LOCUS       RR214501                8370 bp    DNA     circular  SYN 
DEFINITION  Rtn4 (Myc-DDK-tagged ORF) - Rat reticulon 4 (Rtn4), (10 ug)
ACCESSION   NM_031831
VERSION     NM_031831.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4527..4618 
                     /label="Myc-DDK"  
     misc_feature    4648..5233 
                     /label="PolyA signal"  
     misc_feature    4705..4724 
                     /label="Restriction Enzyme"  
     misc_feature    5509..6181 
                     /label="ColE1"  
     misc_feature    6611..7405 
                     /label="Kan/Neo"  
     misc_feature    7440..7742 
                     /label="SV40 ori"  
     misc_feature    7828..8305 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4509..4515 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4509 
                     /label="Rtn4"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAAGACATA GACCAGTCGT CGCTGGTCTC CTCGTCCACG GACAGCCCGC 
     1081 CCCGGCCTCC GCCCGCCTTC AAGTACCAGT TCGTGACGGA GCCCGAGGAC GAGGAGGACG 
     1141 AGGAGGAGGA GGAGGACGAG GAGGAGGACG ACGAGGACCT AGAGGAACTG GAGGTGCTGG 
     1201 AGAGGAAGCC CGCAGCCGGG CTGTCCGCAG CTGCGGTGCC GCCCGCCGCC GCCGCGCCGC 
     1261 TGCTGGACTT CAGCAGCGAC TCGGTGCCCC CCGCGCCCCG CGGGCCGCTG CCGGCCGCGC 
     1321 CCCCTGCCGC TCCTGAGAGG CAGCCATCCT GGGAACGCAG CCCCGCGGCG CCCGCGCCAT 
     1381 CCCTGCCGCC CGCTGCCGCA GTCCTGCCCT CCAAGCTCCC AGAGGACGAC GAGCCTCCGG 
     1441 CGAGGCCCCC GCCTCCGCCG CCAGCCGGCG CGAGCCCCCT GGCGGAGCCC GCCGCGCCCC 
     1501 CTTCCACGCC GGCCGCGCCC AAGCGCAGGG GCTCCGGCTC AGTGGATGAG ACCCTTTTTG 
     1561 CTCTTCCTGC TGCATCTGAG CCTGTGATAC CCTCCTCTGC AGAAAAAATT ATGGATTTGA 
     1621 TGGAGCAGCC AGGTAACACT GTTTCGTCTG GTCAAGAGGA TTTCCCATCT GTCCTGCTTG 
     1681 AAACTGCTGC CTCTCTTCCT TCTCTATCTC CTCTCTCAAC TGTTTCTTTT AAAGAACATG 
     1741 GATACCTTGG TAACTTATCA GCAGTGTCAT CCTCAGAAGG AACAATTGAA GAAACTTTAA 
     1801 ATGAAGCTTC TAAAGAGTTG CCAGAGAGGG CAACAAATCC ATTTGTAAAT AGAGATTTAG 
     1861 CAGAATTTTC AGAATTAGAA TATTCAGAAA TGGGATCATC TTTTAAAGGC TCCCCAAAAG 
     1921 GAGAGTCAGC CATATTAGTA GAAAACACTA AGGAAGAAGT AATTGTGAGG AGTAAAGACA 
     1981 AAGAGGATTT AGTTTGTAGT GCAGCCCTTC ACAGTCCACA AGAATCACCT GTGGGTAAAG 
     2041 AAGACAGAGT TGTGTCTCCA GAAAAGACAA TGGACATTTT TAATGAAATG CAGATGTCAG 
     2101 TAGTAGCACC TGTGAGGGAA GAGTATGCAG ACTTTAAGCC ATTTGAACAA GCATGGGAAG 
     2161 TGAAAGATAC TTATGAGGGA AGTAGGGATG TGCTGGCTGC TAGAGCTAAT GTGGAAAGTA 
     2221 AAGTGGACAG AAAATGCTTG GAAGATAGCC TGGAGCAAAA AAGTCTTGGG AAGGATAGTG 
     2281 AAGGCAGAAA TGAGGATGCT TCTTTCCCCA GTACCCCAGA ACCTGTGAAG GACAGCTCCA 
