LOCUS       RR214763                8475 bp    DNA     circular  SYN 
DEFINITION  Hcn4 (Myc-DDK-tagged ORF) - Rat hyperpolarization activated cyclic nucleotide-gated potassium channel 4 (Hcn4), (10 ug)
ACCESSION   NM_021658
VERSION     NM_021658.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4632..4723 
                     /label="Myc-DDK"  
     misc_feature    4753..5338 
                     /label="PolyA signal"  
     misc_feature    4810..4829 
                     /label="Restriction Enzyme"  
     misc_feature    5614..6286 
                     /label="ColE1"  
     misc_feature    6716..7510 
                     /label="Kan/Neo"  
     misc_feature    7545..7847 
                     /label="SV40 ori"  
     misc_feature    7933..8410 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4614..4620 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4614 
                     /label="Hcn4"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGACAAGCTG CCGCCGTCCA TGCGCAAGCG GCTCTACAGC CTTCCGCAGC 
     1081 AGGTGGGGGC CAAGGCGTGG ATCATGGACG AGGAAGAGGA TGGTGAGGAA GAGGGGGCCG 
     1141 GGGGCCTCCA GGACCCAAGC CGAAGGAGCA TTCGGCTGCG GCCGCTGCCC TCGCCCTCGC 
     1201 CCTCGGTGGC CGCCGGCTGC TCCGAGTCCC GGGGTGCGGC CCTCGGGGCG GCAGACAGCG 
     1261 AGGGGCCGGG CCGCAGCGCC GGCAAGTCCA GCACCAACGG TGACTGCAGG CGCTTCCGCG 
     1321 GGAGTCTGGC CTCGCTGGGC AGCCGGGGCG GCGGCAGTGG TGGAGCAGGG GGCGGCAGCA 
     1381 GTCTCGGGCA CCTGCATGAC TCCGCGGAGG AGCGGCGGCT CATCGCCGCT GAGGGCGATG 
     1441 CGTCCCCCGG CGAGGACAGG ACGCCCCCGG GCCTGGCGAC CGAGCCCGAG CGCCCGGGCG 
     1501 CCGCGGCACA ACCCGCAGCC TCGCCGCCGC CCCAACAGCC GCCGCAGCCG GCCTCCGCCT 
     1561 CCTGCGAGCA GCCCTCGGCG GACACAGCTA TCAAAGTGGA AGGAGGCGCG GCCGCCAGCG 
     1621 ACCAGATCCT CCCTGAGGCC GAGGTGCGCC TGGGCCAGAG CGGCTTCATG CAGCGCCAGT 
     1681 TCGGTGCCAT GCTGCAACCT GGGGTCAACA AATTCTCGCT AAGGATGTTC GGCAGCCAGA 
     1741 AAGCAGTGGA GCGTGAGCAG GAGAGGGTTA AGTCAGCTGG GTTTTGGATT ATCCACCCCT 
     1801 ACAGCGACTT CAGATTTTAC TGGGACCTGA CGATGCTGTT GCTGATGGTG GGGAATCTGA 
     1861 TCATCATCCC TGTGGGCATC ACCTTCTTCA AGGATGAGAA CACCACCCCC TGGATCGTCT 
     1921 TCAACGTGGT GTCAGACACA TTCTTCCTCA TTGACTTGGT CCTCAACTTC CGCACGGGGA 
     1981 TCGTGGTGGA GGACAACACA GAAATCATCC TTGACCCACA GCGGATCAAG ATGAAGTACC 
     2041 TGAAAAGCTG GTTTGTGGTG GACTTCATCT CCTCCATCCC CGTGGACTAC ATCTTCCTTA 
     2101 TAGTGGAGAC TCGCATTGAC TCGGAGGTCT ACAAAACCGC CAGGGCTCTG CGCATTGTCC 
     2161 GCTTCACGAA GATCCTCAGC CTCCTGCGCC TCCTGCGGCT TTCCCGCCTC ATTCGGTACA 
     2221 TTCACCAGTG GGAAGAGATC TTCCACATGA CCTACGACCT GGCCAGTGCC GTGGTACGCA 
     2281 TCGTGAACCT CATTGGCATG ATGCTTCTGC TTTGCCACTG GGATGGCTGC CTGCAGTTCC 
