LOCUS       RR214818                7095 bp    DNA     circular  SYN 
DEFINITION  Gys1 (Myc-DDK-tagged ORF) - Rat glycogen synthase 1, muscle (Gys1), (10 ug)
ACCESSION   NM_001109615
VERSION     NM_001109615.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3252..3343 
                     /label="Myc-DDK"  
     misc_feature    3373..3958 
                     /label="PolyA signal"  
     misc_feature    3430..3449 
                     /label="Restriction Enzyme"  
     misc_feature    4234..4906 
                     /label="ColE1"  
     misc_feature    5336..6130 
                     /label="Kan/Neo"  
     misc_feature    6165..6467 
                     /label="SV40 ori"  
     misc_feature    6553..7030 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3234..3240 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3234 
                     /label="Gys1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCCTCTCAGC CGCAGTCTCT CTATGTCCTC GCTTCCAGGA TTGGAAGACT 
     1081 GGGAGGATGA ATTCGACCCG GAGAACGCAG TGCTTTTCGA GGTGGCCTGG GAGGTGGCCA 
     1141 ACAAGGTGGG TGGCATCTAC ACTGTGCTGC AGACGAAGGC GAAGGTGACC GGGGATGAAT 
     1201 GGGGTGACAA CTACTATCTG GTGGGACCAT ACACGGAGCA GGGTGTGAGG ACGCAGGTGG 
     1261 AGCTCCTGGA GCCCCCAACT CCTGAACTGA AGAGGACTCT GGATTCCATG AACAGCAAGG 
     1321 GCTGTAAGGT GTATTTTGGG CGTTGGCTGA TCGAGGGGGG ACCCCTAGTG GTGCTCCTGG 
     1381 ATGTAGGGGC CTCAGCCTGG GCCCTGGAGC GCTGGAAGGG TGAACTTTGG GACACCTGCA 
     1441 ACATCGGGGT ACCCTGGTAC GACCGTGAGG CCAATGATGC CGTCCTGTTT GGTTTCCTCA 
     1501 CCACCTGGTT CCTGGGTGAG TTCCTGGCCC AGAATGAAGA GAAGCCATAT GTGGTCGCCC 
     1561 ACTTCCATGA ATGGTTGGCA GGCGTTGGTC TGTGTCTGTG CCGAGCCCGG CGTTTGCCGG 
     1621 TGGCAACCAT CTTCACCACT CACGCCACGC TGCTGGGTCG CTACCTATGT GCTGGTGCCG 
     1681 TGGACTTCTA CAACAACCTG GAGAATTTCA ATGTAGACAA GGAAGCAGGC GAGAGGCAGA 
     1741 TCTATCACCG GTACTGCATG GAGCGGGCAG CAGCCCACTG TGCCCATGTC TTCACTACAG 
     1801 TGTCCCAGAT CACCGCGATT GAGGCTCAAC ACCTCCTCAA GAGGAAACCA GATATTGTGA 
     1861 CCCCCAATGG ACTAAATGTG AAGAAGTTCT CTGCTATGCA CGAATTCCAG AACCTTCACG 
     1921 CTCAGAGCAA AGCACGAATC CAGGAGTTTG TGCGTGGCCA TTTTTATGGG CACCTGGACT 
     1981 TCAACCTAGA CAAGACTTTG TATTTCTTTA TCGCTGGACG CTATGAGTTT TCCAACAAGG 
     2041 GAGCTGATAT ATTCCTGGAG GCATTGGCCC GGCTCAACTA CCTGCTCAGA GTGAATGGCA 
     2101 GCGAGCAAAC TGTTGTGGCA TTCTTCATCA TGCCGGCTCG GACCAACAAC TTCAACGTGG 
     2161 AAACCCTGAA GGGCCAAGCC GTGCGCAAAC AGCTATGGGA CACAGCCAAT ACAGTCAAGG 
     2221 AGAAATTTGG GAGGAAGCTC TACGAATCCC TATTAGTGGG TAGCCTCCCC GACATGAACA 
     2281 AGATGCTGGA CAAGGAGGAC TTCACTATGA TGAAGAGAGC CATCTTTGCG ACTCAGCGGC 
     2341 AGTCTTTCCC ACCGGTGTGT ACCCACAACA TGCTGGATGA CTCTTCAGAC CCCATCTTGA 
     2401 CCACCATCCG ACGAATCGGC CTTTTCAACA GTAGTGCGGA CCGTGTGAAG GTGATTTTTC 
     2461 ACCCAGAATT CCTTTCTTCC ACAAGCCCTC TGCTCCCCGT GGACTATGAA GAGTTCGTCC 
     2521 GTGGCTGTCA CCTTGGAGTC TTCCCCTCCT ACTATGAGCC CTGGGGCTAC ACACCAGCGG 