     2341 GAGCATATAT TACCTGTGCT TCCTTTACCT CAGCAACCGA AAGCACCACA GCAAACACTT 
     2401 TCCCTTTGTT AGAAGATCAT ACTTCAGAAA ATAAAACAGA TGAAAAAAAA ATAGAAGAAA 
     2461 GGAAGGCCCA AATTATAACA GAGAAGACTA GCCCCAAAAC GTCAAATCCT TTCCTTGTAG 
     2521 CAGTACAGGA TTCTGAGGCA GATTATGTTA CAACAGATAC CTTATCAAAG GTGACTGAGG 
     2581 CAGCAGTGTC AAACATGCCT GAAGGTCTGA CGCCAGATTT AGTTCAGGAA GCATGTGAAA 
     2641 GTGAACTGAA TGAAGCCACA GGTACAAAGA TTGCTTATGA AACAAAAGTG GACTTGGTCC 
     2701 AAACATCAGA AGCTATACAA GAATCACTTT ACCCCACAGC ACAGCTTTGC CCATCATTTG 
     2761 AGGAAGCTGA AGCAACTCCG TCACCAGTTT TGCCTGATAT TGTTATGGAA GCACCATTAA 
     2821 ATTCTCTCCT TCCAAGCGCT GGTGCTTCTG TAGTGCAGCC CAGTGTATCC CCACTGGAAG 
     2881 CACCTCCTCC AGTTAGTTAT GACAGTATAA AGCTTGAGCC TGAAAACCCC CCACCATATG 
     2941 AAGAAGCCAT GAATGTAGCA CTAAAAGCTT TGGGAACAAA GGAAGGAATA AAAGAGCCTG 
     3001 AAAGTTTTAA TGCAGCTGTT CAGGAAACAG AAGCTCCTTA TATATCCATT GCGTGTGATT 
     3061 TAATTAAAGA AACAAAGCTC TCCACTGAGC CAAGTCCAGA TTTCTCTAAT TATTCAGAAA 
     3121 TAGCAAAATT CGAGAAGTCG GTGCCCGAAC ACGCTGAGCT AGTGGAGGAT TCCTCACCTG 
     3181 AATCTGAACC AGTTGACTTA TTTAGTGATG ATTCGATTCC TGAAGTCCCA CAAACACAAG 
     3241 AGGAGGCTGT GATGCTCATG AAGGAGAGTC TCACTGAAGT GTCTGAGACA GTAGCCCAGC 
     3301 ACAAAGAGGA GAGACTTAGT GCCTCACCTC AGGAGCTAGG AAAGCCATAT TTAGAGTCTT 
     3361 TTCAGCCCAA TTTACATAGT ACAAAAGATG CTGCATCTAA TGACATTCCA ACATTGACCA 
     3421 AAAAGGAGAA AATTTCTTTG CAAATGGAAG AGTTTAATAC TGCAATTTAT TCAAATGATG 
     3481 ACTTACTTTC TTCTAAGGAA GACAAAATAA AAGAAAGTGA AACATTTTCA GATTCATCTC 
     3541 CGATTGAGAT AATAGATGAA TTTCCCACGT TTGTCAGTGC TAAAGATGAT TCTCCTAAAT 
     3601 TAGCCAAGGA GTACACTGAT CTAGAAGTAT CCGACAAAAG TGAAATTGCT AATATCCAAA 
     3661 GCGGGGCAGA TTCATTGCCT TGCTTAGAAT TGCCCTGTGA CCTTTCTTTC AAGAATATAT 
     3721 ATCCTAAAGA TGAAGTACAT GTTTCAGATG AATTCTCCGA AAATAGGTCC AGTGTATCTA 
     3781 AGGCATCCAT ATCGCCTTCA AATGTCTCTG CTTTGGAACC TCAGACAGAA ATGGGCAGCA 
     3841 TAGTTAAATC CAAATCACTT ACGAAAGAAG CAGAGAAAAA ACTTCCTTCT GACACAGAGA 
     3901 AAGAGGACAG ATCCCTGTCA GCTGTATTGT CAGCAGAGCT GAGTAAAACT TCAGTTGTTG 
     3961 ACCTCCTCTA CTGGAGAGAC ATTAAGAAGA CTGGAGTGGT GTTTGGTGCC AGCTTATTCC 
     4021 TGCTGCTGTC TCTGACAGTG TTCAGCATTG TCAGTGTAAC GGCCTACATT GCCTTGGCCC 
     4081 TGCTCTCGGT GACTATCAGC TTTAGGATAT ATAAGGGCGT GATCCAGGCT ATCCAGAAAT 
     4141 CAGATGAAGG CCACCCATTC AGGGCATATT TAGAATCTGA AGTTGCTATA TCAGAGGAAT 
     4201 TGGTTCAGAA ATACAGTAAT TCTGCTCTTG GTCATGTGAA CAGCACAATA AAAGAACTGA 
     4261 GGCGGCTTTT CTTAGTTGAT GATTTAGTTG ATTCCCTGAA GTTTGCAGTG TTGATGTGGG 
     4321 TGTTTACTTA TGTTGGTGCC TTGTTCAATG GTCTGACACT ACTGATTTTA GCTCTGATCT 
