LOCUS       RR214390                8490 bp    DNA     circular  SYN 
DEFINITION  Slc12a2 (Myc-DDK-tagged ORF) - Rat solute carrier family 12 (sodium/potassium/chloride transporters), member 2 (Slc12a2), (10 ug)
ACCESSION   NM_031798
VERSION     NM_031798.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4647..4738 
                     /label="Myc-DDK"  
     misc_feature    4768..5353 
                     /label="PolyA signal"  
     misc_feature    4825..4844 
                     /label="Restriction Enzyme"  
     misc_feature    5629..6301 
                     /label="ColE1"  
     misc_feature    6731..7525 
                     /label="Kan/Neo"  
     misc_feature    7560..7862 
                     /label="SV40 ori"  
     misc_feature    7948..8425 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4629..4635 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4629 
                     /label="Slc12a2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAGCCCGGG CCCGCGGCGC CCTCGTCTGG CGCCCCGCGG CCCGCCCGGG 
     1081 ATGGGGACGC GCCGCTGACT GCCGCCGGGG TGGACCTGCC CGGCACGGCC GTACTCTCCG 
     1141 GTCGAGAGGA TGCTACCGCT GGGAGCCAGG CCGGCGGCGG GGTGCGCGGA GAGGGAACCC 
     1201 CGGCCGCTGG CGATGGGCTG GGCAAACCTC TGGGGCCCAC CCCCAGCCAG AGCCGCTTCC 
     1261 AGGTGGACCC GGTTTCCGAG AACGCCGGGC GGGCCGCGGC TGCTGCAGCT GCAGCGGCGG 
     1321 CTGCGGCGGC TGCCGGGGCA GCGGGCAAGG AGACCCCCGC GGCTGGGAAA GCCGGCACTG 
     1381 AGAGCGGCGT GGCCAAGGGC AGCGAGGAAG CCAAGGGCCG CTTCCGCGTG AACTTCGTGG 
     1441 ACCCGGCTGC CTCCTCGTCG GCGGACGACA GCCTGTCGGA TGCGGCGGGA GTCGGTGGCG 
     1501 ACGGGCCCAA CGTGAGCTCC CAGAACGGCG GGGACACGGT GCTGAGCGAG GGCAGCAGCC 
     1561 TGCACTCGGG TGGTGGCAGT GGACACCACC AGCAGTACTA CTATGACACC CACACCAACA 
     1621 CTTACTACCT GCGCACCTTC GGCCACAACA CCATGGACGC GGTGCCCAGG ATCGACCACT 
     1681 ACCGGCACAC GGCCGCGCAG CTGGGCGAGA AGCTGCTCCG GCCCAGCCTG GGAGAGTTCC 
     1741 ACGATGAGCT GGAAAAGGAA CCTTTTGAGG ATGGTTTTGC GAATGGAGAA GAAAGTACTC 
     1801 CAACCAGAGA TGCTGTGGTC ACATACACTG CCGAAAGTAA AGGAGTTGTA AAGTTTGGCT 
     1861 GGATCAAGGG TGTTTTAGTA CGATGTATGT TGAATATTTG GGGTGTGATG CTCTTTATTC 
     1921 GATTGTCATG GATTGTGGGT CAGGCTGGAA TAGGTCTCTC CGTTCTTGTA ATAGCAATGG 
     1981 CCACTGTTGT GACAACCATC ACAGGATTGT CTACTTCAGC AATAGCTACC AATGGATTCG 
     2041 TGAGAGGAGG AGGAGCATAC TATTTAATCT CTAGAAGTCT AGGGCCAGAA TTTGGCGGTG 
     2101 CGATTGGCTT GATCTTCGCT TTTCCCAATG CAGTGGCAGT TGCTATGTAT GTTGTTGGAT 
     2161 TCGCAGAGAC TGTAGTGGAG CTGCTTAAGG AACATTCCAT ACTTATGATA GATGAAATCA 
     2221 ATGACATCCG AATTATTGGA GCCATTACAG TGGTGGTTCT TCTGGGCATC TCAGTGGCCG 
     2281 GAATGGAGTG GGAAGCAAAG GCTCAGATCG TCCTTCTGGT GATTCTTCTT CTGGCCATCG 
