LOCUS       RR214495                7803 bp    DNA     circular  SYN 
DEFINITION  Hltf (Myc-DDK-tagged ORF) - Rat helicase-like transcription factor (Hltf), (10 ug)
ACCESSION   NM_001106478
VERSION     NM_001106478.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3960..4051 
                     /label="Myc-DDK"  
     misc_feature    4081..4666 
                     /label="PolyA signal"  
     misc_feature    4138..4157 
                     /label="Restriction Enzyme"  
     misc_feature    4942..5614 
                     /label="ColE1"  
     misc_feature    6044..6838 
                     /label="Kan/Neo"  
     misc_feature    6873..7175 
                     /label="SV40 ori"  
     misc_feature    7261..7738 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3942..3948 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3942 
                     /label="Hltf"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTCTTGGAAG TTCAAGAGGG ATTCTGTTTG GAAGTATTCA CAGTCTGCCC 
     1081 AGTATGGAGC CCATGAAAAT ATTCCAAGAC TTTCATACCC ACCTTTCTTT CCACGTTTTG 
     1141 AATTCCAAGA CATTATCCCT CCAGATGACT TCCAAACTAG TGATGAAGAG CTAGATTTGG 
     1201 TTTTATTTGG AACTATGAGA GGACAAGTTG TTGGAATACG CTATTACACA GGAGTTGTGA 
     1261 ATAACAATGA AATGGTTGCT TTAGAACGAG AGCCTAATAA CCCGTATGAT AAGAATGCTA 
     1321 TTAAAGTAAA TAATGTGAAT GGAAATCAGG TTGGCCATCT AAAGAGAGAT CTTGCAGCTG 
     1381 CCTTGGCCTA TATCATGGAC AACAAATTGG CCCAAGTGGA AGGGGTAGTT CCTTTTGGTG 
     1441 CAAGTAATAC TTTTACCATG CCTCTTCATA TGACTTTCTG GGGAAAAGAA GAAAATAGAG 
     1501 AGGTGGTTTT AGATCATTTG AAGAAACATG GATTTAAGTT GGGTCCCACA CCAAAAACTG 
     1561 TAGGATCGAG TTTGGAGAAT ACATGGGGCT CTGGAAGAGC TGGACCAAGC TATAGTAGGC 
     1621 CGGTTCATGT TGCTGTACAG ATGACAACTG AACAGCTCAA AACGGAATTT GACAAATTAT 
     1681 TTGAAGATTT AAAAGAAGAC GATAGAACTA TTGAAATGGA GCCAGCTGAG GCTGTTGAAA 
     1741 CACCATTGCT CCCACACCAA AAACAAGCTC TAGCTTGGAT GATTGCACGA GAGAACAGCA 
     1801 AAGAACTTCC ACCCTTCTGG GAACTGCGAA ATGACCTGTA TTATAATACA ATAACAAATT 
     1861 TTTCTGTGAA GGAACGACCA GAAAATGTTC ATGGAGGAAT TTTAGCTGAT GATATGGGTT 
     1921 TGGGCAAAAC TCTTACTGCT ATTGCTGTAA TTCTTACCAA CTTTGATGAT GGCAGGCCGC 
     1981 TTCTTGGTAA AAGAGGCAAG GAGAGTCACC CAGGAAAGGA ATACAAGGAC GAGACCGTTA 
     2041 AACTCAGAGG AAGTAATACG AACAAGACGA CAGATGGACA CTCAGAGCCA TCAGCATGTA 
     2101 GTGAAGAACC CAGTATTTCA GATGTTTCGG AGAAGAGTAA TGTGTCAGAT TTGTTTAGTT 
     2161 TCTTCTCTAA AAGAAGAAAA ATTTCTGTAC AGTCCACTGA AAGCAGTGAA TCAGAGGAAA 
     2221 TTGAAACAAG TAAACTGTCC CAGAAAATGA AAGGCAAGCT GAAAAATACA CAGTTAGATA 
     2281 CTAAAAGTAA AGTAAAAGGT ACATCTGTGA TGGAGAGCTC AAAGAAGTGT GACACTGGAG 
     2341 AGAGGACAAG AACAACACTG ATCATCTGTC CACTCTCTGT GCTGAGCAAT TGGATTGACC 
     2401 AGATTGGACA ACATATAAAA TCAGAAGTGG ACTTGAATCT TTATGTTTAT TATGGTCCTG 
     2461 ATCGTATTAG AGATTCAACC TGGCTTTCAA AACAGGATAT TATTTTGACT ACTTATAATA 
     2521 TTTTAACTCA TGATTATGGA ACTAAAGATA ATAGTCCATT ACACAGCATA AAGTGGTTAA 
     2581 GAGTGATCCT GGATGAAGGC CATGCCATAC GAAATCCAAA TGCTCAACAG ACAAAAGCTG 
     2641 TTCTTGAGTT AGAGGCAGAA AGAAGATGGG TATTGACAGG TACTCCTATC CAGAATTCTT 
     2701 TGAAGGACTT GTGGTCTCTT CTGTCCTTTT TAAAACTGAA ACCATTTACT GATAGAGAAT 
     2761 GGTGGCATAG AATAATACAG CGTCCTGTCA CAACAGGAGA TGAGGGAGGA CTTAGGCGTT 