     2581 AGTGCACTGT CATGGGCATC CCGAGCATCT CCACGAACCT CTCCGGCTTT GGCTGCTTTA 
     2641 TGGAGGAGCA CATTGCAGAT CCCTCGGCTT ACGGCATTTA CATTCTGGAT CGGAGGTTCC 
     2701 GAAGCCTGGA TGATTCATGC TCCCAACTCA CCTCCTTCCT GTACAGCTTC TGTCAGCAGA 
     2761 GCCGGCGACA GCGCATCATT CAGCGGAATC GCACAGAGCG ATTGTCAGAC CTGCTGGATT 
     2821 GGAAGTACCT AGGCCGGTAC TACATGTCTG CGCGCCACAT GGCTCTGGCC AAGGCCTTTC 
     2881 CAGACCACTT CACCTATGAG CCCCATGAGG TAGATGCGAC CCAGGGGTAC CGTTACCCAC 
     2941 GGCCGGCCTC CGTGCCACCG TCACCCTCAC TGTCTCGACA CTCCAGCCCA CACCAGAGTG 
     3001 AGGATGAAGA AGAGCCACGG GATGGACCCC TGAGGGAAGA CAGTGAGCGT TATGATGAGG 
     3061 AAGAAGAGGC TGCCAAGGAC CGCCGCAACA TCCGGGCACC CGAGTGGCCA CGCCGGGCCT 
     3121 CCTGCTCCTC CTCCACAGGC GGCAGCAAGA GAAGCAACTC AGTGGACACT GGGCCCTCCA 
     3181 GCTCACTCAG TACACCCACT GAGCCCCTGA GTCCTACCAG TTCCCTGGGC GAGGAGCGCA 
     3241 ACACGCGTAC GCGGCCGCTC GAGCAGAAAC TCATCTCAGA AGAGGATCTG GCAGCAAATG 
     3301 ATATCCTGGA TTACAAGGAT GACGACGATA AGGTTTAAAC GGCCGGCCGC GGTCATAGCT 
     3361 GTTTCCTGAA CAGATCCCGG GTGGCATCCC TGTGACCCCT CCCCAGTGCC TCTCCTGGCC 
     3421 CTGGAAGTTG CCACTCCAGT GCCCACCAGC CTTGTCCTAA TAAAATTAAG TTGCATCATT 
     3481 TTGTCTGACT AGGTGTCCTT CTATAATATT ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG 
     3541 GGGCAAGTTG GGAAGACAAC CTGTAGGGCC TGCGGGGTCT ATTGGGAACC AAGCTGGAGT 
     3601 GCAGTGGCAC AATCTTGGCT CACTGCAATC TCCGCCTCCT GGGTTCAAGC GATTCTCCTG 
     3661 CCTCAGCCTC CCGAGTTGTT GGGATTCCAG GCATGCATGA CCAGGCTCAG CTAATTTTTG 
     3721 TTTTTTTGGT AGAGACGGGG TTTCACCATA TTGGCCAGGC TGGTCTCCAA CTCCTAATCT 
     3781 CAGGTGATCT ACCCACCTTG GCCTCCCAAA TTGCTGGGAT TACAGGCGTG AACCACTGCT 
     3841 CCCTTCCCTG TCCTTCTGAT TTTAAAATAA CTATACCAGC AGGAGGACGT CCAGACACAG 
     3901 CATAGGCTAC CTGGCCATGC CCAACCGGTG GGACATTTGA GTTGCTTGCT TGGCACTGTC 
     3961 CTCTCATGCG TTGGGTCCAC TCAGTAGATG CCTGTTGAAT TGGGTACGCG GCCAGCGGCG 
     4021 AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG GGGATAACGC 
     4081 AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT 
     4141 GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG 
     4201 TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC 
     4261 CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC 
     4321 TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT 
     4381 CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT 
     4441 ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC 
     4501 AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA 
     4561 GTGGTGGCCT AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA 
     4621 GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG 
     4681 TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA 
     4741 AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG 
     4801 GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG 
     4861 AAGTTTTAAA TCAATCTAAA GTATATATGA GTAACCTGAG GCTATGGCAG GGCCTGCCGC 
