LOCUS       RR214731                8505 bp    DNA     circular  SYN 
DEFINITION  Trpm4 (Myc-DDK-tagged ORF) - Rat transient receptor potential cation channel, subfamily M, member 4 (Trpm4), (10 ug)
ACCESSION   NM_001136229
VERSION     NM_001136229.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4662..4753 
                     /label="Myc-DDK"  
     misc_feature    4783..5368 
                     /label="PolyA signal"  
     misc_feature    4840..4859 
                     /label="Restriction Enzyme"  
     misc_feature    5644..6316 
                     /label="ColE1"  
     misc_feature    6746..7540 
                     /label="Kan/Neo"  
     misc_feature    7575..7877 
                     /label="SV40 ori"  
     misc_feature    7963..8440 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4644..4650 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4644 
                     /label="Trpm4"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGTGGGGCAG GAGAAAGAGC AGAGTTGGAT CCCTAAGATC TTCAGGAAGA 
     1081 AGGTGTGCAC GACGTTCATA GTGGACCTCC ACGATGATGC GGGAGGGACC TTGTGCCAGT 
     1141 GTGGGCAACC TCGGGATGCC CACCCTTCTG TGGCTGTGGA GGATGCCTTT GGGGCAGCCG 
     1201 TAGTTACCGA GTGGAACAGT GATGAGCACA CCACGGAGAA GCCCACAGAT GCCTACGGGG 
     1261 ACCTGGACTT CACATACTCT GGCCGAAAGT CCAGCAACTT CCTCCGGCTG TCTGATCGCA 
     1321 CGGATCCGGC CACTGTGTAC AGTCTCGTCA CCCGCTCTTG GGGCTTTCGT GCTCCAAACT 
     1381 TGGTGGTGTC AGTGCTGGGG GGGTCGGAGG GCCCCGTGCT ACAGACCTGG CTGCAGGACC 
     1441 TTCTGCGTCG TGGGCTGGTG CGAGCAGCCC AGAGCACAGG GGCCTGGATC GTCACTGGGG 
     1501 GACTGCACAC AGGCATTGGT CGGCATGTGG GTGTGGCTGT GAGGGACCAC CAGACGGCCA 
     1561 GCACCGGGGG CAGCAAGGTG GTGGCCATGG GTGTGGCCCC CTGGGGAGTG GTCCGGAACA 
     1621 GAGACATGCT GATCAACCCC AAGGGCTCAT TCCCTGCAAG ATACCGGTGG CGTGGTGACC 
     1681 CTGAGGACGG AGTTGAGTTC CCCCTGGACT ATAACTATTC TGCTTTCTTC TTGGTGGATG 
     1741 ATGGTACCTA TGGCCGCATG GGTGGTGAGA ACCGCTTCCG CCTTCGGTTT GAGTCCTATG 
     1801 TGGCTCAGCA GAAGACCGGA GTGGGAGGGA CTGGAATTGA CATCCCTGTA CTCCTTCTTC 
     1861 TCATCGAAGG CGATGAGAAG ATGTTAAAGC GGATAGAGGA TGCCACCCAG GCTCAGCTCC 
     1921 CCTGCCTCTT GGTGGCTGGC TCTGGGGGTG CTGCAGACTG CCTGGTGGAG ACCCTGGAAG 
     1981 ATACTCTGGC CCCAGGGAGT GGGGGACTCA GGCGAGGTGA AGCCCGGGAT CGAATTAGAC 
     2041 GTTACTTCCC TAAAGGAGAC CCCGAGGTCC TGCAGGCCCA GGTAGAGAGG ATCATGACCC 
     2101 GAAAGGAGCT GCTGACAGTC TATTCATCAG AGGACGGCTC CGAGGAGTTT GAGACTATCG 
     2161 TTTTGAGGGC TCTTGTGAAA GCCTGTGGGA GCTCCGAGGC CTCGGCTTAC CTGGATGAAC 
     2221 TGCGTTTGGC TGTGGCTTGG AACCGCGTGG ACATCGCCCA AAGTGAACTT TTCCGTGGGG 
     2281 ACATCCAGTG GCGGTCCTTC CACCTGGAGG CCTCTCTCAT GGACGCTCTG CTGAATGACC 
