LOCUS       RR214440                7593 bp    DNA     circular  SYN 
DEFINITION  Tlr12 (Myc-DDK-tagged ORF) - Rat toll-like receptor 12 (Tlr12), (10 ug)
ACCESSION   NM_001108682
VERSION     NM_001108682.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3750..3841 
                     /label="Myc-DDK"  
     misc_feature    3871..4456 
                     /label="PolyA signal"  
     misc_feature    3928..3947 
                     /label="Restriction Enzyme"  
     misc_feature    4732..5404 
                     /label="ColE1"  
     misc_feature    5834..6628 
                     /label="Kan/Neo"  
     misc_feature    6663..6965 
                     /label="SV40 ori"  
     misc_feature    7051..7528 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3732..3738 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3732 
                     /label="Tlr12"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGGCAGGTCC TTCCTGCTGC CCGGTCTCCT CCTTTCACTG CCTCTGGTAA 
     1081 CAGGGTGGAC CACTCCCAAA TGCCTGGTGA CTGAAGGCTC CCAACTGCCC CTGGTCTCCC 
     1141 GCTATTTCAC ACTCTGCCAC TACTCCAAGC TGTCCTTTCT TGCTGCATGC TTCCCTGTGA 
     1201 GCAACCTGAC GCAGACCTTG GAAGCTGTGC CTCGGAATGT GGAGGGGCTC TGCCTCAGTG 
     1261 GTTCTGTGTC TACTCTGCTT CCGGATGCCT TCTCTGCTTT CCCTGGACTC AAGTTCCTGG 
     1321 GACTGAATCT GCACCTTACC CGACTCCTGC CAGGAGCTCT CCGGGGCCTG GGACAGTTAC 
     1381 GGAACCTCTC TTTTGTTGAC CAACCTTCTG GAAAGAACTC TCTCTTTCTA CCTCCTGATG 
     1441 CCTTTGGTGA CCTGATTTCC CTCCAGAGAC TCCATTTCTG TGGCCCCTGT CTGAATAAGA 
     1501 AGGCAGGTGT CCGTCTACCT TCCAGTCTGC AATGGCTCTC TGTCACAATC AGTTGCCTTC 
     1561 AGGACTCAGG AGAGCTGGCT GGTATATTCC CAGATCTGGT GCAAAATTCC TCCTCCAGGG 
     1621 CTTCGTGGAC CCTGAAGAAG TTGGATCTGT CATTAAACCA GAAGCTGAAG ATGGCTACTC 
     1681 CTGGGTCCCT ACAGGGTCTC CAGGTGGAGA TTCTGGACCT GAGGAAAACA CAACTGGATG 
     1741 CTGGGGCAGT GAAGGGCCTG GGACTTCAGA AACTCAATGT TTTGTATGCA CCGACTGCCA 
     1801 CGGCCGAGCT GGCTGCTGAG ACTGCTGCCC ACTTTGAGCT GCAGGGCCTG AATGTGGACA 
     1861 GAAGCAAGAT AGGAAATATA TCTCAGGAGG CCCTGGCTTC CTGCCATAGC CTGGAGACCT 
     1921 TGAGTCTGTC AGACACTGGA CTGACTAAGC TGCCACCAGG CTTTCTGGCT GCCATGCCTA 
     1981 GGCTTCGGAG ACTGAACCTG GCCGGAAACC AACTACAGAG CACCATGCTG TGTATGAATG 
     2041 AGACAGGAGA CGTGTCAGGA CTCTCAACTC TGGATCTGTC AGGCAATGGG TTGCGCATCC 
     2101 TGCCTCCAGC CACCTTCTCC TGTTTACCAC ACTTGCGAGA GCTGCTGCTT CAGGACAACC 
     2161 AGCTGCTTTC CCTGGAGGGG CACCCGTTCC AAGATCTACA GCAACTAGAG ACCTTGAAGC 
     2221 TGGATAGGAA CCCCCTGCTT AACCTGGGGA AGAACTGTTT GGCTGCTCTG CCTGCATTGA 
     2281 CTACCCTTAG CCTGCTAGAT ACCCAAATAT TGCACAGCCC AGATGCTGGC TTCTGGGGAG 
     2341 CAAGGAGTCT GCATACCTTA CACCTGAGCT TGCCCCCTCT CTCTGCTCCG GCAGTATTGT 
     2401 CCCTGCCCAT GTATCTGACC AGTTTAGAGC TTCATGTAAC CCCAGGCTTG AAGCATTGGA 
     2461 CACTGTCCCC AAATATCTTT CCTTTCTTGG AGACCTTGAC CATAAACGGC AGGGGACTGA 
     2521 AGCTGGGGGT CCAAAATGCC TCTGAGGTCT TCCCTGCTCT CCAGCATCTC TTTCTGCTCC 
     2581 AGAATAGCTT GGATGCCTTC TGCTCCCAGG ACGCCTCCAG CATTTTTCTC TGGCAGCTCC 
     2641 CCAAACTTCA GTCCTTGAAG GTATGGGGTG CTGGAAGTAA TTCCAGACCC TGCCTCATCA 
     2701 CTGGGCTGCC CAGCCTGCAG GAGCTAAAGC TGGAGTCTCT ACAGTCCATC ACCCAGCCCC 