     4921 CCCGACGTTG GCTGCGAGCC CTGGGCCTTC ACCCGAACTT GGGGGGTGGG GTGGGGAAAA 
     4981 GGAAGAAACG CGGGCGTATT GGCCCCAATG GGGTCTCGGT GGGGTATCGA CAGAGTGCCA 
     5041 GCCCTGGGAC CGAACCCCGC GTTTATGAAC AAACGACCCA ACACCGTGCG TTTTATTCTG 
     5101 TCTTTTTATT GCCGTCATAG CGCGGGTTCC TTCCGGTATT GTCTCCTTCC GTGTTTCAGT 
     5161 TAGCCTCCCC CTAGGGTGGG CGAAGAACTC CAGCATGAGA TCCCCGCGCT GGAGGATCAT 
     5221 CCAGCCGGCG TCCCGGAAAA CGATTCCGAA GCCCAACCTT TCATAGAAGG CGGCGGTGGA 
     5281 ATCGAAATCT CGTGATGGCA GGTTGGGCGT CGCTTGGTCG GTCATTTCGA ACCCCAGAGT 
     5341 CCCGCTCAGA AGAACTCGTC AAGAAGGCGA TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG 
     5401 GCGATACCGT AAAGCACGAG GAAGCGGTCA GCCCATTCGC CGCCAAGCTC TTCAGCAATA 
     5461 TCACGGGTAG CCAACGCTAT GTCCTGATAG CGATCCGCCA CACCCAGCCG GCCACAGTCG 
     5521 ATGAATCCAG AAAAGCGGCC ATTTTCCACC ATGATATTCG GCAAGCAGGC ATCGCCATGG 
     5581 GTCACGACGA GATCCTCGCC GTCGGGCATG CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT 
     5641 GGCGCGAGCC CCTGATGCTC TTCGTCCAGA TCATCCTGAT CGACAAGACC GGCTTCCATC 
     5701 CGAGTACGTG CTCGCTCGAT GCGATGTTTC GCTTGGTGGT CGAATGGGCA GGTAGCCGGA 
     5761 TCAAGCGTAT GCAGCCGCCG CATTGCATCA GCCATGATGG ATACTTTCTC GGCAGGAGCA 
     5821 AGGTGAGATG ACAGGAGATC CTGCCCCGGC ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC 
     5881 GCTTCAGTGA CAACGTCGAG CACAGCTGCG CAAGGAACGC CCGTCGTGGC CAGCCACGAT 
     5941 AGCCGCGCTG CCTCGTCTTG CAGTTCATTC AGGGCACCGG ACAGGTCGGT CTTGACAAAA 
     6001 AGAACCGGGC GCCCCTGCGC TGACAGCCGG AACACGGCGG CATCAGAGCA GCCGATTGTC 
     6061 TGTTGTGCCC AGTCATAGCC GAATAGCCTC TCCACCCAAG CGGCCGGAGA ACCTGCGTGC 
     6121 AATCCATCTT GTTCAATCAT GCGAAACGAT CCTCATCCTG TCTCTTGATC GATCTTTGCA 
     6181 AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA CTACTTCTGG AATAGCTCAG AGGCCGAGGC 
     6241 GGCCTCGGCC TCTGCATAAA TAAAAAAAAT TAGTCAGCCA TGGGGCGGAG AATGGGCGGA 
     6301 ACTGGGCGGA GTTAGGGGCG GGATGGGCGG AGTTAGGGGC GGGACTATGG TTGCTGACTA 
     6361 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCT 
     6421 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     6481 CTTTCCACAC CCTAACTGAC ACACATTCCA CAGCTGGTTC TTTCCGCCTC AGGACTCTTC 
     6541 CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT 
     6601 GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA 
     6661 CCTGACGCGC CCTGTAGCGG CGCATTAAGC GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG 
     6721 ACCGCTACAC TTGCCAGCGC CCTAGCGCCC GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC 
     6781 GCCACGTTCG CCGGCTTTCC CCGTCAAGCT CTAAATCGGG GGCTCCCTTT AGGGTTCCGA 
     6841 TTTAGTGCTT TACGGCACCT CGACCCCAAA AAACTTGATT AGGGTGATGG TTCACGTAGT 
     6901 GGGCCATCGC CCTGATAGAC GGTTTTTCGC CCTTTGACGT TGGAGTCCAC GTTCTTTAAT 
     6961 AGTGGACTCT TGTTCCAAAC TGGAACAACA CTCAACCCTA TCTCGGTCTA TTCTTTTGAT 
     7021 TTATAAGGGA TTTTGCCGAT TTCGGCCTAT TGGTTAAAAA ATGAGCTGAT TTAACAAAAA 
     7081 TTTAACGCGA ATTTT
//