LOCUS       RR214116                7506 bp    DNA     circular  SYN 
DEFINITION  Eln (Myc-DDK-tagged ORF) - Rat elastin (Eln), (10 ug)
ACCESSION   NM_012722
VERSION     NM_012722.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3663..3754 
                     /label="Myc-DDK"  
     misc_feature    3784..4369 
                     /label="PolyA signal"  
     misc_feature    3841..3860 
                     /label="Restriction Enzyme"  
     misc_feature    4645..5317 
                     /label="ColE1"  
     misc_feature    5747..6541 
                     /label="Kan/Neo"  
     misc_feature    6576..6878 
                     /label="SV40 ori"  
     misc_feature    6964..7441 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3645..3651 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3645 
                     /label="Eln"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGGGTCTG ACAGCGGCAG TCCCGCAGCC TGGCGTCTTG CTGATCCTCT 
     1081 TGCTCAACCT CCTCCATCCC GCGCAGCCTG GAGGGGTTCC AGGAGCTGTG CCTGGTGGAG 
     1141 TTCCTGGCGG ACTTCCTGGT GGAGTTCCCG GTGGAGTCTA TTATCCAGGA GCTGGTATCG 
     1201 GAGGAGGCCT GGGAGGAGGA GCTCTGGGAC CTGGAGGAAA ACCGCCTAAG CCAGGTGCCG 
     1261 GACTTCTGGG AGCGTTTGGA GCAGGTCCTG GAGGACTTGG AGGTGCTGGC CCCGGGGCAG 
     1321 GTCTCTCCTA TGCTTCAAGG CCAGGTGGTG TTCTGGTGCC TGGGGGAGGA GCAGGGGCTG 
     1381 CAGCAGCTTA TAAAGCCGCG GCCAAAGCTG GGGCTGGGCT TGGAGGCATT GGCGGAGTTC 
     1441 CAGGGGGTGT TGGAGTTGGT GGAGTTCCTG GGGCTGTTGG AGTTGGCGGA GTTCCTGGGG 
     1501 CTGTCGGTGG TATTGGTGGC ATCGGTGGCT TAGGAGTCTC AACAGGTGCT GTGGTGCCTC 
     1561 AACTTGGAGC TGGAGTCGGA GCCGGAGGAA AGCCTGGGAA AGTTCCTGGT GTCGGTCTTC 
     1621 CAGGTGTATA CCCAGGTGGA GTGCTCCCAG GAACAGGAGC TCGGTTCCCT GGTGTGGGAG 
     1681 TGCTCCCTGG AGTTCCCACT GGCACAGGAG TCAAGGCCAA GGTTCCGGGT GGAGGAGGTG 
     1741 GTGCTTTTTC TGGAATCCCA GGGGTCGGGC CCTTTGGGGG TCAGCAGCCT GGTGTCCCAC 
     1801 TGGGTTATCC CATCAAAGCG CCAAAGCTGC CAGGTGGCTA TGGACTGCCC TATACCAATG 
     1861 GGAAACTGCC CTATGGAGTG GCTGGTGCAG GGGGCAAAGC TGGCTACCCA ACAGGGACAG 
     1921 GGGTCGGGTC CCAGGCAGCA GTGGCAGCAG CTAAAGCAGC GAAGTATGGT GCTGGAGGAG 
     1981 GTGGAGTCCT CCCTGGTGTT GGAGGGGGTG GCATTCCTGG TGGTGCTGGC GCAATTCCTG 
     2041 GGATTGGAGG CATTACAGGC GCTGGAACTC CCGCAGCAGC TGCTGCTGCA AAGGCTGCCG 
     2101 CTAAGGCTGC TAAGTATGGA GCTGCTGGAG GCTTAGTGCC TGGTGGACCA GGAGTTAGGG 
     2161 TCCCAGGTGC TGGAATCCCA GGTGTTGGGA TCCCAGGTGT CGGTGGCATT CCAGGTGTTG 
     2221 GTGGCATTCC AGGTGTTGGT GGCATCCCAG GTGTTGGGGG CCCTGGTATC GGAGGTCCAG 
     2281 GCATTGTGGG TGGACCAGGG GCTGTGTCAC CAGCTGCAGC TGCCAAAGCT GCTGCCAAAG 
     2341 CTGCCAAGTA TGGAGCCAGA GGTGGAGTTG GCATCCCGAC ATACGGGGTT GGTGCTGGTG 
     2401 GCTTTCCTGG CTATGGTGTT GGAGCTGGAG CTGGACTTGG AGGTGCAAGC CAAGCTGCTG 
     2461 CTGCCGCTGC CGCTGCCAAA GCTGCCAAGT ACGGTGCCGG AGGAGCTGGA ACCCTGGGAG 
     2521 GCCTGGTGCC AGGTGCAGTA CCAGGTGCAC TGCCAGGTGC AGTACCAGGT GCACTGCCAG 
     2581 GTGCAGTACC AGGTGCACTG CCAGGTGCAG TGCCAGGTGT GCCGGGCACT GGTGGAGTGC 
     2641 CAGGAGCAGG TACCCCTGCG GCTGCAGCTG CTGCCGCCGC TGCCAAGGCA GCCGCCAAGG 
     2701 CAGGCCAGTA TGGTTTGGGC CCTGGTGTTG GTGGCGTTCC CGGTGGAGTT GGTGTTGGTG 
