LOCUS       RR214355                8331 bp    DNA     circular  SYN 
DEFINITION  Ppp2r3a (Myc-DDK-tagged ORF) - Rat protein phosphatase 2 (formerly 2A), regulatory subunit B'', alpha (Ppp2r3a), (10 ug)
ACCESSION   NM_001012202
VERSION     NM_001012202.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4488..4579 
                     /label="Myc-DDK"  
     misc_feature    4609..5194 
                     /label="PolyA signal"  
     misc_feature    4666..4685 
                     /label="Restriction Enzyme"  
     misc_feature    5470..6142 
                     /label="ColE1"  
     misc_feature    6572..7366 
                     /label="Kan/Neo"  
     misc_feature    7401..7703 
                     /label="SV40 ori"  
     misc_feature    7789..8266 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4470..4476 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4470 
                     /label="Ppp2r3a"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCAGCAACT TACAGACTCG TGGTTAGCAC TGTGAACCAT TATAGCAGTG 
     1081 TAGTGATAGA CCGGCGTTTT GAACAAGCCA TACATTATTG CACTGGAACC TGCCATACCT 
     1141 TCACACATGG AATAGACTGC ATTGTAGTGC ATCATAGTGT TTGTGCAGAC CTTTTGCACA 
     1201 TCCCTGTGTC TCAGTTCAAA GATGTGGATC TGAACTCTGT GTTTTTGTCT CATGAAAATG 
     1261 GCCTTTCCTC AGCTGAAGAC TATCCCCATC AGGCCCTCAC AGGAATACCC AGGTCCAAGA 
     1321 GAGCATCCAC ATTTCAGAAT ACCTGTAACT TAAAGGACAT TGCAGGAGAA GCAATCAGCT 
     1381 TTGCCAGTGG GAAAATAAAA GAATTTTCCC TTGAAAAACT CAGAAATTCA AATCATGCAG 
     1441 CTTACAAAAA AGGAAGAAAA GTTAAATCTG ACTCATTTAA TAGGCGATCA GTTGATTTGG 
     1501 ATTTACTCTG TGGCCATTAT AGTAACGATG GGACCTCCCC ATCCTTTGGC TTACTCCGGA 
     1561 GTTCCTCAGT TGAGGAAAAA TCTTTATCTC ATAGAAACTC ACTAGATACA AGCCTAACTT 
     1621 CCATGCTTTT TCAGAACTTG TCTGAAGAAG ATCTAGTTAC GCAGATTTTG GAAAAACATA 
     1681 AAATAGATCA TTTTTCTTCT GGGACAGACA TAAAAATGTG CTTGGACATC TTACTGAAAT 
     1741 GCTCTGAAGA TTTGAAAAAA TGTACAGACA TCATAAAACA ATGCATAAAG AAAAAGTCAG 
     1801 GAGGAAGCAT CAACGAAGGA AGTGGCAGTG ACGCAATGTC TAACTCTGAA ACCGTCTATA 
     1861 TGAATGTAAT GGCCAGGTTA GCTTACTACC TGAGAAAGTT ACCATTTGAA TTTGTGCAGT 
     1921 CTGGGAGTAA TGAGACTGTA GATTTGACAG AGCTTGTCAA TAATGTACCT AACTTACAAC 
     1981 TGACTCCATT CTCCCCATTA TATGGGACTG AACAGCCCCC TAAATATGAA GATATCGTCC 
     2041 ACCTCTCAGC CCTTGACTCC AGACAGTTTC AGACTGTTGA ATTGCAAGAT GACATGCATA 
     2101 ATTTTAAACA AACAGATACT GTAAAAACCC CTCCAAACAA CACAAATTCC TTGTGTAGCT 
     2161 TGGAGGTAAA CGATCCTGGA ACTCTAAAAC CTGTGCAGCT TCAATCACCG TCATTAACCA 
     2221 TAAACCATTT AGAGAATGTT TCTTCTGGTA ACTTAATGGA AACTCTTTAT ATTGAAGAAG 
     2281 AGTCAGATGG AAAGAAGTCA TTATTAGATA AAGGACAAAA AGCAGATAAT GGACAAGGTC 
