LOCUS       RR214024                8403 bp    DNA     circular  SYN 
DEFINITION  Nfasc (Myc-DDK-tagged ORF) - Rat neurofascin (Nfasc), (10 ug)
ACCESSION   NM_053909
VERSION     NM_053909.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4560..4651 
                     /label="Myc-DDK"  
     misc_feature    4681..5266 
                     /label="PolyA signal"  
     misc_feature    4738..4757 
                     /label="Restriction Enzyme"  
     misc_feature    5542..6214 
                     /label="ColE1"  
     misc_feature    6644..7438 
                     /label="Kan/Neo"  
     misc_feature    7473..7775 
                     /label="SV40 ori"  
     misc_feature    7861..8338 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4542..4548 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4542 
                     /label="Nfasc"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCCAGGCAG CAGGCGCCAC CCTGGGTCCA CGTAGCCCTC ATCCTCTTCC 
     1081 TCCTCAGCCT CGGAGGGGCC ATTGAGATTC CGATGGATCT GACCCAACCC CCAACGATCA 
     1141 CCAAGCAGTC GGTGAAGGAC CACATCGTGG ACCCCCGAGA TAACATCCTG ATTGAATGTG 
     1201 AAGCTAAAGG GAACCCCGCC CCCAGTTTCC ACTGGACTCG CAACAGCAGG TTCTTCAACA 
     1261 TTGCCAAGGA CCCACGGGTG TCCATGAGGA GGAGGTCTGG GACCTTGGTG ATCGACTTCC 
     1321 GCAGTGGTGG GCGGCCTGAG GAGTACGAAG GGGAGTACCA GTGCTTTGCC CGGAACAAAT 
     1381 TCGGCACAGC TCTTAGCAAC CGCATCCGCC TGCAGGTGTC CAAATCTCCC CTGTGGCCCA 
     1441 AGGAAAACCT AGACCCCGTC GTGGTTCAAG AGGGTGCCCC CTTAACCCTG CAGTGCAACC 
     1501 CCCCACCTGG CCTCCCATCC CCCGTCATCT TCTGGATGAG CAGCTCCATG GAGCCCATCA 
     1561 CCCAGGACAA GCGTGTCTCC CAGGGTCACA ACGGGGACCT GTACTTCTCC AACGTCATGC 
     1621 TGCAGGACAT GCAGACCGAC TACAGCTGCA ATGCACGCTT CCACTTCACC CACACCATTC 
     1681 AGCAGAAGAA TCCCTTCACC CTCAAAGTCC TCACCAACAA CCCCTATAAT GACTCGTCTT 
     1741 TAAGAAACCA CCCTGACATA TATAGTGCCC GAGGAGTTGC GGAAAGAACA CCTAGCTTCA 
     1801 TGTATCCCCA GGGCACGTCA AGCAGTCAGA TGGTACTGCG CGGCATGGAC CTGCTGCTGG 
     1861 AGTGCATTGC CTCTGGGGTC CCAACACCAG ATATTGCATG GTACAAGAAA GGTGGGGACC 
     1921 TCCCATCTGA CAAGGCCAAG TTCGAGAATT TTAACAAGGC TCTGCGCATC ACCAATGTCT 
     1981 CTGAAGAGGA CTCTGGGGAG TATTTCTGCC TGGCCTCCAA CAAGATGGGC AGCATCCGGC 
     2041 ACACGATCTC GGTGAGAGTA AAGGCTGCCC CATACTGGCT GGATGAGCCC AAGAACTTGA 
     2101 TCCTGGCTCC TGGTGAAGAT GGGAGGCTGG TGTGTCGAGC CAATGGGAAC CCGAAGCCGA 
     2161 CCGTCCAGTG GCTGGTGAAT GGAGACCCTT TGCAATCGGC ACCACCCAAC CCCAACCGTG 
     2221 AGGTGGCCGG AGACACTATC ATCTTCCGGG ACACTCAGAT CAGCAGCAGG GCAGTGTACC 
     2281 AGTGTAACAC ATCCAACGAA CATGGCTACC TGCTGGCCAA TGCCTTCGTC AGCGTATTAG 
