LOCUS       RR214307                8286 bp    DNA     circular  SYN 
DEFINITION  Pcdh18 (Myc-DDK-tagged ORF) - Rat protocadherin 18 (Pcdh18), (10 ug)
ACCESSION   NM_001100524
VERSION     NM_001100524.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4443..4534 
                     /label="Myc-DDK"  
     misc_feature    4564..5149 
                     /label="PolyA signal"  
     misc_feature    4621..4640 
                     /label="Restriction Enzyme"  
     misc_feature    5425..6097 
                     /label="ColE1"  
     misc_feature    6527..7321 
                     /label="Kan/Neo"  
     misc_feature    7356..7658 
                     /label="SV40 ori"  
     misc_feature    7744..8221 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4425..4431 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4425 
                     /label="Pcdh18"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCACCAAATA AATACTAAAA TGCACTTTAG ATTCGCACTT TCACTTCTGA 
     1081 TGGCGTTTTT CAGCCACGAT GTCCTGGGTA AGAATCTGAA ATACAGGATT TATGAGGAGC 
     1141 AGAGGGTCGG ATCGGTAATT GCTAGACTAT CAGAAGATGT GGCTGATGTT TTACTGAAGC 
     1201 TACCAAATCC TTCTGCCATT CGTTTTCGAG CCATGCCACG GGGGAATTCT CCTCTCCTGG 
     1261 TCGTAAATGA GAATACCGGA GAAATCAGCA TAGGGGCTAA AATCGACCGC GAGCAACTAT 
     1321 GCCAAAAGAA CTTGAACTGT TCGATAGAGT TTGATGTGCT CACTCTGCCG ACCGAGCATC 
     1381 TGCAGCTGTT CCATATTGAA GTGGACGTGT TGGACATTAA TGACAATTCC CCTCAATTCT 
     1441 CGAGACCCGT CATTCCCATT GAGATATCGG AGAGCGCAGC AGTTGGGACT CGCATCCCTC 
     1501 TGGACAGTGC GTTCGACCCC GATGTTGGGG AAAATTCCCT CCACACCTAC TCGCTCTCTG 
     1561 CTAATGATTA TTTTAATATC GAAGTGCGGA CCAGGACGGA CGGGGCCAAG TATGCTGAAC 
     1621 TCATAGTGGT GAAAGAGTTG GATCGGGAGC TGAAGGCTAG CTATGAGCTG CAGCTCACGG 
     1681 CCTCTGACAT GGGAGTGCCT CAGAGGTCTG GGTCCTCCAT CCTGAAAATA AGCATTTCGG 
     1741 ATTCCAACGA CAACAGCCCG GCCTTTGAGC AGCCCTCTTA CACAATACAA CTCTTAGAAA 
     1801 ACTCCCCAGT TGGAACCTTG CTCCTCGATC TAAATGCCAC TGATCCTGAT GAGGGCGTTA 
     1861 ATGGGAGAAT TGTATATTCT TTCAGCAGCC ATGTGTCTCC CAAAATTATA GAGACTTTTA 
     1921 GGATCGACTC AGAAAGAGGA CACTTGACTC TTTTCAAGCC GGTGGATTAT GAAATCACCA 
     1981 AGTCCTATGA GATAGATGTT CAAGCCCAAG ATCTGGGTCC CAATTCCATT CCTGCTCATT 
     2041 GCAAAATTAT AATTAAGGTC GTGGATGTCA ATGACAATAA GCCTGAAATT AGCATAAACC 
     2101 TCATGTCCCC TGGAAAAGAA GAAGTGTCTT ATGTTTTTGA AGGGGATCCC ATTGATACAT 
     2161 TTGTTGCTAT CGTCAGGGTT CAAGACAAGG ATTCTGGGCT GAATGGGGAA ATAATCTGTA 
     2221 AGCTTCATGG GCATGGGCAC TTTAAACTTC AGAAGACTTA TGAAAACAAC TACTTAATCT 
     2281 TGACCAATGC CACTCTGGAT AGAGAAAAGA GATCTGAGTA CAGTTTGACT GTGATTGCTG 
