LOCUS       RR214716                8865 bp    DNA     circular  SYN 
DEFINITION  Abcc4 (Myc-DDK-tagged ORF) - Rat ATP-binding cassette, sub-family C (CFTR/MRP), member 4 (Abcc4), (10 ug)
ACCESSION   NM_133411
VERSION     NM_133411.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5020..5111 
                     /label="Myc-DDK"  
     misc_feature    5141..5726 
                     /label="PolyA signal"  
     misc_feature    5198..5217 
                     /label="Restriction Enzyme"  
     misc_feature    6002..6674 
                     /label="ColE1"  
     misc_feature    7104..7898 
                     /label="Kan/Neo"  
     misc_feature    7933..8235 
                     /label="SV40 ori"  
     misc_feature    8321..8798 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4995..5002 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4995 
                     /label="Abcc4"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCTGCCGGTG CACACCGAGG TGAAACCCAA CCCGCTGCAG GACGCCAACC 
     1081 TCTGCTCGCG CTTGTTCTTC TGGTGGCTCA ACCCGTTGTT TAAAGCTGGA CATAAGCGGA 
     1141 GATTGGAAGA AGATGACATG TTTTCAGTGC TTCCAGAAGA TCGCTCAAAG CACCTTGGAG 
     1201 AGGAGTTGCA AGGGTACTGG GATAAAGAAG TTCTGCGAGC CAAGAAGGAC GCTCGGAAGC 
     1261 CTTCCTTAAC GAAGGCAATC GTGAAGTGTT ACTGGAAATC TTACCTGATT TTGGGAATTT 
     1321 TTACGTTAAT TGAGGAGACC ACCCGAGTAG TTCAGCCCAT ATTTTTAGGG AAAATTATTG 
     1381 ATTATTTTGA GAAGTATGAC TCTGACGACT CGGCCGCTTT GCACACAGCT TACGGCTACG 
     1441 CGGCGGTGCT GTCGCTGTGC ACGCTCATCC TGGCCATACT ACATCACTTG TATTTCTACC 
     1501 ACGTGCAGTG CGCCGGGATG AGGATACGGG TCGCCATGTG CCACATGATT TACCGGAAGG 
     1561 CTCTCCGGCT AAGTAACTCG GCCATGGGGA AGACAACCAC GGGCCAGATA GTCAACCTGC 
     1621 TGTCCAACGA CGTGAACAAG TTTGACCAGG TGACCATCTT CTTGCACTTC CTGTGGGCGG 
     1681 GGCCGCTGCA AGCCATAGGG GTAACCATCC TTCTCTGGGT GGAAATCGGA ATCTCCTGTC 
     1741 TGGCGGGCCT GGCCATTCTG GTTATTCTGC TGCCTCTGCA AAGCTGCATC GGGAAACTGT 
     1801 TCTCGTCACT GCGGAGTAAA ACAGCGGCTT TCACGGATGC CAGGTTCCGG ACCATGAATG 
     1861 AAGTGATAAC GGGCATGAGG ATAATAAAGA TGTACGCGTG GGAGAAGTCA TTCGCCGACC 
     1921 TCATAACCAA TCTGAGAAAG AAGGAAATTT CTAAGATTCT GGGCAGCTCC TACCTCAGAG 
     1981 GGATGAACAT GGCGTCGTTT TTCATTGCAA ACAAAGTCAT CCTGTTCGTG ACCTTCACCA 
     2041 CCTATGTGCT GCTTGGCAAT AAGATTACAT CTAGCCACGT GTTCGTGGCC ATGACTCTGT 
     2101 ATGGTGCTGT CCGGTTGACA GTTACCCTCT TCTTCCCATC AGCCATTGAG AGAGTGTCAG 
     2161 AGGCCGTCGT CAGCGTTCGG AGGATCAAGA ACTTTCTGTT ACTCGATGAA CTGCCAGAGC 
     2221 GCAAAGCCCA GGAACCATCT GATGGCAAAG CCATCGTTCA CGTGCAAGAT TTTACCGCTT 
     2281 TCTGGGACAA GGCATTAGAC ACTCCTACCC TGCAAGGTCT TTCCTTCACT GCCAGACCTG 
     2341 GTGAGTTGTT AGCTGTGGTC GGCCCAGTCG GAGCAGGCAA GTCGTCCCTG TTGAGCGCGG 
     2401 TGCTCGGTGA GCTGCCTCCC ACCAGTGGGC TGGTCAGCGT GCACGGGAGG ATCGCCTACG 
