LOCUS       RR214606                8271 bp    DNA     circular  SYN 
DEFINITION  Pelp1 (Myc-DDK-tagged ORF) - Rat proline, glutamic acid and leucine rich protein 1 (Pelp1), (10 ug)
ACCESSION   NM_001024270
VERSION     NM_001024270.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4428..4519 
                     /label="Myc-DDK"  
     misc_feature    4549..5134 
                     /label="PolyA signal"  
     misc_feature    4606..4625 
                     /label="Restriction Enzyme"  
     misc_feature    5410..6082 
                     /label="ColE1"  
     misc_feature    6512..7306 
                     /label="Kan/Neo"  
     misc_feature    7341..7643 
                     /label="SV40 ori"  
     misc_feature    7729..8206 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4410..4416 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4410 
                     /label="Pelp1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGGCAGCC GTTCTTAGTG GGCCCACCAC GGGCTCCCCT GCTGGGGCTC 
     1081 CTGGCGGGCC TGGGGGTCTC TCTGCGGCGG GATCCGGTCC GCGTTTGCGT CTGCTGCTGT 
     1141 TGGAGAGCGT TTCTGGGTTG TTGCAACCGC GAACAGGGTC GCACGTTGCC CCTGTGCATC 
     1201 CCCCAATCCA GTGGGCCCCT TATTTGCCTG GGCTCATGTG CTTACTGAGG CTGCATGGGA 
     1261 CAGCGGGTGG GGCTCAGAAT CTTTCAGCTC TTGGGGCATT GGTGAATCTC AGTAATGCAC 
     1321 ACCTTAGCTC CATCAAAACT CGGTTTGAAG GCCTGTGTCT GCTGTCCTTG TTGGTAGGGG 
     1381 AGAGCCCCAC AGAGTTGTTC CAGCAACACT GTGTTTCTTG GCTTCGGAGC ATCCAGCAGG 
     1441 TGCTGCAGTC TCAGGACTCA CCGCCCACCA TGGAGCTGGC TGTGGCTATC CTGAGGGATC 
     1501 TGCTTCGATA TGCATCCCAG CTTCCTACCC TGTTTCGGGA CATCTCCACC AACCACCTTC 
     1561 CTGGGCTTCT TACTTCCTTG CTGGGCCTCA GACCAGAGTG TGAGCAATCA GCTTTGGAGG 
     1621 GAATGAAGGC TTGTGTGACC TATTTTCCTC GGGCCTGTGG CTTTCTAAAA GGAAAGCTTG 
     1681 CTTCTTTCTT CCTGTCTCGG TTGGATTCCT TGAACCCTCA GCTCCAACAG CTGGCCTGTG 
     1741 AGTGTTACTC CAGGTTACCC TCTTTAGGGG CTGGCTTTTC CCAGGGCCTG AAGCACACAG 
     1801 AGAACTGGGA GCAGGAGCTG CACAGCCTGC TGACCTCACT GCACAGCTTG CTGGGGTCCC 
     1861 TGTTTGAGGA AACAGAGACT GCTCCTGTGC AGAGTGAAGG CCCTGGAGTA GAGATGCTGC 
     1921 TTTCACCCTC AGAAGATGAT AACACTCATG TCCTCCTCCA GCTTTGGCAG AGGTTTTCAG 
     1981 GACTGGCCCG TTGCCTGGGG CTCATGCTCA GCTCTGAGTT TGGGGCTCCT GTGTCCGTTC 
     2041 CTGTGCAGGA AATCCTGGAC CTCATCTGCC GGATCCTTGG TATCAGTAGC AAGAATATTA 
     2101 ACTTGCTTGG AGATGGTCCC CTCCGGTTGC TGCTGCTGCC CTCTCTCCAC CTTGAAGCCT 
     2161 TGGACTTGCT CTCTGCACTG ATCCTTGCGT GTGGAGGCCG ACTCTTGCGT TTTGGTGCCC 
     2221 TGATCAGCCG GCTGCTTCCC CAGGTTCTCA ACACCTGGAG CACTGGGAGG GACGCTCTGG 
     2281 CTCCAGGCCA GGAGAGGCCT TACAGCACCA TTCGGACCAA GGTGTACGCT ATCTTAGAGC 