     2761 GGCTTCCCGG TGGAGTTGGC CCTGGTGGTG TTACTGGTAT TGGAACTGGT CCTGGCACCG 
     2821 GCCTTGTACC TGGTGATCTT GGAGGAGCAG GGACACCAGC TGCTGCCAAA TCTGCTGCTA 
     2881 AGGCGGCTGC CAAAGCCCAG TACAGGGCTG CTGCTGGGCT TGGGGCCGGT GTCCCTGGAC 
     2941 TTGGGGTTGG TGCTGGTGTC CCCGGATTTG GGGCTGGTGC TGGTGGATTT GGGGCTGGTG 
     3001 CTGGTGTCCC TGGATTTGGA GCTGGAGCAG TTCCTGGATC CCTGGCTGCA TCCAAAGCTG 
     3061 CTAAATACGG AGCAGCAGGT GGCCTTGGAG GGCCTGGAGG ACTTGGTGGG CCTGGAGGAC 
     3121 TTGGTGGGCC CGGAGGACTT GGTGGGCCTG GAGGACTCGG TGGGCCTGGA GGACTCGGTG 
     3181 GTGTTCCTGG TGGAGTAGCA GGAGGTGCAC CTGCTGCTGC TGCTGCTGCC AAAGCTGCCG 
     3241 CTAAGGCTGC CCAGTACGGT CTTGGTGGAG CTGGAGGACT CGGGGCCGGT GGACTGGGAG 
     3301 CCGGAGGACT GGGGGCCGGT GGACTGGGAG CCGGAGGACT GGGAGCTGGT GGACTGGGGG 
     3361 CTGGGGGAGT GATCCCTGGT GCTGTAGGCC TTGGAGGTGT GTCCCCTGCT GCAGCTGCTA 
     3421 AGGCTGCCAA ATATGGTGCT GCTGGCCTTG GAGGTGTCCT AGGAGCCAGG CCATTCCCAG 
     3481 GCGGAGGAGT GGCAGCAAGA CCTGGGTTTG GACTTTCTCC TATCTACCCA GGTGGTGGTG 
     3541 CTGGGGGCCT GGGAGTTGGT GGAAAACCCC CGAAGCCCTA TGGAGGAGCC CTTGGAGCCC 
     3601 TGGGATATCA AGGTGGGGGC TGCTTTGGGA AATCTTGTGG CCGGAAGAGA AAGACGCGTA 
     3661 CGCGGCCGCT CGAGCAGAAA CTCATCTCAG AAGAGGATCT GGCAGCAAAT GATATCCTGG 
     3721 ATTACAAGGA TGACGACGAT AAGGTTTAAA CGGCCGGCCG CGGTCATAGC TGTTTCCTGA 
     3781 ACAGATCCCG GGTGGCATCC CTGTGACCCC TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT 
     3841 GCCACTCCAG TGCCCACCAG CCTTGTCCTA ATAAAATTAA GTTGCATCAT TTTGTCTGAC 
     3901 TAGGTGTCCT TCTATAATAT TATGGGGTGG AGGGGGGTGG TATGGAGCAA GGGGCAAGTT 
     3961 GGGAAGACAA CCTGTAGGGC CTGCGGGGTC TATTGGGAAC CAAGCTGGAG TGCAGTGGCA 
     4021 CAATCTTGGC TCACTGCAAT CTCCGCCTCC TGGGTTCAAG CGATTCTCCT GCCTCAGCCT 
     4081 CCCGAGTTGT TGGGATTCCA GGCATGCATG ACCAGGCTCA GCTAATTTTT GTTTTTTTGG 
     4141 TAGAGACGGG GTTTCACCAT ATTGGCCAGG CTGGTCTCCA ACTCCTAATC TCAGGTGATC 
     4201 TACCCACCTT GGCCTCCCAA ATTGCTGGGA TTACAGGCGT GAACCACTGC TCCCTTCCCT 
     4261 GTCCTTCTGA TTTTAAAATA ACTATACCAG CAGGAGGACG TCCAGACACA GCATAGGCTA 
     4321 CCTGGCCATG CCCAACCGGT GGGACATTTG AGTTGCTTGC TTGGCACTGT CCTCTCATGC 
     4381 GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA TTGGGTACGC GGCCAGCGGC GAGCGGTATC 
     4441 AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA 
     4501 CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT 
     4561 TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG 
     4621 GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG 
     4681 CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG 
     4741 CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC 
     4801 CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA 
     4861 CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG 
     4921 TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC 
     4981 TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC 
     5041 CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG 
     5101 TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT 
     5161 GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT 