     2461 TTTCCCAGCA GCCCTGGGTG TTCTCAGGAA CCGTGAGGAG CAATATTTTA TTTGGGAGGA 
     2521 AATACGAGAA AGAACGCTAC GAGAAAGTCA TCAAGGCCTG TGCTCTGAAG AAGGACTTGC 
     2581 AACTTCTGGA GGACGGGGAT CTGACTGTCA TAGGCGACCG GGGAGCCACG CTGAGCGGAG 
     2641 GCCAGAAAGC ACGGGTGAAC TTGGCACGGG CAGTCTACCA GGATGCCGAC ATCTACCTCC 
     2701 TCGATGATCC GCTCAGTGCT GTTGATGCAG AAGTGGGCAA GCACCTGTTC CAGCTGTGTA 
     2761 TCTGCCAGAC TTTGCACGAG AAGATCACCA TTTTAGTGAC TCACCAGTTA CAGTACCTCA 
     2821 AAGCTGCAAG CCACATCCTC ATACTAAAAG ATGGCGAAAT GGTGCAGAAG GGGACTTACA 
     2881 CCGAGTTTCT GAAGTCTGGT GTAGATTTCG GTTCCCTGCT AAAGAAGGAA AATGAGGAAG 
     2941 CAGAGCCCTC CCCTGTCCCA GGAACACCGA CACTCAGGAA CCGAACCTTC TCCGAGGCCT 
     3001 CGATTTGGTC TCAACAGTCA TCCAGACCCT CGTTGAAAGA CGGGGTCCCA GATGCCCAAG 
     3061 ATGCAGAGAA TACTCAGGCA GCTCAACCCG AGGAGAGCCG TTCAGAAGGG AGAATCGGCT 
     3121 TCAAGGCCTA CAAGAACTAC TTCTCTGCAG GCGCATCCTG GTTTTTCATT ATTTTCCTCG 
     3181 TTCTGCTTAA CCTGATGGGT CAGGTTTTCT ACGTTCTTCA GGACTGGTGG CTTTCCCACT 
     3241 GGGCGAATAG GCAAGGTGCC CTGAATGACA CCAAAAACGC GAACGGGAAT GTAACCGGGA 
     3301 CCCTCGATCT CAGCTGGTAC TTAGGGATTT ACACAGGTTT AACTGCGGTC ACAGTCCTCT 
     3361 TTGGCATAGC GAGATCCCTG CTGGTGTTCT ATGTCCTTGT GAATGCTTCC CAGACTCTGC 
     3421 ACAACAGGAT GTTCGAGTCG ATCCTGAAGG CACCGGTGTT GTTCTTCGAT AGAAATCCAA 
     3481 TAGGAAGGAT TTTAAATCGT TTCTCCAAGG ACATCGGACA CATGGACGAC TTGCTTCCCC 
     3541 TAACGTTTCT AGACTTCATC CAGACCTTGC TCCTTGTTGT AAGTGTGATC GCCGTGGCGG 
     3601 CGGCCGTGAT CCCTTGGATC CTCATACCCC TGGTTCCACT CTCCATCATC TTTGTGGTCC 
     3661 TTCGGAGATA TTTCTTAGAA ACGTCGCGGG ACGTCAAGCG CCTGGAGTCC ACAACACGGA 
     3721 GCCCGGTATT CTCCCATTTA TCATCCTCCC TCCAGGGACT CTGGACCATC CGTGCCTATA 
     3781 AGGCTGAAGA GAGGTGTCAG GAGCTGTTTG ATGCACACCA GGACTTGCAT TCAGAGGCTT 
     3841 GGTTCTTGTT CCTGACGACA TCAAGATGGT TCGCCGTGCG TCTGGACGCC ATCTGCGCCG 
     3901 TCTTTGTCAT CGTCGTTGCC TTCGGGTCCC TGGTTCTGGC AAAGACCTTG GATGCTGGGC 
     3961 AGGTTGGCTT GGCCTTGTCC TACTCCCTCA CGCTCATGGG GATGTTCCAG TGGTCTGTGA 
     4021 GACAGAGCGC CGAAGTAGAG AATATGATGA TTTCTGTGGA GAGGGTGATT GAATACACGG 
     4081 ACCTAGAGAA GGAGGCGCCT TGGGAATGCA GGAAGCGCCC ACCCCCAGGC TGGCCCCACG 
     4141 AAGGAGTAAT TGTCTTCGAC AATGTGAACT TCACCTACAG CTTGGACGGG CCCCTTGTTC 
     4201 TGAAGCACCT GACCGCACTC ATCAAGTCAC GAGAAAAGGT TGGAATTGTG GGAAGAACTG 
     4261 GAGCTGGAAA AAGTTCCCTC ATCTCAGCCC TCTTCAGGTT GTCAGAACCT GAGGGGAAAA 
     4321 TCTGGATTGA TAAGATCTTG ACGACTGAAA TTGGACTTCA CGATTTAAGG AAGAAAATGT 
     4381 CAATCATACC TCAGGAACCT GTCCTGTTCA CTGGAACCAT GAGGAAGAAC CTGGACCCCT 
     4441 TTAATGAGCA CTCGGACGAG GAGCTGTGGA AAGCCCTAGA GGAGGTACAA CTTAAAGAGG 
     4501 CCATAGAAGA TCTTCCTGGT AAAATGGACA CTGAACTAGC AGAATCTGGA TCCAATTTCA 
     4561 GTGTTGGACA GAGACAGTTA GTGTGCCTTG CGAGGGCAAT TCTGAAGAAA AACCGGATAC 