     2341 AGGAACCATT AAAGGTAAAT GAACATAAAG AATTTTCAGA CCATGATACT TATCTTAAAA 
     2401 AATGTCCTGC CCCAGTGCAA AATGAAATCA GTAAGATATT TGAAAAGCCA ATGGTAGTTC 
     2461 GTCCACCTAA GGAAGAATCT GAATTAGCAG TTCCTCCTAC AGAACTCAGA GCCCAGAGGC 
     2521 ACTTTATGAA TCCTAACAGT CAAGAAGAGA TCGACAAATT GTTAATGGAT TTGGAATCAT 
     2581 TCTCACAGAA GATGGAGACC TCTCTAGGAG AACCACTTGC CAAGGGTAAA AGCATCAATT 
     2641 CTCTAAATAG TCATAGTCAT TTGACTGGTC AGATCCACAA AGATCTTGAG CCTAAACCTA 
     2701 TAGTCTCTTC AGCTACGGAG AAAGTCTCAC CTTCTTGTCT AACAAGGATT ATTGAAAACA 
     2761 ACGGACACAA AATAGAGGAA GAGGACCGAG CCCTCTTACT TCGAATTCTG GAAAGCATTG 
     2821 AAGACTTTGC TCAAGAACTA GTTGAATGCA AGTCAAGCAG AGGGAGCCTA TCACAAGAAA 
     2881 AGGAAATGAT GCAAATTCTG CAGGAAACTT TGACAGCTTC CTCCCAGCCC ACTCCATCAG 
     2941 TCAGTAGGAG CCCTGTTGGT GAGAAACCCA AAGATAGTAC TTCAGTTGTT TTGATTCAAC 
     3001 AGACACCAGA AGTGATCAAG ATTCAAAATA AACCAGAAAA AAAACCTGCA ACACCATGTC 
     3061 CACCTGCAGC CACCCTTCCA AGTAGTCCCC AGCCTGCCTC CCCTGTTCCT TGTGTGAGCG 
     3121 ATGTCGTGAA TACACCACTG TCCATAAACA TTCCACAGTT CTACTTTCCC GAAGGACTCC 
     3181 CAGACACCTG CAGCAACCAC GAACAGATCC TAGGCAGAAT TGAAGCTGCT TTCAAGGATA 
     3241 TTGAAGATCA AAAAGCAGAT ATTCATGAAA TGGGGAGAAT TGCAAAGGCC TGCGGCTGTC 
     3301 CACTCTATTG GAAAGCCCCC ATGTTCAGGG CTGCAGGAGG AGAGAAGACT GGATTTGTGA 
     3361 CAGCACAGTC ATTCATGGCC ATGTGGAAAA AGTTGCTAAG TGACCATCAT GATGATGCCT 
     3421 CCAGGTTCAT CTGGCTTCTG GCAAAGCCCA GCTGCAGCCA TCTCCAACAA GAGGATTTTA 
     3481 TCCCTCTACT TCAGGATGTG GTAGATACAC ACCCTGGCCT CACCTTCCTG AAAGATGCTC 
     3541 CTGAATTCCA TTCCCGCTAC ATCACCACGG TTGTTCAGAG AATATTCTAC ACAGTCAACA 
     3601 GATCTTGGAG TGGAAAAATT ACTTCAACAG AGATAAGGAA AAGCAACTTT TTGCAAACTC 
     3661 TGGCCCTTTT GGAGGAAGAG GAAGATATAA ACCAAATCAC AGATTACTTC TCTTATGAAC 
     3721 ATTTCTATGT CATTTATTGT AAATTCTGGG AACTAGATGG TGATCATGAC CTATACATCA 
     3781 GCCAAGCTGA TCTCTCTCGA TACAATGACC AGGCTTCATC AAATAGAATT ATTGAAAGAA 
     3841 TATTTTCTGG AGCAGTAACA AGAGGGAAAA CTGTACAAAG AGAGGGAAGA ATGAGCTATG 
     3901 CAGATTTTGT TTGGTTTTTG ATTTCTGAGG AAGACAAACG GAACCCTACC AGCATTGAGT 
     3961 ATTGGTTCCG CTGCATGGAT GTGGATGGAG ATGGTGTACT TTCCATGTAT GAACTAGAGT 
     4021 ATTTCTATGA AGAGCAGTGT GAACGGATGG AAGCCATGGG CATCGAGCCC TTGCCGTTTC 
     4081 ATGATCTGTT GTGCCAGATG CTAGACTTGG TGAAGCCAGC TAATGATGGC AGAATAACTC 
     4141 TGCGAGATTT GAAGAGGTGC AGAATGGCTC ACATCTTCTA CGACACTTTC TTTAACCTGG 
     4201 AGAAGTACTT GGACCATGAA CAAAGAGATC CTTTTGCAGT CCAGAAGGAT GTTGAAAATG 
     4261 AGGGCCCCGA GCCCTCTGAC TGGGACAGGT TTGCTGCTGA GGAGTACGAG GCTCTGGTGA 
     4321 CTGAGGAATC TGCCCAGGTT CAGCTCCAGG AAGGCTCATT TGAAGACTAT GAATCAGATG 
