LOCUS       RR214558                8061 bp    DNA     circular  SYN 
DEFINITION  Farp2 (Myc-DDK-tagged ORF) - Rat FERM, RhoGEF and pleckstrin domain protein 2 (Farp2), (10 ug)
ACCESSION   NM_001108233
VERSION     NM_001108233.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4218..4309 
                     /label="Myc-DDK"  
     misc_feature    4339..4924 
                     /label="PolyA signal"  
     misc_feature    4396..4415 
                     /label="Restriction Enzyme"  
     misc_feature    5200..5872 
                     /label="ColE1"  
     misc_feature    6302..7096 
                     /label="Kan/Neo"  
     misc_feature    7131..7433 
                     /label="SV40 ori"  
     misc_feature    7519..7996 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4200..4206 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4200 
                     /label="Farp2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGGGGAGATA GAAGGAACAT ACAGAACTTT ACCAACGTCA GGGACACGCT 
     1081 TGGGTGGCCA GACCGCCATT GGAGTGAGTA CCCTGGAACC AGAGCAGGCC CTCTCTCCCA 
     1141 AGATGCAGGA GAAGCACATG CACATCAGAG TGAAGCTGCT GGACAGTACT GTGGAAATAT 
     1201 TTGACATTGA GCCTAAGTGT GACGGACAGG TCTTATTGAC GCAAGTGTGG AAGCATTTGA 
     1261 ATCTGATTGA ATGTGACTAC TTTGGTCTGG AGTTCAAGAA CGTCCAGTCT TACTGGATTT 
     1321 GGCTTGAACC TATGAAACCC ATCATTCGGC AAGTGCGAAA GCCTAAGAAT GCAGTGCTTC 
     1381 GCCTGGCAGT AAAATTTTTC CCACCTGACC CTGGTCAGTT GCAAGAAGAG TACACAAGGT 
     1441 ACCTGTTTGC CTTGCAACTT AGGAGAGACC TCCTAGAGGA ACGCCTGACC TGCACGGCCA 
     1501 CCACTTCCGC CCTTCTCATA TCCCACCTTC TGCAGTCGGA AATCGGAGAT TATGATGAAA 
     1561 CCCTGGATCG AGAACACCTC AAAGCCAATG ACTACTTGCC CAACCAGGAG CAATCCCTGG 
     1621 ACAAGATACT CGACTTCCAT CAAAGGCACA CGGGCCAGAC TCCTGCGGAC TCAGATTTCC 
     1681 AGGTGCTTGA GATTGCAAGA AAGTTGGAAA TGTATGGCAT CAGGTTTCAC ATGGCTTCTG 
     1741 ACAGAGAAGG GACCAAGATT AATCTGGCAG TTTCTCACAT GGGCGTCCTC GTGTTCCAGG 
     1801 GCACCACCAA AATCAATACT TTCAACTGGT CCAAAGTCCG TAAACTAAGC TTCAAGAGGA 
     1861 AAAGGTTTCT TATCAAACTC CACCCTGAGG TCCATGGGCC CTACCAGGAC ACGTTAGAGT 
     1921 TCTTGTTGGG TAGCAGAGAT GAATGTAAGA ATTTCTGGAA GATATGTGTA GAACACCACA 
     1981 CCTTTTTCAG ACTCTCTGAC CAGCCTAAGC CAAAGGCAAA AGCTGTCTTC TTCAGTCGGG 
     2041 GCTCCTCCTT CAGATACAGT GGAAGAACTC AGAAACAACT GGTAGACTAT ATCAGAGACG 
     2101 GTGGGACGAA GAGAATTCCA TACGAAAGAC GGCACAGTAA GACTCGGACG TCTCTTCATA 
     2161 CTCTGACTGG AGATCTGCCT AAACAGAGCA TCTCATTCAC CGATGGCTTG AGAACTTCTG 
     2221 CCTCCCTGTC TTCAGCAAAT GCCTCCTTTT ATCCACCCCC TACTTCTTTG TCCTCTCCTG 
     2281 GTCTGCCCAG TGTGAAGGAC AGCAGCAGCT CCCCTGTGGA TCCCCAGGCT CCCCACATCA 
     2341 AGAGCACAGC AGCAGAGAGG AGCAGCAGAC CATCATCATC TGAAGGCCCC AGCACACAGT 
     2401 CGGCCCATCT CCCTGGACCC CCTGTGCTCC AGCCTGGTCC AGGCTTTTCT GTGGATAGTC 
     2461 CTCAGCCTTC TCCCTCCAGC CTGAAGAGCC GCCTGAGCCT GAGCCCTGAG CGTCAGGCCT 
     2521 CTCTGAGCAC AGCTGAGCAG GGTTCGTCCC CTGTGCTAAG CCCTGTCCTC AGTGGCACTG 
     2581 GGACAGCCAG GATGGACAAC CAAGAAGAGC AGAAACACAA GCACACATCG GAAGACGAGG 
     2641 CCTATTTCAT AGCCAAGGAG ATTGTTGCCA CAGAGCGAAC CTACCTGAAG GACCTAGAAG 
     2701 TTATCACTGT GTGGTTCCGA AGTGTGGTGA TCAAGGAGGA GGCCATGCCT GCAGCCCTGA 
     2761 TGACCCTGCT TTTCTCCAAC ATTGATCCCA TCTACGAGTT CCATCGAGGC TTCCTTCACG 
     2821 AGGTGGAACA GAGGCTGGCA CTCTGGGAAG GGCCATCTAG CGGTGATCAT CAACGGATTG 
     2881 GGGACATCCT CCTCAGGAAC ATGCGGCAGT TAAAGGAATT TACTAGCTAC TTCCAAAGAC 