     2341 TATGGGTGCA GGTTTGTGGG GCCTCAGCAG GTATGCTTCA GGGAGGGGCC TCTGGAGAGG 
     2401 CCTTGCTCAC CCACCTGCTC AGTGACATCT CCCCTCCAGC TGATGCTCTT AAGCTCTGCA 
     2461 GTACCAGGGG AAGCTCTGAT GGAGGTTTGC AAAGTGGGAA GCCTAGTGCC CCTAAGAAGC 
     2521 TAAAACTCGA TATGGGGGAA GCTTTGGCTC CACCCAGCCA AAGGAAAGGA GATAGGAATG 
     2581 CCAACAGCGA TGTGTGTGCA GCTGCACTGA GAGGTCTCAG CCGGACCATC CTCATGTGCG 
     2641 GGCCTCTCGT CAAGGAGGAG ACTCACAGGA GGCTTCATGA TCTCGTCCTG CCCCTGGTCA 
     2701 TGAGTGTCCA GCAGGGTGAA GTCCTGGGAA GTTCTCCTTA CAACAGCTCC TGCTGTCGCC 
     2761 TGGAGCTCTA CCGCCTGCTG CTGGCTTTAC TGCTGGCACC TTCCCCTCGC TGCCCTCCTC 
     2821 CTCTTTCCTG TGCCCTGAAA GCTTTCTCTC TTGGCCAATG GGAAGATAGT CTTGAGGTCT 
     2881 CCTCTTTCTG CTCAGAAGCT CTGGTGACTT GTTCTGCTCT GACACACCCC CGAGTACCAC 
     2941 CTCTACAGAG CTCAGGCCCT GCCTGTCCTA CACCTGCCCC CGTTCCTCCT CCTGAGGCCC 
     3001 CATCTTCATT CAGGGCCCCA GCCTTCCATA CTCCAGGTCC CATGCCCTCT ATAGGTGCTC 
     3061 TGCCATCTCC AGGCCCAGTG CCCTCAGCAG GCCCCATTCC CACAGTAGGC TCGATGTCCT 
     3121 CAGCTGGCTC TGTGCCATCC ACAGGCCCAG TGCCCTCTCG CCCCGGACCT CCAGCAACAG 
     3181 CCAACCACTT AGGGCTCGCT GTCCCAGGCC TGGTGTCTGT CCCACCCAGG CTCCTTCCTG 
     3241 GCTCTGAAAA CCACCGTGCA GGTTCTGGTG AGGATCCTGT CCTTGCTCCT AGTGGGACCC 
     3301 CTCCACCTAG CATACCTCCA GATGAAACTT TTGGGGGGAG AGTACCCAGG CCAGCCTTTG 
     3361 TCCACTACGA TAAGGAGGAG GCATCTGATG TAGAGATCTC CTTGGAAAGT GACTCTGATG 
     3421 ACAGTGTGGT GATTGTACCT GAGGGGCTTC CATCCCTGCC ACCACCACCC TCAGGCACTC 
     3481 CTCCCCCTGT GGCTCCTACT GGCCCACCAA CAGCCTCCCC TCCTGTGCCT GCTAAGGAGG 
     3541 ATTCTGAAGA ACTGCCTGCA ACTCCAGGGC CTCTACCTCC ACCCCCACCC CCACCCCCAC 
     3601 CTGTTTCTGG TCCTGTGACA CTTCCTCCAC CCCAGCTCGT CCCTGAAGGG ACTCCTGGAG 
     3661 GGGGAGGACC CACAGCTATG GAAGAAGATT TGACAGTTAT TAATATCAAC AGCAGTGATG 
     3721 AAGAGGAGGA GGAAGAAGAG GAAGAGGAGG AGGAAGATGA AGATGAGGAG GAAGAAGACT 
     3781 TTGAGGAAGA GGAAGAAGAT GAAGAAGAGT ATTTTGAAGA GGAAGAGGAG GAAGAAGAGT 
     3841 TTGAGGAGGA ATTTGAGGAG GAAGAAGGTG AATTAGAAGA GGAGGAAGAG GAGGAGGAAG 
     3901 AAGAGTTAGA AGAGGTAGAA GATGTGGAGT TCGGTTCAGC AGGGGAAGTG GAAGAAGGTG 
     3961 GACCTCCACC CCCAACACTG CCACCAGCTC TGCCACCTAC AGACTCCCCC AAAGTGCAGC 
     4021 CCGAGGCAGA ACCTGAGCCT GGGCTCTTAT TGGAAGTTGA GGAGCCAGGG GCCGAGGATG 
     4081 GGCCTGGGCC TGAGATCGCG CCCACCCTGG CCCCTGAGGT CCTCCCCTCC CAGGAGGAGG 
     4141 TGGAGCGGGA AGGGGAAAGC CCTACAGCAG GGCCACCTCA GGAGCTTGTT GAAGAAGAGT 
     4201 CCTCTGCTCC CCCTACCCTG CTGGAAGAAG GGACTGAGGG TGGAGGTGAC AAGGTGCCAC 
     4261 CCCCACCTGA GACACCTGCA CAAGAAGAGA TGGAGACAGA AACAGAAGCT TCAGCTCCTC 
     4321 AGGGAAAGGA GCAAGATGAC ACAGCTGCCA TGCTGGCTGA CTTCATTGAT TGTCCCCCTG 
