LOCUS       RR214025                8763 bp    DNA     circular  SYN 
DEFINITION  Rapgef2 (Myc-DDK-tagged ORF) - Rat Rap guanine nucleotide exchange factor (GEF) 2 (Rapgef2), (10 ug)
ACCESSION   NM_001107684
VERSION     NM_001107684.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4920..5011 
                     /label="Myc-DDK"  
     misc_feature    5041..5626 
                     /label="PolyA signal"  
     misc_feature    5098..5117 
                     /label="Restriction Enzyme"  
     misc_feature    5902..6574 
                     /label="ColE1"  
     misc_feature    7004..7798 
                     /label="Kan/Neo"  
     misc_feature    7833..8135 
                     /label="SV40 ori"  
     misc_feature    8221..8698 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4902..4908 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4902 
                     /label="Rapgef2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGGAAACAGC TTTGGTGTTT CGCCCACCAT GGACAAAGAG TACATGAAAG 
     1081 GAGTCATGAG GACAAAGGTG GATGACTGCC AGTTTGTCTG CATTGCCCAG CAAGATTACT 
     1141 GCCGTATTTT GAACCAAGTA GAAAAGAACA TGCAAAAAGT TGAGGAAGAA GGAGAGATTG 
     1201 TAATGGTGAA GGAGCACCGA GAACTTGATC GGACTGGAAC AAGGAAGGGC CACATCGTCA 
     1261 TCAAGGGCAC CTCAGAGAGG CTAACCATGC ATTTGGTGGA AGAGCATTCT GTAGTGGACC 
     1321 CAACATTCAT AGAAGACTTC CTGTTGACAT ACCGGACTTT CCTTTCCAGC CCAATGGAAG 
     1381 TGGGCAAGAA GTTATTGGAG TGGTTCAATG ACCCGAGCCT CAGGGATAAG GTGACACGGG 
     1441 TAGTATTACT GTGGGTGAAT AATCACTTCA ATGACTTTGA AGGAGATCCT GCAATGACTC 
     1501 GATTTCTAGA AGAATTTGAA AATAATCTGG AAAGAGAGAA AATGGGTGGA CATCTAAGGC 
     1561 TGCTAAACAT TGCATGTGCA GCGAAAGCCA AACGAAGGTC GATGACGTTA ACAAAGCCGT 
     1621 CCCGGGAGGC GCCTCTGCCC TTCATCCTGC TGGGAGGCTC TGAAAAGGGC TTCGGCATCT 
     1681 TTGTGGACAG TGTTGACTCA TCGAGCAAAG CCACTGAAGC AGGCTTGAAG CGTGGGGATC 
     1741 AGATATTAGA AGTGAATGGT CAAAATTTTG AAAATATCCA ACTTTCAAAG GCAATGGAAA 
     1801 TTCTTAGAAA TAACACACAC CTGTCCATCA CTGTGAAAAC CAACTTATTT GTATTTAAAG 
     1861 AACTTCTGAC GAGATTGTCT GAAGAGAAAA GAAATGGTGC CCCCCACCTT CCTAAAATTG 
     1921 GAGACATCAA GAAGGCCAGT CGCTACTCCA TACCGGATCT TGCTGTAGAT GTAGAACAGG 
     1981 TGATAGGCCT CGAGAAGGTC AACAAGAAAA GCAAAGCCAA CACTGTGGGG GGAAGAAACA 
     2041 AGCTAAAGAA AATACTCGAC AAGACTCGGA TCAGTATCTT ACCTCAGAAA CCATACAATG 
     2101 ATATTGGGAT TGGTCAGTCT CAAGATGATA GCATCGTAGG ACTGAGGCAG ACCAAGCACA 
     2161 TTCCAGCTGC CCTGCCTGTC AGTGGAACGC TGTCTTCCAG TAACCCTGAC TTGCTGCAGT 
     2221 CACACCACCG GATTCTGGAC TTCAGCACTA CTCCTGACTT GCCAGATCAG GTACTGAGAG 
     2281 TTTTCAAGGC TGACCAGCAA AGCAGATACA TCATGATCAG TAAGGACACC ACAGCAAAGG 
     2341 AAGTGGTCAT CCAGGCCATC AGAGAGTTTG CTGTTACTGC CACCCCAGAG CAGTATTCCC 