     4621 TGATCATTGA TGAAGCAACT GCAAATGTGG ATCCGAGAAC TGACGAGTTA ATACAACAGA 
     4681 AAATCCGGGA GAAGTTTGCC CAGTGCACTG TTCTCACCAT TGCTCACAGA CTGAACACCA 
     4741 TCATTGACAG CGACAAGATA ATGGTTTTGG ATTCGGGAAG ACTGAGAGAA TATGATGAGC 
     4801 CGTATGTTTT GCTGCAGAAT CCAGAGAGCC TCTTTTACAA GATGGTTCAG CAGCTGGGTA 
     4861 AGGGCGAAGC CGCTGCCCTC ACCGAAACAG CAAAACAGGT GTACTTCAGA CGGAATTATC 
     4921 CGGATATTGC ATTCAGCAGC CCTGCGGTTA TGAGCACCTC CAACGGACAG CCCTCCGCCT 
     4981 TAACGATATT TGAAACAGCA TTGAGCGGAC CGACGCGTAC GCGGCCGCTC GAGCAGAAAC 
     5041 TCATCTCAGA AGAGGATCTG GCAGCAAATG ATATCCTGGA TTACAAGGAT GACGACGATA 
     5101 AGGTTTAAAC GGCCGGCCGC GGTCATAGCT GTTTCCTGAA CAGATCCCGG GTGGCATCCC 
     5161 TGTGACCCCT CCCCAGTGCC TCTCCTGGCC CTGGAAGTTG CCACTCCAGT GCCCACCAGC 
     5221 CTTGTCCTAA TAAAATTAAG TTGCATCATT TTGTCTGACT AGGTGTCCTT CTATAATATT 
     5281 ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG GGGCAAGTTG GGAAGACAAC CTGTAGGGCC 
     5341 TGCGGGGTCT ATTGGGAACC AAGCTGGAGT GCAGTGGCAC AATCTTGGCT CACTGCAATC 
     5401 TCCGCCTCCT GGGTTCAAGC GATTCTCCTG CCTCAGCCTC CCGAGTTGTT GGGATTCCAG 
     5461 GCATGCATGA CCAGGCTCAG CTAATTTTTG TTTTTTTGGT AGAGACGGGG TTTCACCATA 
     5521 TTGGCCAGGC TGGTCTCCAA CTCCTAATCT CAGGTGATCT ACCCACCTTG GCCTCCCAAA 
     5581 TTGCTGGGAT TACAGGCGTG AACCACTGCT CCCTTCCCTG TCCTTCTGAT TTTAAAATAA 
     5641 CTATACCAGC AGGAGGACGT CCAGACACAG CATAGGCTAC CTGGCCATGC CCAACCGGTG 
     5701 GGACATTTGA GTTGCTTGCT TGGCACTGTC CTCTCATGCG TTGGGTCCAC TCAGTAGATG 
     5761 CCTGTTGAAT TGGGTACGCG GCCAGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT 
     5821 ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA 
     5881 AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC 
     5941 TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA 
     6001 AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC 
     6061 GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC 
     6121 ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA 
     6181 ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC 
     6241 GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG 
     6301 GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG 
     6361 AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG 
     6421 CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA 
     6481 GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA 
     6541 CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT 
     6601 CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA 
     6661 GTAACCTGAG GCTATGGCAG GGCCTGCCGC CCCGACGTTG GCTGCGAGCC CTGGGCCTTC 
     6721 ACCCGAACTT GGGGGGTGGG GTGGGGAAAA GGAAGAAACG CGGGCGTATT GGCCCCAATG 
     6781 GGGTCTCGGT GGGGTATCGA CAGAGTGCCA GCCCTGGGAC CGAACCCCGC GTTTATGAAC 
