LOCUS       RR214189                7935 bp    DNA     circular  SYN 
DEFINITION  Scube1 (Myc-DDK-tagged ORF) - Rat signal peptide, CUB domain, EGF-like 1 (Scube1), (10 ug)
ACCESSION   NM_001134884
VERSION     NM_001134884.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4092..4183 
                     /label="Myc-DDK"  
     misc_feature    4213..4798 
                     /label="PolyA signal"  
     misc_feature    4270..4289 
                     /label="Restriction Enzyme"  
     misc_feature    5074..5746 
                     /label="ColE1"  
     misc_feature    6176..6970 
                     /label="Kan/Neo"  
     misc_feature    7005..7307 
                     /label="SV40 ori"  
     misc_feature    7393..7870 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4074..4080 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4074 
                     /label="Scube1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGGCGCGGCG GCCGTGCGCT GGCACTTGTG CCTGCTGCTC GCTCTGGGAG 
     1081 CGCGAGGGCA GCTGGTGGGG GGCAGCGGGC TGCCAGGAGC CGTGGATGTG GATGAGTGCT 
     1141 CAGAGGGCAC AGATGACTGC CACATAGATG CCATCTGTCA GAACACACCC AAGTCTTACA 
     1201 AATGTCTCTG CAAGCCGGGC TACAAGGGGG AAGGGAAGCA GTGCGAAGAC ATCGATGAGT 
     1261 GTGAGAATGA CTACTACAAC GGGGGCTGTG TCCATGACTG CATCAACATC CCTGGGAACT 
     1321 ACAGGTGCAC CTGCTTTGAC GGCTTCATGC TGGCCCACGA TGGACACAAC TGTCTGGATG 
     1381 TAGATGAGTG CCAAGACAAC AACGGTGGCT GCCAGCAGAT CTGTGTCAAT GCCATGGGCA 
     1441 GCTATGAGTG CCAGTGCCAC AGCGGCTTTT TTCTCAGTGA CAACCAACAC ACCTGCATAC 
     1501 ACCGCTCCAA CGAGGGTATG AACTGCATGA ACAAAGACCA TGGCTGTGCC CACATCTGCC 
     1561 GGGAGACGCC CAAAGGTGGG GTAGCCTGTG ACTGCAGGCC CGGCTTTGAC CTTGCCCAAA 
     1621 ACCAGAAGGA CTGCACACTA ACCTGTAACT ATGGAAACGG AGGCTGTCAG CACAGCTGCG 
     1681 AGGACACGGA CACGGGCCCC ATGTGTGGCT GCCACCAGAA GTACGCCCTA CACGCCGATG 
     1741 GTCGGACGTG CATCGAGAAG GATGAGGCTG CAATTGAGCG CTCTCAGTTC AATGCCACGT 
     1801 CAGTAGCTGA TGTGGACAAG CGTGTGAAAC GGCGGCTCCT CATGGAGACG TGTGCGGTGA 
     1861 ACAACGGAGG CTGTGACCGG ACGTGTAAGG ACACTGCCAC CGGCGTGAGA TGCAGCTGCC 
     1921 CAGTTGGATT CACGCTACAG CCGGATGGAA AGACATGCAA AGACATCAAC GAGTGCTTGA 
     1981 TGAACAACGG AGGCTGCGAC CACTTCTGCC GAAACACTGT GGGCAGCTTT GAATGCGGCT 
     2041 GCCAAAAGGG CCACAAGCTA CTGACGGACG AGCGGACATG CCAAGACATT GACGAGTGTT 
     2101 CCTTTGAGCG GACCTGTGAC CACATCTGCA TCAACTCCCC CGGCAGCTTC CAGTGCCTGT 
     2161 GTCACCGAGG CTACATACTC TACGGGACAA CCCACTGCGG AGACGTGGAT GAGTGCAGCA 
