LOCUS       RR214341                7902 bp    DNA     circular  SYN 
DEFINITION  Prpf4b (Myc-DDK-tagged ORF) - Rat PRP4 pre-mRNA processing factor 4 homolog B (yeast) (Prpf4b), (10 ug)
ACCESSION   NM_001011923
VERSION     NM_001011923.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4059..4150 
                     /label="Myc-DDK"  
     misc_feature    4180..4765 
                     /label="PolyA signal"  
     misc_feature    4237..4256 
                     /label="Restriction Enzyme"  
     misc_feature    5041..5713 
                     /label="ColE1"  
     misc_feature    6143..6937 
                     /label="Kan/Neo"  
     misc_feature    6972..7274 
                     /label="SV40 ori"  
     misc_feature    7360..7837 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4041..4047 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4041 
                     /label="Prpf4b"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCCGCCACG GAGCCCCCGT CGCTGCGGGA GCAACCTGAG ATGGACGATG 
     1081 CTGATAATTC TGAAAAGAGT GTAAATGAGG AAAATGGAGA AGTATCAGAA GACCAGTCTC 
     1141 AAAATAAACA CAGTCGTCAT AAAAAAAAGA AGCATAAACA CAGAAGTAAA CATAAGAAAC 
     1201 ATAAACATTC CTCAGAAGAA GATAGGGATA AAAAACACAA ACACAAGCAT AAGCATAAGA 
     1261 AACACAAAAG AAAAGAAGTT CTCGATGCCT CTGACAAGGA AGGCCTGTCC CCTGCCAAAA 
     1321 GAACTAAACT TGATGATTTA GCTTTGCTGG AAGACTTGGA AAAACAGAGA GCCTTGATCA 
     1381 AAGCTGAGCT CGATAACGAG TTAATGGAGG GGAAGGTCCA GTCTGGAATG GGGCTCATTC 
     1441 TGCAGGGCTA TGAGTCTGGC TCTGAAGAAG AAGGAGAGAT TCACGAAAAG GCAAGAAATG 
     1501 GAAATAGGTC CAGTACCAGG TCTTCCAGTA CACGGGGAAA ACTTGAAATT ACAGACAACA 
     1561 AAAATAGTGC AAAAAAACGA AGTAAGAGCA GATCCAAAGA ACGAACTAGG CATAGGTCAG 
     1621 ACAAAAGGAA GAGCAAGGGA GCTGTCGAAA TGATGAGAGA AAAAGCAAAC AGGAGCAAGT 
     1681 CTAAGGAGAG GAGAAAATCC AAAAGTCCAT CCAAAAGAAG CAAATCTCAA GACCAAGCAA 
     1741 GAAAATCAAA GTCCCCTACC CTTAGACGGC GGTCCCAAGA GAAGGTGGGG AAGGCTAGAT 
     1801 CTCCTGCTGA CGAGAAGATT AAGAGTGAAG AGAAAGGTAA AATAAAGGAT AGGAAAAAAT 
     1861 CACCAATTGT AAATGAAAGA AGTCGAGATA GATCTAAAAA ATCCAAGTCC CCAGTTGACT 
     1921 TAAGAGATAA ATCCAAAGAC AGAAGGTCGA GGTCCAAAGA GCGGAAGTCA AAACGATCTG 
     1981 AGATTGACAA AGAAAAGAAG CCAATTAAAT CACCTTCAAA AGATGCTTCT TCTGGGAAGG 
     2041 AAAATAGGTC ACCCAGTAGA AGACCTGGCC GTAGTCCTAA AAGAAGAAGT TTGTCTCCAA 
     2101 AACAACGAGA CAAGTCAAGA AGAAGTAGGT CTCCGCTTCT CAATGACAGG AGATCTAAGC 
     2161 AGAGCAAATC ACCTTCTCGA ACTCTCTCTC CAGGCAGAAG GGCCAAGAGC CGATCCTTAG 
     2221 AAAGGAAACG GAGAGAACCA GAGAGGAGAC GGCTTTCTTC TCCGAGAACA CGACCTCGAG 
     2281 ATGATATCCT TGGTAGATGT GAACGATCAA AGGATGCCAG CCCCATTAAC AGGTGGTCTC 
     2341 CAAGCCGAAG AAGAAGTAGG TCTCCCATTA GAAGGAGATC CCGTTCCCCA TTGAGACGCA 
     2401 GCAGGTCTCC TAGAAGAAGA AGCAGGTCTC CTCGAAGAAG AGATAGGAGT CGAAGGAGCA 
     2461 GGTCACGCTT GAGAAGAAGA TCTCGATCAC GGGGTGGTCA TAGGAGAAGG AGCAGAAGCA 
     2521 AAGTAAAGGA AGATAAATTT AAAGGAAGCC TCTCGGAAGG AATGAAAGTT GAGCAAGAGT 
     2581 CTTCGTCTGA TGATAACCTT GAAGATTTTG ATGTGGAGGA AGAAGATGAA GAGGCTGTAA 
     2641 TAGAACAGAG AAGGATACAG AGGCAAGCAA TTGTGCAGAA ATATAAGTAT CTTGCGGAAG 
     2701 ATAGCAATAT ATCTGTGCCA TCTGAGCCAA GCAGCCCCCA GAGCAGCACA AGATCACGGT 
     2761 CCCCTTCGCC AGACGACATC TTGGAGCGGG TAGCTGCTGA CGTAAAAGAA TATGAGCGGG 
