LOCUS       RR214641                9102 bp    DNA     circular  SYN 
DEFINITION  Edc4 (Myc-DDK-tagged ORF) - Rat enhancer of mRNA decapping 4 (Edc4), (10 ug)
ACCESSION   NM_001033068
VERSION     NM_001033068.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5259..5350 
                     /label="Myc-DDK"  
     misc_feature    5380..5965 
                     /label="PolyA signal"  
     misc_feature    5437..5456 
                     /label="Restriction Enzyme"  
     misc_feature    6241..6913 
                     /label="ColE1"  
     misc_feature    7343..8137 
                     /label="Kan/Neo"  
     misc_feature    8172..8474 
                     /label="SV40 ori"  
     misc_feature    8560..9037 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5241..5247 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5241 
                     /label="Edc4"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCCTCCTGC GCGAGCATCG ACATTGAGGA CGCCACGCAG CATCTGCGGG 
     1081 ACATCCTCAA ACTGGACCGG CCCGCCGGAG GCTCCAATGT CGAAAGCCAA AGGCCATCCA 
     1141 GTGCCTACAA TGGAGACCTC AATGGGCTCC TGGTCCCAGA TCCGCTCAGT TCAGGAGATG 
     1201 GTAACTCGAC AAGCAAACCT GGTATACGGA CCATGCCACC CATTAATCTG CAGGAAAAGC 
     1261 AGGTCATTTG CCTCTCTGGA GATGACAGCT CCACGTGCAT TGGGATTTTG GCCAAGGAGG 
     1321 TAGAAATTGT GGCCAGCAGT GATTCTAGCA TTTCCAGCAA GGCGCGAGGG AGCAACAAGG 
     1381 TGAAAATCCA GCCTGTAGCC AAGTATGACT GGGAGCAGAA ATACTACTAT GGCAACCTGA 
     1441 TTGCAGTGTC TAACTCCTTC TTGGCATACG CCATCAGGGC TGCCAACAAT GGCTCAGCCA 
     1501 TGGTCCGAGT GATCAGTGTG AGCACTTCGG AGCGGACCCT GCTCAAGGGC TTCACAGGCA 
     1561 GTGTGGCTGA CCTTGCATTT GCACACCTCA ACTCTCCACA GCTTGCCTGC CTGGACGAGG 
     1621 CCGGCGATCT CTTTGTGTGG CGACTGGCTC TGGTTAAGGG CAAAATTCAA GAAGAGATCT 
     1681 TAGTCCATAT CCGGCAACCA GAGGGCACAC CACTGAACCA CTTCCGAAGG ATCATCTGGT 
     1741 GCCCTTTCAT CCCTGAGGAA AGCGAGGACT GTTGTGAGGA GAGTAGCCCA ACGGTGGCCC 
     1801 TGCTGCATGA AGACCGGGCT GAGGTGTGGG ACTTGGATAT GCTTCGCTCC AGCCACAGCA 
     1861 CATGGCCTGT GGATGTCAGC CAAATCAAGC AGGGCTTTAT CGTGGTAAAA GGCCATAGCA 
     1921 CGTGCCTAAG TGAAGGAGCC CTTTCCCCTG ATGGGACTGT GCTGGCCACC GCAAGCCATG 
     1981 ATGGCTTTGT CAAGTTCTGG CAGATCTATA TTGAAGGTCA GGATGAGCCA AGGTGTCTGC 
     2041 ATGAATGGAA ACCTCATGAT GGGCGGCCTC TTTCTTGCCT CCTGTTCTGT GATAACCATA 
     2101 AGAAACAGGA TCCTGAGGTC CCATTCTGGA GGTTCCTCAT TACTGGCGCT GACCAGAATC 
     2161 GAGAGCTGAA GATGTGGTGC ACAGTGTCCT GGACCTGCCT GCAAACCATT CGCTTCTCCC 
     2221 CAGATATCTT CAGTTCAGTG AGTGTGCCTC CCAGTCTCAA AGTTTGCTTG GACCTCTCAG 
     2281 CAGAATATTT GATTCTTAGT GATGTGCAAC GAAAGGTCCT CTATGTGATG GAGCTGCTGC 
     2341 AGAACCAGGA CGAGGGCCGT GCTTGCTTCA GTTCCATCTC TGAGTTCCTG CTCACCCACC 
     2401 CTGTGCTGAG CTTTGGCATC CAGGTTGTGA GTCGCTGCCG GCTGCGGCAC ACTGAGGTGC 
     2461 TGCCTGCTGA GGAGGAGAGT GACAGTCTGG GGACTGAGAG TTCCCATGGA GCTGGTACCT 