     6841 AAACGACCCA ACACCGTGCG TTTTATTCTG TCTTTTTATT GCCGTCATAG CGCGGGTTCC 
     6901 TTCCGGTATT GTCTCCTTCC GTGTTTCAGT TAGCCTCCCC CTAGGGTGGG CGAAGAACTC 
     6961 CAGCATGAGA TCCCCGCGCT GGAGGATCAT CCAGCCGGCG TCCCGGAAAA CGATTCCGAA 
     7021 GCCCAACCTT TCATAGAAGG CGGCGGTGGA ATCGAAATCT CGTGATGGCA GGTTGGGCGT 
     7081 CGCTTGGTCG GTCATTTCGA ACCCCAGAGT CCCGCTCAGA AGAACTCGTC AAGAAGGCGA 
     7141 TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG GCGATACCGT AAAGCACGAG GAAGCGGTCA 
     7201 GCCCATTCGC CGCCAAGCTC TTCAGCAATA TCACGGGTAG CCAACGCTAT GTCCTGATAG 
     7261 CGATCCGCCA CACCCAGCCG GCCACAGTCG ATGAATCCAG AAAAGCGGCC ATTTTCCACC 
     7321 ATGATATTCG GCAAGCAGGC ATCGCCATGG GTCACGACGA GATCCTCGCC GTCGGGCATG 
     7381 CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT GGCGCGAGCC CCTGATGCTC TTCGTCCAGA 
     7441 TCATCCTGAT CGACAAGACC GGCTTCCATC CGAGTACGTG CTCGCTCGAT GCGATGTTTC 
     7501 GCTTGGTGGT CGAATGGGCA GGTAGCCGGA TCAAGCGTAT GCAGCCGCCG CATTGCATCA 
     7561 GCCATGATGG ATACTTTCTC GGCAGGAGCA AGGTGAGATG ACAGGAGATC CTGCCCCGGC 
     7621 ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC GCTTCAGTGA CAACGTCGAG CACAGCTGCG 
     7681 CAAGGAACGC CCGTCGTGGC CAGCCACGAT AGCCGCGCTG CCTCGTCTTG CAGTTCATTC 
     7741 AGGGCACCGG ACAGGTCGGT CTTGACAAAA AGAACCGGGC GCCCCTGCGC TGACAGCCGG 
     7801 AACACGGCGG CATCAGAGCA GCCGATTGTC TGTTGTGCCC AGTCATAGCC GAATAGCCTC 
     7861 TCCACCCAAG CGGCCGGAGA ACCTGCGTGC AATCCATCTT GTTCAATCAT GCGAAACGAT 
     7921 CCTCATCCTG TCTCTTGATC GATCTTTGCA AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA 
     7981 CTACTTCTGG AATAGCTCAG AGGCCGAGGC GGCCTCGGCC TCTGCATAAA TAAAAAAAAT 
     8041 TAGTCAGCCA TGGGGCGGAG AATGGGCGGA ACTGGGCGGA GTTAGGGGCG GGATGGGCGG 
     8101 AGTTAGGGGC GGGACTATGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     8161 TGCTGGGGAG CCTGGGGACT TTCCACACCT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     8221 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CCTAACTGAC ACACATTCCA 
     8281 CAGCTGGTTC TTTCCGCCTC AGGACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG 
     8341 GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG 
     8401 GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGACGCGC CCTGTAGCGG CGCATTAAGC 
     8461 GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG ACCGCTACAC TTGCCAGCGC CCTAGCGCCC 
     8521 GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC GCCACGTTCG CCGGCTTTCC CCGTCAAGCT 
     8581 CTAAATCGGG GGCTCCCTTT AGGGTTCCGA TTTAGTGCTT TACGGCACCT CGACCCCAAA 
     8641 AAACTTGATT AGGGTGATGG TTCACGTAGT GGGCCATCGC CCTGATAGAC GGTTTTTCGC 
     8701 CCTTTGACGT TGGAGTCCAC GTTCTTTAAT AGTGGACTCT TGTTCCAAAC TGGAACAACA 
     8761 CTCAACCCTA TCTCGGTCTA TTCTTTTGAT TTATAAGGGA TTTTGCCGAT TTCGGCCTAT 
     8821 TGGTTAAAAA ATGAGCTGAT TTAACAAAAA TTTAACGCGA ATTTT
//