     2821 TACAGTCCTT AATTAAAAGC ATCACACTTA GAAGAACAAA GACAAGCAAA ATTAAAGGGA 
     2881 AACCTGTCTT GGAGTTACCA GAAAGGAAAG TATTTATTCA GCACATTACT CTGTCAGTCG 
     2941 AAGAGAGAAA GATTTATCAA TCTGTTAAAA ATGAAGGCAA AGCTACTATT GCAAGGTATT 
     3001 TTACTGAAGG TACTGTCCTT GCACACTATG CAGATGTCTT GGGTCTTCTG CTTAGACTGC 
     3061 GGCAGATTTG TTGTCATGTC CATCTTCCTA CAAATGGAAC GTCTTCAAGT GACCCCTCCA 
     3121 GAAGCGACAC ACCTGAAGAA CTGAGGAAGA TGTTAGTAAC GAAGATGAAG TTAATTCTGA 
     3181 GCTCTGGTTC AGATGAAGAA TGTTCAATTT GCCTGGATTC ATTAACATTT CCTGTGATCA 
     3241 CACATTGTGC ACATGTGTTT TGTAAACCTT GCATTTGCCA GGTCATTCAG CGCGAGCAGC 
     3301 CACATGCAAA GTGTCCTTTA TGCAGAAGTA ATATACATGG ACATAATTTA TTAGAATGTC 
     3361 CTCCAGAAGA ATTGGCATGT GACAGTGACA ACAAGTCCGA TATGGAATGG ACGTCCAGTT 
     3421 CAAAGATTAA TGCTCTAATG AATGCTTTGA TTGAGTTACG GACAAAGGAC CCCAACATAA 
     3481 AAAGTTTAGT TGTCTCTCAG TTTACCACAT TCCTGTCTTT AATAGAAACA CCACTGAAAG 
     3541 CCTCAGGATT TGTGTTTACT CGCTTAGACG GTTCCATGGC TCAAAAGAAA AGAGTTGAAT 
     3601 CAATTCAGTG TTTTCAAAAT ACAGACGCAG GATCACCTAC TATAATGCTA CTGTCCTTAA 
     3661 AAGCAGGCGG AGTCGGCTTG AATCTTTGTG CAGCTTCTCG AGTGTTCTTA ATGGACCCAG 
     3721 CCTGGAATCC TGCTGCAGAG GATCAGTGCT TTGACCGATG CCATCGACTT GGCCAGAAAC 
     3781 AAGAGGTTAT CATTACAAAG TTCATTGTGA AGGATTCTGT TGAAGAAAAT ATGTTGAAAA 
     3841 TACAAAACAT GAAAAGAGAG CTTGCAGCCG GAGCCTTTGG AACTAAGAAA ACAGATGCTG 
     3901 ATGAAATGAA ACAAGCTAAA ATCAATGAAA TCAGAACTTT AATTGATCTA ACGCGTACGC 
     3961 GGCCGCTCGA GCAGAAACTC ATCTCAGAAG AGGATCTGGC AGCAAATGAT ATCCTGGATT 
     4021 ACAAGGATGA CGACGATAAG GTTTAAACGG CCGGCCGCGG TCATAGCTGT TTCCTGAACA 
     4081 GATCCCGGGT GGCATCCCTG TGACCCCTCC CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC 
     4141 ACTCCAGTGC CCACCAGCCT TGTCCTAATA AAATTAAGTT GCATCATTTT GTCTGACTAG 
     4201 GTGTCCTTCT ATAATATTAT GGGGTGGAGG GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG 
     4261 AAGACAACCT GTAGGGCCTG CGGGGTCTAT TGGGAACCAA GCTGGAGTGC AGTGGCACAA 
     4321 TCTTGGCTCA CTGCAATCTC CGCCTCCTGG GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC 
     4381 GAGTTGTTGG GATTCCAGGC ATGCATGACC AGGCTCAGCT AATTTTTGTT TTTTTGGTAG 
     4441 AGACGGGGTT TCACCATATT GGCCAGGCTG GTCTCCAACT CCTAATCTCA GGTGATCTAC 
     4501 CCACCTTGGC CTCCCAAATT GCTGGGATTA CAGGCGTGAA CCACTGCTCC CTTCCCTGTC 
     4561 CTTCTGATTT TAAAATAACT ATACCAGCAG GAGGACGTCC AGACACAGCA TAGGCTACCT 
     4621 GGCCATGCCC AACCGGTGGG ACATTTGAGT TGCTTGCTTG GCACTGTCCT CTCATGCGTT 
     4681 GGGTCCACTC AGTAGATGCC TGTTGAATTG GGTACGCGGC CAGCGGCGAG CGGTATCAGC 
     4741 TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT 
     4801 GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT 
     4861 CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG 
     4921 AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC 
     4981 TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT 
     5041 GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA 
     5101 GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA 
     5161 TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA 
     5221 CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA 
     5281 CTACGGCTAC ACTAGAAGAA CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT 