     4381 CACTCTTCAG TATTCCTGTT ATTTATGAAC GGCATCAGGT GCAGATAGAT CATTATCTAG 
     4441 GACTTGCAAA CAAGAGTGTT AAGGATGCCA TGGCCAAAAT CCAAGCAAAA ATCCCTGGAT 
     4501 TGAAGCGCAA AGCAGATACG CGTACGCGGC CGCTCGAGCA GAAACTCATC TCAGAAGAGG 
     4561 ATCTGGCAGC AAATGATATC CTGGATTACA AGGATGACGA CGATAAGGTT TAAACGGCCG 
     4621 GCCGCGGTCA TAGCTGTTTC CTGAACAGAT CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA 
     4681 GTGCCTCTCC TGGCCCTGGA AGTTGCCACT CCAGTGCCCA CCAGCCTTGT CCTAATAAAA 
     4741 TTAAGTTGCA TCATTTTGTC TGACTAGGTG TCCTTCTATA ATATTATGGG GTGGAGGGGG 
     4801 GTGGTATGGA GCAAGGGGCA AGTTGGGAAG ACAACCTGTA GGGCCTGCGG GGTCTATTGG 
     4861 GAACCAAGCT GGAGTGCAGT GGCACAATCT TGGCTCACTG CAATCTCCGC CTCCTGGGTT 
     4921 CAAGCGATTC TCCTGCCTCA GCCTCCCGAG TTGTTGGGAT TCCAGGCATG CATGACCAGG 
     4981 CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA CGGGGTTTCA CCATATTGGC CAGGCTGGTC 
     5041 TCCAACTCCT AATCTCAGGT GATCTACCCA CCTTGGCCTC CCAAATTGCT GGGATTACAG 
     5101 GCGTGAACCA CTGCTCCCTT CCCTGTCCTT CTGATTTTAA AATAACTATA CCAGCAGGAG 
     5161 GACGTCCAGA CACAGCATAG GCTACCTGGC CATGCCCAAC CGGTGGGACA TTTGAGTTGC 
     5221 TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG TCCACTCAGT AGATGCCTGT TGAATTGGGT 
     5281 ACGCGGCCAG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA 
     5341 ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG 
     5401 TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA 
     5461 AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT 
     5521 TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT 
     5581 GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT 
     5641 CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC 
     5701 CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT 
     5761 ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC 
     5821 TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGAACAG TATTTGGTAT 
     5881 CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA 
     5941 ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA 
     6001 AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA 
     6061 AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT 
     6121 TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAC CTGAGGCTAT 
     6181 GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC GAGCCCTGGG CCTTCACCCG AACTTGGGGG 
     6241 GTGGGGTGGG GAAAAGGAAG AAACGCGGGC GTATTGGCCC CAATGGGGTC TCGGTGGGGT 
     6301 ATCGACAGAG TGCCAGCCCT GGGACCGAAC CCCGCGTTTA TGAACAAACG ACCCAACACC 
     6361 GTGCGTTTTA TTCTGTCTTT TTATTGCCGT CATAGCGCGG GTTCCTTCCG GTATTGTCTC 
     6421 CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG GTGGGCGAAG AACTCCAGCA TGAGATCCCC 