     2401 TCTGTGAGGT CTCGGTCACG CCAGAGGGAG TTATCAAACA ACGAAGGCTC CCAGATCAAC 
     2461 TTTCCAAGCT TGCTGACAGA ATACAGCTGA GCGGGAGGTA TTATCTGAAA AACAACATGG 
     2521 AAACAGAAAC GCTTTGCTCA GATGAAGATG CCCAGGAGCT GTTGCGAGAG AGCCAAATTT 
     2581 CACTTCTTCA GCTCAGCACT GTGGAGGTCG CCACACAGCT CTCCATGAGG AACTTTGAAC 
     2641 TCTTCCGCAA CATTGAGCCT ACTGAATACA TAGATGATTT ATTTAAACTC AAATCAAAAA 
     2701 CCAGCTGTGC CAACCTAAAG AAATTTGAGG AAGTCATTAA CCAGGAAACA TTTTGGGTGG 
     2761 CTTCTGAAAT TCTCCGAGAG ACAAACCAGC TGAAGAGAAT GAAGATCATT AAGCATTTCA 
     2821 TCAAGATTGC CTTGCACTGT AGGGAATGCA AGAATTTTAA CTCCATGTTT GCAATCATCA 
     2881 GTGGCCTAAA CCTGGCACCA GTGGCAAGAC TACGGACCAC CTGGGAAAAA CTTCCCAATA 
     2941 AATATGAAAA GCTCTTCCAG GACCTACAAG ACCTGTTTGA TCCTTCCAGA AACATGGCTA 
     3001 AGTATCGCAA TGTCCTGAGT GGCCAGAACT TACAGCCTCC TGTCATTCCC CTATTCCCCG 
     3061 TCATCAAGAA GGACCTCACG TTTCTCCATG AGGGAAATGA CTCAAAAGTA GATGGGCTGG 
     3121 TCAACTTTGA GAAGCTGAGG ATGATTGCAA AGGAAATCCG TCATGTTGGC CGAATGGCCT 
     3181 CAGTCAACAT GGACCCTGCC CTTATGTTCA GAACTCGGTC GCTGAGTCAG GGCAGTGCGA 
     3241 ATGCCACAGT GCTGGATGTT GCTCAGACAG GCGGGCATAA AAAGCGGGTA CGCCGTAGTT 
     3301 CCTTCCTCAA TGCCAAAAAG CTGTATGAAG ATGCCCAAAT GGCTCGGAAA GTGAAGCAAT 
     3361 ACCTGTCCAA CCTGGAGCTG GAGATGGACG AGGAGAGTCT TCAGACACTG TCACTTCAGT 
     3421 GTGAACCAGC AACCAGCACA TTGCCCAAGA ACCCTGCAGA CAGGAAGCCT GTCAAGTCCG 
     3481 AGACCTCCCC AGTGGCTCCA CGTGCAGGGC CCCAGCAGAA AGCGCAGCCT CAGCAGCCGT 
     3541 TGGCACCCCC TCAACCATCG CATAAAGTCA GCCAGGGGCT GCAGGTGCCT GCTGTGTCCC 
     3601 TCTACCCCTC ACGAAAGAAG GTGCCAGTGA AGGATCTGCC GCCCTTTGGC ATAAACTCTC 
     3661 CACAAGCTTT AAAGAAAATC CTGTCCTTGT CTGAAGAAGG GAGTTTGGAG CGTCATAGGA 
     3721 AGCAAGCAGA AGACACAATA TCAAACGCAT CCTCACAGCT CTCATCACCC CCCACCTCAC 
     3781 CTCAGAGCTC TCCAAGGAAA GGGTATGCAT TGGCTCTCAG TGGGACTGTG GATAATTTCT 
     3841 CAGATTCTGG TCACAGTGAG ATTTCTTCAC GATCCAGTAT TGTCAGCAAT TCTTCTTTTG 
     3901 ACTCGGTGCC AGTCTCTCTG CATGATGAAC GGCGCCAGAG GCATTCTGTC AGCATTGTGG 
     3961 AGAGCAACCT AGGGGTGGGC AGGATGGAGC GGCGGACCCT GATGGAGCCT GATCAATACA 
     4021 GCCTAGGATC ATATGCCCCA GTGTCTGAAA GTCGAGGCCT GTATGCCGCA GCGACAGTCA 
     4081 TCTCTTCTCC TAGCACAGAG GAGCTTTCCC ACGACCAAGG GGACCGTGCT TCTCTCGATG 
     4141 CTGCTGACAG CGGTCGGGGG AGCTGGACAT CATGCTCAAG TGGTTCTCAT GACAACATAC 
     4201 AGACCATCCA GCACCAGAGA AGCTGGGAAA CACTGCCATT TGGGCACACT CACTTTGACT 
     4261 ATTCAGGGGA TGCGACAGGT ATATGGGCCT CCGGCGGCCA TATGGACCAA ATCATGTTTT 
     4321 CTGATCATAG CACAAAATAT AATAGACAGA ATCAGAGTAG AGAGAGTCTT GAACAAGCCC 
     4381 AATCCCGGGC AAGCTGGGCG TCTTCCACGG GCTACTGGGG AGAAGACTCA GAAGGCGACA 
     4441 CGGGCACTAT CAAGCGAAGG GGTGGGAAGG ATGTCTCCAC TGAGGCAGAG AGCAGCAGCA 
     4501 TGGTGCCTGT GACTACAGAG GAAGCTAAGC CTGTCCCTAT GCCTGCCCAC ATAGCCGTGA 