     2341 TGGTGCCCAT GCTGCAGGAC TTCCCCCATG ACTGCTGGGT GTCCATCAAC GGCATGGTGA 
     2401 ATAACTCCTG GGGGAAGCAG TACTCCTATG CCCTCTTCAA GGCCATGAGC CACATGCTGT 
     2461 GTATTGGGTA CGGACGGCAG GCACCCGTAG GCATGTCTGA CGTCTGGCTC ACCATGCTCA 
     2521 GCATGATCGT GGGCGCCACC TGCTATGCCA TGTTCATCGG GCACGCCACT GCCCTCATCC 
     2581 AGTCGCTGGA CTCCTCCCGG CGCCAGTACC AGGAGAAGTA CAAGCAGGTG GAGCAGTACA 
     2641 TGTCCTTCCA CAAGCTCCCG CCTGACACCA GGCAGCGCAT CCACGACTAC TACGAACACC 
     2701 GCTACCAGGG CAAGATGTTT GACGAGGAAA GCATCCTGGG TGAGCTGAGT GAGCCGCTTC 
     2761 GAGAGGAGAT CATCAACTTT AACTGCCGGA AGCTGGTGGC ATCCATGCCA CTGTTCGCCA 
     2821 ATGCAGACCC CAACTTTGTG ACGTCTATGC TGACCAAGTT GCGTTTTGAG GTCTTTCAGC 
     2881 CTGGGGACTA CATCATCCGT GAAGGCACCA TCGGCAAGAA GATGTACTTT ATCCAGCACG 
     2941 GCGTGGTCAG TGTGCTCACT AAGGGCAACA AGGAGACCAA GCTGGCTGAT GGCTCCTATT 
     3001 TTGGAGAGAT CTGCTTGCTG ACCCGAGGCC GTCGCACAGC GAGCGTGAGG GCGGATACTT 
     3061 ACTGCCGCCT CTACTCACTG AGCGTGGACA ACTTCAACGA GGTGCTGGAG GAGTATCCCA 
     3121 TGATGCGCAG GGCTTTCGAG ACGGTTGCGC TGGACCGTCT GGACCGCATA GGCAAGAAGA 
     3181 ACTCCATCCT CCTCCACAAG GTGCAGCACG ACCTCAACTC AGGCGTCTTC AACTACCAAG 
     3241 AGAACGAGAT CATCCAGCAG ATCGTGCGGC ATGACCGTGA GATGGCCCAC TGTGCTCACC 
     3301 GCGTCCAGGC TGCTGCCTCA GCCACCCCAA CCCCAACGCC TGTCATATGG ACCCCACTGA 
     3361 TCCAGGCACC ACTGCAGGCT GCTGCTGCTA CTACTTCGGT GGCCATAGCC CTCACACACC 
     3421 ACCCCCGCCT GCCAGCCGCT ATCTTCCGGC CCCCTCCCGG ACCTGGGCTG GGTAACCTGG 
     3481 GGGCTGGACA GACACCGAGG CACCCAAGGA GGTTGCAGTC CTTGATCCCT TCAGCGCTAG 
     3541 GCTCTGCTTC ACCAGCCAGC AGCCCCTCAC AGGTGGACAC ACCGTCTTCA TCTTCCTTCC 
     3601 ACATCCAACA GCTGGCTGGA TTCTCTGCAC CTCCTGGATT GAGTCCTCTC TTGCCCTCCT 
     3661 CTAGCTCTTC CCCACCTCCA GGAGCCTGCA GTTCTCCCCC AGCCCCCACT CCATCCACCT 
     3721 CCACTGCTGC CACCACCACC GGGTTCGGCC ACTTTCATAA GGCGCTAGGT GGCTCCCTGT 
     3781 CTTCCTCTGA TTCCCCGCTG CTCACCCCAC TGCAACCGGG CGCTCGCTCT CCACAGGCTG 
     3841 CCCAGCCGCC ACCCCCACTG CCTGGGGCCC GAGGAGGCCT GGGACTCCTG GAGCACTTCT 
     3901 TGCCGCCCCC ACCTTCGTCC CGGTCACCAT CATCTAGCCC TGGGCAGCTG GGCCAGCCTC 
     3961 CTGGAGAGTT GTCCCCAGGT CTGGCAGCTG GTCCACCAAG TACACCAGAG ACACCCCCGC 
     4021 GGCCCGAACG GCCATCCTTT ATGGCAGGGG CCTCTGGGGG GGCTTCTCCT GTAGCCTTTA 
     4081 CCCCCCGAGG AGGCCTCAGC CCTCCGGGCC ACAGCCCAGG ACCCCCAAGA ACTTTCCCGA 
     4141 GTGCCCCACC CCGGGCCTCT GGCTCCCATG GTTCCCTGCT CCTGCCACCT GCATCCAGCC 
     4201 CTCCGCCTCC CCAGGTCCCA CAGCGCAGGG GCACACCACC CCTCACCCCC GGCCGCCTCA 
     4261 CACAGGACCT GAAGCTCATC TCAGCCTCTC AGCCAGCCCT CCCCCAGGAT GGGGCACAGA 
     4321 CTCTACGCAG GGCCTCTCCT CACTCCTCAG GGGAGTCGAT GGCTGCCTTC TCACTCTACC 