     2341 ATGTACCCCC TCGGATGCTG TCTCCCCGGA ACCAGCTCAT CAGGGTGATC CTTTACAACC 
     2401 GGACGCGACT GGACTGTCCG TTCTTTGGGT CTCCCATTCC AACACTCCGA TGGTTTAAGA 
     2461 ATGGGCAAGG AAGCAACCTG GATGGTGGTA ACTACCACGT CTACGAAAAT GGCAGCCTGG 
     2521 AAATCAAGAT GATTCGCAAA GAGGACCAAG GCATCTACAC CTGTGTGGCC ACCAACATCC 
     2581 TGGGAAAAGC TGAAAATCAA GTCCGCCTGG AGGTCAAAGA CCCCACCAGG ATCTACAGGA 
     2641 TGCCTGAAGA CCAGGTGGCC AAGAGGGGCA CCACAGTGCA GCTGGAGTGC CGTGTGAAGC 
     2701 ATGACCCCTC CTTGAAACTC ACAGTCTCCT GGCTGAAGGA TGACGAGCCA CTCTACATTG 
     2761 GAAACAGGAT GAAAAAGGAA GATGACTCCC TGACCATCTT CGGAGTGGCA GAGCGGGACC 
     2821 AAGGCAGTTA CACGTGCATG GCCAGCACCG AGCTGGACCA GGACCTGGCA AAGGCCTACC 
     2881 TCACTGTTCT AGGGCGGCCA GACCGACCCA GGGACCTGGA GCTCACTGAC CTGGCGGAAA 
     2941 GGAGTGTGAG GCTGACCTGG ATCCCGGGGG ATGATAACAA CAGTCCCATC ACAGACTACG 
     3001 TTGTCCAGTT TGAAGAGGAC CAGTTCCAGC CAGGAGTCTG GCATGACCAC TCCAAGTTCC 
     3061 CAGGCAGTGT CAACTCAGCC GTCCTCCATC TGTCCCCGTA TGTCAACTAT CAGTTCCGAG 
     3121 TCATCGCTGT CAACGAGGTT GGGAGCAGCC ACCCCAGCCT TCCATCCGAG CGGTACCGAA 
     3181 CAAGCGGGGC ACCCCCTGAA TCCAACCCCA GTGATGTGAA GGGCGAAGGG ACACGAAAAA 
     3241 ACAATATGGA GATCACATGG ACGCCTATGA ATGCTACCTC CGCCTTTGGC CCCAACCTGC 
     3301 GCTACATTGT CAAGTGGCGA CGGAGAGAAA CCCGAGAGAC TTGGAACAAT GTCACCGTGT 
     3361 GGGGCTCTCG CTATGTGGTG GGGCAGACCC CTGTCTACGT ACCCTATGAG ATCCGAGTCC 
     3421 AGGCTGAAAA TGACTTTGGG AAAGGCCCGG AGCCTGAAAC CGTCATTGGG TACTCGGGGG 
     3481 AAGATTATCC CAGGGCTGCA CCCACTGAAG TTAAAATCCG AGTCCTGAAC AGCACAGCCA 
     3541 TCAGCCTTCA GTGGAACCGC GTCTACCCTG ACACGGTCCA GGGCCAGCTC AGAGAGTATC 
     3601 GAGCTTACTA CTGGAGGGAA AGCAGTTTGC TGAAGAACCT GTGGGTGTCT CAGAAGAGAC 
     3661 AGCAGGCCAG CTTCCCTGGC GACCGCCCCC GGGGCGTGGT GGGTCGCCTG TTCCCCTACA 
     3721 GTAACTACAA GCTGGAGATG GTTGTGGTCA ATGGGAGAGG TGATGGGCCT CGAAGTGAAA 
     3781 CCAAGGAATT CACCACCCCG GAAGGAGTAC CCAGTGCCCC CAGGCGTTTC AGAGTCCGAC 
     3841 AGCCCAACCT GGAGACCATC AACCTGGAAT GGGATCACCC AGAGCACCCC AACGGGATCC 
     3901 TGATTGGATA CACGCTCAGA TACGTGCCCT TTAATGGAAC CAAACTGGGA AAGCAGATGG 
     3961 TGGAAAACTT CTCTCCCAAT CAGACCAAGT TCTCGGTGCA GAGAGCAGAC CCCGTGTCCC 
     4021 GTTACCGCTT CTCCCTCAGT GCCAGGACGC AGGTGGGCTC TGGAGAAGCA GCCACGGAGG 
     4081 AGTCCCCAAC ACCTCCAAAT GAAGCTACTC CAACTGCAGC TTACACCAAC AACCAGACTG 
     4141 ACATCGCCAC CCAGGGCTGG TTCATCGGGC TCATGTGTGC CATTGCCCTT CTGGTGCTGA 
     4201 TCCTGCTGAT CGTCTGCTTC ATCAAGAGGA GTCGTGGCGG CAAGTACCCA GTGCGAGAAA 
     4261 AGAAGGATGT CCCCTTGGGC CCTGAAGACC CCAAAGAAGA AGATGGTTCA TTTGACTACA 
     4321 GTGACGAGGA CAACAAGCCC CTGCAGGGCA GCCAGACATC TCTGGACGGC ACCATCAAGC 