     4561 CGCCCAGCAC TACCAAGGGA CTCATCGCAC GAAAGGAAGG CAGGTACCGG GAGCCGCCTC 
     4621 CCACACCTCC AGGCTACGTG GGCATTCCCA TTGCCGATTT CCCAGAAGGG CCTTGCCACC 
     4681 CCGCCAGGAA GCCCCCGGAT TACAACGTGG CCCTGCAGCG GTCCCGCATG GTGGCACGGC 
     4741 CCACTGAGGC CCCCGCACCA GGCCAGACGC CGCCGGCAGC CACAGCCAGC CGGCCGGGCA 
     4801 GCAAACCGCA GTGGCACAAG CCCAGCGACG CAGACCCACG CCTCGCGCCC TTCCAGCCGC 
     4861 AGGGCTTCGC CGGAGCGGAG GAGGACGAAG ATGAACAAGT GTCTGCTGTT ACGCGTACGC 
     4921 GGCCGCTCGA GCAGAAACTC ATCTCAGAAG AGGATCTGGC AGCAAATGAT ATCCTGGATT 
     4981 ACAAGGATGA CGACGATAAG GTTTAAACGG CCGGCCGCGG TCATAGCTGT TTCCTGAACA 
     5041 GATCCCGGGT GGCATCCCTG TGACCCCTCC CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC 
     5101 ACTCCAGTGC CCACCAGCCT TGTCCTAATA AAATTAAGTT GCATCATTTT GTCTGACTAG 
     5161 GTGTCCTTCT ATAATATTAT GGGGTGGAGG GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG 
     5221 AAGACAACCT GTAGGGCCTG CGGGGTCTAT TGGGAACCAA GCTGGAGTGC AGTGGCACAA 
     5281 TCTTGGCTCA CTGCAATCTC CGCCTCCTGG GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC 
     5341 GAGTTGTTGG GATTCCAGGC ATGCATGACC AGGCTCAGCT AATTTTTGTT TTTTTGGTAG 
     5401 AGACGGGGTT TCACCATATT GGCCAGGCTG GTCTCCAACT CCTAATCTCA GGTGATCTAC 
     5461 CCACCTTGGC CTCCCAAATT GCTGGGATTA CAGGCGTGAA CCACTGCTCC CTTCCCTGTC 
     5521 CTTCTGATTT TAAAATAACT ATACCAGCAG GAGGACGTCC AGACACAGCA TAGGCTACCT 
     5581 GGCCATGCCC AACCGGTGGG ACATTTGAGT TGCTTGCTTG GCACTGTCCT CTCATGCGTT 
     5641 GGGTCCACTC AGTAGATGCC TGTTGAATTG GGTACGCGGC CAGCGGCGAG CGGTATCAGC 
     5701 TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT 
     5761 GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT 
     5821 CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG 
     5881 AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC 
     5941 TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT 
     6001 GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA 
     6061 GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA 
     6121 TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA 
     6181 CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA 
     6241 CTACGGCTAC ACTAGAAGAA CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT 
     6301 CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT 
     6361 TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT 
     6421 CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT 
     6481 GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC 
     6541 AATCTAAAGT ATATATGAGT AACCTGAGGC TATGGCAGGG CCTGCCGCCC CGACGTTGGC 
     6601 TGCGAGCCCT GGGCCTTCAC CCGAACTTGG GGGGTGGGGT GGGGAAAAGG AAGAAACGCG 
     6661 GGCGTATTGG CCCCAATGGG GTCTCGGTGG GGTATCGACA GAGTGCCAGC CCTGGGACCG 