     2221 TGAACAACGG CAGCTGTGAT CAGGGCTGCG TCAACACCAA GGGCAGCTAC GAGTGTGTGT 
     2281 GCCCACCAGG GAGGAGGCTG CACTGGAATC GGAAGGACTG TGTGGAGATG AGCGGGTGCC 
     2341 TGTCTCGGTC CAAGGCCTCT GCCCAGGCCC AGCTGTCCTG TGGCAAGGTG GGTGGAGTGG 
     2401 AAAACTGCTT CCTGTCCTGC CTGGGCCAGA GTCTCTTCAT GCCAGACTCA GAAACCAGCT 
     2461 ACATCCTGAG CTGTGGTGTT CCAGGTCTCC AGGGCAAGGC ACCACCGAAG CGCAATGGCA 
     2521 CCAGCTCCAG TGTGGGGCCC GGCTGCTCAG ATGCCCCCAC CACCCCCATC AGACAGAAGG 
     2581 CCCGCTTCAA GATCAGAGAT GCCAAGTGCC ACCTGCGGCC CCGCAGCCAG GAGCGAGCGA 
     2641 AGGATACCCT GAGGCATCCC CTTTTAGACA ACTGCCACGT GACTTTCGTG ACCCTCAAGT 
     2701 GCGACTCCTC CAAGAAGAGG CGTCGAGGCC GCAAGTCCCC CTCCAAGGAG GTGTCCCACA 
     2761 TCACGGCAGA GTTTGAGGTA GAGATGAAGG TGGAAGAGGC CTCAGGCTCC TGCGAAGCAG 
     2821 ACTGCATGCG GAAGCGTGCC GAGCAGAGCC TGCAGGCTGC CATCAAGACT CTGCGGAAGT 
     2881 CCATCGGCCG GCACCAGTTC TACGTCCAGG TCTCCGGCAC TGAGTACGAG GTGGCCCAGC 
     2941 GGCCAGCCAA GGCACCGGAG GGGACCGGGA CATGTGGCAT AGGCCAAATA CTACAGGATG 
     3001 GCAAGTGTGT ACCATGTGCT CCTGGCACCT ACTTCAGTAG TGAGCCAGGC CAATGCGTGC 
     3061 CGTGTGTGTC GGGAACCTAC CAGGACATGG AAGGCCAGCT CAGCTGCACG CCGTGCCCCA 
     3121 GCAGCGAAGG GCTGGGTCTG GCTGGTGCCC GCAACGTATC CGAATGTGGA GGCCAGTGTT 
     3181 CTCCAGGCTT CTTCTCAGAG GATGGGTTCA AGCCTTGTCA GGCCTGCCCT GTGGGCACAT 
     3241 ACCAGCCTGA GCCTGGGCGT ACCGGCTGCT TCCCCTGTGG AGGTGGGCTG CTCACCAAGC 
     3301 AGACGGGTAC TGCCTCTTTC CAGGAATGTG AGGCCAAAGT ACACTGCTCT CCCGGCCACC 
     3361 ACTACAATAC CACTACCCAC CGGTGCATCC GATGTCCTGT GGGTACCTAT CAACCCGAGT 
     3421 TTGGCCAGAA CCATTGCATC TCCTGTCCGG GCAACACCAG CACCGATTTT GATGGTTCCA 
     3481 CCAATGTCAC ACACTGCAAA AACCAGCACT GCGGTGGCGA GCTTGGTGAC TACACAGGCT 
     3541 ATATCGAGTC CCCCAACTAC CCTGGAGACT ACCCAGCCAA TGCGGAGTGT GTGTGGCACA 
     3601 TTGCGCCTCC CCCCAAGCGC AGGATCCTCA TTGTGGTCCC TGAGATCTTC CTGCCCATCG 
     3661 AGGATGAGTG TGGCGATGTT CTGGTCATGA GGAAGAGCGC CTCGCCCACG TCAGTCACCA 
     3721 CCTATGAGAC GTGCCAGACC TACGAGCGGC CTATCGCCTT CACCTCCCGC TCCCGCAAAC 
     3781 TCTGGATCCA GTTCAAATCC AACGAGGCCA ACAGTGGCAA AGGGTTTCAA GTGCCCTACG 
     3841 TCACCTATGA CGAGGACTAC CAGCAGCTAA TAGAAGACAT CGTGCGCGAT GGGCGCTTGT 
     3901 ATGCCTCGGA GAACCATCAG GAGATTTTGA AAGACAAGAA GCTGATCAAG GCCCTCTTTG 
     3961 ATGTGTTGGC ACACCCACAA AACTACTTCA AATACACAGC CCAGGAGTCA AAGGAGATGT 
     4021 TCCCCCGGTC CTTTATCAAA CTTCTGCGTT CCAAAGTGTC TCGGTTCCTG CGGCCCTACA 
     4081 AAACGCGTAC GCGGCCGCTC GAGCAGAAAC TCATCTCAGA AGAGGATCTG GCAGCAAATG 