     2761 GCTCAGTACA GCTGGAGGAG CTGGTGGGTG ACCTTCCACA ACTCCAGGCG TTACAGCTGT 
     2821 CCAGCACAGG GCTCAAATCC CTGTCGGCTG CTGCTTTCCG GCGCCTGCAC AGTCTCCAGG 
     2881 CCTTAGTGCT GGACAGTGAG AAAGACTTGG TGCTGCAGGA CAGTCTGAGG GAGTACAGCC 
     2941 CTCAGATGCC CCGCTATGTA TACATTCTGC AGTCAAAGTT GGCCTGCCAG TGTGCTAATG 
     3001 CGTGGATGGA GCTGTGGGTT AAGCAGTCCA CCAAAACGTA CGTACACATA AGAGACGGTC 
     3061 ACCTGTGCCC AGGAGAAGTC AGGGTCCCCG CCAGGGATTC CCTTATCTCC TTTCTCTGGG 
     3121 ACCACTGCCC CCAGACTTTG GAGCTGAAAC TCTTTTTGGC CAGTTCTGCC TTGGTGTTAT 
     3181 TGCTAATTGT CTTGCCTCTC CTCCAAGGAG CCAGGAACAC TTGGATCCCC TACCTGCGGG 
     3241 CCTTGTTCAG GATTTGGCTC CAGGGTCTGA GGGGTCAGGG GAATGCGGGG AAGAGGTTCC 
     3301 TTTTTGATGT ATTCGTGTCC CACTGCAGGC AAGACCAGGG CTGGGTGCTA GAGGAACTTC 
     3361 TGCCTGCTCT GGAGGGCTTC CTTCCAGCTG GCCTCGGCCT ACGCCTCTGT CTCCCCGAGC 
     3421 GTGACTTTGA GCCTGGTAAG GATGTAGTTG ATAATGTGGT AGACAGCATG GTGAGCAGCC 
     3481 GAGTCACGCT CTGCGTGCTG AGTGGCCCGG CCTTGTGTAA CCCCCGATGC TGCCTGGAGC 
     3541 TCCGCTTGGC CACTTCTCTT CTCCTGGCTG CCCCGTCCCC TCCAGTGTTG CTGCTGGTCT 
     3601 TCCTGGAACC CATTTCCCGA CACCAGCTCC CCAGTTACCA CAGACTGGCT CGGTTGCTTC 
     3661 GACGAGGAGA CTACTGCCTG TGGCCTGAGG AGGAGGAAAG AAAGGGTGGG TTCTGGACGT 
     3721 GGCTGAGGAG CAGGCTAGGG ACGCGTACGC GGCCGCTCGA GCAGAAACTC ATCTCAGAAG 
     3781 AGGATCTGGC AGCAAATGAT ATCCTGGATT ACAAGGATGA CGACGATAAG GTTTAAACGG 
     3841 CCGGCCGCGG TCATAGCTGT TTCCTGAACA GATCCCGGGT GGCATCCCTG TGACCCCTCC 
     3901 CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC ACTCCAGTGC CCACCAGCCT TGTCCTAATA 
     3961 AAATTAAGTT GCATCATTTT GTCTGACTAG GTGTCCTTCT ATAATATTAT GGGGTGGAGG 
     4021 GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG AAGACAACCT GTAGGGCCTG CGGGGTCTAT 
     4081 TGGGAACCAA GCTGGAGTGC AGTGGCACAA TCTTGGCTCA CTGCAATCTC CGCCTCCTGG 
     4141 GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC GAGTTGTTGG GATTCCAGGC ATGCATGACC 
     4201 AGGCTCAGCT AATTTTTGTT TTTTTGGTAG AGACGGGGTT TCACCATATT GGCCAGGCTG 
     4261 GTCTCCAACT CCTAATCTCA GGTGATCTAC CCACCTTGGC CTCCCAAATT GCTGGGATTA 
     4321 CAGGCGTGAA CCACTGCTCC CTTCCCTGTC CTTCTGATTT TAAAATAACT ATACCAGCAG 
     4381 GAGGACGTCC AGACACAGCA TAGGCTACCT GGCCATGCCC AACCGGTGGG ACATTTGAGT 
     4441 TGCTTGCTTG GCACTGTCCT CTCATGCGTT GGGTCCACTC AGTAGATGCC TGTTGAATTG 
     4501 GGTACGCGGC CAGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC 
     4561 AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA 
     4621 CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA 
     4681 CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC 
     4741 GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA 
     4801 CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA 
     4861 TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA 
     4921 GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA 
     4981 CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG 
     5041 TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG 
     5101 TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG 
     5161 CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG 