     5341 CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT 
     5401 TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT 
     5461 CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT 
     5521 GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC 
     5581 AATCTAAAGT ATATATGAGT AACCTGAGGC TATGGCAGGG CCTGCCGCCC CGACGTTGGC 
     5641 TGCGAGCCCT GGGCCTTCAC CCGAACTTGG GGGGTGGGGT GGGGAAAAGG AAGAAACGCG 
     5701 GGCGTATTGG CCCCAATGGG GTCTCGGTGG GGTATCGACA GAGTGCCAGC CCTGGGACCG 
     5761 AACCCCGCGT TTATGAACAA ACGACCCAAC ACCGTGCGTT TTATTCTGTC TTTTTATTGC 
     5821 CGTCATAGCG CGGGTTCCTT CCGGTATTGT CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT 
     5881 AGGGTGGGCG AAGAACTCCA GCATGAGATC CCCGCGCTGG AGGATCATCC AGCCGGCGTC 
     5941 CCGGAAAACG ATTCCGAAGC CCAACCTTTC ATAGAAGGCG GCGGTGGAAT CGAAATCTCG 
     6001 TGATGGCAGG TTGGGCGTCG CTTGGTCGGT CATTTCGAAC CCCAGAGTCC CGCTCAGAAG 
     6061 AACTCGTCAA GAAGGCGATA GAAGGCGATG CGCTGCGAAT CGGGAGCGGC GATACCGTAA 
     6121 AGCACGAGGA AGCGGTCAGC CCATTCGCCG CCAAGCTCTT CAGCAATATC ACGGGTAGCC 
     6181 AACGCTATGT CCTGATAGCG ATCCGCCACA CCCAGCCGGC CACAGTCGAT GAATCCAGAA 
     6241 AAGCGGCCAT TTTCCACCAT GATATTCGGC AAGCAGGCAT CGCCATGGGT CACGACGAGA 
     6301 TCCTCGCCGT CGGGCATGCT CGCCTTGAGC CTGGCGAACA GTTCGGCTGG CGCGAGCCCC 
     6361 TGATGCTCTT CGTCCAGATC ATCCTGATCG ACAAGACCGG CTTCCATCCG AGTACGTGCT 
     6421 CGCTCGATGC GATGTTTCGC TTGGTGGTCG AATGGGCAGG TAGCCGGATC AAGCGTATGC 
     6481 AGCCGCCGCA TTGCATCAGC CATGATGGAT ACTTTCTCGG CAGGAGCAAG GTGAGATGAC 
     6541 AGGAGATCCT GCCCCGGCAC TTCGCCCAAT AGCAGCCAGT CCCTTCCCGC TTCAGTGACA 
     6601 ACGTCGAGCA CAGCTGCGCA AGGAACGCCC GTCGTGGCCA GCCACGATAG CCGCGCTGCC 
     6661 TCGTCTTGCA GTTCATTCAG GGCACCGGAC AGGTCGGTCT TGACAAAAAG AACCGGGCGC 
     6721 CCCTGCGCTG ACAGCCGGAA CACGGCGGCA TCAGAGCAGC CGATTGTCTG TTGTGCCCAG 
     6781 TCATAGCCGA ATAGCCTCTC CACCCAAGCG GCCGGAGAAC CTGCGTGCAA TCCATCTTGT 
     6841 TCAATCATGC GAAACGATCC TCATCCTGTC TCTTGATCGA TCTTTGCAAA AGCCTAGGCC 
     6901 TCCAAAAAAG CCTCCTCACT ACTTCTGGAA TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC 
     6961 TGCATAAATA AAAAAAATTA GTCAGCCATG GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT 
     7021 TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG GACTATGGTT GCTGACTAAT TGAGATGCAT 
     7081 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCTGG TTGCTGACTA 
     7141 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCC 
     7201 TAACTGACAC ACATTCCACA GCTGGTTCTT TCCGCCTCAG GACTCTTCCT TTTTCAATAT 
     7261 TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG 
     7321 AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC TGACGCGCCC 
     7381 TGTAGCGGCG CATTAAGCGC GGCGGGTGTG GTGGTTACGC GCAGCGTGAC CGCTACACTT 
     7441 GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT TTCTTCCCTT CCTTTCTCGC CACGTTCGCC 
     7501 GGCTTTCCCC GTCAAGCTCT AAATCGGGGG CTCCCTTTAG GGTTCCGATT TAGTGCTTTA 
     7561 CGGCACCTCG ACCCCAAAAA ACTTGATTAG GGTGATGGTT CACGTAGTGG GCCATCGCCC 
     7621 TGATAGACGG TTTTTCGCCC TTTGACGTTG GAGTCCACGT TCTTTAATAG TGGACTCTTG 
     7681 TTCCAAACTG GAACAACACT CAACCCTATC TCGGTCTATT CTTTTGATTT ATAAGGGATT 
     7741 TTGCCGATTT CGGCCTATTG GTTAAAAAAT GAGCTGATTT AACAAAAATT TAACGCGAAT 
     7801 TTT
//