     2341 AGGACAAGGG AACACCCAAC CTCTCTTCAG TGAGACATTT TACTGTTCAA GTCAATGACA 
     2401 TAAATGACAA TCCACCTCGC TTCCAGAGGA GCCAATATGA ATTTGTCATC TCAGAGAATA 
     2461 ATTCGCCAGG GGCGTACATC ACCACAGTTA CAGCCACCGA TCCAGACCTG GGTGAAAATG 
     2521 GGCATGTGAC ATACACCATT TTGGAGAGTT TTGTCTTGGG AAGTTCCATT ACCACGTATG 
     2581 TAACCATTGA TCCATCCAAT GGCGCCATCT ATGCCCTCAG GATCTTTGAT CATGAGGAAG 
     2641 TGAGTCAGAT CACTTTTGTG GTGGAAGCAA GGGATGGAGG GAGTCAGAAG CAACTCTCAA 
     2701 GCAACACCAC AGTTGTGCTG ACCATCATTG ACGAGAATGA CAATGTCCCT GTGGTTGTAG 
     2761 GGCCTGCAAT GCACAACAAC ACTGCAGACA TCTCCATCCC CAAAGGAGTC GAAAGTGGTT 
     2821 TCCATGTCAC AAGACTAAGG GTAGTGGACA GAGACTCTGG TGCAAATGCT GAATTCACCT 
     2881 GCTCCATAGT AGCTGGTAAT GAGGAAAACA TCTTTATCAT AGATCCAAGG TCATGTGACA 
     2941 TCCATACCAA CGTCAGCATA GACTCCATTT CCTCCACCGA ATGGGCACTT TCAGTTATCA 
     3001 TCCAGGACAA GGGCAATCCT CCTCTGCACA CCAAAGTCCT CCTGAGGTGC ATGGTCTTTG 
     3061 ACTATACAGA ATCTGTGACA AGCACAGCCA TGACTTCTGT CAGTCACGCA TCCTTGGATG 
     3121 TGTCTATGAT CATAATTATT TCCTTAGGGG CAATCTGTGC AGTGTTGCTG GTTATTATGG 
     3181 TACTGTTCGC CACGAGGTGC AATAGAGAAA AGAAAGACAC CAGGTCCTAC AACTGCAGGG 
     3241 TGGCAGAATC CACTTACCAG CATCATCCCA AAAGGCCGTC CAGGCAGATT CACAAAGGAG 
     3301 ACATTACACT GGTGCCCACC ATCAATGGTA CTCTGCCCAT CAGATCTCAC CACAGATCCT 
     3361 CTCCATCTTC ATCTCCAACC TTGGAAAGGG GGCAGATGGG CAGCCGCCAG AGTCACAACA 
     3421 GTCACCAGTC ACTCAACAGT TTGGTGACCA TCTCCTCAAA CCACGTGCCA GAGAATTTTT 
     3481 CATTAGAACT CACCCATGCC ACTCCTGCTG TTGAGCAGGT CTCACAGCTT CTCTCCATGC 
     3541 TCCACCAGGG GCAATATCAG CCACGGCCAA GCTTTCGAGG AAACAAATAT TCCAGGAGCT 
     3601 ATAGATATGC CCTTCAAGAC ATGGATAAAT TTAGCTTGAA AGACAGTGGC CGTGGAGATA 
     3661 GTGAAGCAGG AGACAGTGAT TATGATTTGG GGCGAGATTC TCCAATAGAC AGGCTCCTGG 
     3721 GAGAAGGATT CAGTGACCTC TTTCTCACTG ATGGAAGAAT TCCAGCAGCC ATGAGGCTAT 
     3781 GTACGGAGGA ATGCAGAGTC CTGGGCCACT CTGACCAGTG CTGGATGCCT CCACTGCCCT 
     3841 CGCCATCCTC AGACTACAGA AGCAACATGT TCATCCCCGG AGAAGAGTTC CCGGCTCAAC 
     3901 CTCAACAACA GCATTCTCAT CCGGGCCTTG ATGATGACAG TCAGCCTGCC GAATCTGGGG 
     3961 AGAAAAAAAA GAGCTTTTCC ACCTTTGGGA AGGATTCCCC TAGCGACGAG GACTCCGGGG 
     4021 ACTCTAGCAC ATCATCTCTG CTATCAGAAA TGAACAGTGT GTTCCAACGC CTCCTCCCCG 
     4081 CATCCCTAGA TACCTATTCT GAATGCAGCG AAGTGGACCG GTCCAACTCC CTGGAACGTC 
     4141 GAAAGGGTCC AGCACAGGGC AAAACTGGGG GTTACCCACA AGGGGTTGCA GCCTGGGCAG 
     4201 CCAGCACACA TTTTCAGAAC CCCACCAGCA GCTCTGGGAC CCCTCTGGGG ACTCACTCCA 
     4261 GTGTGCAGCC TTCTTCCAAG TGGTTGCCAG CTATGGAGGA GATCCCTGAA AACTACGAGG 