     2341 GGCCTGAGTT TGTGCGCTTG CTCATCTCCC ATGGCCTTAG TCTGGGACAC TTCCTGACCC 
     2401 CCGTGCGCCT GGCCCAGCTG TACAGCGCAG TGTCCCCCAA CTCGCTGATC CGCAATCTTC 
     2461 TGGACCAGGC GTCCCACGCT AGTAGCAGCA AATCTCCACC TGCAAATGGA GCTGCAGAGC 
     2521 TCCGGCCTCC TAATGTGGGG CAAGTTCTGA GGACTCTGTT GGGAGAAACG TGTGCACCGC 
     2581 GGTACCCCGC CAGGAACACC CGACACTCCC TGCTGGGCCA GGACCACAGA GAGAATGACT 
     2641 CTCTGCTCAT GGACTGGGCC AACATGCAAC AGGATGCAAG CTTTGAACAA GCCCCCTGGA 
     2701 GTGACCTGCT TATCTGGGCT CTGTTGTTGA ACCGAGCCCA GATGGCCATT TATTTCTGGG 
     2761 AGAAGGGCTC CAACTCAGTG GCCTCTGCTC TCGGGGCTTG TCTCTTACTC CGAGTGATGG 
     2821 CTCGCCTTGA GTGGGAGGCT GAGGAAGCTG CACGGCGGAA GGACCTTGCT GCCAAGTTTG 
     2881 AAAGCATGAG CGTGGACCTC TTTGGAGAGT GTTACCACAA CAGTGAATAT CGAGCAGCCC 
     2941 GCCTTCTTCT GCGTCGCTGT CCCCTCTGGG GAGAGGCCAC CTGTCTCCAG CTTGCCATGC 
     3001 AGGCTGACGC CCGTGCGTTC TTTGCCCAGG ATGGAGTACA GTCTCTGCTG ACACAGAAGT 
     3061 GGTGGGGGGA GATGGACAGC ACGAACCCTA TCTGGGCCCT GCTTCTCACC TTCTTCTGCC 
     3121 CGCCTCTCAT CTACACCAAC CTCATCCTCT TCAGGAAGTC AGAGGAGGAG CCCACACAGA 
     3181 AGGATCTTGA CTTCGATATG GACAGCAGCA TGAATGGAGC AGGTCCTCTT GGGCCTGCGG 
     3241 AGCCCTCAGC AAAGGTGGCC CTTGAGAGGC GGCGGCGGCG GCGGCCAGGA CATACCCTCT 
     3301 GCTGTGGCGG GTGCTCCAAG CGCTGGTCGT ACTTTTGGGG CGCCCCAGTG ACCGCCTTCC 
     3361 TGGGTAACGT GGTCAGTTAC CTGCTGTTCC TGCTGCTGTT TGCACACGTG TTGCTGGTGG 
     3421 ATTTCCAGCC TACAAAGCCG GGCGTCTTCG AGCTGCTGCT GTACTTCTGG GCCTTCACGC 
     3481 TGCTATGCGA GGAGCTGCGC CAGGGCCTGG GCGGTGGCTG GGGTACCCTG GCCAACGGGG 
     3541 GACCTGGTCC TGGCAAAGCT CCCCTGCGCC ATCGTCTGCA CCTCTACCTC TTGGATACTT 
     3601 GGAACCAGTG CGACTTGCTG GCACTCACCT GCTTCCTGCT GGGCGTTGGC TGCAGGCTGA 
     3661 CTCCTGGCCT GTTTGACTTG GGACGCACAG TCCTCTGTCT TGACTTCATG ATCTTCACAC 
     3721 TGCGCCTGCT GCACATCTTC ACGGTGAACA AGCAGCTGGG GCCCAAGATT GTCATCGTGA 
     3781 GCAAGATGAT GAAGGATGTA TTCTTTTTCC TCTTCTTCCT CTGCGTGTGG CTTGTAGCTT 
     3841 ATGGGGTGGC CACAGAGGGG ATCCTGAGGC CCCAGGACCG CAGTCTACCG AGCATCCTGC 
     3901 GCAGGGTCTT CTACCGTCCG TATCTGCAGA TCTTCGGACA AATTCCCCAG GAGGAAATGG 
     3961 ATGTGGCCCT CATGAATCCC AGTAACTGCT CTGCCGAGCG GGGCTCCTGG GCTCACCCGG 
     4021 AGGGGCCGGT CGCAGGCTCC TGTGTGTCCC AGTATGCCAA CTGGCTGGTG GTGTTGCTCC 
     4081 TCATCGTCTT CTTGCTGGTG GCCAATATCC TGCTGCTCAA TCTGCTCATC GCCATGTTCA 
     4141 GCTACACCTT CAACAAAGTG CATGGCAACA GCGACCTCTA CTGGAAGGCG CAGCGCTACA 
     4201 GCCTCATCCG GGAATTCCAT TCGCGGCCTG CCCTGGCCCC ACCCCTCATC ATCATCTCTC 
     4261 ACCTGCGTCT CCTCTTCAAG TGGCTGCGCA GGTGTCATAG GACTAACTTG CCCGCCTCCC 
     4321 CAGTCTTCGA GCACTTCCGT GTCTGTCTCT CTAAGGAAGC AGAGAGGACG CTGCTGACTT 
     4381 GGGAGTCTGT GCACAAAGAG AACTTCCTAT TGGCACAAGC TCGTGACAAG CGAGACAGCG 
