LOCUS       RR214766                8481 bp    DNA     circular  SYN 
DEFINITION  Nek1 (Myc-DDK-tagged ORF) - Rat NIMA (never in mitosis gene a)-related expressed kinase 1 (Nek1), (10 ug)
ACCESSION   NM_001106082
VERSION     NM_001106082.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4638..4729 
                     /label="Myc-DDK"  
     misc_feature    4759..5344 
                     /label="PolyA signal"  
     misc_feature    4816..4835 
                     /label="Restriction Enzyme"  
     misc_feature    5620..6292 
                     /label="ColE1"  
     misc_feature    6722..7516 
                     /label="Kan/Neo"  
     misc_feature    7551..7853 
                     /label="SV40 ori"  
     misc_feature    7939..8416 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4620..4626 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4620 
                     /label="Nek1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAGAAGTAT GTTAGACTAC AGAAGATTGG AGAAGGTTCA TTTGGAAAAG 
     1081 CTGTTCTTGT TAAATCTACA GAGGATGGCA GACATTATGT CATCAAGGAA ATTAACATCT 
     1141 CAAGAATGTC CGATAAAGAA AGAGAAGAAT CAAGGAGAGA GGTCGCAGTG TTGGCAAACA 
     1201 TGAAGCATCC AAATATTGTC CAATACAAAG AATCATTCGA AGAAAATGGC TCTCTCTACA 
     1261 TAGTAATGGA TTACTGTGAA GGAGGTGATC TGTTTAAACG AATAAATGCT CAGAAAGGCA 
     1321 CTTTGTTTCA AGAAGATCAG ATTTTGGACT GGTTTGTTCA GATATGTTTG GCTTTGAAGC 
     1381 ATGTACACGA TAGAAAAATT CTTCACCGAG ACATAAAGTC ACAGAACATA TTTCTAACCA 
     1441 AAGATGGGAC AGTGCAGCTT GGAGATTTTG GAATTGCTCG AGTTCTCAAT AGTACTGTAG 
     1501 AGCTGGCTCG AACTTGCATA GGGACTCCAT ACTACTTGTC ACCTGAAATC TGTGAAAACA 
     1561 AGCCTTACAA CAATAAAAGT GACATTTGGG CTTTGGGCTG TGTCCTTTAT GAGTTGTGTA 
     1621 CACTTAAACA TGCATTTGAA GCTGGAAACA TGAAAAACCT GGTACTGAAG ATAATATCTG 
     1681 GATCCTTTCC TCCAGTGTCT CTACATTATT CCTATGATCT CCGCAGCTTG CTCTCTCAGC 
     1741 TGTTTAAAAG AAATCCTAGG GATAGACCAT CAGTCAACTC CATATTGGAG AAAGGCTTTA 
     1801 TAGCTAAACG AATCGAAAAG TTTCTCTCAC CTCAGCTTAT TGCAGAAGAA TTTTGTCTGA 
     1861 AAACAGTTTC AAAGTTTGGA CCACAGCCTA TCCCAGGTAA AAGACCAGCA TCAGGACAAG 
     1921 GTGTCAGTTC TTTTGTCCCT GCTCAGAAAA TCACAAAGCC TTCTGCTAAA TATGGAGTGC 
     1981 CTATAACATA TAAGAAGTAC GGAGATAAAA AGTTACTTGA AAAAAAACCA CCCCCAAAGC 
     2041 ATAAGCAGGC CCATCAGATT CCAGTGAAGA AAATGAATTC TGGAGAAGAA AGGAAGAAAA 
     2101 TGTCTGAGGA AGCAGCAAAA AAACGAAGGT TGGAATTTAT TGAGAAAGAA AAGAAGCAAA 
     2161 AGGATCAGAT CAGGTTCCTG AAGGCTGAGC AGCTGAAGAG GCAAGAGAAG CAGCGGTTGG 
     2221 AGAGGATAAA TAGGGCCAGG GAACAAGGAT GGAGGAATGT TTTAAGGGCT GGTGGAAGCG 
     2281 GTGAAGTAAA GGCTTCCTTT TTTGGCAGTG GAGGAGCTGT GTCTCCATCA CCTTGTTCTC 
