LOCUS       RR214002                6455 bp    DNA     circular  SYN 
DEFINITION  Aldh2 (Myc-DDK-tagged ORF) - Rat aldehyde dehydrogenase 2 family (mitochondrial) (Aldh2), nuclear gene encoding mitochondrial protein, (10 ug)
ACCESSION   NM_032416
VERSION     NM_032416.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    2612..2703 
                     /label="Myc-DDK"  
     misc_feature    2733..3318 
                     /label="PolyA signal"  
     misc_feature    2790..2809 
                     /label="Restriction Enzyme"  
     misc_feature    3594..4266 
                     /label="ColE1"  
     misc_feature    4696..5490 
                     /label="Kan/Neo"  
     misc_feature    5525..5827 
                     /label="SV40 ori"  
     misc_feature    5913..6390 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    2594..2600 
                     /label="Restriction Enzyme"  
     misc_feature    1020..2594 
                     /label="Aldh2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCTGCGCGCC GCACTCAGCA CCGCCCGCCG TGGGCCACGC CTGAGCCGCC 
     1081 TGCTGTCCGC CGCCGCCACC AGCGCGGTGC CAGCCCCCAA CCAGCAGCCC GAGGTCTTCT 
     1141 GCAACCAGAT CTTCATTAAC AATGAGTGGC ATGATGCTGT CAGCAAGAAA ACATTCCCCA 
     1201 CCGTCAACCC TTCCACGGGG GAGGTCATCT GCCAGGTAGC CGAAGGGAAC AAGGAGGACG 
     1261 TAGACAAGGC AGTGAAGGCC GCTCAGGCAG CCTTCCAGCT GGGCTCGCCC TGGCGCCGCA 
     1321 TGGATGCATC TGACAGGGGC CGGCTGTTGT ACCGATTGGC TGATCTCATC GAACGGGACC 
     1381 GGACCTACCT GGCGGCCTTG GAGACCCTGG ACAACGGCAA GCCTTATGTC ATCTCCTACC 
     1441 TGGTGGATTT GGACATGGTT CTGAAATGTC TCCGCTATTA TGCTGGCTGG GCTGACAAGT 
     1501 ACCACGGGAA AACCATTCCC ATCGATGGCG ACTTCTTCAG CTACACCCGC CACGAGCCTG 
     1561 TGGGCGTGTG TGGACAGATC ATTCCGTGGA ACTTCCCGCT CCTGATGCAA GCCTGGAAGC 
     1621 TGGGCCCTGC CTTGGCAACT GGAAACGTGG TGGTGATGAA AGTGGCCGAG CAGACACCGC 
     1681 TCACTGCACT CTACGTGGCC AACTTGATCA AGGAGGCAGG CTTCCCCCCT GGTGTGGTCA 
     1741 ATATTGTTCC TGGATTCGGC CCTACCGCCG GGGCTGCCAT CGCGTCCCAC GAGGATGTGG 
     1801 ACAAAGTGGC CTTCACAGGT TCCACTGAGG TTGGTCACCT AATCCAGGTT GCCGCCGGGA 
     1861 GCAGCAATCT CAAGAGAGTA ACCCTGGAAC TGGGGGGAAA GAGCCCCAAT ATCATCATGT 
     1921 CAGACGCTGA CATGGACTGG GCTGTGGAAC AGGCCCACTT TGCCCTGTTC TTCAACCAGG 
     1981 GCCAGTGCTG TTGTGCGGGC TCCCGGACCT TCGTGCAGGA GGATGTGTAT GATGAATTCG 
     2041 TGGAACGCAG TGTGGCCCGG GCCAAGTCTC GGGTGGTCGG GAACCCTTTC GACAGCCGGA 
     2101 CGGAGCAGGG GCCGCAGGTG GATGAGACTC AGTTTAAGAA GATCCTGGGC TATATCAAGT 
     2161 CAGGACAACA AGAAGGGGCG AAGCTGCTGT GCGGTGGGGG CGCCGCCGCA GACCGTGGTT 
     2221 ACTTCATCCA GCCCACCGTG TTCGGAGACG TCAAAGATGG CATGACCATC GCCAAGGAGG 