     2521 TGGAATCTGC AGCTGGTGTG CTTATCAAGC TCTTTTGTGT GCACACTAAG GCACTCCAAG 
     2581 ATGTGCAGAT CCGCTTCCAG CCACAGCTGA ACCCTGATGT GGTGGCCCCA CTCTCTACCC 
     2641 ACACTGCTCA TGAGGACTTC ACATTTGGAG AGTCTCGACC TGAACTAGGC TCTGAGGGGC 
     2701 TAGCTTCAGC TCCTCATGGT TCCCAGCCTG ACCTCCGACG CATTGTGGAG CTGCCTGCAC 
     2761 CTGCAGACTT CCTCAGTCTG AGCAGTGAGA CCAAGCCTAA GTTGATGACA CCTGATGCTT 
     2821 TCATGACACC TACCGCCTCC CTGCAGCAGA TCTCTGCATC CCCTAGTAGT AGCAGCAGCA 
     2881 GTAGCAGCAG CAGCAGTAGC AGCAGCAGCA GTAGCAGCAG CAGCTCTCTA ACAGCTGTGT 
     2941 CTGCTGTGAG CAGCTCCTCA GCCATGGACC CCTCCTTGCC CAGTAGGCCA CCCGAGGAAC 
     3001 TGACCTTGAG CCCCAAACTG CAGCTAGATG GCAGCCTGAC TATAAGCAGC AGCAGCAGCC 
     3061 TGCAGGCAAG CCCTCGGAGC CTTCTTCCGG GCCTGCTCCC AGGCCCAGCT GACAAACTAA 
     3121 TTCCCAAGGG ACCTGGTCAG GTATCTTCTG GTACTTCTGC ACTATCCTTG GATTTGCAGG 
     3181 AAGTGGAACC ATTAGGGCTA CCCCAGGCCT CCCCAAGTCG CACACGTTCT CCTGATGTGA 
     3241 TCTCCTCAGC ATCCACTGCC CTGTCCCAGG ACATCCCTGA AATTGCATCT GAGGCCCTGT 
     3301 CCCGTGGCTT TGGCTCCTCT GTACCTGAGG GCCTCATTGA GCCAGACAGC ATGGCCTCAG 
     3361 CTGCCTCAGC ACTACACCTA CTGTCTCCTC GGCCCAGGCA AGGCCCTGAG CTTAGTTCTC 
     3421 AGCTTGGTCT AGATGGAGGC CCTGGGGATG GGGATAGGCA TAGTACCCCT TCCCTACTGG 
     3481 AAGCAGCCTT GACCCAGGAA GTTGCAACCT CTGACAGTCA AGTCTGGCCT ACAGCACCTG 
     3541 ACATTACTCG TGAGACCTGT AGCACACTAA CAGAAAGCCC CAGGAATGGC CTCCAGGAAA 
     3601 AGCACAAAAG CCTGGCCTTC CACAGGCCAC CTTATCACCT GCTGCAGCAA CATGACAGTC 
     3661 AGGACACAAG TGCTGAGCAA AGTGACCATG ACGATGAGGT TGCCAGCCTT GCCTCTGCTT 
     3721 CAGGAGGTTT TGGCAGCAAA ATTCCTACTC CACGGCTGCC TGCCAAGGAT TGGAAAACCA 
     3781 AGGGATCCCC TAGGACTTCA CCTAAGCTCA AGAGGAAAAG CAAGAAGGAT GATGGGGATT 
     3841 CAGCTGTGGG ATCTCGGCTC ACCGAGCACC AGGTGGTAGA GCCCCCTGAA GACTGGCCAG 
     3901 CACTAATTTG GCAGCAGCAA AGAGAGCTGG CAGAACTATG GCACAACCAA GAAGAGCTGC 
     3961 TACAGCGTCT TTGTGCCCAA CTTGAAGGTC TTCAGAGCAC TGTCACAGAC CATGTAGAAC 
     4021 GTGCCCTGGA GACACGGCAT GAGCAAGAGC AACGGCGACT GGAGCGGGCA CTGGCTGAGG 
     4081 GGCAGCAACG GGGTGGGCAG CTACAGGAGC AGCTGACACA GCAGCTATCC CAGGCTTTGT 
     4141 CTTCAGCTGT GGCTGGGCGG CTAGAGCGCA GCATAAGGGA TGAAATCAAG AAAACAGTTC 
     4201 CTCCATGTGT CTCCAGAAGT CTGGAGCCTG TGGCAGGCCA ACTAAGCAAC TCAGTGGCTA 
     4261 CCAAGCTTAC AGCTGTGGAA GGCAGCATGA AAGAGAATAT CTCTAAGCTA CTCAAGTCCA 
     4321 AGAACTTAAC AGATGCCATT GCCCGCGCAG CTGCAGATAC GCTACAGGGA CCAATGCAGG 
     4381 CTGCCTACCG AGAAGCCTTC CAGAGTGTGG TGCTGCCGGC TTTTGAGAAG AGTTGTCAGG 
     4441 CTATGTTCCA GCAAATCAAT GACAGCTTCA GGCTGGGCAC TCAGGAATAT TTGCAGCAGC 
     4501 TGGACAGTCA CATGAAGAGC CGAAAGGCAC GTGAACAAGA AGCTAGGGAG CCTGTGTTGG 
     4561 CCCAGCTTCG GGGCCTGGTC AGCACACTGC AGAATGCCAC TGAGCAGATG GCAGCCACTG 
     4621 TGTCTAGCAG TGTTCGGGCC GAGGTGCAAC ACCAGCTGCA TGTGGCTGTG GGCAGCTTGC 