     2341 CTCGGGGCCA GTATGAACAT TACCATGCCA TCTTTGACCA AATGCAGCAG CTAAGAGCAG 
     2401 AAGATAATGA AGCGAGATGG AAAGGGAGAA TATATGGTCG ATGGCTCCCT GAAAGGCAAA 
     2461 AAGGACACCT AGGTGTAGAG AGAGCCAAAC AAGTGGAAGA ATTCCTACAG CGTAAACAAG 
     2521 AAGCTATGCA GAATAAAGCC CGAGCAGAAG GACATGTGGT TTATCTGGCA AGACTGAGGC 
     2581 AAATAAGACT ACAAAATTTT AATGAGCGCC AACAGATTAA AGCCAAACTT CGTGGTGAGA 
     2641 ATAAAGAAGC TGGTGGTACC AAAGGACAAG ACGCAACTGA AGAGGCTGAC ATGAGGCTCA 
     2701 AAAAGATGGA GTCACTTAAG GCCCAAGCAA ATGCACGTGC TGCTGTACTA AAAGAACAGC 
     2761 TAGAGCGAAA AAGAAAGGAA GCTTATGAAA GAGAAAAGAA AGTGTGGGAA GAACATTTGG 
     2821 TGGTGAGGGT AAAAAGCTCA GATGTCCCTC CATCTATGGA GCATCATGAA GCAGGTGGTT 
     2881 CTCCATCACA GCAGCAGATG AAACCTGTTA TTTCTGTGAC TTCAGCTTTG AAAGAAGTGG 
     2941 GCCTGGATGG AAGTTTAACT GAGTCCCAAG AAACTTCAGA AGAAATGGAA AAGAGTAACA 
     3001 ATGCTATTTC AAGTAAGCGA GAAATACTGC GTAGGCTGAA TGAAAATCTT AAAGCTCAAG 
     3061 AGGATGAAAA GGAAAAGCAT CATCACGCTG ATTCTTGTGA GACCGTTGGT CACAGAGATG 
     3121 AGAGAGAGTA TGAGAAGGAA AATGCCATTT CCTCTGATCG CAAGAAATGG GAGATGGGAG 
     3181 GTCAGCTTGT GATTCCTCTG GATGAAGTGA CACTGGATGC ATCCTTCTCT GCTTCTGAAA 
     3241 AACATACTGT GGGAGAGGTT ATTAAGTTAG ATTCTAGTGG CTCTCCAAGA AAAGTCTGGG 
     3301 GGAAAAGCCC TACAGATTCT GTGCTGAAGA TACTTGGAGA AGCTGAATTA CAGCTACAGA 
     3361 CAGAACTACT AGAAAACACG CCTTTTAAAA GTGAGGTTTG TGTTGAAGAG GAGAACTACA 
     3421 AACCCCTACT CACTGGAGAA GAGAATCTGC AGTGTATTTC AAAAGAAGTA AACCCATCAG 
     3481 CCACTGTTGA TACTGAAACA AACAGTCCAA AGTTTGCTAA GGTGTCTCCA GCAATGTCCG 
     3541 AAGGAAATGT GGAAGAACCT GATGATTTGG AAACAGAAGT TCTACAAGAG CCAAGTGCCA 
     3601 TACCCTCAGA TGGGAGTTTG CCGCCTGTTG TTAATGAGGT GTGGACCAGA GCTAAGGCAA 
     3661 CTGGGTTGGA GGATAGCATT GCTGTGCAGC AGAATGACGT TTGTGAAGAG CGAATTCCAG 
     3721 GGACTGTGGA CCAGTCCTGT AAGGATCATA TAGAACCTGC AGGAAACGAT TCTCCGCAGT 
     3781 CTGGCTGTGA TGAAGAGAAG TCAGTACAGC CAGAATTGTT TTTCCAGAAA GTGGTTCACT 
     3841 CCAAGGACTT GAACTTAGTT CAGTCAGCTC ATCGTTCCCC AGAAGAAGCA ATTCCAATTC 
     3901 GATCTCACTC TGATTCTCCA CCAAAAGCTA AAAGCAAGAA TTCCCTACTG ATTGGACTTT 
     3961 CCACTGGTCT GTTTGATGCA AACAACCCAA AGATGCTGAG GACCTGTTCA CTTCCAGACC 
     4021 TTTCCAAGCT CTTCAGAACC CTAATGGATG TTCCCACTGT GGGAGACGTT CATCAAGACA 
     4081 GTCTTGAAAT AGAGGAGCTT GAGGATGAAC ATATTAAAGA AGGGCCCTCG GATTGCGAGG 
     4141 ACATTGTATT TGAAGAAACT GACACAGATT TACAGGAGCT TCAGGCCTCA ATGGAGCAGC 
     4201 TGCTTAGGGA GCAACCCGGT GACGAATACA GTGAGGAGGA AGAGTCTGTC TTAAAAAGCA 
     4261 GCAATGTGGA GCGGGCAGCA AGAGGGACAG ATGTCCCAGA TGAGGAGGAC AACCCCAGCA 
     4321 GTGAAAGCGC CCTAAACGAG GAATGGCACT CAGATAACAG TGATGCTGAG ACTACTAGTG 
     4381 AGTGTGAATA TGACAGTGTC TTTAACCATT TAGAGGAACT AAGACTTCAC TTGGAACAAG 