     4321 AAGATGATTT TGACAATGTG CTGAACCATC TTAGTGATGG GAAACACGAA CTCATGGATG 
     4381 CCAGCGAGCT GGTAGCTGAG ATCAACAAAC TGCTTCAAGA TGTCCGCCAG AGCACGCGTA 
     4441 CGCGGCCGCT CGAGCAGAAA CTCATCTCAG AAGAGGATCT GGCAGCAAAT GATATCCTGG 
     4501 ATTACAAGGA TGACGACGAT AAGGTTTAAA CGGCCGGCCG CGGTCATAGC TGTTTCCTGA 
     4561 ACAGATCCCG GGTGGCATCC CTGTGACCCC TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT 
     4621 GCCACTCCAG TGCCCACCAG CCTTGTCCTA ATAAAATTAA GTTGCATCAT TTTGTCTGAC 
     4681 TAGGTGTCCT TCTATAATAT TATGGGGTGG AGGGGGGTGG TATGGAGCAA GGGGCAAGTT 
     4741 GGGAAGACAA CCTGTAGGGC CTGCGGGGTC TATTGGGAAC CAAGCTGGAG TGCAGTGGCA 
     4801 CAATCTTGGC TCACTGCAAT CTCCGCCTCC TGGGTTCAAG CGATTCTCCT GCCTCAGCCT 
     4861 CCCGAGTTGT TGGGATTCCA GGCATGCATG ACCAGGCTCA GCTAATTTTT GTTTTTTTGG 
     4921 TAGAGACGGG GTTTCACCAT ATTGGCCAGG CTGGTCTCCA ACTCCTAATC TCAGGTGATC 
     4981 TACCCACCTT GGCCTCCCAA ATTGCTGGGA TTACAGGCGT GAACCACTGC TCCCTTCCCT 
     5041 GTCCTTCTGA TTTTAAAATA ACTATACCAG CAGGAGGACG TCCAGACACA GCATAGGCTA 
     5101 CCTGGCCATG CCCAACCGGT GGGACATTTG AGTTGCTTGC TTGGCACTGT CCTCTCATGC 
     5161 GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA TTGGGTACGC GGCCAGCGGC GAGCGGTATC 
     5221 AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA 
     5281 CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT 
     5341 TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG 
     5401 GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG 
     5461 CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG 
     5521 CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC 
     5581 CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA 
     5641 CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG 
     5701 TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC 
     5761 TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC 
     5821 CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG 
     5881 TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT 
     5941 GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT 
     6001 CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA 
     6061 ATCAATCTAA AGTATATATG AGTAACCTGA GGCTATGGCA GGGCCTGCCG CCCCGACGTT 
     6121 GGCTGCGAGC CCTGGGCCTT CACCCGAACT TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC 
     6181 GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG TGGGGTATCG ACAGAGTGCC AGCCCTGGGA 
     6241 CCGAACCCCG CGTTTATGAA CAAACGACCC AACACCGTGC GTTTTATTCT GTCTTTTTAT 
     6301 TGCCGTCATA GCGCGGGTTC CTTCCGGTAT TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC 