     4381 ATGATGAGAA GCCACCACCT GCTACAGAGC CTGACTCTAC GCGTACGCGG CCGCTCGAGC 
     4441 AGAAACTCAT CTCAGAAGAG GATCTGGCAG CAAATGATAT CCTGGATTAC AAGGATGACG 
     4501 ACGATAAGGT TTAAACGGCC GGCCGCGGTC ATAGCTGTTT CCTGAACAGA TCCCGGGTGG 
     4561 CATCCCTGTG ACCCCTCCCC AGTGCCTCTC CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC 
     4621 ACCAGCCTTG TCCTAATAAA ATTAAGTTGC ATCATTTTGT CTGACTAGGT GTCCTTCTAT 
     4681 AATATTATGG GGTGGAGGGG GGTGGTATGG AGCAAGGGGC AAGTTGGGAA GACAACCTGT 
     4741 AGGGCCTGCG GGGTCTATTG GGAACCAAGC TGGAGTGCAG TGGCACAATC TTGGCTCACT 
     4801 GCAATCTCCG CCTCCTGGGT TCAAGCGATT CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA 
     4861 TTCCAGGCAT GCATGACCAG GCTCAGCTAA TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC 
     4921 ACCATATTGG CCAGGCTGGT CTCCAACTCC TAATCTCAGG TGATCTACCC ACCTTGGCCT 
     4981 CCCAAATTGC TGGGATTACA GGCGTGAACC ACTGCTCCCT TCCCTGTCCT TCTGATTTTA 
     5041 AAATAACTAT ACCAGCAGGA GGACGTCCAG ACACAGCATA GGCTACCTGG CCATGCCCAA 
     5101 CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC ACTGTCCTCT CATGCGTTGG GTCCACTCAG 
     5161 TAGATGCCTG TTGAATTGGG TACGCGGCCA GCGGCGAGCG GTATCAGCTC ACTCAAAGGC 
     5221 GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG 
     5281 CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG 
     5341 CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG 
     5401 ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC 
     5461 CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA 
     5521 TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT 
     5581 GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC 
     5641 CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG 
     5701 AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC 
     5761 TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT 
     5821 TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA 
     5881 GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG 
     5941 GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA 
     6001 AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT 
     6061 ATATGAGTAA CCTGAGGCTA TGGCAGGGCC TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG 
     6121 GCCTTCACCC GAACTTGGGG GGTGGGGTGG GGAAAAGGAA GAAACGCGGG CGTATTGGCC 
     6181 CCAATGGGGT CTCGGTGGGG TATCGACAGA GTGCCAGCCC TGGGACCGAA CCCCGCGTTT 
     6241 ATGAACAAAC GACCCAACAC CGTGCGTTTT ATTCTGTCTT TTTATTGCCG TCATAGCGCG 
     6301 GGTTCCTTCC GGTATTGTCT CCTTCCGTGT TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA 
     6361 GAACTCCAGC ATGAGATCCC CGCGCTGGAG GATCATCCAG CCGGCGTCCC GGAAAACGAT 