     4441 AAATGGGCTT TGAAAAGTTC TTTGAGGTCT ATGAGAAAGT AAAGGCTATT CATGAGGATG 
     4501 AAGATGAAAA TATTGAAATT TCTTCAACCA TAGTCGAGAA CATCTTGGGC ACTGAGCATC 
     4561 AGCACCTTTA TGCCAAGATT CTGCATCTAG TCATGGCAGA CGGAGCCTAT CAGGAAGATA 
     4621 ATGATGAAAC GCGTACGCGG CCGCTCGAGC AGAAACTCAT CTCAGAAGAG GATCTGGCAG 
     4681 CAAATGATAT CCTGGATTAC AAGGATGACG ACGATAAGGT TTAAACGGCC GGCCGCGGTC 
     4741 ATAGCTGTTT CCTGAACAGA TCCCGGGTGG CATCCCTGTG ACCCCTCCCC AGTGCCTCTC 
     4801 CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC ACCAGCCTTG TCCTAATAAA ATTAAGTTGC 
     4861 ATCATTTTGT CTGACTAGGT GTCCTTCTAT AATATTATGG GGTGGAGGGG GGTGGTATGG 
     4921 AGCAAGGGGC AAGTTGGGAA GACAACCTGT AGGGCCTGCG GGGTCTATTG GGAACCAAGC 
     4981 TGGAGTGCAG TGGCACAATC TTGGCTCACT GCAATCTCCG CCTCCTGGGT TCAAGCGATT 
     5041 CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA TTCCAGGCAT GCATGACCAG GCTCAGCTAA 
     5101 TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC ACCATATTGG CCAGGCTGGT CTCCAACTCC 
     5161 TAATCTCAGG TGATCTACCC ACCTTGGCCT CCCAAATTGC TGGGATTACA GGCGTGAACC 
     5221 ACTGCTCCCT TCCCTGTCCT TCTGATTTTA AAATAACTAT ACCAGCAGGA GGACGTCCAG 
     5281 ACACAGCATA GGCTACCTGG CCATGCCCAA CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC 
     5341 ACTGTCCTCT CATGCGTTGG GTCCACTCAG TAGATGCCTG TTGAATTGGG TACGCGGCCA 
     5401 GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA 
     5461 TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC 
     5521 CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG 
     5581 CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG 
     5641 AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT 
     5701 TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT 
     5761 GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG 
     5821 CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT 
     5881 GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT 
     5941 CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT 
     6001 GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC 
     6061 CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC 
     6121 TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG 
     6181 TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA 
     6241 AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA CCTGAGGCTA TGGCAGGGCC 
     6301 TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG GCCTTCACCC GAACTTGGGG GGTGGGGTGG 
     6361 GGAAAAGGAA GAAACGCGGG CGTATTGGCC CCAATGGGGT CTCGGTGGGG TATCGACAGA 
     6421 GTGCCAGCCC TGGGACCGAA CCCCGCGTTT ATGAACAAAC GACCCAACAC CGTGCGTTTT 
     6481 ATTCTGTCTT TTTATTGCCG TCATAGCGCG GGTTCCTTCC GGTATTGTCT CCTTCCGTGT 