     6361 CCTAGGGTGG GCGAAGAACT CCAGCATGAG ATCCCCGCGC TGGAGGATCA TCCAGCCGGC 
     6421 GTCCCGGAAA ACGATTCCGA AGCCCAACCT TTCATAGAAG GCGGCGGTGG AATCGAAATC 
     6481 TCGTGATGGC AGGTTGGGCG TCGCTTGGTC GGTCATTTCG AACCCCAGAG TCCCGCTCAG 
     6541 AAGAACTCGT CAAGAAGGCG ATAGAAGGCG ATGCGCTGCG AATCGGGAGC GGCGATACCG 
     6601 TAAAGCACGA GGAAGCGGTC AGCCCATTCG CCGCCAAGCT CTTCAGCAAT ATCACGGGTA 
     6661 GCCAACGCTA TGTCCTGATA GCGATCCGCC ACACCCAGCC GGCCACAGTC GATGAATCCA 
     6721 GAAAAGCGGC CATTTTCCAC CATGATATTC GGCAAGCAGG CATCGCCATG GGTCACGACG 
     6781 AGATCCTCGC CGTCGGGCAT GCTCGCCTTG AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC 
     6841 CCCTGATGCT CTTCGTCCAG ATCATCCTGA TCGACAAGAC CGGCTTCCAT CCGAGTACGT 
     6901 GCTCGCTCGA TGCGATGTTT CGCTTGGTGG TCGAATGGGC AGGTAGCCGG ATCAAGCGTA 
     6961 TGCAGCCGCC GCATTGCATC AGCCATGATG GATACTTTCT CGGCAGGAGC AAGGTGAGAT 
     7021 GACAGGAGAT CCTGCCCCGG CACTTCGCCC AATAGCAGCC AGTCCCTTCC CGCTTCAGTG 
     7081 ACAACGTCGA GCACAGCTGC GCAAGGAACG CCCGTCGTGG CCAGCCACGA TAGCCGCGCT 
     7141 GCCTCGTCTT GCAGTTCATT CAGGGCACCG GACAGGTCGG TCTTGACAAA AAGAACCGGG 
     7201 CGCCCCTGCG CTGACAGCCG GAACACGGCG GCATCAGAGC AGCCGATTGT CTGTTGTGCC 
     7261 CAGTCATAGC CGAATAGCCT CTCCACCCAA GCGGCCGGAG AACCTGCGTG CAATCCATCT 
     7321 TGTTCAATCA TGCGAAACGA TCCTCATCCT GTCTCTTGAT CGATCTTTGC AAAAGCCTAG 
     7381 GCCTCCAAAA AAGCCTCCTC ACTACTTCTG GAATAGCTCA GAGGCCGAGG CGGCCTCGGC 
     7441 CTCTGCATAA ATAAAAAAAA TTAGTCAGCC ATGGGGCGGA GAATGGGCGG AACTGGGCGG 
     7501 AGTTAGGGGC GGGATGGGCG GAGTTAGGGG CGGGACTATG GTTGCTGACT AATTGAGATG 
     7561 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC TGGTTGCTGA 
     7621 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     7681 CCCTAACTGA CACACATTCC ACAGCTGGTT CTTTCCGCCT CAGGACTCTT CCTTTTTCAA 
     7741 TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT 
     7801 TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC ACCTGACGCG 
     7861 CCCTGTAGCG GCGCATTAAG CGCGGCGGGT GTGGTGGTTA CGCGCAGCGT GACCGCTACA 
     7921 CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC GCTTTCTTCC CTTCCTTTCT CGCCACGTTC 
     7981 GCCGGCTTTC CCCGTCAAGC TCTAAATCGG GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT 
     8041 TTACGGCACC TCGACCCCAA AAAACTTGAT TAGGGTGATG GTTCACGTAG TGGGCCATCG 
     8101 CCCTGATAGA CGGTTTTTCG CCCTTTGACG TTGGAGTCCA CGTTCTTTAA TAGTGGACTC 
     8161 TTGTTCCAAA CTGGAACAAC ACTCAACCCT ATCTCGGTCT ATTCTTTTGA TTTATAAGGG 
     8221 ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA AATGAGCTGA TTTAACAAAA ATTTAACGCG 
     8281 AATTTT
//