     4381 AGCAGGAGAG TGACGACAGC CTAGTGGACT ACGGCGAGGG TGGCGAGGGC CAGTTCAACG 
     4441 AAGATGGCTC CTTTATTGGC CAGTACACTG TCAGAAAGGA CAAGGAGGAG ACCGAGGGCA 
     4501 ATGAGAGCTC AGAGGCCACA TCTCCAGTCA ATGCCATCTA TTCCCTGGCC ACGCGTACGC 
     4561 GGCCGCTCGA GCAGAAACTC ATCTCAGAAG AGGATCTGGC AGCAAATGAT ATCCTGGATT 
     4621 ACAAGGATGA CGACGATAAG GTTTAAACGG CCGGCCGCGG TCATAGCTGT TTCCTGAACA 
     4681 GATCCCGGGT GGCATCCCTG TGACCCCTCC CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC 
     4741 ACTCCAGTGC CCACCAGCCT TGTCCTAATA AAATTAAGTT GCATCATTTT GTCTGACTAG 
     4801 GTGTCCTTCT ATAATATTAT GGGGTGGAGG GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG 
     4861 AAGACAACCT GTAGGGCCTG CGGGGTCTAT TGGGAACCAA GCTGGAGTGC AGTGGCACAA 
     4921 TCTTGGCTCA CTGCAATCTC CGCCTCCTGG GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC 
     4981 GAGTTGTTGG GATTCCAGGC ATGCATGACC AGGCTCAGCT AATTTTTGTT TTTTTGGTAG 
     5041 AGACGGGGTT TCACCATATT GGCCAGGCTG GTCTCCAACT CCTAATCTCA GGTGATCTAC 
     5101 CCACCTTGGC CTCCCAAATT GCTGGGATTA CAGGCGTGAA CCACTGCTCC CTTCCCTGTC 
     5161 CTTCTGATTT TAAAATAACT ATACCAGCAG GAGGACGTCC AGACACAGCA TAGGCTACCT 
     5221 GGCCATGCCC AACCGGTGGG ACATTTGAGT TGCTTGCTTG GCACTGTCCT CTCATGCGTT 
     5281 GGGTCCACTC AGTAGATGCC TGTTGAATTG GGTACGCGGC CAGCGGCGAG CGGTATCAGC 
     5341 TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT 
     5401 GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT 
     5461 CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG 
     5521 AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC 
     5581 TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT 
     5641 GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA 
     5701 GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA 
     5761 TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA 
     5821 CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA 
     5881 CTACGGCTAC ACTAGAAGAA CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT 
     5941 CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT 
     6001 TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT 
     6061 CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT 
     6121 GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC 
     6181 AATCTAAAGT ATATATGAGT AACCTGAGGC TATGGCAGGG CCTGCCGCCC CGACGTTGGC 
     6241 TGCGAGCCCT GGGCCTTCAC CCGAACTTGG GGGGTGGGGT GGGGAAAAGG AAGAAACGCG 
     6301 GGCGTATTGG CCCCAATGGG GTCTCGGTGG GGTATCGACA GAGTGCCAGC CCTGGGACCG 
     6361 AACCCCGCGT TTATGAACAA ACGACCCAAC ACCGTGCGTT TTATTCTGTC TTTTTATTGC 