     4381 CCAGAGCTGG GGGTGGCAGT GGGAGCAGTG GGGGCCTTGG GCCTCCTGGA AGGCCATATG 
     4441 GTGCCATCCC AGGCCAGCAT GTCACTTTGC CTCGGAAGAC ATCCTCAGGT TCTTTGCCAC 
     4501 CCCCACTTTC TTTGTTTGGG GCAAGAGCCG CCTCTTCTGG AGGGCCCCCT CTGACTGCTG 
     4561 CACCCCAGAG GGAACCTGGC GCTAGGTCCG AGCCAGTACG CTCCAAACTG CCGTCTAATT 
     4621 TAACGCGTAC GCGGCCGCTC GAGCAGAAAC TCATCTCAGA AGAGGATCTG GCAGCAAATG 
     4681 ATATCCTGGA TTACAAGGAT GACGACGATA AGGTTTAAAC GGCCGGCCGC GGTCATAGCT 
     4741 GTTTCCTGAA CAGATCCCGG GTGGCATCCC TGTGACCCCT CCCCAGTGCC TCTCCTGGCC 
     4801 CTGGAAGTTG CCACTCCAGT GCCCACCAGC CTTGTCCTAA TAAAATTAAG TTGCATCATT 
     4861 TTGTCTGACT AGGTGTCCTT CTATAATATT ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG 
     4921 GGGCAAGTTG GGAAGACAAC CTGTAGGGCC TGCGGGGTCT ATTGGGAACC AAGCTGGAGT 
     4981 GCAGTGGCAC AATCTTGGCT CACTGCAATC TCCGCCTCCT GGGTTCAAGC GATTCTCCTG 
     5041 CCTCAGCCTC CCGAGTTGTT GGGATTCCAG GCATGCATGA CCAGGCTCAG CTAATTTTTG 
     5101 TTTTTTTGGT AGAGACGGGG TTTCACCATA TTGGCCAGGC TGGTCTCCAA CTCCTAATCT 
     5161 CAGGTGATCT ACCCACCTTG GCCTCCCAAA TTGCTGGGAT TACAGGCGTG AACCACTGCT 
     5221 CCCTTCCCTG TCCTTCTGAT TTTAAAATAA CTATACCAGC AGGAGGACGT CCAGACACAG 
     5281 CATAGGCTAC CTGGCCATGC CCAACCGGTG GGACATTTGA GTTGCTTGCT TGGCACTGTC 
     5341 CTCTCATGCG TTGGGTCCAC TCAGTAGATG CCTGTTGAAT TGGGTACGCG GCCAGCGGCG 
     5401 AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG GGGATAACGC 
     5461 AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT 
     5521 GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG 
     5581 TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC 
     5641 CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC 
     5701 TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT 
     5761 CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT 
     5821 ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC 
     5881 AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA 
     5941 GTGGTGGCCT AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA 
     6001 GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG 
     6061 TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA 
     6121 AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG 
     6181 GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG 
     6241 AAGTTTTAAA TCAATCTAAA GTATATATGA GTAACCTGAG GCTATGGCAG GGCCTGCCGC 
     6301 CCCGACGTTG GCTGCGAGCC CTGGGCCTTC ACCCGAACTT GGGGGGTGGG GTGGGGAAAA 
     6361 GGAAGAAACG CGGGCGTATT GGCCCCAATG GGGTCTCGGT GGGGTATCGA CAGAGTGCCA 
     6421 GCCCTGGGAC CGAACCCCGC GTTTATGAAC AAACGACCCA ACACCGTGCG TTTTATTCTG 