     4441 ACTCGGAGCG TCTGAAACGC ACGTCCCAGA AGGTGGACAC TGCACTGAAG CAGCTGGGAC 
     4501 AGATCAGAGA GTATGACCGG CGTCTGAGGG GGCTGGAGAG AGAGGTCCAA CACTGTTCTC 
     4561 GAGTCCTGAC TTGGATGGCT GAGGCCCTTA GCCACTCTGC CTTACTGCCT CCAGGGGGGC 
     4621 CACCCCCTCC AAGCCCCACT GGGTCCAAAG ACACGCGTAC GCGGCCGCTC GAGCAGAAAC 
     4681 TCATCTCAGA AGAGGATCTG GCAGCAAATG ATATCCTGGA TTACAAGGAT GACGACGATA 
     4741 AGGTTTAAAC GGCCGGCCGC GGTCATAGCT GTTTCCTGAA CAGATCCCGG GTGGCATCCC 
     4801 TGTGACCCCT CCCCAGTGCC TCTCCTGGCC CTGGAAGTTG CCACTCCAGT GCCCACCAGC 
     4861 CTTGTCCTAA TAAAATTAAG TTGCATCATT TTGTCTGACT AGGTGTCCTT CTATAATATT 
     4921 ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG GGGCAAGTTG GGAAGACAAC CTGTAGGGCC 
     4981 TGCGGGGTCT ATTGGGAACC AAGCTGGAGT GCAGTGGCAC AATCTTGGCT CACTGCAATC 
     5041 TCCGCCTCCT GGGTTCAAGC GATTCTCCTG CCTCAGCCTC CCGAGTTGTT GGGATTCCAG 
     5101 GCATGCATGA CCAGGCTCAG CTAATTTTTG TTTTTTTGGT AGAGACGGGG TTTCACCATA 
     5161 TTGGCCAGGC TGGTCTCCAA CTCCTAATCT CAGGTGATCT ACCCACCTTG GCCTCCCAAA 
     5221 TTGCTGGGAT TACAGGCGTG AACCACTGCT CCCTTCCCTG TCCTTCTGAT TTTAAAATAA 
     5281 CTATACCAGC AGGAGGACGT CCAGACACAG CATAGGCTAC CTGGCCATGC CCAACCGGTG 
     5341 GGACATTTGA GTTGCTTGCT TGGCACTGTC CTCTCATGCG TTGGGTCCAC TCAGTAGATG 
     5401 CCTGTTGAAT TGGGTACGCG GCCAGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT 
     5461 ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA 
     5521 AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC 
     5581 TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA 
     5641 AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC 
     5701 GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC 
     5761 ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA 
     5821 ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC 
     5881 GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG 
     5941 GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG 
     6001 AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG 
     6061 CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA 
     6121 GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA 
     6181 CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT 
     6241 CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA 
     6301 GTAACCTGAG GCTATGGCAG GGCCTGCCGC CCCGACGTTG GCTGCGAGCC CTGGGCCTTC 
     6361 ACCCGAACTT GGGGGGTGGG GTGGGGAAAA GGAAGAAACG CGGGCGTATT GGCCCCAATG 
     6421 GGGTCTCGGT GGGGTATCGA CAGAGTGCCA GCCCTGGGAC CGAACCCCGC GTTTATGAAC 
     6481 AAACGACCCA ACACCGTGCG TTTTATTCTG TCTTTTTATT GCCGTCATAG CGCGGGTTCC 
     6541 TTCCGGTATT GTCTCCTTCC GTGTTTCAGT TAGCCTCCCC CTAGGGTGGG CGAAGAACTC 
