LOCUS       RR214718                7635 bp    DNA     circular  SYN 
DEFINITION  Hk1 (Myc-DDK-tagged ORF) - Rat hexokinase 1 (Hk1), nuclear gene encoding mitochondrial protein, (10 ug)
ACCESSION   NM_012734
VERSION     NM_012734.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3792..3883 
                     /label="Myc-DDK"  
     misc_feature    3913..4498 
                     /label="PolyA signal"  
     misc_feature    3970..3989 
                     /label="Restriction Enzyme"  
     misc_feature    4774..5446 
                     /label="ColE1"  
     misc_feature    5876..6670 
                     /label="Kan/Neo"  
     misc_feature    6705..7007 
                     /label="SV40 ori"  
     misc_feature    7093..7570 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3774..3780 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3774 
                     /label="Hk1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GATCGCCGCG CAACTACTGG CCTATTACTT CACCGAGCTG AAGGATGACC 
     1081 AAGTCAAAAA GATTGACAAG TATCTGTACG CCATGCGGCT CTCTGATGAG ATTCTGATAG 
     1141 ATATCCTGAC ACGATTCAAG AAAGAGATGA AGAATGGCCT CTCCCGGGAT TATAATCCAA 
     1201 CAGCCTCCGT CAAGATGCTG CCCACCTTCG TCCGGTCCAT TCCGGACGGC TCAGAAAAGG 
     1261 GGGATTTCAT TGCCCTGGAT CTCGGCGGGT CTTCCTTTCG AATCCTGCGG GTGCAGGTGA 
     1321 ACCACGAGAA GAACCAGAAC GTCAGCATGG AGTCTGAGAT CTACGACACC CCAGAGAACA 
     1381 TCGTGCATGG CAGTGGAACC CAGCTTTTCG ATCATGTCGC TGACTGCCTG GGAGACTTCA 
     1441 TGGAGAAAAA GAAGATCAAG GACAAGAAGT TACCCGTGGG ATTCACATTT TCCTTCCCCT 
     1501 GCCGACAATC CAAGATAGAT GAGGCTGTAC TGATCACGTG GACAAAGCGG TTCAAAGCCA 
     1561 GTGGCGTGGA AGGAGCGGAT GTGGTCAAGT TGCTGAATAA AGCCATTAAG AAGCGAGGGG 
     1621 ACTATGATGC TAACATTGTC GCCGTGGTGA ATGACACAGT AGGGACCATG ATGACCTGCG 
     1681 GTTATGATGA CCAACAGTGT GAAGTCGGCC TGATCATTGG CACAGGCACC AATGCTTGCT 
     1741 ACATGGAGGA ACTGCGACAC ATCGACCTGG TGGAAGGCGA CGAGGGGAGG ATGTGTATTA 
     1801 ACACGGAATG GGGAGCCTTT GGGGATGATG GGTCCCTGGA AGACATCCGA ACCGAGTTTG 
     1861 ACAGAGAGTT AGACCGTGGA TCTCTCAACC CTGGGAAGCA GCTGTTCGAG AAGATGGTGA 
     1921 GCGGCATGTA CATGGGGGAG CTGGTCCGGC TAATCCTGGT GAAGATGGCC AAGGAAGGCC 
     1981 TCTTATTCGA AGGGCGCATC ACTCCAGAGC TGCTCACGAG GGGAAAGTTC AACACTAGTG 
     2041 ACGTGTCCGC CATTGAAAAG GATAAGGAAG GCATTCAAAA TGCCAAGGAA ATCTTAACCC 
     2101 GCTTGGGAGT GGAGCCGTCT GATGTTGACT GTGTGTCGGT CCAGCACATC TGCACGATCG 
     2161 TCTCCTTCCG ATCAGCCAAC CTGGTGGCCG CCACGCTCGG TGCCATCTTG AACCGCCTGC 
     2221 GGGACAACAA GGGCACACCA CGCCTGCGGA CCACGGTTGG CGTGGACGGT TCTCTCTACA 
     2281 AGATGCACCC ACAGTACTCC CGGCGGTTCC ACAAGACCCT GAGGCGCCTG GTGCCTGACT 
     2341 CCGACGTCCG TTTCCTCCTC TCAGAGAGTG GCACGGGCAA GGGGGCCGCC ATGGTGACGG 
     2401 CAGTAGCCTA CCGCCTGGCT GAGCAGCACC GGCAGATTGA GGAAACCCTG GCCCACTTCC 
     2461 GCCTCAGCAA GCAGACGCTG ATGGAGGTGA AGAAGAGGCT ACGGACAGAG ATGGAAATGG 
     2521 GGCTGAGGAA GGAGACCAAC AGCAAAGCTA CTGTCAAAAT GCTGCCTTCT TTTGTCCGGA 
     2581 GCATCCCGGA TGGGACTGAA CACGGTGACT TCCTGGCCTT GGATCTTGGA GGAACGAATT 
     2641 TCCGGGTTCT GCTGGTAAAG ATCCGCAGTG GGAAAAAGAG AACAGTGGAA ATGCACAACA 
     2701 AGATCTACTC CATTCCCCTG GAAATCATGC AGGGCACCGG GGATGAGCTG TTTGACCACA 