     4381 AGCCTGTGTC TCCCTCTGAA CTTGGAAACA AGGGCAATAA AATCCTAACT TCAAGCCTTT 
     4441 CAGAGAAATG TGGAAAGCTC CAGTCAGTGG ATGAAGAGAC GCGTACGCGG CCGCTCGAGC 
     4501 AGAAACTCAT CTCAGAAGAG GATCTGGCAG CAAATGATAT CCTGGATTAC AAGGATGACG 
     4561 ACGATAAGGT TTAAACGGCC GGCCGCGGTC ATAGCTGTTT CCTGAACAGA TCCCGGGTGG 
     4621 CATCCCTGTG ACCCCTCCCC AGTGCCTCTC CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC 
     4681 ACCAGCCTTG TCCTAATAAA ATTAAGTTGC ATCATTTTGT CTGACTAGGT GTCCTTCTAT 
     4741 AATATTATGG GGTGGAGGGG GGTGGTATGG AGCAAGGGGC AAGTTGGGAA GACAACCTGT 
     4801 AGGGCCTGCG GGGTCTATTG GGAACCAAGC TGGAGTGCAG TGGCACAATC TTGGCTCACT 
     4861 GCAATCTCCG CCTCCTGGGT TCAAGCGATT CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA 
     4921 TTCCAGGCAT GCATGACCAG GCTCAGCTAA TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC 
     4981 ACCATATTGG CCAGGCTGGT CTCCAACTCC TAATCTCAGG TGATCTACCC ACCTTGGCCT 
     5041 CCCAAATTGC TGGGATTACA GGCGTGAACC ACTGCTCCCT TCCCTGTCCT TCTGATTTTA 
     5101 AAATAACTAT ACCAGCAGGA GGACGTCCAG ACACAGCATA GGCTACCTGG CCATGCCCAA 
     5161 CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC ACTGTCCTCT CATGCGTTGG GTCCACTCAG 
     5221 TAGATGCCTG TTGAATTGGG TACGCGGCCA GCGGCGAGCG GTATCAGCTC ACTCAAAGGC 
     5281 GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG 
     5341 CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG 
     5401 CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG 
     5461 ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC 
     5521 CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA 
     5581 TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT 
     5641 GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC 
     5701 CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG 
     5761 AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC 
     5821 TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT 
     5881 TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA 
     5941 GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG 
     6001 GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA 
     6061 AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT 
     6121 ATATGAGTAA CCTGAGGCTA TGGCAGGGCC TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG 
     6181 GCCTTCACCC GAACTTGGGG GGTGGGGTGG GGAAAAGGAA GAAACGCGGG CGTATTGGCC 
     6241 CCAATGGGGT CTCGGTGGGG TATCGACAGA GTGCCAGCCC TGGGACCGAA CCCCGCGTTT 
     6301 ATGAACAAAC GACCCAACAC CGTGCGTTTT ATTCTGTCTT TTTATTGCCG TCATAGCGCG 
     6361 GGTTCCTTCC GGTATTGTCT CCTTCCGTGT TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA 
     6421 GAACTCCAGC ATGAGATCCC CGCGCTGGAG GATCATCCAG CCGGCGTCCC GGAAAACGAT 