     4141 ATATCCTGGA TTACAAGGAT GACGACGATA AGGTTTAAAC GGCCGGCCGC GGTCATAGCT 
     4201 GTTTCCTGAA CAGATCCCGG GTGGCATCCC TGTGACCCCT CCCCAGTGCC TCTCCTGGCC 
     4261 CTGGAAGTTG CCACTCCAGT GCCCACCAGC CTTGTCCTAA TAAAATTAAG TTGCATCATT 
     4321 TTGTCTGACT AGGTGTCCTT CTATAATATT ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG 
     4381 GGGCAAGTTG GGAAGACAAC CTGTAGGGCC TGCGGGGTCT ATTGGGAACC AAGCTGGAGT 
     4441 GCAGTGGCAC AATCTTGGCT CACTGCAATC TCCGCCTCCT GGGTTCAAGC GATTCTCCTG 
     4501 CCTCAGCCTC CCGAGTTGTT GGGATTCCAG GCATGCATGA CCAGGCTCAG CTAATTTTTG 
     4561 TTTTTTTGGT AGAGACGGGG TTTCACCATA TTGGCCAGGC TGGTCTCCAA CTCCTAATCT 
     4621 CAGGTGATCT ACCCACCTTG GCCTCCCAAA TTGCTGGGAT TACAGGCGTG AACCACTGCT 
     4681 CCCTTCCCTG TCCTTCTGAT TTTAAAATAA CTATACCAGC AGGAGGACGT CCAGACACAG 
     4741 CATAGGCTAC CTGGCCATGC CCAACCGGTG GGACATTTGA GTTGCTTGCT TGGCACTGTC 
     4801 CTCTCATGCG TTGGGTCCAC TCAGTAGATG CCTGTTGAAT TGGGTACGCG GCCAGCGGCG 
     4861 AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG GGGATAACGC 
     4921 AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT 
     4981 GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG 
     5041 TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC 
     5101 CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC 
     5161 TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT 
     5221 CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT 
     5281 ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC 
     5341 AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA 
     5401 GTGGTGGCCT AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA 
     5461 GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG 
     5521 TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA 
     5581 AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG 
     5641 GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG 
     5701 AAGTTTTAAA TCAATCTAAA GTATATATGA GTAACCTGAG GCTATGGCAG GGCCTGCCGC 
     5761 CCCGACGTTG GCTGCGAGCC CTGGGCCTTC ACCCGAACTT GGGGGGTGGG GTGGGGAAAA 
     5821 GGAAGAAACG CGGGCGTATT GGCCCCAATG GGGTCTCGGT GGGGTATCGA CAGAGTGCCA 
     5881 GCCCTGGGAC CGAACCCCGC GTTTATGAAC AAACGACCCA ACACCGTGCG TTTTATTCTG 
     5941 TCTTTTTATT GCCGTCATAG CGCGGGTTCC TTCCGGTATT GTCTCCTTCC GTGTTTCAGT 
     6001 TAGCCTCCCC CTAGGGTGGG CGAAGAACTC CAGCATGAGA TCCCCGCGCT GGAGGATCAT 
     6061 CCAGCCGGCG TCCCGGAAAA CGATTCCGAA GCCCAACCTT TCATAGAAGG CGGCGGTGGA 