     5221 AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA 
     5281 CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT 
     5341 CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AACCTGAGGC 
     5401 TATGGCAGGG CCTGCCGCCC CGACGTTGGC TGCGAGCCCT GGGCCTTCAC CCGAACTTGG 
     5461 GGGGTGGGGT GGGGAAAAGG AAGAAACGCG GGCGTATTGG CCCCAATGGG GTCTCGGTGG 
     5521 GGTATCGACA GAGTGCCAGC CCTGGGACCG AACCCCGCGT TTATGAACAA ACGACCCAAC 
     5581 ACCGTGCGTT TTATTCTGTC TTTTTATTGC CGTCATAGCG CGGGTTCCTT CCGGTATTGT 
     5641 CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT AGGGTGGGCG AAGAACTCCA GCATGAGATC 
     5701 CCCGCGCTGG AGGATCATCC AGCCGGCGTC CCGGAAAACG ATTCCGAAGC CCAACCTTTC 
     5761 ATAGAAGGCG GCGGTGGAAT CGAAATCTCG TGATGGCAGG TTGGGCGTCG CTTGGTCGGT 
     5821 CATTTCGAAC CCCAGAGTCC CGCTCAGAAG AACTCGTCAA GAAGGCGATA GAAGGCGATG 
     5881 CGCTGCGAAT CGGGAGCGGC GATACCGTAA AGCACGAGGA AGCGGTCAGC CCATTCGCCG 
     5941 CCAAGCTCTT CAGCAATATC ACGGGTAGCC AACGCTATGT CCTGATAGCG ATCCGCCACA 
     6001 CCCAGCCGGC CACAGTCGAT GAATCCAGAA AAGCGGCCAT TTTCCACCAT GATATTCGGC 
     6061 AAGCAGGCAT CGCCATGGGT CACGACGAGA TCCTCGCCGT CGGGCATGCT CGCCTTGAGC 
     6121 CTGGCGAACA GTTCGGCTGG CGCGAGCCCC TGATGCTCTT CGTCCAGATC ATCCTGATCG 
     6181 ACAAGACCGG CTTCCATCCG AGTACGTGCT CGCTCGATGC GATGTTTCGC TTGGTGGTCG 
     6241 AATGGGCAGG TAGCCGGATC AAGCGTATGC AGCCGCCGCA TTGCATCAGC CATGATGGAT 
     6301 ACTTTCTCGG CAGGAGCAAG GTGAGATGAC AGGAGATCCT GCCCCGGCAC TTCGCCCAAT 
     6361 AGCAGCCAGT CCCTTCCCGC TTCAGTGACA ACGTCGAGCA CAGCTGCGCA AGGAACGCCC 
     6421 GTCGTGGCCA GCCACGATAG CCGCGCTGCC TCGTCTTGCA GTTCATTCAG GGCACCGGAC 
     6481 AGGTCGGTCT TGACAAAAAG AACCGGGCGC CCCTGCGCTG ACAGCCGGAA CACGGCGGCA 
     6541 TCAGAGCAGC CGATTGTCTG TTGTGCCCAG TCATAGCCGA ATAGCCTCTC CACCCAAGCG 
     6601 GCCGGAGAAC CTGCGTGCAA TCCATCTTGT TCAATCATGC GAAACGATCC TCATCCTGTC 
     6661 TCTTGATCGA TCTTTGCAAA AGCCTAGGCC TCCAAAAAAG CCTCCTCACT ACTTCTGGAA 
     6721 TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC TGCATAAATA AAAAAAATTA GTCAGCCATG 
     6781 GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG 
     6841 GACTATGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     6901 TGGGGACTTT CCACACCTGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     6961 TGCTGGGGAG CCTGGGGACT TTCCACACCC TAACTGACAC ACATTCCACA GCTGGTTCTT 
     7021 TCCGCCTCAG GACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC 
     7081 ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA 
     7141 TTTCCCCGAA AAGTGCCACC TGACGCGCCC TGTAGCGGCG CATTAAGCGC GGCGGGTGTG 
     7201 GTGGTTACGC GCAGCGTGAC CGCTACACTT GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT 
     7261 TTCTTCCCTT CCTTTCTCGC CACGTTCGCC GGCTTTCCCC GTCAAGCTCT AAATCGGGGG 
     7321 CTCCCTTTAG GGTTCCGATT TAGTGCTTTA CGGCACCTCG ACCCCAAAAA ACTTGATTAG 
     7381 GGTGATGGTT CACGTAGTGG GCCATCGCCC TGATAGACGG TTTTTCGCCC TTTGACGTTG 
     7441 GAGTCCACGT TCTTTAATAG TGGACTCTTG TTCCAAACTG GAACAACACT CAACCCTATC 
     7501 TCGGTCTATT CTTTTGATTT ATAAGGGATT TTGCCGATTT CGGCCTATTG GTTAAAAAAT 
     7561 GAGCTGATTT AACAAAAATT TAACGCGAAT TTT
//