LOCUS       RR214757                7626 bp    DNA     circular  SYN 
DEFINITION  Usp20 (Myc-DDK-tagged ORF) - Rat ubiquitin specific peptidase 20 (Usp20), (10 ug)
ACCESSION   NM_001107827
VERSION     NM_001107827.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3783..3874 
                     /label="Myc-DDK"  
     misc_feature    3904..4489 
                     /label="PolyA signal"  
     misc_feature    3961..3980 
                     /label="Restriction Enzyme"  
     misc_feature    4765..5437 
                     /label="ColE1"  
     misc_feature    5867..6661 
                     /label="Kan/Neo"  
     misc_feature    6696..6998 
                     /label="SV40 ori"  
     misc_feature    7084..7561 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3765..3771 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3765 
                     /label="Usp20"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGGGGACGCC AGAGACATCT GCCCTCACCT GGACTCCATA GGGGAGGTGA 
     1081 CCAAAGAGGA CTTGCTGCTT AAATCTAAGG GGACCTGCCA GTCCTGTGGT GTTGCTGGAC 
     1141 CTAACCTATG GGCTTGCCTC CAGGTCACCT GCCCCTATGT TGGCTGTGGA GAGTCTTTTG 
     1201 CTGACCATAG CAGCATTCAT GCACAGGTGA AAAGGCACAA CCTGACTGTG AACCTGACTA 
     1261 CGTTACGGGT GTGGTGCTAC GCCTGTGAGC GGGAAGTCTT TCTGGAGCAG CGCCTGGCAG 
     1321 TCCACCTGCC CAGCTCCTCA GCCAGGCTCT CAGAGCAGGA CTCCCCACCA CCCTGCCACC 
     1381 CTCTGAAAGC TGTCCCTATT GCCGTGGCTG ATGAGGGAGA GTCTGAGTCT GAGGACGATG 
     1441 ACCTGAAGCC TCGAGGCCTC ACAGGCATGA AGAACCTGGG GAACTCCTGT TACATGAATG 
     1501 CTGCCCTGCA GGCCTTGTCC AATTGCCCCC CACTCACCCA GTTCTTCCTG GAGTGTGGTG 
     1561 GCCTGGTGCG AACAGACAAG AAGCCAGCCT TGTGCAAAAG TTACCAGAAG TTGATCTCTG 
     1621 AGGTCTGGCA CAAGAAACGG CCAAGCTATG TGGTCCCCAC CAGCCTATCC CATGGGATCA 
     1681 AGTTGGTCAA CCCGATGTTC CGAGGCTATG CCCAGCAGGA CACCCAGGAG TTCCTACGCT 
     1741 GCCTGATGGA CCAGCTTCAC GAGGAGCTCA AGGAGCCCAT GGTAGCTGCT GTGGCAGCTC 
     1801 TCACTGATGC TCGGGACTCA GATTCCAGTG ACACAGATGA GAGGCGTGAT GGCGACCGGA 
     1861 GCCCTTCAGA GGACGAGTTT CTGTCCTGTG ACTCGAGCAG TGACAGGGGT GAGGGTGATG 
     1921 GGCAGGGCCG TGGTGGAGGC AGCTCAAAGG CTGAGATGGA GCTGCTGATC TCAGACGAGG 
     1981 CGGGCCGAGC CATCTCTGAG AAAGAGCGAA TGAAGGACCG CAAGTTCTCC TGGGGCCAGC 
     2041 AGCGCACCAA CTCGGAGCAA GTGGATGAGG ACGCTGATGT GGACACAGCC ATGGCCTCCC 
     2101 TTGACGAACA ATCTAGAGAG GCCCAGCCAC CGTCACCAAG GCCTACCAGC CCCTGCCAGA 
     2161 CCCCAGAGCC AGACAATGAA GTCCACATTC GCAGCTCCTC TCGCCCCTGC AGCCCTGTCC 
     2221 ACCACCACGA GGGCCATACC AAGCTGTCCA GCAGCCCTCC TCGTGCAAGC CCCGTGAGGA 
     2281 TGGGGCCATC GTACGTGCTC AAGAAAGCCC AGGTGCCGAG TACTGGTGGC CGGAGGCGGA 
     2341 AGGAACAGAG CTACCGCAGT GTCATCTCAG ATGTCTTCAA TGGCTCTGTC CTCAGCCTGG 
     2401 TGCAGTGTCT TACCTGTGAT CGGGTGTCTA CCACAGTGGA GACATTCCAG GACCTGTCAT 
     2461 TGCCCATCCC TGGCAAGGAA GACCTAGCCA AACTTCACTC TGCCATCTAC CAGAATGTAC 
     2521 CAGCCAAGCC GGGTGCCTGT GGGGACAGCT ATTCCTCCCA GGGCTGGCTG GCCTTCATCG 
     2581 TGGAGTACAT CCGACGGTTT GTGGTATCCT GTACCCCCAG CTGGTTTTGG GGACCTGTTG 
     2641 TCACACTGGA AGACTGCCTG GCTGCTTTCT TTGCTGCTGA TGAGCTGAAG GGTGACAATA 
     2701 TGTACAGCTG TGAGCGCTGT AAGAAGCTTC GGAATGGCGT GAAGTACTGT AAAGTCCTCT 