     2281 AGATCTTCGG ACCAGTGATG CAGATCCTCA AATTCAAGAC CATTGAGGAG GTTGTGGGGC 
     2341 GAGCCAATAA TTCCAAGTAC GGGCTGGCTG CCGCTGTCTT CACAAAGGAC CTGGACAAGG 
     2401 CCAATTACCT GTCCCAAGCT CTGCAGGCTG GGACTGTGTG GATCAACTGC TACGATGTGT 
     2461 TTGGGGCCCA GTCCCCATTT GGTGGCTATA AGATGTCGGG GAGCGGCAGG GAGCTGGGCG 
     2521 AGTATGGCCT GCAGGCCTAC ACGGAAGTGA AGACGGTCAC CGTCAAAGTG CCACAGAAGA 
     2581 ACTCGTGCCA CAGAAGAACT CGACGCGTAC GCGGCCGCTC GAGCAGAAAC TCATCTCAGA 
     2641 AGAGGATCTG GCAGCAAATG ATATCCTGGA TTACAAGGAT GACGACGATA AGGTTTAAAC 
     2701 GGCCGGCCGC GGTCATAGCT GTTTCCTGAA CAGATCCCGG GTGGCATCCC TGTGACCCCT 
     2761 CCCCAGTGCC TCTCCTGGCC CTGGAAGTTG CCACTCCAGT GCCCACCAGC CTTGTCCTAA 
     2821 TAAAATTAAG TTGCATCATT TTGTCTGACT AGGTGTCCTT CTATAATATT ATGGGGTGGA 
     2881 GGGGGGTGGT ATGGAGCAAG GGGCAAGTTG GGAAGACAAC CTGTAGGGCC TGCGGGGTCT 
     2941 ATTGGGAACC AAGCTGGAGT GCAGTGGCAC AATCTTGGCT CACTGCAATC TCCGCCTCCT 
     3001 GGGTTCAAGC GATTCTCCTG CCTCAGCCTC CCGAGTTGTT GGGATTCCAG GCATGCATGA 
     3061 CCAGGCTCAG CTAATTTTTG TTTTTTTGGT AGAGACGGGG TTTCACCATA TTGGCCAGGC 
     3121 TGGTCTCCAA CTCCTAATCT CAGGTGATCT ACCCACCTTG GCCTCCCAAA TTGCTGGGAT 
     3181 TACAGGCGTG AACCACTGCT CCCTTCCCTG TCCTTCTGAT TTTAAAATAA CTATACCAGC 
     3241 AGGAGGACGT CCAGACACAG CATAGGCTAC CTGGCCATGC CCAACCGGTG GGACATTTGA 
     3301 GTTGCTTGCT TGGCACTGTC CTCTCATGCG TTGGGTCCAC TCAGTAGATG CCTGTTGAAT 
     3361 TGGGTACGCG GCCAGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC 
     3421 ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG 
     3481 AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT 
     3541 CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG 
     3601 GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA 
     3661 TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG 
     3721 TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT 
     3781 CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC 
     3841 GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC 
     3901 GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG AACAGTATTT 
     3961 GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC 
     4021 GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC 
     4081 AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG 
     4141 AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG 
     4201 ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA GTAACCTGAG 
     4261 GCTATGGCAG GGCCTGCCGC CCCGACGTTG GCTGCGAGCC CTGGGCCTTC ACCCGAACTT 
     4321 GGGGGGTGGG GTGGGGAAAA GGAAGAAACG CGGGCGTATT GGCCCCAATG GGGTCTCGGT 
     4381 GGGGTATCGA CAGAGTGCCA GCCCTGGGAC CGAACCCCGC GTTTATGAAC AAACGACCCA 