     6541 TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA GAACTCCAGC ATGAGATCCC CGCGCTGGAG 
     6601 GATCATCCAG CCGGCGTCCC GGAAAACGAT TCCGAAGCCC AACCTTTCAT AGAAGGCGGC 
     6661 GGTGGAATCG AAATCTCGTG ATGGCAGGTT GGGCGTCGCT TGGTCGGTCA TTTCGAACCC 
     6721 CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA AGGCGATAGA AGGCGATGCG CTGCGAATCG 
     6781 GGAGCGGCGA TACCGTAAAG CACGAGGAAG CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA 
     6841 GCAATATCAC GGGTAGCCAA CGCTATGTCC TGATAGCGAT CCGCCACACC CAGCCGGCCA 
     6901 CAGTCGATGA ATCCAGAAAA GCGGCCATTT TCCACCATGA TATTCGGCAA GCAGGCATCG 
     6961 CCATGGGTCA CGACGAGATC CTCGCCGTCG GGCATGCTCG CCTTGAGCCT GGCGAACAGT 
     7021 TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG TCCAGATCAT CCTGATCGAC AAGACCGGCT 
     7081 TCCATCCGAG TACGTGCTCG CTCGATGCGA TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA 
     7141 GCCGGATCAA GCGTATGCAG CCGCCGCATT GCATCAGCCA TGATGGATAC TTTCTCGGCA 
     7201 GGAGCAAGGT GAGATGACAG GAGATCCTGC CCCGGCACTT CGCCCAATAG CAGCCAGTCC 
     7261 CTTCCCGCTT CAGTGACAAC GTCGAGCACA GCTGCGCAAG GAACGCCCGT CGTGGCCAGC 
     7321 CACGATAGCC GCGCTGCCTC GTCTTGCAGT TCATTCAGGG CACCGGACAG GTCGGTCTTG 
     7381 ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC AGCCGGAACA CGGCGGCATC AGAGCAGCCG 
     7441 ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT AGCCTCTCCA CCCAAGCGGC CGGAGAACCT 
     7501 GCGTGCAATC CATCTTGTTC AATCATGCGA AACGATCCTC ATCCTGTCTC TTGATCGATC 
     7561 TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC TCCTCACTAC TTCTGGAATA GCTCAGAGGC 
     7621 CGAGGCGGCC TCGGCCTCTG CATAAATAAA AAAAATTAGT CAGCCATGGG GCGGAGAATG 
     7681 GGCGGAACTG GGCGGAGTTA GGGGCGGGAT GGGCGGAGTT AGGGGCGGGA CTATGGTTGC 
     7741 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     7801 ACACCTGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     7861 TGGGGACTTT CCACACCCTA ACTGACACAC ATTCCACAGC TGGTTCTTTC CGCCTCAGGA 
     7921 CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC 
     7981 ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA 
     8041 GTGCCACCTG ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG CGGGTGTGGT GGTTACGCGC 
     8101 AGCGTGACCG CTACACTTGC CAGCGCCCTA GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC 
     8161 TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG 
     8221 TTCCGATTTA GTGCTTTACG GCACCTCGAC CCCAAAAAAC TTGATTAGGG TGATGGTTCA 
     8281 CGTAGTGGGC CATCGCCCTG ATAGACGGTT TTTCGCCCTT TGACGTTGGA GTCCACGTTC 
     8341 TTTAATAGTG GACTCTTGTT CCAAACTGGA ACAACACTCA ACCCTATCTC GGTCTATTCT 
     8401 TTTGATTTAT AAGGGATTTT GCCGATTTCG GCCTATTGGT TAAAAAATGA GCTGATTTAA 
     8461 CAAAAATTTA ACGCGAATTT T
//