     2761 GTCTGCCTGA GATTCTGTGT GTCCACCTGA AGCGCTTCCG GCATGAGGTG ATGTACTCCT 
     2821 TCAAAGTCAG CAGCCATGTC TCTTTCCCCC TGGAGGGACT TGACCTACGC CCTTTTCTAG 
     2881 CCAAGGAGTG CACATCCCAG GTCACCACCT ATGACCTCCT CTCTGTCATT TGTCACCACG 
     2941 GCACAGCAGG CAGTGGGCAC TACATTGCCT ACTGTCAGAA CGTGATCAAT GGGCAGTGGT 
     3001 ATGAGTTTGA TGACCAGTAC GTCACCGAGG TCCATGAGAC TGTGGTACAG AATGTGGAGG 
     3061 CCTACGTGCT CTTCTACAGG AAGAGCAGTG AGGAGGCCAT GCGTGAGCGG CAGCAGGTGG 
     3121 TGTCCCTGGC TGCCATGCAG GAACCCAGCC TGCTCCGCTT CTACGTGTCC CGAGAATGGC 
     3181 TCAACAAGTT CAACACCTTT GCAGAGCCAG GGCCCATCAC CAACCACACC TTCCTGTGCC 
     3241 CCCATGGAGG TATCCCACCC AATAAATACC ATTACATTGA TGACCTGGTG GTCATTCTGC 
     3301 CGCAGAGCGT CTGGGAGCAC CTGTACAGCA GGTTTGGGGG CGGCCCTGCT GTGAACCATC 
     3361 TGTATGTGTG CTCCATCTGC CAGGTGGAGA TTGAGGCCCT GGCCAAGCGC AGGAGGGTGG 
     3421 AGATTGACAC TTTCATCAAG CTGAACAAGG CATTCCAAGC CGAGGAGTCT CCCGGGGTCA 
     3481 TCTACTGCAT CAGCATGCAC TGGTTCCGAG AGTGGGAGGC TTTCGTCAAG GGGAAGGACA 
     3541 GCGAGCCTCC AGGGCCCATC GACAACAGCA GGATTGCACA GGTCAAAGGC AGTGGTCATA 
     3601 TCCAGCTAAA GCAGGGGGCT GACTACGGGC AGATTTCTGA GGAGACCTGG ACCTATCTGA 
     3661 ACAGTCTGTA TGGGGGTGGC CCTGAGATTG CCATCCGGCA GAGTGTGGCC CAGCTCCCAG 
     3721 ACCCAGAAAG CTTGCATGGA GAGCAGAAGA TTGAGGCTGA AACCCGGGCT CTGACGCGTA 
     3781 CGCGGCCGCT CGAGCAGAAA CTCATCTCAG AAGAGGATCT GGCAGCAAAT GATATCCTGG 
     3841 ATTACAAGGA TGACGACGAT AAGGTTTAAA CGGCCGGCCG CGGTCATAGC TGTTTCCTGA 
     3901 ACAGATCCCG GGTGGCATCC CTGTGACCCC TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT 
     3961 GCCACTCCAG TGCCCACCAG CCTTGTCCTA ATAAAATTAA GTTGCATCAT TTTGTCTGAC 
     4021 TAGGTGTCCT TCTATAATAT TATGGGGTGG AGGGGGGTGG TATGGAGCAA GGGGCAAGTT 
     4081 GGGAAGACAA CCTGTAGGGC CTGCGGGGTC TATTGGGAAC CAAGCTGGAG TGCAGTGGCA 
     4141 CAATCTTGGC TCACTGCAAT CTCCGCCTCC TGGGTTCAAG CGATTCTCCT GCCTCAGCCT 
     4201 CCCGAGTTGT TGGGATTCCA GGCATGCATG ACCAGGCTCA GCTAATTTTT GTTTTTTTGG 
     4261 TAGAGACGGG GTTTCACCAT ATTGGCCAGG CTGGTCTCCA ACTCCTAATC TCAGGTGATC 
     4321 TACCCACCTT GGCCTCCCAA ATTGCTGGGA TTACAGGCGT GAACCACTGC TCCCTTCCCT 
     4381 GTCCTTCTGA TTTTAAAATA ACTATACCAG CAGGAGGACG TCCAGACACA GCATAGGCTA 
     4441 CCTGGCCATG CCCAACCGGT GGGACATTTG AGTTGCTTGC TTGGCACTGT CCTCTCATGC 
     4501 GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA TTGGGTACGC GGCCAGCGGC GAGCGGTATC 
     4561 AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA 
     4621 CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT 
     4681 TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG 
     4741 GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG 
     4801 CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG 
     4861 CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC 
     4921 CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA 
     4981 CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG 
     5041 TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC 
     5101 TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC 
     5161 CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG 
     5221 TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT 
