCGGGC 
     5881 ATGCTCGCCT TGAGCCTGGC GAACAGTTCG GCTGGCGCGA GCCCCTGATG CTCTTCGTCC 
     5941 AGATCATCCT GATCGACAAG ACCGGCTTCC ATCCGAGTAC GTGCTCGCTC GATGCGATGT 
     6001 TTCGCTTGGT GGTCGAATGG GCAGGTAGCC GGATCAAGCG TATGCAGCCG CCGCATTGCA 
     6061 TCAGCCATGA TGGATACTTT CTCGGCAGGA GCAAGGTGAG ATGACAGGAG ATCCTGCCCC 
     6121 GGCACTTCGC CCAATAGCAG CCAGTCCCTT CCCGCTTCAG TGACAACGTC GAGCACAGCT 
     6181 GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC GATAGCCGCG CTGCCTCGTC TTGCAGTTCA 
     6241 TTCAGGGCAC CGGACAGGTC GGTCTTGACA AAAAGAACCG GGCGCCCCTG CGCTGACAGC 
     6301 CGGAACACGG CGGCATCAGA GCAGCCGATT GTCTGTTGTG CCCAGTCATA GCCGAATAGC 
     6361 CTCTCCACCC AAGCGGCCGG AGAACCTGCG TGCAATCCAT CTTGTTCAAT CATGCGAAAC 
     6421 GATCCTCATC CTGTCTCTTG ATCGATCTTT GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC 
     6481 TCACTACTTC TGGAATAGCT CAGAGGCCGA GGCGGCCTCG GCCTCTGCAT AAATAAAAAA 
     6541 AATTAGTCAG CCATGGGGCG GAGAATGGGC GGAACTGGGC GGAGTTAGGG GCGGGATGGG 
     6601 CGGAGTTAGG GGCGGGACTA TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     6661 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCTGGTTGCT GACTAATTGA GATGCATGCT 
     6721 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCCTAACT GACACACATT 
     6781 CCACAGCTGG TTCTTTCCGC CTCAGGACTC TTCCTTTTTC AATATTATTG AAGCATTTAT 
     6841 CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA 
     6901 GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTGACG CGCCCTGTAG CGGCGCATTA 
     6961 AGCGCGGCGG GTGTGGTGGT TACGCGCAGC GTGACCGCTA CACTTGCCAG CGCCCTAGCG 
     7021 CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT CTCGCCACGT TCGCCGGCTT TCCCCGTCAA 
     7081 GCTCTAAATC GGGGGCTCCC TTTAGGGTTC CGATTTAGTG CTTTACGGCA CCTCGACCCC 
     7141 AAAAAACTTG ATTAGGGTGA TGGTTCACGT AGTGGGCCAT CGCCCTGATA GACGGTTTTT 
     7201 CGCCCTTTGA CGTTGGAGTC CACGTTCTTT AATAGTGGAC TCTTGTTCCA AACTGGAACA 
     7261 ACACTCAACC CTATCTCGGT CTATTCTTTT GATTTATAAG GGATTTTGCC GATTTCGGCC 
     7321 TATTGGTTAA AAAATGAGCT GATTTAACAA AAATTTAACG CGAATTTT
//