     5221 CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA 
     5281 ATCAATCTAA AGTATATATG AGTAACCTGA GGCTATGGCA GGGCCTGCCG CCCCGACGTT 
     5341 GGCTGCGAGC CCTGGGCCTT CACCCGAACT TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC 
     5401 GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG TGGGGTATCG ACAGAGTGCC AGCCCTGGGA 
     5461 CCGAACCCCG CGTTTATGAA CAAACGACCC AACACCGTGC GTTTTATTCT GTCTTTTTAT 
     5521 TGCCGTCATA GCGCGGGTTC CTTCCGGTAT TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC 
     5581 CCTAGGGTGG GCGAAGAACT CCAGCATGAG ATCCCCGCGC TGGAGGATCA TCCAGCCGGC 
     5641 GTCCCGGAAA ACGATTCCGA AGCCCAACCT TTCATAGAAG GCGGCGGTGG AATCGAAATC 
     5701 TCGTGATGGC AGGTTGGGCG TCGCTTGGTC GGTCATTTCG AACCCCAGAG TCCCGCTCAG 
     5761 AAGAACTCGT CAAGAAGGCG ATAGAAGGCG ATGCGCTGCG AATCGGGAGC GGCGATACCG 
     5821 TAAAGCACGA GGAAGCGGTC AGCCCATTCG CCGCCAAGCT CTTCAGCAAT ATCACGGGTA 
     5881 GCCAACGCTA TGTCCTGATA GCGATCCGCC ACACCCAGCC GGCCACAGTC GATGAATCCA 
     5941 GAAAAGCGGC CATTTTCCAC CATGATATTC GGCAAGCAGG CATCGCCATG GGTCACGACG 
     6001 AGATCCTCGC CGTCGGGCAT GCTCGCCTTG AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC 
     6061 CCCTGATGCT CTTCGTCCAG ATCATCCTGA TCGACAAGAC CGGCTTCCAT CCGAGTACGT 
     6121 GCTCGCTCGA TGCGATGTTT CGCTTGGTGG TCGAATGGGC AGGTAGCCGG ATCAAGCGTA 
     6181 TGCAGCCGCC GCATTGCATC AGCCATGATG GATACTTTCT CGGCAGGAGC AAGGTGAGAT 
     6241 GACAGGAGAT CCTGCCCCGG CACTTCGCCC AATAGCAGCC AGTCCCTTCC CGCTTCAGTG 
     6301 ACAACGTCGA GCACAGCTGC GCAAGGAACG CCCGTCGTGG CCAGCCACGA TAGCCGCGCT 
     6361 GCCTCGTCTT GCAGTTCATT CAGGGCACCG GACAGGTCGG TCTTGACAAA AAGAACCGGG 
     6421 CGCCCCTGCG CTGACAGCCG GAACACGGCG GCATCAGAGC AGCCGATTGT CTGTTGTGCC 
     6481 CAGTCATAGC CGAATAGCCT CTCCACCCAA GCGGCCGGAG AACCTGCGTG CAATCCATCT 
     6541 TGTTCAATCA TGCGAAACGA TCCTCATCCT GTCTCTTGAT CGATCTTTGC AAAAGCCTAG 
     6601 GCCTCCAAAA AAGCCTCCTC ACTACTTCTG GAATAGCTCA GAGGCCGAGG CGGCCTCGGC 
     6661 CTCTGCATAA ATAAAAAAAA TTAGTCAGCC ATGGGGCGGA GAATGGGCGG AACTGGGCGG 
     6721 AGTTAGGGGC GGGATGGGCG GAGTTAGGGG CGGGACTATG GTTGCTGACT AATTGAGATG 
     6781 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC TGGTTGCTGA 
     6841 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     6901 CCCTAACTGA CACACATTCC ACAGCTGGTT CTTTCCGCCT CAGGACTCTT CCTTTTTCAA 
     6961 TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT 
     7021 TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC ACCTGACGCG 
     7081 CCCTGTAGCG GCGCATTAAG CGCGGCGGGT GTGGTGGTTA CGCGCAGCGT GACCGCTACA 
     7141 CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC GCTTTCTTCC CTTCCTTTCT CGCCACGTTC 
     7201 GCCGGCTTTC CCCGTCAAGC TCTAAATCGG GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT 
     7261 TTACGGCACC TCGACCCCAA AAAACTTGAT TAGGGTGATG GTTCACGTAG TGGGCCATCG 
     7321 CCCTGATAGA CGGTTTTTCG CCCTTTGACG TTGGAGTCCA CGTTCTTTAA TAGTGGACTC 
     7381 TTGTTCCAAA CTGGAACAAC ACTCAACCCT ATCTCGGTCT ATTCTTTTGA TTTATAAGGG 
     7441 ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA AATGAGCTGA TTTAACAAAA ATTTAACGCG 
     7501 AATTTT
//