     5281 GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT 
     5341 CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA 
     5401 ATCAATCTAA AGTATATATG AGTAACCTGA GGCTATGGCA GGGCCTGCCG CCCCGACGTT 
     5461 GGCTGCGAGC CCTGGGCCTT CACCCGAACT TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC 
     5521 GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG TGGGGTATCG ACAGAGTGCC AGCCCTGGGA 
     5581 CCGAACCCCG CGTTTATGAA CAAACGACCC AACACCGTGC GTTTTATTCT GTCTTTTTAT 
     5641 TGCCGTCATA GCGCGGGTTC CTTCCGGTAT TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC 
     5701 CCTAGGGTGG GCGAAGAACT CCAGCATGAG ATCCCCGCGC TGGAGGATCA TCCAGCCGGC 
     5761 GTCCCGGAAA ACGATTCCGA AGCCCAACCT TTCATAGAAG GCGGCGGTGG AATCGAAATC 
     5821 TCGTGATGGC AGGTTGGGCG TCGCTTGGTC GGTCATTTCG AACCCCAGAG TCCCGCTCAG 
     5881 AAGAACTCGT CAAGAAGGCG ATAGAAGGCG ATGCGCTGCG AATCGGGAGC GGCGATACCG 
     5941 TAAAGCACGA GGAAGCGGTC AGCCCATTCG CCGCCAAGCT CTTCAGCAAT ATCACGGGTA 
     6001 GCCAACGCTA TGTCCTGATA GCGATCCGCC ACACCCAGCC GGCCACAGTC GATGAATCCA 
     6061 GAAAAGCGGC CATTTTCCAC CATGATATTC GGCAAGCAGG CATCGCCATG GGTCACGACG 
     6121 AGATCCTCGC CGTCGGGCAT GCTCGCCTTG AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC 
     6181 CCCTGATGCT CTTCGTCCAG ATCATCCTGA TCGACAAGAC CGGCTTCCAT CCGAGTACGT 
     6241 GCTCGCTCGA TGCGATGTTT CGCTTGGTGG TCGAATGGGC AGGTAGCCGG ATCAAGCGTA 
     6301 TGCAGCCGCC GCATTGCATC AGCCATGATG GATACTTTCT CGGCAGGAGC AAGGTGAGAT 
     6361 GACAGGAGAT CCTGCCCCGG CACTTCGCCC AATAGCAGCC AGTCCCTTCC CGCTTCAGTG 
     6421 ACAACGTCGA GCACAGCTGC GCAAGGAACG CCCGTCGTGG CCAGCCACGA TAGCCGCGCT 
     6481 GCCTCGTCTT GCAGTTCATT CAGGGCACCG GACAGGTCGG TCTTGACAAA AAGAACCGGG 
     6541 CGCCCCTGCG CTGACAGCCG GAACACGGCG GCATCAGAGC AGCCGATTGT CTGTTGTGCC 
     6601 CAGTCATAGC CGAATAGCCT CTCCACCCAA GCGGCCGGAG AACCTGCGTG CAATCCATCT 
     6661 TGTTCAATCA TGCGAAACGA TCCTCATCCT GTCTCTTGAT CGATCTTTGC AAAAGCCTAG 
     6721 GCCTCCAAAA AAGCCTCCTC ACTACTTCTG GAATAGCTCA GAGGCCGAGG CGGCCTCGGC 
     6781 CTCTGCATAA ATAAAAAAAA TTAGTCAGCC ATGGGGCGGA GAATGGGCGG AACTGGGCGG 
     6841 AGTTAGGGGC GGGATGGGCG GAGTTAGGGG CGGGACTATG GTTGCTGACT AATTGAGATG 
     6901 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC TGGTTGCTGA 
     6961 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     7021 CCCTAACTGA CACACATTCC ACAGCTGGTT CTTTCCGCCT CAGGACTCTT CCTTTTTCAA 
     7081 TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT 
     7141 TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC ACCTGACGCG 
     7201 CCCTGTAGCG GCGCATTAAG CGCGGCGGGT GTGGTGGTTA CGCGCAGCGT GACCGCTACA 
     7261 CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC GCTTTCTTCC CTTCCTTTCT CGCCACGTTC 
     7321 GCCGGCTTTC CCCGTCAAGC TCTAAATCGG GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT 
     7381 TTACGGCACC TCGACCCCAA AAAACTTGAT TAGGGTGATG GTTCACGTAG TGGGCCATCG 
     7441 CCCTGATAGA CGGTTTTTCG CCCTTTGACG TTGGAGTCCA CGTTCTTTAA TAGTGGACTC 
     7501 TTGTTCCAAA CTGGAACAAC ACTCAACCCT ATCTCGGTCT ATTCTTTTGA TTTATAAGGG 
     7561 ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA AATGAGCTGA TTTAACAAAA ATTTAACGCG 
     7621 AATTTT
//