LOCUS       RR214811                7206 bp    DNA     circular  SYN 
DEFINITION  Maged1 (Myc-DDK-tagged ORF) - Rat melanoma antigen, family D, 1 (Maged1), (10 ug)
ACCESSION   NM_053409
VERSION     NM_053409.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3363..3454 
                     /label="Myc-DDK"  
     misc_feature    3484..4069 
                     /label="PolyA signal"  
     misc_feature    3541..3560 
                     /label="Restriction Enzyme"  
     misc_feature    4345..5017 
                     /label="ColE1"  
     misc_feature    5447..6241 
                     /label="Kan/Neo"  
     misc_feature    6276..6578 
                     /label="SV40 ori"  
     misc_feature    6664..7141 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3345..3351 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3345 
                     /label="Maged1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCTCAGAAA CCGGACGGCG GTGCAGGCCT CCGCGGCTTC CAGGCAGAGG 
     1081 CCTCTGTAGA AGACAGCGCC TTGCTTGTGC AGACCTTGAT GGAAGCCATC CAGATCTCCG 
     1141 AGGCTCCGCC CACCAGCCAG GCCACAGCAG CTGCCAGTGG GCCGAATGCT AGTCCCCAGA 
     1201 GTTCACAGCC CCCAACTGCC AATGAGAAGG CTGATACTGA GGTTTCAGCA GCTGCTGCCA 
     1261 GGCCTAAGAC AGGCTTTAAG GCCCAGAATA CCACCACAAA GGGGCCAAAT GATTACTCTC 
     1321 AGGCACGTAA TGCCAAGGAG ATGCCCAAGA ATCAGCCTAA GGTGGCCTTT AAGTCACAGA 
     1381 ATGCCACCTC TAAAGGTCCA CATGCTGCCT CTGATTTTTC CCATGCAGCA TCCACAGGCA 
     1441 AATCAGCAGC TAAAAAGTCT GAAATGGCCT TTAAGGGTCA GAATACCACT ACTAAGGCTG 
     1501 GCCCCAGTGC CACCTACAAT TTCACTCAGT CTCCCAGTGC CAATGAGATG ACCAACAACC 
     1561 AGCCTAAGAC AGCTAAGGCT TGGAATGACA CCACTAAGAT CCCTGGAGCT GATGCCCAGA 
     1621 CCCAGAATGT AAACCAGGCC AAAATGGCTG ACGTAGGGAC CAGTGCAGGT ATCTCTGAAA 
     1681 CTGACGGTGC AGCAGCCCAG ACCTCAGCAG ATGGCTCCCA GGCTCAGAAT GTGGAGTCCC 
     1741 GGACTATAAT TCGGGGCAAG AGGACCCGCA AGATTAATAA CTTGAATGTG GAAGAGAACA 
     1801 GCAATGGGGA CCAAAGGCGT GCCTCGCTGG CTTCCGGGAA CTGGAGGTCT GCTCCGGTTC 
     1861 CAGTAACCAC TCAGAACCCA CCTGGCGCAC CCCCTAATGT GCTGTGGCAG ACACCACTGG 
     1921 CTTGGCAGAA CCCATCAGGC TGGCAAAACC AGACAGCCAG GCAGACCCCA CCAGCACGTC 
     1981 AGAGTCCCCC AGCTAGGCAG ACACCATCAG CTTGGCAGAA CCCCGTTGCA TGGCAGAATC 
     2041 CAGTGATCTG GCCTAACCCA GTGATCTGGC AGAATCCAGT GATCTGGCCA AACCCCATTG 
     2101 TCTGGCCTGG CCCAATTGTC TGGCCAAACC CAATGGCCTG GCAGAGTACA CCTGGATGGC 
     2161 AGAGCCCACC CAGTTGGCAG GCTCCACCTA GTTGGCAGAG CCCTCAAGAT TGGCAAGGCC 
     2221 CTCCAGATTG GCAGTTACCA CCTGACTGGT CAATGCCTCC TGACTGGTCC TTTCCCTCTG 
     2281 ACTGGCCTTT TCCACCTGAC TGGATCCCTG CCGACTGGCC AATTCCACCC GACTGGCAGA 
     2341 ACTTACGACC CTCACCTAAT CTGAGATCCT CCCCCAACTC TCGTGCCTCA CAGAACCAGG 
     2401 GTCCTCCACA GCCCCGAGAT GTGGCCCTTC TTCAGGAAAG AGCAAATAAG CTGGTCAAGT 
     2461 ACTTGATGCT TAAAGACTAC ACGAAGGTGC CCATCAAGCG CTCAGAAATG CTGAGAGATA 
     2521 TCATCCGAGA ATACACTGAT GTTTATCCAG AAATCATTGA ACGCGCATGC TTTGTCCTGG 
     2581 AGAAGAAATT TGGAATCCAG CTCAAGGAAA TCGACAAAGA AGAGCATCTG TATATCCTCA 