     6601 CAGCATGAGA TCCCCGCGCT GGAGGATCAT CCAGCCGGCG TCCCGGAAAA CGATTCCGAA 
     6661 GCCCAACCTT TCATAGAAGG CGGCGGTGGA ATCGAAATCT CGTGATGGCA GGTTGGGCGT 
     6721 CGCTTGGTCG GTCATTTCGA ACCCCAGAGT CCCGCTCAGA AGAACTCGTC AAGAAGGCGA 
     6781 TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG GCGATACCGT AAAGCACGAG GAAGCGGTCA 
     6841 GCCCATTCGC CGCCAAGCTC TTCAGCAATA TCACGGGTAG CCAACGCTAT GTCCTGATAG 
     6901 CGATCCGCCA CACCCAGCCG GCCACAGTCG ATGAATCCAG AAAAGCGGCC ATTTTCCACC 
     6961 ATGATATTCG GCAAGCAGGC ATCGCCATGG GTCACGACGA GATCCTCGCC GTCGGGCATG 
     7021 CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT GGCGCGAGCC CCTGATGCTC TTCGTCCAGA 
     7081 TCATCCTGAT CGACAAGACC GGCTTCCATC CGAGTACGTG CTCGCTCGAT GCGATGTTTC 
     7141 GCTTGGTGGT CGAATGGGCA GGTAGCCGGA TCAAGCGTAT GCAGCCGCCG CATTGCATCA 
     7201 GCCATGATGG ATACTTTCTC GGCAGGAGCA AGGTGAGATG ACAGGAGATC CTGCCCCGGC 
     7261 ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC GCTTCAGTGA CAACGTCGAG CACAGCTGCG 
     7321 CAAGGAACGC CCGTCGTGGC CAGCCACGAT AGCCGCGCTG CCTCGTCTTG CAGTTCATTC 
     7381 AGGGCACCGG ACAGGTCGGT CTTGACAAAA AGAACCGGGC GCCCCTGCGC TGACAGCCGG 
     7441 AACACGGCGG CATCAGAGCA GCCGATTGTC TGTTGTGCCC AGTCATAGCC GAATAGCCTC 
     7501 TCCACCCAAG CGGCCGGAGA ACCTGCGTGC AATCCATCTT GTTCAATCAT GCGAAACGAT 
     7561 CCTCATCCTG TCTCTTGATC GATCTTTGCA AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA 
     7621 CTACTTCTGG AATAGCTCAG AGGCCGAGGC GGCCTCGGCC TCTGCATAAA TAAAAAAAAT 
     7681 TAGTCAGCCA TGGGGCGGAG AATGGGCGGA ACTGGGCGGA GTTAGGGGCG GGATGGGCGG 
     7741 AGTTAGGGGC GGGACTATGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     7801 TGCTGGGGAG CCTGGGGACT TTCCACACCT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     7861 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CCTAACTGAC ACACATTCCA 
     7921 CAGCTGGTTC TTTCCGCCTC AGGACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG 
     7981 GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG 
     8041 GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGACGCGC CCTGTAGCGG CGCATTAAGC 
     8101 GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG ACCGCTACAC TTGCCAGCGC CCTAGCGCCC 
     8161 GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC GCCACGTTCG CCGGCTTTCC CCGTCAAGCT 
     8221 CTAAATCGGG GGCTCCCTTT AGGGTTCCGA TTTAGTGCTT TACGGCACCT CGACCCCAAA 
     8281 AAACTTGATT AGGGTGATGG TTCACGTAGT GGGCCATCGC CCTGATAGAC GGTTTTTCGC 
     8341 CCTTTGACGT TGGAGTCCAC GTTCTTTAAT AGTGGACTCT TGTTCCAAAC TGGAACAACA 
     8401 CTCAACCCTA TCTCGGTCTA TTCTTTTGAT TTATAAGGGA TTTTGCCGAT TTCGGCCTAT 
     8461 TGGTTAAAAA ATGAGCTGAT TTAACAAAAA TTTAACGCGA ATTTT
//