     2761 TCGTCTCCTG CATCTCTGAC TTCCTGGACT ACATGGGGAT CAAAGGCCCC CGGATGCCTC 
     2821 TGGGCTTCAC CTTCTCATTT CCCTGCCATC AGACGAACCT GGACTGTGGA ATCTTGATCT 
     2881 CATGGACAAA GGGTTTCAAA GCCACTGACT GTGAGGGCCA TGATGTAGCC TCCTTACTGA 
     2941 GGGATGCGGT GAAGAGGAGA GAGGAATTTG ACTTGGATGT GGTGGCTGTG GTCAACGACA 
     3001 CCGTGGGCAC CATGATGACC TGTGCGTATG AAGAACCCAC TTGCGAAATT GGACTCATCG 
     3061 TGGGGACGGG CACCAATGCC TGCTACATGG AGGAGATGAA GAATGTGGAG ATGGTGGAGG 
     3121 GGAACCAGGG CCAGATGTGC ATCAACATGG AGTGGGGCGC CTTCGGTGAC AATGGGTGTC 
     3181 TGGATGACAT CAGAACAGAC TTTGACAAAG TGGTGGACGA ATATTCTCTA AACTCTGGGA 
     3241 AACAAAGGTT TGAGAAAATG ATCAGTGGGA TGTACCTGGG TGAGATCGTC CGTAACATCC 
     3301 TGATTGACTT CACCAAGAAA GGCTTCCTCT TCCGGGGACA GATCTCCGAA CCACTCAAGA 
     3361 CCCGAGGCAT CTTTGAGACC AAGTTTCTCT CTCAGATTGA GAGTGACCGG TTAGCGCTGC 
     3421 TCCAGGTGCG GGCCATCCTT CAGCAGCTGG GTTTGAACAG CACGTGTGAC GACAGTATCC 
     3481 TGGTCAAGAC CGTGTGTGGG GTGGTGTCCA AGAGGGCGGC TCAGCTGTGT GGTGCCGGCA 
     3541 TGGCCGCCGT GGTGGAAAAG ATCAGAGAGA ACAGAGGCCT AGACCATCTG AATGTAACTG 
     3601 TGGGAGTGGA TGGGACGCTC TACAAACTTC ATCCACACTT CTCCAGAATC ATGCACCAAA 
     3661 CTGTGAAGGA ACTGTCACCA AAGTGTACCG TGTCCTTCCT CCTGTCTGAA GACGGCAGCG 
     3721 GCAAGGGGGC CGCCCTTATC ACAGCTGTGG GCGTGCGGCT CAGAGGAGAC CCTTCGATCG 
     3781 CCACGCGTAC GCGGCCGCTC GAGCAGAAAC TCATCTCAGA AGAGGATCTG GCAGCAAATG 
     3841 ATATCCTGGA TTACAAGGAT GACGACGATA AGGTTTAAAC GGCCGGCCGC GGTCATAGCT 
     3901 GTTTCCTGAA CAGATCCCGG GTGGCATCCC TGTGACCCCT CCCCAGTGCC TCTCCTGGCC 
     3961 CTGGAAGTTG CCACTCCAGT GCCCACCAGC CTTGTCCTAA TAAAATTAAG TTGCATCATT 
     4021 TTGTCTGACT AGGTGTCCTT CTATAATATT ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG 
     4081 GGGCAAGTTG GGAAGACAAC CTGTAGGGCC TGCGGGGTCT ATTGGGAACC AAGCTGGAGT 
     4141 GCAGTGGCAC AATCTTGGCT CACTGCAATC TCCGCCTCCT GGGTTCAAGC GATTCTCCTG 
     4201 CCTCAGCCTC CCGAGTTGTT GGGATTCCAG GCATGCATGA CCAGGCTCAG CTAATTTTTG 
     4261 TTTTTTTGGT AGAGACGGGG TTTCACCATA TTGGCCAGGC TGGTCTCCAA CTCCTAATCT 
     4321 CAGGTGATCT ACCCACCTTG GCCTCCCAAA TTGCTGGGAT TACAGGCGTG AACCACTGCT 
     4381 CCCTTCCCTG TCCTTCTGAT TTTAAAATAA CTATACCAGC AGGAGGACGT CCAGACACAG 
     4441 CATAGGCTAC CTGGCCATGC CCAACCGGTG GGACATTTGA GTTGCTTGCT TGGCACTGTC 
     4501 CTCTCATGCG TTGGGTCCAC TCAGTAGATG CCTGTTGAAT TGGGTACGCG GCCAGCGGCG 
     4561 AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG GGGATAACGC 
     4621 AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT 
     4681 GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG 
     4741 TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC 
     4801 CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC 
     4861 TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT 
     4921 CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT 
     4981 ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC 
     5041 AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA 
     5101 GTGGTGGCCT AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA 
     5161 GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG 