     2341 CTGACTTCGT CATAGGAACA TTTATCTCAC TGGACAGCAA GAAGCCCAAG GGTTTCTTTG 
     2401 GCTATAAATC TGAAATATTT AGTGAGAACT TTGGACCGGA TTTTCGAGAA GAAGAGACTT 
     2461 TCTTTTCTGT ATTCGCCATC TTTTTTCCTG CTGCAACTGG TATTCTAGCT GGGGCGAACA 
     2521 TCTCGGGTGA CCTTGCAGAT CCTCAGTCAG CCATACCCAA AGGAACGCTC TTAGCCATTT 
     2581 TGATTACGAC AGTGGTTTAC ATAGGAATCG CAGTGTCTGT AGGTTCTTGT GTTGTCCGGG 
     2641 ATGCCACTGG GAACGTTAAT GACACCATTA CAACAGAGCT AACTAACTGT ACTTCTGCAG 
     2701 CCTGCAAATT AAACTTTGAC TTTTCCTACT GTGAAAGCAA CACCTGTTCT TATGGGCTAA 
     2761 TGAACAACTT TCAGGTGATG AGCATGGTGT CAGGATTTGC ACCCCTGATC TCTGCGGGTA 
     2821 TCTTTTCAGC CACACTCTCA TCAGCACTGG CATCTCTTGT GAGTGCTCCC AAAATATTTC 
     2881 AGGCTCTATG TAAGGACAAT ATCTACCCAG CTTTCCAGAT GTTTGCGAAA GGTTATGGAA 
     2941 AAAATAATGA ACCTCTTCGT GGTTACATCT TAACATTCTT AATTGCACTT GGATTCATCT 
     3001 TAATTGCTGA ATTGAATGTC ATTGCTCCAA TTATCTCTAA TTTCTTTCTC GCATCATACG 
     3061 CCTTAATCAA CTTTTCTGTA TTCCATGCGT CACTTGCAAA GTCTCCAGGG TGGCGTCCTG 
     3121 CGTTCAAGTA CTACAACATG TGGATCTCCC TTACTGGAGC GATTCTCTGC TGCATAGTGA 
     3181 TGTTCGTCAT TAACTGGTGG GCTGCACTGC TCACATATGT GATAGTTCTT GGACTGTACA 
     3241 TTTATGTCAC TTACAAAAAG CCAGATGTGA ACTGGGGATC CTCCACACAA GCGCTGACTT 
     3301 ACCTCAGCGC ACTGCAGCAT TCAATCCGTC TTTCCGGGGT GGAGGACCAT GTGAAAAACT 
     3361 TCAGGCCACA GTGTCTTGTA ATGACAGGTT CTCCAAACTC ACGGCCTGCT TTACTGCATC 
     3421 TTGTTCATGA CTTCACGAAA AATGTTGGTT TAATGATCTG TGGCCATGTG CATATGGGTC 
     3481 CCCGAAGACA AGCTATGAAA GAGATGTCCA TTGATCAAGC CAAATATCAG CGATGGCTCA 
     3541 TTAAAAACAA AATGAAGGCT TTCTACGCTC CGGTTCACGC AGATGACTTG CGGGAAGGTG 
     3601 CACAGTACCT GATGCAGGCT GCTGGACTTG GTCGTATGAA ACCAAACACA CTTGTCCTCG 
     3661 GATTTAAGAA AGATTGGTTA CAAGCAGATA TGAGGGATGT GGATATGTAT ATAAACTTAT 
     3721 TTCATGATGC TTTTGACATA CAGTATGGAG TTGTGGTCAT TCGCCTAAAG GAAGGACTGG 
     3781 ATATATCACA CCTCCAAGGA CAAGAAGAAT TATTGTCATC ACAAGAAAAG TCACCTGGTA 
     3841 CCAAGGATGT GGTAGTAAAT GTGGACTACA GTAAGAAGTC AGATCAAGAT GCTTTCAAAG 
     3901 CATCTGGTGA AAAACCTATT ACACAGAAAG ATGAGGAAGA GGATGGCAAG ACTTCAACTC 
     3961 AGCCACTGTT GAAAAAAGAA TCCAAGGGCC CCGTGGCACC TTTAAATGTA GCTGACCAAA 
     4021 AGCTTCTTGA AGCTAGCACA CAGTTTCAGA AAAAACAAGG GAAGAATACT ATAGATGTCT 
     4081 GGTGGCTTTT TGATGATGGA GGTTTGACCT TGTTGATACC CTATCTTCTG ACAACCAAGA 
     4141 AAAAGTGGAA AGATTGTAAG ATCCGAGTAT TCATTGGTGG AAAGATAAAC AGAATAGACC 
     4201 ATGACCGGAG AGCGATGGCT ACTTTACTCA GTAAATTCCG AATAGACTTC TCCGATATCA 
     4261 TGGTCCTAGG AGACATCAAC ACCAAACCAA AAAAGGAAAA CATCGTCGCT TTTGATGACA 
     4321 TGATTGAGCC ATACAGACTT CACGAGGATG ATAAAGAGCA GGATATTGCA GACAAAATGA 
     4381 AAGAGGATGA GCCCTGGCGC ATCACAGACA ACGAGCTGGA GCTCTACAAG ACCAAGACAT 