     6721 AACCCCGCGT TTATGAACAA ACGACCCAAC ACCGTGCGTT TTATTCTGTC TTTTTATTGC 
     6781 CGTCATAGCG CGGGTTCCTT CCGGTATTGT CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT 
     6841 AGGGTGGGCG AAGAACTCCA GCATGAGATC CCCGCGCTGG AGGATCATCC AGCCGGCGTC 
     6901 CCGGAAAACG ATTCCGAAGC CCAACCTTTC ATAGAAGGCG GCGGTGGAAT CGAAATCTCG 
     6961 TGATGGCAGG TTGGGCGTCG CTTGGTCGGT CATTTCGAAC CCCAGAGTCC CGCTCAGAAG 
     7021 AACTCGTCAA GAAGGCGATA GAAGGCGATG CGCTGCGAAT CGGGAGCGGC GATACCGTAA 
     7081 AGCACGAGGA AGCGGTCAGC CCATTCGCCG CCAAGCTCTT CAGCAATATC ACGGGTAGCC 
     7141 AACGCTATGT CCTGATAGCG ATCCGCCACA CCCAGCCGGC CACAGTCGAT GAATCCAGAA 
     7201 AAGCGGCCAT TTTCCACCAT GATATTCGGC AAGCAGGCAT CGCCATGGGT CACGACGAGA 
     7261 TCCTCGCCGT CGGGCATGCT CGCCTTGAGC CTGGCGAACA GTTCGGCTGG CGCGAGCCCC 
     7321 TGATGCTCTT CGTCCAGATC ATCCTGATCG ACAAGACCGG CTTCCATCCG AGTACGTGCT 
     7381 CGCTCGATGC GATGTTTCGC TTGGTGGTCG AATGGGCAGG TAGCCGGATC AAGCGTATGC 
     7441 AGCCGCCGCA TTGCATCAGC CATGATGGAT ACTTTCTCGG CAGGAGCAAG GTGAGATGAC 
     7501 AGGAGATCCT GCCCCGGCAC TTCGCCCAAT AGCAGCCAGT CCCTTCCCGC TTCAGTGACA 
     7561 ACGTCGAGCA CAGCTGCGCA AGGAACGCCC GTCGTGGCCA GCCACGATAG CCGCGCTGCC 
     7621 TCGTCTTGCA GTTCATTCAG GGCACCGGAC AGGTCGGTCT TGACAAAAAG AACCGGGCGC 
     7681 CCCTGCGCTG ACAGCCGGAA CACGGCGGCA TCAGAGCAGC CGATTGTCTG TTGTGCCCAG 
     7741 TCATAGCCGA ATAGCCTCTC CACCCAAGCG GCCGGAGAAC CTGCGTGCAA TCCATCTTGT 
     7801 TCAATCATGC GAAACGATCC TCATCCTGTC TCTTGATCGA TCTTTGCAAA AGCCTAGGCC 
     7861 TCCAAAAAAG CCTCCTCACT ACTTCTGGAA TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC 
     7921 TGCATAAATA AAAAAAATTA GTCAGCCATG GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT 
     7981 TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG GACTATGGTT GCTGACTAAT TGAGATGCAT 
     8041 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCTGG TTGCTGACTA 
     8101 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCC 
     8161 TAACTGACAC ACATTCCACA GCTGGTTCTT TCCGCCTCAG GACTCTTCCT TTTTCAATAT 
     8221 TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG 
     8281 AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC TGACGCGCCC 
     8341 TGTAGCGGCG CATTAAGCGC GGCGGGTGTG GTGGTTACGC GCAGCGTGAC CGCTACACTT 
     8401 GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT TTCTTCCCTT CCTTTCTCGC CACGTTCGCC 
     8461 GGCTTTCCCC GTCAAGCTCT AAATCGGGGG CTCCCTTTAG GGTTCCGATT TAGTGCTTTA 
     8521 CGGCACCTCG ACCCCAAAAA ACTTGATTAG GGTGATGGTT CACGTAGTGG GCCATCGCCC 
     8581 TGATAGACGG TTTTTCGCCC TTTGACGTTG GAGTCCACGT TCTTTAATAG TGGACTCTTG 
     8641 TTCCAAACTG GAACAACACT CAACCCTATC TCGGTCTATT CTTTTGATTT ATAAGGGATT 
     8701 TTGCCGATTT CGGCCTATTG GTTAAAAAAT GAGCTGATTT AACAAAAATT TAACGCGAAT 
     8761 TTT
//