     6481 GCGCTGGAGG ATCATCCAGC CGGCGTCCCG GAAAACGATT CCGAAGCCCA ACCTTTCATA 
     6541 GAAGGCGGCG GTGGAATCGA AATCTCGTGA TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT 
     6601 TTCGAACCCC AGAGTCCCGC TCAGAAGAAC TCGTCAAGAA GGCGATAGAA GGCGATGCGC 
     6661 TGCGAATCGG GAGCGGCGAT ACCGTAAAGC ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA 
     6721 AGCTCTTCAG CAATATCACG GGTAGCCAAC GCTATGTCCT GATAGCGATC CGCCACACCC 
     6781 AGCCGGCCAC AGTCGATGAA TCCAGAAAAG CGGCCATTTT CCACCATGAT ATTCGGCAAG 
     6841 CAGGCATCGC CATGGGTCAC GACGAGATCC TCGCCGTCGG GCATGCTCGC CTTGAGCCTG 
     6901 GCGAACAGTT CGGCTGGCGC GAGCCCCTGA TGCTCTTCGT CCAGATCATC CTGATCGACA 
     6961 AGACCGGCTT CCATCCGAGT ACGTGCTCGC TCGATGCGAT GTTTCGCTTG GTGGTCGAAT 
     7021 GGGCAGGTAG CCGGATCAAG CGTATGCAGC CGCCGCATTG CATCAGCCAT GATGGATACT 
     7081 TTCTCGGCAG GAGCAAGGTG AGATGACAGG AGATCCTGCC CCGGCACTTC GCCCAATAGC 
     7141 AGCCAGTCCC TTCCCGCTTC AGTGACAACG TCGAGCACAG CTGCGCAAGG AACGCCCGTC 
     7201 GTGGCCAGCC ACGATAGCCG CGCTGCCTCG TCTTGCAGTT CATTCAGGGC ACCGGACAGG 
     7261 TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC TGCGCTGACA GCCGGAACAC GGCGGCATCA 
     7321 GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA TAGCCGAATA GCCTCTCCAC CCAAGCGGCC 
     7381 GGAGAACCTG CGTGCAATCC ATCTTGTTCA ATCATGCGAA ACGATCCTCA TCCTGTCTCT 
     7441 TGATCGATCT TTGCAAAAGC CTAGGCCTCC AAAAAAGCCT CCTCACTACT TCTGGAATAG 
     7501 CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC ATAAATAAAA AAAATTAGTC AGCCATGGGG 
     7561 CGGAGAATGG GCGGAACTGG GCGGAGTTAG GGGCGGGATG GGCGGAGTTA GGGGCGGGAC 
     7621 TATGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     7681 GGACTTTCCA CACCTGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     7741 TGGGGAGCCT GGGGACTTTC CACACCCTAA CTGACACACA TTCCACAGCT GGTTCTTTCC 
     7801 GCCTCAGGAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG 
     7861 AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT 
     7921 CCCCGAAAAG TGCCACCTGA CGCGCCCTGT AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG 
     7981 GTTACGCGCA GCGTGACCGC TACACTTGCC AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC 
     8041 TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC 
     8101 CCTTTAGGGT TCCGATTTAG TGCTTTACGG CACCTCGACC CCAAAAAACT TGATTAGGGT 
     8161 GATGGTTCAC GTAGTGGGCC ATCGCCCTGA TAGACGGTTT TTCGCCCTTT GACGTTGGAG 
     8221 TCCACGTTCT TTAATAGTGG ACTCTTGTTC CAAACTGGAA CAACACTCAA CCCTATCTCG 
     8281 GTCTATTCTT TTGATTTATA AGGGATTTTG CCGATTTCGG CCTATTGGTT AAAAAATGAG 
     8341 CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//