     2821 AGAATGTTGA CACATTTGAA GCCTCAGTAA AAGCCAAGCA TAATCTAATG ACAGTTGAAC 
     2881 AGAATAATGG CTCATCTCAG AAGAAGCTTT TGGCACCTGA TATGTTTACA GAATCCGATG 
     2941 ATATGTTTGC TGCTTATTTT GATAGTGCTC GTCTCCGGGC CGCTGGCATT GGGAAAGATT 
     3001 TCAAAGAGAA TCCCAACCTC AGAGATAATT GGACAGATGC AGAAGGCTAT TACCGTGTGA 
     3061 ACATCGGGGA AGTCCTAGAT AAGCGCTATA ATGTATATGG CTACACTGGG CAGGGTGTGT 
     3121 TCAGTAATGT AGTCCGTGCC AGGGATAATG CAAGAGCCAA TCAGGAAGTG GCTGTAAAGA 
     3181 TCATCAGGAA CAATGAACTC ATGCAAAAGA CTGGTTTAAA GGAACTAGAG TTCTTGAAGA 
     3241 AACTGAACGA TGCTGATCCT GATGACAAGT TCCATTGCCT ACGCCTCTTC AGACACTTTT 
     3301 ACCACAAACA GCATCTCTGT CTTGTATTTG AGCCTCTCAG TATGAATTTG CGAGAGGTAT 
     3361 TAAAAAAATA TGGTAAGGAT GTCGGTCTTC ATATTAAGGC TGTACGATCG TACAGTCAGC 
     3421 AGTTGTTCCT GGCATTGAAA CTCCTTAAAA GATGTAATAT CCTACATGCA GATATCAAAC 
     3481 CAGATAATAT TCTGGTTAAT GAATCCAAAA CAATTTTAAA GCTTTGTGAT TTTGGATCGG 
     3541 CGTCACATGT TGCAGACAAT GACATAACGC CTTATCTTGT CAGTAGATTT TATCGTGCTC 
     3601 CGGAGATCAT TATAGGTAAA AGCTATGACT ATGGTATCGA TATGTGGTCT GTAGGTTGTA 
     3661 CCTTATATGA ACTCTATACT GGAAAAATCT TATTTCCTGG CAAAACTAAT AACCACATGT 
     3721 TGAAGCTCGC CATGGACCTT AAAGGAAAAA TGCCAAATAA GATGATCCGG AAAGGTGTAT 
     3781 TCAAAGATCA ACATTTTGAC CAAAATCTGA ACTTCATGTA CATAGAAGTT GATAAAGTAA 
     3841 CAGAGAGGGA GAAAGTTACT GTCATGAGCA CCATTAATCC AACTAAGGAC CTATTGGCTG 
     3901 ACTTGATTGG GTGCCAGCGA CTTCCTGAAG ATCAACGTAA AAAAGTACAC CAGCTAAAGG 
     3961 ACTTGTTGGA CCAGATCCTA ATGTTGGACC CAGCTAAGCG AATTAGCATC AACCAGGCCC 
     4021 TGCAGCATGC ATTCATCCAG GAAAAAATTA CGCGTACGCG GCCGCTCGAG CAGAAACTCA 
     4081 TCTCAGAAGA GGATCTGGCA GCAAATGATA TCCTGGATTA CAAGGATGAC GACGATAAGG 
     4141 TTTAAACGGC CGGCCGCGGT CATAGCTGTT TCCTGAACAG ATCCCGGGTG GCATCCCTGT 
     4201 GACCCCTCCC CAGTGCCTCT CCTGGCCCTG GAAGTTGCCA CTCCAGTGCC CACCAGCCTT 
     4261 GTCCTAATAA AATTAAGTTG CATCATTTTG TCTGACTAGG TGTCCTTCTA TAATATTATG 
     4321 GGGTGGAGGG GGGTGGTATG GAGCAAGGGG CAAGTTGGGA AGACAACCTG TAGGGCCTGC 
     4381 GGGGTCTATT GGGAACCAAG CTGGAGTGCA GTGGCACAAT CTTGGCTCAC TGCAATCTCC 
     4441 GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA 
     4501 TGCATGACCA GGCTCAGCTA ATTTTTGTTT TTTTGGTAGA GACGGGGTTT CACCATATTG 
     4561 GCCAGGCTGG TCTCCAACTC CTAATCTCAG GTGATCTACC CACCTTGGCC TCCCAAATTG 
     4621 CTGGGATTAC AGGCGTGAAC CACTGCTCCC TTCCCTGTCC TTCTGATTTT AAAATAACTA 
     4681 TACCAGCAGG AGGACGTCCA GACACAGCAT AGGCTACCTG GCCATGCCCA ACCGGTGGGA 
     4741 CATTTGAGTT GCTTGCTTGG CACTGTCCTC TCATGCGTTG GGTCCACTCA GTAGATGCCT 
     4801 GTTGAATTGG GTACGCGGCC AGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG 
     4861 GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA 
     4921 GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA 
     4981 CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG 
     5041 ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT 
     5101 TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG 
     5161 CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC 
     5221 CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT 
     5281 AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA 
     5341 TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC 