     4681 AGGAATCAAT CTTAGCACAA GTACAACGCA TTGTCAAGGG TGAAGTGAGT GTGGCACTGA 
     4741 AGGAGCAGCA GGCCACTGTC ACTTCCAGCA TCATGCAGGC TATGCGCTCA GCTGCTGGCA 
     4801 CACCTGTCCC CTCTGCCCAC CTTGACTGCC AGGCCCAACA AGCCCATATC TTGCAGTTAC 
     4861 TGCAGCAGGG CCACCTCAAT CAGGCCTTCC AGCAGGCTCT GACTGCTGCT GATCTCAACC 
     4921 TGGTGCTGTA TGTGTGTGAA ACTGTGGACC CAGCCCAGGT TTTTGGGCAG CCGCCCTGTC 
     4981 CACTGTCCCA GCCCGTTCTC CTTTCCCTAA TCCAGCAGCT AGCATCTGAC CTTGGAACTA 
     5041 GAAGTGACCT CAAGCTCAGC TACTTGGAAG AGGCTGTGAT GCACCTGGAC CACAGCGATC 
     5101 CCATCACTCG AGACCACATG GGCTCTGTCA TGGCTCAGGT GCGCCAGAAG CTCTTCCAGT 
     5161 TCCTGCAGGC TGATCCACAC AACTCACTTG GCAAAGCTGC CCGCCGCCTC AGCCTAATGT 
     5221 TACACGGCCT TGTGACCCCT AGCCTCCCTA CGCGTACGCG GCCGCTCGAG CAGAAACTCA 
     5281 TCTCAGAAGA GGATCTGGCA GCAAATGATA TCCTGGATTA CAAGGATGAC GACGATAAGG 
     5341 TTTAAACGGC CGGCCGCGGT CATAGCTGTT TCCTGAACAG ATCCCGGGTG GCATCCCTGT 
     5401 GACCCCTCCC CAGTGCCTCT CCTGGCCCTG GAAGTTGCCA CTCCAGTGCC CACCAGCCTT 
     5461 GTCCTAATAA AATTAAGTTG CATCATTTTG TCTGACTAGG TGTCCTTCTA TAATATTATG 
     5521 GGGTGGAGGG GGGTGGTATG GAGCAAGGGG CAAGTTGGGA AGACAACCTG TAGGGCCTGC 
     5581 GGGGTCTATT GGGAACCAAG CTGGAGTGCA GTGGCACAAT CTTGGCTCAC TGCAATCTCC 
     5641 GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA 
     5701 TGCATGACCA GGCTCAGCTA ATTTTTGTTT TTTTGGTAGA GACGGGGTTT CACCATATTG 
     5761 GCCAGGCTGG TCTCCAACTC CTAATCTCAG GTGATCTACC CACCTTGGCC TCCCAAATTG 
     5821 CTGGGATTAC AGGCGTGAAC CACTGCTCCC TTCCCTGTCC TTCTGATTTT AAAATAACTA 
     5881 TACCAGCAGG AGGACGTCCA GACACAGCAT AGGCTACCTG GCCATGCCCA ACCGGTGGGA 
     5941 CATTTGAGTT GCTTGCTTGG CACTGTCCTC TCATGCGTTG GGTCCACTCA GTAGATGCCT 
     6001 GTTGAATTGG GTACGCGGCC AGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG 
     6061 GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA 
     6121 GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA 
     6181 CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG 
     6241 ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT 
     6301 TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG 
     6361 CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC 
     6421 CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT 
     6481 AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA 
     6541 TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC 
     6601 AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC 
     6661 TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT 
     6721 TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC 
     6781 TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT 
     6841 CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA 
     6901 ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC GACGTTGGCT GCGAGCCCTG GGCCTTCACC 