     2941 ACGATGAGGT CCTAACAGAA CTGGAAAAGG CCACAAAACA CTGTAAAAGG CTGGAGGCGG 
     3001 TCTACAAAGA GTTTGAGCTC CAGAAGGTTT GCTACCTGCC TCTCAACACG TTCCTGCTGA 
     3061 AGCCCGTCCA GAGGCTCGTG CACTACCGTC TGCTGCTAAA CCGGCTGTGT ACTCACTACT 
     3121 CACCTGAGCA CGAGGACTAC ACTGACTGCC ACGAGGCACT GAAGGCCATC ACAGAAGTGA 
     3181 CCACTGAGCT CCAGCAGAGC CTTACCCGGC TGGAAAACCT ACAGAAGCTG ACAGAGCTGC 
     3241 AGCGAGACCT GGTTGGTGTA GAAAACCTCA TCGCTCCTGG AAGGGAGTTC ATCCGTGAGG 
     3301 GCTGCCTTCA CAAGCTCACC AAGAAGGGCC TGCAACAGAG GATGTTTTTT CTGTTCTCAG 
     3361 ATATGTTGCT GTATACAAGC AAAAGTGTCA CAGGGGCCAG TCAGTTCCGG ATTCGTGGCT 
     3421 TCCTTCCACT CCGTGGCATG CTGGTAGAAG ACAGTGAGAA CGAATGGTCT GTTCCTCATT 
     3481 GCTTCACTAT CTATGCAGCT CAGAAAACAA TCGTGGTGGC AGCCAGCACT CGGCTAGAAA 
     3541 AGGAGAAGTG GATGCAGGAC CTGAATGCAG CAATCCAGGC AGCCAAGAGC GCTGGTGACA 
     3601 CACCCGCCGT GCTGCTGGGT GGCCCGGTGT ATACTCGTAG CCCCAGATCT TCTGATGAAG 
     3661 TCTCTCTGGA AGATTCAGAA GATGGTCGAG GAACCCGGGG CTCCCTAGAG GGGAACAGTC 
     3721 AGCACCGGGC CAATACCACA ATGCATGTGT GCTGGTATCG GAACACGAGT GTGTCCAGAG 
     3781 CGGACCACAG TGCAGCTGTT GAGAACCAGC TTTCAGGATA TCTGCTTAGG AAGTTCAAGA 
     3841 ACAGTAATGG CTGGCAGAAG CTCTGGGTGG TCTTTACCAA CTTCTGCTTG TTTTTCTATA 
     3901 AAACACATCA GGATGACTAC CCCCTGGCCA GCCTCCCACT ACTGGGTTAC AGTGTGAGCC 
     3961 TCCCCAGAGA GGCTGACAGC ATCCACAAAG ACTATGTTTT CAAGCTCCAA TTCAAATCTC 
     4021 ACGTCTACTT CTTCCGGGCT GAGAGCAAGT ACACATTTGA AAGGTGGATG GAAGTCATCA 
     4081 AAAGAGCCAG CAGCTCACCA GGGAGACCCC CAAGTTTCAC TCAGGACTGT TCACATCTTT 
     4141 CTCCAGGGCT GGAGGCAGAA GTCAGAGAAA AGGAGGCATG TCCTTCACTC TGCTTGGACA 
     4201 AGAACCTAAC GCGTACGCGG CCGCTCGAGC AGAAACTCAT CTCAGAAGAG GATCTGGCAG 
     4261 CAAATGATAT CCTGGATTAC AAGGATGACG ACGATAAGGT TTAAACGGCC GGCCGCGGTC 
     4321 ATAGCTGTTT CCTGAACAGA TCCCGGGTGG CATCCCTGTG ACCCCTCCCC AGTGCCTCTC 
     4381 CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC ACCAGCCTTG TCCTAATAAA ATTAAGTTGC 
     4441 ATCATTTTGT CTGACTAGGT GTCCTTCTAT AATATTATGG GGTGGAGGGG GGTGGTATGG 
     4501 AGCAAGGGGC AAGTTGGGAA GACAACCTGT AGGGCCTGCG GGGTCTATTG GGAACCAAGC 
     4561 TGGAGTGCAG TGGCACAATC TTGGCTCACT GCAATCTCCG CCTCCTGGGT TCAAGCGATT 
     4621 CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA TTCCAGGCAT GCATGACCAG GCTCAGCTAA 
     4681 TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC ACCATATTGG CCAGGCTGGT CTCCAACTCC 
     4741 TAATCTCAGG TGATCTACCC ACCTTGGCCT CCCAAATTGC TGGGATTACA GGCGTGAACC 
     4801 ACTGCTCCCT TCCCTGTCCT TCTGATTTTA AAATAACTAT ACCAGCAGGA GGACGTCCAG 
     4861 ACACAGCATA GGCTACCTGG CCATGCCCAA CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC 
     4921 ACTGTCCTCT CATGCGTTGG GTCCACTCAG TAGATGCCTG TTGAATTGGG TACGCGGCCA 
     4981 GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA 
     5041 TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC 
     5101 CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG 
     5161 CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG 
     5221 AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT 
     5281 TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT 
     5341 GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG 
     5401 CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT 
     5461 GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT 
     5521 CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT 