     6421 CGTCATAGCG CGGGTTCCTT CCGGTATTGT CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT 
     6481 AGGGTGGGCG AAGAACTCCA GCATGAGATC CCCGCGCTGG AGGATCATCC AGCCGGCGTC 
     6541 CCGGAAAACG ATTCCGAAGC CCAACCTTTC ATAGAAGGCG GCGGTGGAAT CGAAATCTCG 
     6601 TGATGGCAGG TTGGGCGTCG CTTGGTCGGT CATTTCGAAC CCCAGAGTCC CGCTCAGAAG 
     6661 AACTCGTCAA GAAGGCGATA GAAGGCGATG CGCTGCGAAT CGGGAGCGGC GATACCGTAA 
     6721 AGCACGAGGA AGCGGTCAGC CCATTCGCCG CCAAGCTCTT CAGCAATATC ACGGGTAGCC 
     6781 AACGCTATGT CCTGATAGCG ATCCGCCACA CCCAGCCGGC CACAGTCGAT GAATCCAGAA 
     6841 AAGCGGCCAT TTTCCACCAT GATATTCGGC AAGCAGGCAT CGCCATGGGT CACGACGAGA 
     6901 TCCTCGCCGT CGGGCATGCT CGCCTTGAGC CTGGCGAACA GTTCGGCTGG CGCGAGCCCC 
     6961 TGATGCTCTT CGTCCAGATC ATCCTGATCG ACAAGACCGG CTTCCATCCG AGTACGTGCT 
     7021 CGCTCGATGC GATGTTTCGC TTGGTGGTCG AATGGGCAGG TAGCCGGATC AAGCGTATGC 
     7081 AGCCGCCGCA TTGCATCAGC CATGATGGAT ACTTTCTCGG CAGGAGCAAG GTGAGATGAC 
     7141 AGGAGATCCT GCCCCGGCAC TTCGCCCAAT AGCAGCCAGT CCCTTCCCGC TTCAGTGACA 
     7201 ACGTCGAGCA CAGCTGCGCA AGGAACGCCC GTCGTGGCCA GCCACGATAG CCGCGCTGCC 
     7261 TCGTCTTGCA GTTCATTCAG GGCACCGGAC AGGTCGGTCT TGACAAAAAG AACCGGGCGC 
     7321 CCCTGCGCTG ACAGCCGGAA CACGGCGGCA TCAGAGCAGC CGATTGTCTG TTGTGCCCAG 
     7381 TCATAGCCGA ATAGCCTCTC CACCCAAGCG GCCGGAGAAC CTGCGTGCAA TCCATCTTGT 
     7441 TCAATCATGC GAAACGATCC TCATCCTGTC TCTTGATCGA TCTTTGCAAA AGCCTAGGCC 
     7501 TCCAAAAAAG CCTCCTCACT ACTTCTGGAA TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC 
     7561 TGCATAAATA AAAAAAATTA GTCAGCCATG GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT 
     7621 TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG GACTATGGTT GCTGACTAAT TGAGATGCAT 
     7681 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCTGG TTGCTGACTA 
     7741 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCC 
     7801 TAACTGACAC ACATTCCACA GCTGGTTCTT TCCGCCTCAG GACTCTTCCT TTTTCAATAT 
     7861 TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG 
     7921 AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC TGACGCGCCC 
     7981 TGTAGCGGCG CATTAAGCGC GGCGGGTGTG GTGGTTACGC GCAGCGTGAC CGCTACACTT 
     8041 GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT TTCTTCCCTT CCTTTCTCGC CACGTTCGCC 
     8101 GGCTTTCCCC GTCAAGCTCT AAATCGGGGG CTCCCTTTAG GGTTCCGATT TAGTGCTTTA 
     8161 CGGCACCTCG ACCCCAAAAA ACTTGATTAG GGTGATGGTT CACGTAGTGG GCCATCGCCC 
     8221 TGATAGACGG TTTTTCGCCC TTTGACGTTG GAGTCCACGT TCTTTAATAG TGGACTCTTG 
     8281 TTCCAAACTG GAACAACACT CAACCCTATC TCGGTCTATT CTTTTGATTT ATAAGGGATT 
     8341 TTGCCGATTT CGGCCTATTG GTTAAAAAAT GAGCTGATTT AACAAAAATT TAACGCGAAT 
     8401 TTT
//