     2641 TCAGTACCCC TGAATCCCTG GCTGGCATAC TGGGAACGAC CAAAGACACA CCGAAGCTAG 
     2701 GCCTCCTCTT AGTGATTCTG GGCATTATCT TCATGAATGG CAACCGTGCC ACTGAGGCCG 
     2761 TCCTCTGGGA AGCACTGCGC AAGATGGGAC TACGTCCTGG GGTCAGACAT CCCCTCCTTG 
     2821 GCGATCTGAG GAAACTTCTT ACTTACGAGT TTGTAAAGCA GAAATACCTG GACTACAGAC 
     2881 GAGTGCCCAA CAGCAACCCT CCTGAGTATG AGTTCCTCTG GGGCCTCCGC TCCTACCATG 
     2941 AGACTAGCAA GATGAAAGTG CTGAGATTCA TTGCAGAGGT TCAGAAGAGA GACCCTCGTG 
     3001 ACTGGACTGC ACAGTTCATG GAAGCTGCAG ATGAAGCCTT GGATGCTCTG GATGCTGCTG 
     3061 CAGCTGAGGC AGAGGCCCGG GCCGAAGCAA GAAACCGCAT GGGGATTGGA GACGAGGCTG 
     3121 TGTCTGGGCC CTGGAGCTGG GATGACATTG AGTTTGAGCT GCTGACCTGG GATGAGGAAG 
     3181 GAGATTTTGG AGATCCTTGG TCCAGGATCC CCTTTACCTT CTGGGCCAGA TACCACCAGA 
     3241 ATGCCCGCTC CAGGTTTCCC CAGGCCTTTA CCGGCCCCAT CATTGGCCCC AGCGGCACTG 
     3301 CCACCGCCAA CTTCGCCGCC AACTTCGGTG CCATTGGCTT CTTCTGGGTT GAGACGCGTA 
     3361 CGCGGCCGCT CGAGCAGAAA CTCATCTCAG AAGAGGATCT GGCAGCAAAT GATATCCTGG 
     3421 ATTACAAGGA TGACGACGAT AAGGTTTAAA CGGCCGGCCG CGGTCATAGC TGTTTCCTGA 
     3481 ACAGATCCCG GGTGGCATCC CTGTGACCCC TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT 
     3541 GCCACTCCAG TGCCCACCAG CCTTGTCCTA ATAAAATTAA GTTGCATCAT TTTGTCTGAC 
     3601 TAGGTGTCCT TCTATAATAT TATGGGGTGG AGGGGGGTGG TATGGAGCAA GGGGCAAGTT 
     3661 GGGAAGACAA CCTGTAGGGC CTGCGGGGTC TATTGGGAAC CAAGCTGGAG TGCAGTGGCA 
     3721 CAATCTTGGC TCACTGCAAT CTCCGCCTCC TGGGTTCAAG CGATTCTCCT GCCTCAGCCT 
     3781 CCCGAGTTGT TGGGATTCCA GGCATGCATG ACCAGGCTCA GCTAATTTTT GTTTTTTTGG 
     3841 TAGAGACGGG GTTTCACCAT ATTGGCCAGG CTGGTCTCCA ACTCCTAATC TCAGGTGATC 
     3901 TACCCACCTT GGCCTCCCAA ATTGCTGGGA TTACAGGCGT GAACCACTGC TCCCTTCCCT 
     3961 GTCCTTCTGA TTTTAAAATA ACTATACCAG CAGGAGGACG TCCAGACACA GCATAGGCTA 
     4021 CCTGGCCATG CCCAACCGGT GGGACATTTG AGTTGCTTGC TTGGCACTGT CCTCTCATGC 
     4081 GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA TTGGGTACGC GGCCAGCGGC GAGCGGTATC 
     4141 AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA 
     4201 CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT 
     4261 TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG 
     4321 GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG 
     4381 CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG 
     4441 CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC 
     4501 CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA 
     4561 CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG 
     4621 TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC 
     4681 TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC 
     4741 CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG 
     4801 TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT 
     4861 GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT 
     4921 CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA 