     5581 GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC 
     5641 CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC 
     5701 TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG 
     5761 TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA 
     5821 AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA CCTGAGGCTA TGGCAGGGCC 
     5881 TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG GCCTTCACCC GAACTTGGGG GGTGGGGTGG 
     5941 GGAAAAGGAA GAAACGCGGG CGTATTGGCC CCAATGGGGT CTCGGTGGGG TATCGACAGA 
     6001 GTGCCAGCCC TGGGACCGAA CCCCGCGTTT ATGAACAAAC GACCCAACAC CGTGCGTTTT 
     6061 ATTCTGTCTT TTTATTGCCG TCATAGCGCG GGTTCCTTCC GGTATTGTCT CCTTCCGTGT 
     6121 TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA GAACTCCAGC ATGAGATCCC CGCGCTGGAG 
     6181 GATCATCCAG CCGGCGTCCC GGAAAACGAT TCCGAAGCCC AACCTTTCAT AGAAGGCGGC 
     6241 GGTGGAATCG AAATCTCGTG ATGGCAGGTT GGGCGTCGCT TGGTCGGTCA TTTCGAACCC 
     6301 CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA AGGCGATAGA AGGCGATGCG CTGCGAATCG 
     6361 GGAGCGGCGA TACCGTAAAG CACGAGGAAG CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA 
     6421 GCAATATCAC GGGTAGCCAA CGCTATGTCC TGATAGCGAT CCGCCACACC CAGCCGGCCA 
     6481 CAGTCGATGA ATCCAGAAAA GCGGCCATTT TCCACCATGA TATTCGGCAA GCAGGCATCG 
     6541 CCATGGGTCA CGACGAGATC CTCGCCGTCG GGCATGCTCG CCTTGAGCCT GGCGAACAGT 
     6601 TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG TCCAGATCAT CCTGATCGAC AAGACCGGCT 
     6661 TCCATCCGAG TACGTGCTCG CTCGATGCGA TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA 
     6721 GCCGGATCAA GCGTATGCAG CCGCCGCATT GCATCAGCCA TGATGGATAC TTTCTCGGCA 
     6781 GGAGCAAGGT GAGATGACAG GAGATCCTGC CCCGGCACTT CGCCCAATAG CAGCCAGTCC 
     6841 CTTCCCGCTT CAGTGACAAC GTCGAGCACA GCTGCGCAAG GAACGCCCGT CGTGGCCAGC 
     6901 CACGATAGCC GCGCTGCCTC GTCTTGCAGT TCATTCAGGG CACCGGACAG GTCGGTCTTG 
     6961 ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC AGCCGGAACA CGGCGGCATC AGAGCAGCCG 
     7021 ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT AGCCTCTCCA CCCAAGCGGC CGGAGAACCT 
     7081 GCGTGCAATC CATCTTGTTC AATCATGCGA AACGATCCTC ATCCTGTCTC TTGATCGATC 
     7141 TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC TCCTCACTAC TTCTGGAATA GCTCAGAGGC 
     7201 CGAGGCGGCC TCGGCCTCTG CATAAATAAA AAAAATTAGT CAGCCATGGG GCGGAGAATG 
     7261 GGCGGAACTG GGCGGAGTTA GGGGCGGGAT GGGCGGAGTT AGGGGCGGGA CTATGGTTGC 
     7321 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     7381 ACACCTGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     7441 TGGGGACTTT CCACACCCTA ACTGACACAC ATTCCACAGC TGGTTCTTTC CGCCTCAGGA 
     7501 CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC 
     7561 ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA 
     7621 GTGCCACCTG ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG CGGGTGTGGT GGTTACGCGC 
     7681 AGCGTGACCG CTACACTTGC CAGCGCCCTA GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC 
     7741 TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG 
     7801 TTCCGATTTA GTGCTTTACG GCACCTCGAC CCCAAAAAAC TTGATTAGGG TGATGGTTCA 
     7861 CGTAGTGGGC CATCGCCCTG ATAGACGGTT TTTCGCCCTT TGACGTTGGA GTCCACGTTC 
     7921 TTTAATAGTG GACTCTTGTT CCAAACTGGA ACAACACTCA ACCCTATCTC GGTCTATTCT 
     7981 TTTGATTTAT AAGGGATTTT GCCGATTTCG GCCTATTGGT TAAAAAATGA GCTGATTTAA 
     8041 CAAAAATTTA ACGCGAATTT T
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