     6481 TCTTTTTATT GCCGTCATAG CGCGGGTTCC TTCCGGTATT GTCTCCTTCC GTGTTTCAGT 
     6541 TAGCCTCCCC CTAGGGTGGG CGAAGAACTC CAGCATGAGA TCCCCGCGCT GGAGGATCAT 
     6601 CCAGCCGGCG TCCCGGAAAA CGATTCCGAA GCCCAACCTT TCATAGAAGG CGGCGGTGGA 
     6661 ATCGAAATCT CGTGATGGCA GGTTGGGCGT CGCTTGGTCG GTCATTTCGA ACCCCAGAGT 
     6721 CCCGCTCAGA AGAACTCGTC AAGAAGGCGA TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG 
     6781 GCGATACCGT AAAGCACGAG GAAGCGGTCA GCCCATTCGC CGCCAAGCTC TTCAGCAATA 
     6841 TCACGGGTAG CCAACGCTAT GTCCTGATAG CGATCCGCCA CACCCAGCCG GCCACAGTCG 
     6901 ATGAATCCAG AAAAGCGGCC ATTTTCCACC ATGATATTCG GCAAGCAGGC ATCGCCATGG 
     6961 GTCACGACGA GATCCTCGCC GTCGGGCATG CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT 
     7021 GGCGCGAGCC CCTGATGCTC TTCGTCCAGA TCATCCTGAT CGACAAGACC GGCTTCCATC 
     7081 CGAGTACGTG CTCGCTCGAT GCGATGTTTC GCTTGGTGGT CGAATGGGCA GGTAGCCGGA 
     7141 TCAAGCGTAT GCAGCCGCCG CATTGCATCA GCCATGATGG ATACTTTCTC GGCAGGAGCA 
     7201 AGGTGAGATG ACAGGAGATC CTGCCCCGGC ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC 
     7261 GCTTCAGTGA CAACGTCGAG CACAGCTGCG CAAGGAACGC CCGTCGTGGC CAGCCACGAT 
     7321 AGCCGCGCTG CCTCGTCTTG CAGTTCATTC AGGGCACCGG ACAGGTCGGT CTTGACAAAA 
     7381 AGAACCGGGC GCCCCTGCGC TGACAGCCGG AACACGGCGG CATCAGAGCA GCCGATTGTC 
     7441 TGTTGTGCCC AGTCATAGCC GAATAGCCTC TCCACCCAAG CGGCCGGAGA ACCTGCGTGC 
     7501 AATCCATCTT GTTCAATCAT GCGAAACGAT CCTCATCCTG TCTCTTGATC GATCTTTGCA 
     7561 AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA CTACTTCTGG AATAGCTCAG AGGCCGAGGC 
     7621 GGCCTCGGCC TCTGCATAAA TAAAAAAAAT TAGTCAGCCA TGGGGCGGAG AATGGGCGGA 
     7681 ACTGGGCGGA GTTAGGGGCG GGATGGGCGG AGTTAGGGGC GGGACTATGG TTGCTGACTA 
     7741 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCT 
     7801 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     7861 CTTTCCACAC CCTAACTGAC ACACATTCCA CAGCTGGTTC TTTCCGCCTC AGGACTCTTC 
     7921 CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT 
     7981 GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA 
     8041 CCTGACGCGC CCTGTAGCGG CGCATTAAGC GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG 
     8101 ACCGCTACAC TTGCCAGCGC CCTAGCGCCC GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC 
     8161 GCCACGTTCG CCGGCTTTCC CCGTCAAGCT CTAAATCGGG GGCTCCCTTT AGGGTTCCGA 
     8221 TTTAGTGCTT TACGGCACCT CGACCCCAAA AAACTTGATT AGGGTGATGG TTCACGTAGT 
     8281 GGGCCATCGC CCTGATAGAC GGTTTTTCGC CCTTTGACGT TGGAGTCCAC GTTCTTTAAT 
     8341 AGTGGACTCT TGTTCCAAAC TGGAACAACA CTCAACCCTA TCTCGGTCTA TTCTTTTGAT 
     8401 TTATAAGGGA TTTTGCCGAT TTCGGCCTAT TGGTTAAAAA ATGAGCTGAT TTAACAAAAA 
     8461 TTTAACGCGA ATTTT
//