     4441 ATCGGCAGAT CAGGTTAAAT GAATTATTAA AGGAGCATTC AAGCACAGCT AATATTATTG 
     4501 TCATGAGTCT TCCAGTTGCC CGGAAGGGTG CTGTGTCCAG CGCGCTGTAC ATGGCTTGGT 
     4561 TAGAAGCTCT GTCTAAGGAC CTGCCACCAA TCCTGCTGGT TCGTGGGAAT CATCAGAGCG 
     4621 TCCTTACCTT CTACTCTACG CGTACGCGGC CGCTCGAGCA GAAACTCATC TCAGAAGAGG 
     4681 ATCTGGCAGC AAATGATATC CTGGATTACA AGGATGACGA CGATAAGGTT TAAACGGCCG 
     4741 GCCGCGGTCA TAGCTGTTTC CTGAACAGAT CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA 
     4801 GTGCCTCTCC TGGCCCTGGA AGTTGCCACT CCAGTGCCCA CCAGCCTTGT CCTAATAAAA 
     4861 TTAAGTTGCA TCATTTTGTC TGACTAGGTG TCCTTCTATA ATATTATGGG GTGGAGGGGG 
     4921 GTGGTATGGA GCAAGGGGCA AGTTGGGAAG ACAACCTGTA GGGCCTGCGG GGTCTATTGG 
     4981 GAACCAAGCT GGAGTGCAGT GGCACAATCT TGGCTCACTG CAATCTCCGC CTCCTGGGTT 
     5041 CAAGCGATTC TCCTGCCTCA GCCTCCCGAG TTGTTGGGAT TCCAGGCATG CATGACCAGG 
     5101 CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA CGGGGTTTCA CCATATTGGC CAGGCTGGTC 
     5161 TCCAACTCCT AATCTCAGGT GATCTACCCA CCTTGGCCTC CCAAATTGCT GGGATTACAG 
     5221 GCGTGAACCA CTGCTCCCTT CCCTGTCCTT CTGATTTTAA AATAACTATA CCAGCAGGAG 
     5281 GACGTCCAGA CACAGCATAG GCTACCTGGC CATGCCCAAC CGGTGGGACA TTTGAGTTGC 
     5341 TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG TCCACTCAGT AGATGCCTGT TGAATTGGGT 
     5401 ACGCGGCCAG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA 
     5461 ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG 
     5521 TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA 
     5581 AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT 
     5641 TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT 
     5701 GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT 
     5761 CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC 
     5821 CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT 
     5881 ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC 
     5941 TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGAACAG TATTTGGTAT 
     6001 CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA 
     6061 ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA 
     6121 AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA 
     6181 AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT 
     6241 TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAC CTGAGGCTAT 
     6301 GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC GAGCCCTGGG CCTTCACCCG AACTTGGGGG 
     6361 GTGGGGTGGG GAAAAGGAAG AAACGCGGGC GTATTGGCCC CAATGGGGTC TCGGTGGGGT 
     6421 ATCGACAGAG TGCCAGCCCT GGGACCGAAC CCCGCGTTTA TGAACAAACG ACCCAACACC 
     6481 GTGCGTTTTA TTCTGTCTTT TTATTGCCGT CATAGCGCGG GTTCCTTCCG GTATTGTCTC 