     6421 TCCGAAGCCC AACCTTTCAT AGAAGGCGGC GGTGGAATCG AAATCTCGTG ATGGCAGGTT 
     6481 GGGCGTCGCT TGGTCGGTCA TTTCGAACCC CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA 
     6541 AGGCGATAGA AGGCGATGCG CTGCGAATCG GGAGCGGCGA TACCGTAAAG CACGAGGAAG 
     6601 CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA GCAATATCAC GGGTAGCCAA CGCTATGTCC 
     6661 TGATAGCGAT CCGCCACACC CAGCCGGCCA CAGTCGATGA ATCCAGAAAA GCGGCCATTT 
     6721 TCCACCATGA TATTCGGCAA GCAGGCATCG CCATGGGTCA CGACGAGATC CTCGCCGTCG 
     6781 GGCATGCTCG CCTTGAGCCT GGCGAACAGT TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG 
     6841 TCCAGATCAT CCTGATCGAC AAGACCGGCT TCCATCCGAG TACGTGCTCG CTCGATGCGA 
     6901 TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA GCCGGATCAA GCGTATGCAG CCGCCGCATT 
     6961 GCATCAGCCA TGATGGATAC TTTCTCGGCA GGAGCAAGGT GAGATGACAG GAGATCCTGC 
     7021 CCCGGCACTT CGCCCAATAG CAGCCAGTCC CTTCCCGCTT CAGTGACAAC GTCGAGCACA 
     7081 GCTGCGCAAG GAACGCCCGT CGTGGCCAGC CACGATAGCC GCGCTGCCTC GTCTTGCAGT 
     7141 TCATTCAGGG CACCGGACAG GTCGGTCTTG ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC 
     7201 AGCCGGAACA CGGCGGCATC AGAGCAGCCG ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT 
     7261 AGCCTCTCCA CCCAAGCGGC CGGAGAACCT GCGTGCAATC CATCTTGTTC AATCATGCGA 
     7321 AACGATCCTC ATCCTGTCTC TTGATCGATC TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC 
     7381 TCCTCACTAC TTCTGGAATA GCTCAGAGGC CGAGGCGGCC TCGGCCTCTG CATAAATAAA 
     7441 AAAAATTAGT CAGCCATGGG GCGGAGAATG GGCGGAACTG GGCGGAGTTA GGGGCGGGAT 
     7501 GGGCGGAGTT AGGGGCGGGA CTATGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     7561 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCTGGTT GCTGACTAAT TGAGATGCAT 
     7621 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCCTA ACTGACACAC 
     7681 ATTCCACAGC TGGTTCTTTC CGCCTCAGGA CTCTTCCTTT TTCAATATTA TTGAAGCATT 
     7741 TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA 
     7801 ATAGGGGTTC CGCGCACATT TCCCCGAAAA GTGCCACCTG ACGCGCCCTG TAGCGGCGCA 
     7861 TTAAGCGCGG CGGGTGTGGT GGTTACGCGC AGCGTGACCG CTACACTTGC CAGCGCCCTA 
     7921 GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT 
     7981 CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG TTCCGATTTA GTGCTTTACG GCACCTCGAC 
     8041 CCCAAAAAAC TTGATTAGGG TGATGGTTCA CGTAGTGGGC CATCGCCCTG ATAGACGGTT 
     8101 TTTCGCCCTT TGACGTTGGA GTCCACGTTC TTTAATAGTG GACTCTTGTT CCAAACTGGA 
     8161 ACAACACTCA ACCCTATCTC GGTCTATTCT TTTGATTTAT AAGGGATTTT GCCGATTTCG 
     8221 GCCTATTGGT TAAAAAATGA GCTGATTTAA CAAAAATTTA ACGCGAATTT T
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