     5401 AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC 
     5461 TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT 
     5521 TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC 
     5581 TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT 
     5641 CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA 
     5701 ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC GACGTTGGCT GCGAGCCCTG GGCCTTCACC 
     5761 CGAACTTGGG GGGTGGGGTG GGGAAAAGGA AGAAACGCGG GCGTATTGGC CCCAATGGGG 
     5821 TCTCGGTGGG GTATCGACAG AGTGCCAGCC CTGGGACCGA ACCCCGCGTT TATGAACAAA 
     5881 CGACCCAACA CCGTGCGTTT TATTCTGTCT TTTTATTGCC GTCATAGCGC GGGTTCCTTC 
     5941 CGGTATTGTC TCCTTCCGTG TTTCAGTTAG CCTCCCCCTA GGGTGGGCGA AGAACTCCAG 
     6001 CATGAGATCC CCGCGCTGGA GGATCATCCA GCCGGCGTCC CGGAAAACGA TTCCGAAGCC 
     6061 CAACCTTTCA TAGAAGGCGG CGGTGGAATC GAAATCTCGT GATGGCAGGT TGGGCGTCGC 
     6121 TTGGTCGGTC ATTTCGAACC CCAGAGTCCC GCTCAGAAGA ACTCGTCAAG AAGGCGATAG 
     6181 AAGGCGATGC GCTGCGAATC GGGAGCGGCG ATACCGTAAA GCACGAGGAA GCGGTCAGCC 
     6241 CATTCGCCGC CAAGCTCTTC AGCAATATCA CGGGTAGCCA ACGCTATGTC CTGATAGCGA 
     6301 TCCGCCACAC CCAGCCGGCC ACAGTCGATG AATCCAGAAA AGCGGCCATT TTCCACCATG 
     6361 ATATTCGGCA AGCAGGCATC GCCATGGGTC ACGACGAGAT CCTCGCCGTC GGGCATGCTC 
     6421 GCCTTGAGCC TGGCGAACAG TTCGGCTGGC GCGAGCCCCT GATGCTCTTC GTCCAGATCA 
     6481 TCCTGATCGA CAAGACCGGC TTCCATCCGA GTACGTGCTC GCTCGATGCG ATGTTTCGCT 
     6541 TGGTGGTCGA ATGGGCAGGT AGCCGGATCA AGCGTATGCA GCCGCCGCAT TGCATCAGCC 
     6601 ATGATGGATA CTTTCTCGGC AGGAGCAAGG TGAGATGACA GGAGATCCTG CCCCGGCACT 
     6661 TCGCCCAATA GCAGCCAGTC CCTTCCCGCT TCAGTGACAA CGTCGAGCAC AGCTGCGCAA 
     6721 GGAACGCCCG TCGTGGCCAG CCACGATAGC CGCGCTGCCT CGTCTTGCAG TTCATTCAGG 
     6781 GCACCGGACA GGTCGGTCTT GACAAAAAGA ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC 
     6841 ACGGCGGCAT CAGAGCAGCC GATTGTCTGT TGTGCCCAGT CATAGCCGAA TAGCCTCTCC 
     6901 ACCCAAGCGG CCGGAGAACC TGCGTGCAAT CCATCTTGTT CAATCATGCG AAACGATCCT 
     6961 CATCCTGTCT CTTGATCGAT CTTTGCAAAA GCCTAGGCCT CCAAAAAAGC CTCCTCACTA 
     7021 CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC CTCGGCCTCT GCATAAATAA AAAAAATTAG 
     7081 TCAGCCATGG GGCGGAGAAT GGGCGGAACT GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT 
     7141 TAGGGGCGGG ACTATGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     7201 TGGGGAGCCT GGGGACTTTC CACACCTGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     7261 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCCT AACTGACACA CATTCCACAG 
     7321 CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT 
     7381 TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT 
     7441 CCGCGCACAT TTCCCCGAAA AGTGCCACCT GACGCGCCCT GTAGCGGCGC ATTAAGCGCG 
     7501 GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT 
     7561 CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA 
     7621 AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA 
     7681 CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT 
     7741 TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC 
     7801 AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG 
     7861 TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TT
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