     6481 TCCGAAGCCC AACCTTTCAT AGAAGGCGGC GGTGGAATCG AAATCTCGTG ATGGCAGGTT 
     6541 GGGCGTCGCT TGGTCGGTCA TTTCGAACCC CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA 
     6601 AGGCGATAGA AGGCGATGCG CTGCGAATCG GGAGCGGCGA TACCGTAAAG CACGAGGAAG 
     6661 CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA GCAATATCAC GGGTAGCCAA CGCTATGTCC 
     6721 TGATAGCGAT CCGCCACACC CAGCCGGCCA CAGTCGATGA ATCCAGAAAA GCGGCCATTT 
     6781 TCCACCATGA TATTCGGCAA GCAGGCATCG CCATGGGTCA CGACGAGATC CTCGCCGTCG 
     6841 GGCATGCTCG CCTTGAGCCT GGCGAACAGT TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG 
     6901 TCCAGATCAT CCTGATCGAC AAGACCGGCT TCCATCCGAG TACGTGCTCG CTCGATGCGA 
     6961 TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA GCCGGATCAA GCGTATGCAG CCGCCGCATT 
     7021 GCATCAGCCA TGATGGATAC TTTCTCGGCA GGAGCAAGGT GAGATGACAG GAGATCCTGC 
     7081 CCCGGCACTT CGCCCAATAG CAGCCAGTCC CTTCCCGCTT CAGTGACAAC GTCGAGCACA 
     7141 GCTGCGCAAG GAACGCCCGT CGTGGCCAGC CACGATAGCC GCGCTGCCTC GTCTTGCAGT 
     7201 TCATTCAGGG CACCGGACAG GTCGGTCTTG ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC 
     7261 AGCCGGAACA CGGCGGCATC AGAGCAGCCG ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT 
     7321 AGCCTCTCCA CCCAAGCGGC CGGAGAACCT GCGTGCAATC CATCTTGTTC AATCATGCGA 
     7381 AACGATCCTC ATCCTGTCTC TTGATCGATC TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC 
     7441 TCCTCACTAC TTCTGGAATA GCTCAGAGGC CGAGGCGGCC TCGGCCTCTG CATAAATAAA 
     7501 AAAAATTAGT CAGCCATGGG GCGGAGAATG GGCGGAACTG GGCGGAGTTA GGGGCGGGAT 
     7561 GGGCGGAGTT AGGGGCGGGA CTATGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     7621 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCTGGTT GCTGACTAAT TGAGATGCAT 
     7681 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCCTA ACTGACACAC 
     7741 ATTCCACAGC TGGTTCTTTC CGCCTCAGGA CTCTTCCTTT TTCAATATTA TTGAAGCATT 
     7801 TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA 
     7861 ATAGGGGTTC CGCGCACATT TCCCCGAAAA GTGCCACCTG ACGCGCCCTG TAGCGGCGCA 
     7921 TTAAGCGCGG CGGGTGTGGT GGTTACGCGC AGCGTGACCG CTACACTTGC CAGCGCCCTA 
     7981 GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT 
     8041 CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG TTCCGATTTA GTGCTTTACG GCACCTCGAC 
     8101 CCCAAAAAAC TTGATTAGGG TGATGGTTCA CGTAGTGGGC CATCGCCCTG ATAGACGGTT 
     8161 TTTCGCCCTT TGACGTTGGA GTCCACGTTC TTTAATAGTG GACTCTTGTT CCAAACTGGA 
     8221 ACAACACTCA ACCCTATCTC GGTCTATTCT TTTGATTTAT AAGGGATTTT GCCGATTTCG 
     8281 GCCTATTGGT TAAAAAATGA GCTGATTTAA CAAAAATTTA ACGCGAATTT T
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