     4981 ATCAATCTAA AGTATATATG AGTAACCTGA GGCTATGGCA GGGCCTGCCG CCCCGACGTT 
     5041 GGCTGCGAGC CCTGGGCCTT CACCCGAACT TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC 
     5101 GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG TGGGGTATCG ACAGAGTGCC AGCCCTGGGA 
     5161 CCGAACCCCG CGTTTATGAA CAAACGACCC AACACCGTGC GTTTTATTCT GTCTTTTTAT 
     5221 TGCCGTCATA GCGCGGGTTC CTTCCGGTAT TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC 
     5281 CCTAGGGTGG GCGAAGAACT CCAGCATGAG ATCCCCGCGC TGGAGGATCA TCCAGCCGGC 
     5341 GTCCCGGAAA ACGATTCCGA AGCCCAACCT TTCATAGAAG GCGGCGGTGG AATCGAAATC 
     5401 TCGTGATGGC AGGTTGGGCG TCGCTTGGTC GGTCATTTCG AACCCCAGAG TCCCGCTCAG 
     5461 AAGAACTCGT CAAGAAGGCG ATAGAAGGCG ATGCGCTGCG AATCGGGAGC GGCGATACCG 
     5521 TAAAGCACGA GGAAGCGGTC AGCCCATTCG CCGCCAAGCT CTTCAGCAAT ATCACGGGTA 
     5581 GCCAACGCTA TGTCCTGATA GCGATCCGCC ACACCCAGCC GGCCACAGTC GATGAATCCA 
     5641 GAAAAGCGGC CATTTTCCAC CATGATATTC GGCAAGCAGG CATCGCCATG GGTCACGACG 
     5701 AGATCCTCGC CGTCGGGCAT GCTCGCCTTG AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC 
     5761 CCCTGATGCT CTTCGTCCAG ATCATCCTGA TCGACAAGAC CGGCTTCCAT CCGAGTACGT 
     5821 GCTCGCTCGA TGCGATGTTT CGCTTGGTGG TCGAATGGGC AGGTAGCCGG ATCAAGCGTA 
     5881 TGCAGCCGCC GCATTGCATC AGCCATGATG GATACTTTCT CGGCAGGAGC AAGGTGAGAT 
     5941 GACAGGAGAT CCTGCCCCGG CACTTCGCCC AATAGCAGCC AGTCCCTTCC CGCTTCAGTG 
     6001 ACAACGTCGA GCACAGCTGC GCAAGGAACG CCCGTCGTGG CCAGCCACGA TAGCCGCGCT 
     6061 GCCTCGTCTT GCAGTTCATT CAGGGCACCG GACAGGTCGG TCTTGACAAA AAGAACCGGG 
     6121 CGCCCCTGCG CTGACAGCCG GAACACGGCG GCATCAGAGC AGCCGATTGT CTGTTGTGCC 
     6181 CAGTCATAGC CGAATAGCCT CTCCACCCAA GCGGCCGGAG AACCTGCGTG CAATCCATCT 
     6241 TGTTCAATCA TGCGAAACGA TCCTCATCCT GTCTCTTGAT CGATCTTTGC AAAAGCCTAG 
     6301 GCCTCCAAAA AAGCCTCCTC ACTACTTCTG GAATAGCTCA GAGGCCGAGG CGGCCTCGGC 
     6361 CTCTGCATAA ATAAAAAAAA TTAGTCAGCC ATGGGGCGGA GAATGGGCGG AACTGGGCGG 
     6421 AGTTAGGGGC GGGATGGGCG GAGTTAGGGG CGGGACTATG GTTGCTGACT AATTGAGATG 
     6481 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC TGGTTGCTGA 
     6541 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     6601 CCCTAACTGA CACACATTCC ACAGCTGGTT CTTTCCGCCT CAGGACTCTT CCTTTTTCAA 
     6661 TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT 
     6721 TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC ACCTGACGCG 
     6781 CCCTGTAGCG GCGCATTAAG CGCGGCGGGT GTGGTGGTTA CGCGCAGCGT GACCGCTACA 
     6841 CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC GCTTTCTTCC CTTCCTTTCT CGCCACGTTC 
     6901 GCCGGCTTTC CCCGTCAAGC TCTAAATCGG GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT 
     6961 TTACGGCACC TCGACCCCAA AAAACTTGAT TAGGGTGATG GTTCACGTAG TGGGCCATCG 
     7021 CCCTGATAGA CGGTTTTTCG CCCTTTGACG TTGGAGTCCA CGTTCTTTAA TAGTGGACTC 
     7081 TTGTTCCAAA CTGGAACAAC ACTCAACCCT ATCTCGGTCT ATTCTTTTGA TTTATAAGGG 
     7141 ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA AATGAGCTGA TTTAACAAAA ATTTAACGCG 
     7201 AATTTT
//