     6121 ATCGAAATCT CGTGATGGCA GGTTGGGCGT CGCTTGGTCG GTCATTTCGA ACCCCAGAGT 
     6181 CCCGCTCAGA AGAACTCGTC AAGAAGGCGA TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG 
     6241 GCGATACCGT AAAGCACGAG GAAGCGGTCA GCCCATTCGC CGCCAAGCTC TTCAGCAATA 
     6301 TCACGGGTAG CCAACGCTAT GTCCTGATAG CGATCCGCCA CACCCAGCCG GCCACAGTCG 
     6361 ATGAATCCAG AAAAGCGGCC ATTTTCCACC ATGATATTCG GCAAGCAGGC ATCGCCATGG 
     6421 GTCACGACGA GATCCTCGCC GTCGGGCATG CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT 
     6481 GGCGCGAGCC CCTGATGCTC TTCGTCCAGA TCATCCTGAT CGACAAGACC GGCTTCCATC 
     6541 CGAGTACGTG CTCGCTCGAT GCGATGTTTC GCTTGGTGGT CGAATGGGCA GGTAGCCGGA 
     6601 TCAAGCGTAT GCAGCCGCCG CATTGCATCA GCCATGATGG ATACTTTCTC GGCAGGAGCA 
     6661 AGGTGAGATG ACAGGAGATC CTGCCCCGGC ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC 
     6721 GCTTCAGTGA CAACGTCGAG CACAGCTGCG CAAGGAACGC CCGTCGTGGC CAGCCACGAT 
     6781 AGCCGCGCTG CCTCGTCTTG CAGTTCATTC AGGGCACCGG ACAGGTCGGT CTTGACAAAA 
     6841 AGAACCGGGC GCCCCTGCGC TGACAGCCGG AACACGGCGG CATCAGAGCA GCCGATTGTC 
     6901 TGTTGTGCCC AGTCATAGCC GAATAGCCTC TCCACCCAAG CGGCCGGAGA ACCTGCGTGC 
     6961 AATCCATCTT GTTCAATCAT GCGAAACGAT CCTCATCCTG TCTCTTGATC GATCTTTGCA 
     7021 AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA CTACTTCTGG AATAGCTCAG AGGCCGAGGC 
     7081 GGCCTCGGCC TCTGCATAAA TAAAAAAAAT TAGTCAGCCA TGGGGCGGAG AATGGGCGGA 
     7141 ACTGGGCGGA GTTAGGGGCG GGATGGGCGG AGTTAGGGGC GGGACTATGG TTGCTGACTA 
     7201 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCT 
     7261 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     7321 CTTTCCACAC CCTAACTGAC ACACATTCCA CAGCTGGTTC TTTCCGCCTC AGGACTCTTC 
     7381 CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT 
     7441 GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA 
     7501 CCTGACGCGC CCTGTAGCGG CGCATTAAGC GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG 
     7561 ACCGCTACAC TTGCCAGCGC CCTAGCGCCC GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC 
     7621 GCCACGTTCG CCGGCTTTCC CCGTCAAGCT CTAAATCGGG GGCTCCCTTT AGGGTTCCGA 
     7681 TTTAGTGCTT TACGGCACCT CGACCCCAAA AAACTTGATT AGGGTGATGG TTCACGTAGT 
     7741 GGGCCATCGC CCTGATAGAC GGTTTTTCGC CCTTTGACGT TGGAGTCCAC GTTCTTTAAT 
     7801 AGTGGACTCT TGTTCCAAAC TGGAACAACA CTCAACCCTA TCTCGGTCTA TTCTTTTGAT 
     7861 TTATAAGGGA TTTTGCCGAT TTCGGCCTAT TGGTTAAAAA ATGAGCTGAT TTAACAAAAA 
     7921 TTTAACGCGA ATTTT
//