     5221 TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA 
     5281 AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG 
     5341 GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG 
     5401 AAGTTTTAAA TCAATCTAAA GTATATATGA GTAACCTGAG GCTATGGCAG GGCCTGCCGC 
     5461 CCCGACGTTG GCTGCGAGCC CTGGGCCTTC ACCCGAACTT GGGGGGTGGG GTGGGGAAAA 
     5521 GGAAGAAACG CGGGCGTATT GGCCCCAATG GGGTCTCGGT GGGGTATCGA CAGAGTGCCA 
     5581 GCCCTGGGAC CGAACCCCGC GTTTATGAAC AAACGACCCA ACACCGTGCG TTTTATTCTG 
     5641 TCTTTTTATT GCCGTCATAG CGCGGGTTCC TTCCGGTATT GTCTCCTTCC GTGTTTCAGT 
     5701 TAGCCTCCCC CTAGGGTGGG CGAAGAACTC CAGCATGAGA TCCCCGCGCT GGAGGATCAT 
     5761 CCAGCCGGCG TCCCGGAAAA CGATTCCGAA GCCCAACCTT TCATAGAAGG CGGCGGTGGA 
     5821 ATCGAAATCT CGTGATGGCA GGTTGGGCGT CGCTTGGTCG GTCATTTCGA ACCCCAGAGT 
     5881 CCCGCTCAGA AGAACTCGTC AAGAAGGCGA TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG 
     5941 GCGATACCGT AAAGCACGAG GAAGCGGTCA GCCCATTCGC CGCCAAGCTC TTCAGCAATA 
     6001 TCACGGGTAG CCAACGCTAT GTCCTGATAG CGATCCGCCA CACCCAGCCG GCCACAGTCG 
     6061 ATGAATCCAG AAAAGCGGCC ATTTTCCACC ATGATATTCG GCAAGCAGGC ATCGCCATGG 
     6121 GTCACGACGA GATCCTCGCC GTCGGGCATG CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT 
     6181 GGCGCGAGCC CCTGATGCTC TTCGTCCAGA TCATCCTGAT CGACAAGACC GGCTTCCATC 
     6241 CGAGTACGTG CTCGCTCGAT GCGATGTTTC GCTTGGTGGT CGAATGGGCA GGTAGCCGGA 
     6301 TCAAGCGTAT GCAGCCGCCG CATTGCATCA GCCATGATGG ATACTTTCTC GGCAGGAGCA 
     6361 AGGTGAGATG ACAGGAGATC CTGCCCCGGC ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC 
     6421 GCTTCAGTGA CAACGTCGAG CACAGCTGCG CAAGGAACGC CCGTCGTGGC CAGCCACGAT 
     6481 AGCCGCGCTG CCTCGTCTTG CAGTTCATTC AGGGCACCGG ACAGGTCGGT CTTGACAAAA 
     6541 AGAACCGGGC GCCCCTGCGC TGACAGCCGG AACACGGCGG CATCAGAGCA GCCGATTGTC 
     6601 TGTTGTGCCC AGTCATAGCC GAATAGCCTC TCCACCCAAG CGGCCGGAGA ACCTGCGTGC 
     6661 AATCCATCTT GTTCAATCAT GCGAAACGAT CCTCATCCTG TCTCTTGATC GATCTTTGCA 
     6721 AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA CTACTTCTGG AATAGCTCAG AGGCCGAGGC 
     6781 GGCCTCGGCC TCTGCATAAA TAAAAAAAAT TAGTCAGCCA TGGGGCGGAG AATGGGCGGA 
     6841 ACTGGGCGGA GTTAGGGGCG GGATGGGCGG AGTTAGGGGC GGGACTATGG TTGCTGACTA 
     6901 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCT 
     6961 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     7021 CTTTCCACAC CCTAACTGAC ACACATTCCA CAGCTGGTTC TTTCCGCCTC AGGACTCTTC 
     7081 CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT 
     7141 GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA 
     7201 CCTGACGCGC CCTGTAGCGG CGCATTAAGC GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG 
     7261 ACCGCTACAC TTGCCAGCGC CCTAGCGCCC GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC 
     7321 GCCACGTTCG CCGGCTTTCC CCGTCAAGCT CTAAATCGGG GGCTCCCTTT AGGGTTCCGA 
     7381 TTTAGTGCTT TACGGCACCT CGACCCCAAA AAACTTGATT AGGGTGATGG TTCACGTAGT 
     7441 GGGCCATCGC CCTGATAGAC GGTTTTTCGC CCTTTGACGT TGGAGTCCAC GTTCTTTAAT 
     7501 AGTGGACTCT TGTTCCAAAC TGGAACAACA CTCAACCCTA TCTCGGTCTA TTCTTTTGAT 
     7561 TTATAAGGGA TTTTGCCGAT TTCGGCCTAT TGGTTAAAAA ATGAGCTGAT TTAACAAAAA 
     7621 TTTAACGCGA ATTTT
//