     6961 CGAACTTGGG GGGTGGGGTG GGGAAAAGGA AGAAACGCGG GCGTATTGGC CCCAATGGGG 
     7021 TCTCGGTGGG GTATCGACAG AGTGCCAGCC CTGGGACCGA ACCCCGCGTT TATGAACAAA 
     7081 CGACCCAACA CCGTGCGTTT TATTCTGTCT TTTTATTGCC GTCATAGCGC GGGTTCCTTC 
     7141 CGGTATTGTC TCCTTCCGTG TTTCAGTTAG CCTCCCCCTA GGGTGGGCGA AGAACTCCAG 
     7201 CATGAGATCC CCGCGCTGGA GGATCATCCA GCCGGCGTCC CGGAAAACGA TTCCGAAGCC 
     7261 CAACCTTTCA TAGAAGGCGG CGGTGGAATC GAAATCTCGT GATGGCAGGT TGGGCGTCGC 
     7321 TTGGTCGGTC ATTTCGAACC CCAGAGTCCC GCTCAGAAGA ACTCGTCAAG AAGGCGATAG 
     7381 AAGGCGATGC GCTGCGAATC GGGAGCGGCG ATACCGTAAA GCACGAGGAA GCGGTCAGCC 
     7441 CATTCGCCGC CAAGCTCTTC AGCAATATCA CGGGTAGCCA ACGCTATGTC CTGATAGCGA 
     7501 TCCGCCACAC CCAGCCGGCC ACAGTCGATG AATCCAGAAA AGCGGCCATT TTCCACCATG 
     7561 ATATTCGGCA AGCAGGCATC GCCATGGGTC ACGACGAGAT CCTCGCCGTC GGGCATGCTC 
     7621 GCCTTGAGCC TGGCGAACAG TTCGGCTGGC GCGAGCCCCT GATGCTCTTC GTCCAGATCA 
     7681 TCCTGATCGA CAAGACCGGC TTCCATCCGA GTACGTGCTC GCTCGATGCG ATGTTTCGCT 
     7741 TGGTGGTCGA ATGGGCAGGT AGCCGGATCA AGCGTATGCA GCCGCCGCAT TGCATCAGCC 
     7801 ATGATGGATA CTTTCTCGGC AGGAGCAAGG TGAGATGACA GGAGATCCTG CCCCGGCACT 
     7861 TCGCCCAATA GCAGCCAGTC CCTTCCCGCT TCAGTGACAA CGTCGAGCAC AGCTGCGCAA 
     7921 GGAACGCCCG TCGTGGCCAG CCACGATAGC CGCGCTGCCT CGTCTTGCAG TTCATTCAGG 
     7981 GCACCGGACA GGTCGGTCTT GACAAAAAGA ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC 
     8041 ACGGCGGCAT CAGAGCAGCC GATTGTCTGT TGTGCCCAGT CATAGCCGAA TAGCCTCTCC 
     8101 ACCCAAGCGG CCGGAGAACC TGCGTGCAAT CCATCTTGTT CAATCATGCG AAACGATCCT 
     8161 CATCCTGTCT CTTGATCGAT CTTTGCAAAA GCCTAGGCCT CCAAAAAAGC CTCCTCACTA 
     8221 CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC CTCGGCCTCT GCATAAATAA AAAAAATTAG 
     8281 TCAGCCATGG GGCGGAGAAT GGGCGGAACT GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT 
     8341 TAGGGGCGGG ACTATGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     8401 TGGGGAGCCT GGGGACTTTC CACACCTGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     8461 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCCT AACTGACACA CATTCCACAG 
     8521 CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT 
     8581 TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT 
     8641 CCGCGCACAT TTCCCCGAAA AGTGCCACCT GACGCGCCCT GTAGCGGCGC ATTAAGCGCG 
     8701 GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT 
     8761 CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA 
     8821 AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA 
     8881 CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT 
     8941 TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC 
     9001 AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG 
     9061 TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TT
//