     6541 CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG GTGGGCGAAG AACTCCAGCA TGAGATCCCC 
     6601 GCGCTGGAGG ATCATCCAGC CGGCGTCCCG GAAAACGATT CCGAAGCCCA ACCTTTCATA 
     6661 GAAGGCGGCG GTGGAATCGA AATCTCGTGA TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT 
     6721 TTCGAACCCC AGAGTCCCGC TCAGAAGAAC TCGTCAAGAA GGCGATAGAA GGCGATGCGC 
     6781 TGCGAATCGG GAGCGGCGAT ACCGTAAAGC ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA 
     6841 AGCTCTTCAG CAATATCACG GGTAGCCAAC GCTATGTCCT GATAGCGATC CGCCACACCC 
     6901 AGCCGGCCAC AGTCGATGAA TCCAGAAAAG CGGCCATTTT CCACCATGAT ATTCGGCAAG 
     6961 CAGGCATCGC CATGGGTCAC GACGAGATCC TCGCCGTCGG GCATGCTCGC CTTGAGCCTG 
     7021 GCGAACAGTT CGGCTGGCGC GAGCCCCTGA TGCTCTTCGT CCAGATCATC CTGATCGACA 
     7081 AGACCGGCTT CCATCCGAGT ACGTGCTCGC TCGATGCGAT GTTTCGCTTG GTGGTCGAAT 
     7141 GGGCAGGTAG CCGGATCAAG CGTATGCAGC CGCCGCATTG CATCAGCCAT GATGGATACT 
     7201 TTCTCGGCAG GAGCAAGGTG AGATGACAGG AGATCCTGCC CCGGCACTTC GCCCAATAGC 
     7261 AGCCAGTCCC TTCCCGCTTC AGTGACAACG TCGAGCACAG CTGCGCAAGG AACGCCCGTC 
     7321 GTGGCCAGCC ACGATAGCCG CGCTGCCTCG TCTTGCAGTT CATTCAGGGC ACCGGACAGG 
     7381 TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC TGCGCTGACA GCCGGAACAC GGCGGCATCA 
     7441 GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA TAGCCGAATA GCCTCTCCAC CCAAGCGGCC 
     7501 GGAGAACCTG CGTGCAATCC ATCTTGTTCA ATCATGCGAA ACGATCCTCA TCCTGTCTCT 
     7561 TGATCGATCT TTGCAAAAGC CTAGGCCTCC AAAAAAGCCT CCTCACTACT TCTGGAATAG 
     7621 CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC ATAAATAAAA AAAATTAGTC AGCCATGGGG 
     7681 CGGAGAATGG GCGGAACTGG GCGGAGTTAG GGGCGGGATG GGCGGAGTTA GGGGCGGGAC 
     7741 TATGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     7801 GGACTTTCCA CACCTGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     7861 TGGGGAGCCT GGGGACTTTC CACACCCTAA CTGACACACA TTCCACAGCT GGTTCTTTCC 
     7921 GCCTCAGGAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG 
     7981 AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT 
     8041 CCCCGAAAAG TGCCACCTGA CGCGCCCTGT AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG 
     8101 GTTACGCGCA GCGTGACCGC TACACTTGCC AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC 
     8161 TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC 
     8221 CCTTTAGGGT TCCGATTTAG TGCTTTACGG CACCTCGACC CCAAAAAACT TGATTAGGGT 
     8281 GATGGTTCAC GTAGTGGGCC ATCGCCCTGA TAGACGGTTT TTCGCCCTTT GACGTTGGAG 
     8341 TCCACGTTCT TTAATAGTGG ACTCTTGTTC CAAACTGGAA CAACACTCAA CCCTATCTCG 
     8401 GTCTATTCTT TTGATTTATA AGGGATTTTG CCGATTTCGG CCTATTGGTT AAAAAATGAG 
     8461 CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//