     4441 ACACCGTGCG TTTTATTCTG TCTTTTTATT GCCGTCATAG CGCGGGTTCC TTCCGGTATT 
     4501 GTCTCCTTCC GTGTTTCAGT TAGCCTCCCC CTAGGGTGGG CGAAGAACTC CAGCATGAGA 
     4561 TCCCCGCGCT GGAGGATCAT CCAGCCGGCG TCCCGGAAAA CGATTCCGAA GCCCAACCTT 
     4621 TCATAGAAGG CGGCGGTGGA ATCGAAATCT CGTGATGGCA GGTTGGGCGT CGCTTGGTCG 
     4681 GTCATTTCGA ACCCCAGAGT CCCGCTCAGA AGAACTCGTC AAGAAGGCGA TAGAAGGCGA 
     4741 TGCGCTGCGA ATCGGGAGCG GCGATACCGT AAAGCACGAG GAAGCGGTCA GCCCATTCGC 
     4801 CGCCAAGCTC TTCAGCAATA TCACGGGTAG CCAACGCTAT GTCCTGATAG CGATCCGCCA 
     4861 CACCCAGCCG GCCACAGTCG ATGAATCCAG AAAAGCGGCC ATTTTCCACC ATGATATTCG 
     4921 GCAAGCAGGC ATCGCCATGG GTCACGACGA GATCCTCGCC GTCGGGCATG CTCGCCTTGA 
     4981 GCCTGGCGAA CAGTTCGGCT GGCGCGAGCC CCTGATGCTC TTCGTCCAGA TCATCCTGAT 
     5041 CGACAAGACC GGCTTCCATC CGAGTACGTG CTCGCTCGAT GCGATGTTTC GCTTGGTGGT 
     5101 CGAATGGGCA GGTAGCCGGA TCAAGCGTAT GCAGCCGCCG CATTGCATCA GCCATGATGG 
     5161 ATACTTTCTC GGCAGGAGCA AGGTGAGATG ACAGGAGATC CTGCCCCGGC ACTTCGCCCA 
     5221 ATAGCAGCCA GTCCCTTCCC GCTTCAGTGA CAACGTCGAG CACAGCTGCG CAAGGAACGC 
     5281 CCGTCGTGGC CAGCCACGAT AGCCGCGCTG CCTCGTCTTG CAGTTCATTC AGGGCACCGG 
     5341 ACAGGTCGGT CTTGACAAAA AGAACCGGGC GCCCCTGCGC TGACAGCCGG AACACGGCGG 
     5401 CATCAGAGCA GCCGATTGTC TGTTGTGCCC AGTCATAGCC GAATAGCCTC TCCACCCAAG 
     5461 CGGCCGGAGA ACCTGCGTGC AATCCATCTT GTTCAATCAT GCGAAACGAT CCTCATCCTG 
     5521 TCTCTTGATC GATCTTTGCA AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA CTACTTCTGG 
     5581 AATAGCTCAG AGGCCGAGGC GGCCTCGGCC TCTGCATAAA TAAAAAAAAT TAGTCAGCCA 
     5641 TGGGGCGGAG AATGGGCGGA ACTGGGCGGA GTTAGGGGCG GGATGGGCGG AGTTAGGGGC 
     5701 GGGACTATGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG 
     5761 CCTGGGGACT TTCCACACCT GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG 
     5821 CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CCTAACTGAC ACACATTCCA CAGCTGGTTC 
     5881 TTTCCGCCTC AGGACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC 
     5941 TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA 
     6001 CATTTCCCCG AAAAGTGCCA CCTGACGCGC CCTGTAGCGG CGCATTAAGC GCGGCGGGTG 
     6061 TGGTGGTTAC GCGCAGCGTG ACCGCTACAC TTGCCAGCGC CCTAGCGCCC GCTCCTTTCG 
     6121 CTTTCTTCCC TTCCTTTCTC GCCACGTTCG CCGGCTTTCC CCGTCAAGCT CTAAATCGGG 
     6181 GGCTCCCTTT AGGGTTCCGA TTTAGTGCTT TACGGCACCT CGACCCCAAA AAACTTGATT 
     6241 AGGGTGATGG TTCACGTAGT GGGCCATCGC CCTGATAGAC GGTTTTTCGC CCTTTGACGT 
     6301 TGGAGTCCAC GTTCTTTAAT AGTGGACTCT TGTTCCAAAC TGGAACAACA CTCAACCCTA 
     6361 TCTCGGTCTA TTCTTTTGAT TTATAAGGGA TTTTGCCGAT TTCGGCCTAT TGGTTAAAAA 
     6421 ATGAGCTGAT TTAACAAAAA TTTAACGCGA ATTTT
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