LOCUS       RR213998                8466 bp    DNA     circular  SYN 
DEFINITION  Hipk2 (Myc-DDK-tagged ORF) - Rat homeodomain interacting protein kinase 2 (Hipk2), (10 ug)
ACCESSION   NM_001108622
VERSION     NM_001108622.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4623..4714 
                     /label="Myc-DDK"  
     misc_feature    4744..5329 
                     /label="PolyA signal"  
     misc_feature    4801..4820 
                     /label="Restriction Enzyme"  
     misc_feature    5605..6277 
                     /label="ColE1"  
     misc_feature    6707..7501 
                     /label="Kan/Neo"  
     misc_feature    7536..7838 
                     /label="SV40 ori"  
     misc_feature    7924..8401 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4605..4611 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4605 
                     /label="Hipk2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCCCCCGTG TACGAAGGTA TGGCCTCACA TGTGCAAGTT TTCTCCCCTC 
     1081 ACACCCTTCA ATCAAGTGCC TTCTGTAGTG TGAAGAAACT AAAAGTAGAG CCAAGTTCCA 
     1141 ACTGGGACAT GACTGGGTAC GGCTCCCACA GCAAAGTGTA CAGCCAGAGC AAGAACATAC 
     1201 CACCTTCTCA GCCAGCCTCC ACAACCGTCA GCACCTCCTT GCCAATCCCA AACCCAAGCC 
     1261 TACCTTACGA GCAGACCATC ATCTTCCCAG GAAGTACTGG ACACATAGTT GTAACATCAG 
     1321 CAAGTAGCAC TTCTGTCACC GGGCAAGTCC TCGGTGGACC ACATAACCTA ATGCGTCGAA 
     1381 GCACTGTGAG CCTCCTTGAC ACCTACCAAA AATGTGGACT CAAGCGCAAG AGTGAGGAGA 
     1441 TTGAGAACAC AAGCAGCGTG CAGATCATTG AGGAGCATCC ACCCATGATT CAGAATAATG 
     1501 CCAGCGGGGC CACTGTCGCC ACTGCCACCA CATCCACTGC CACCTCCAAA AACAGTGGCT 
     1561 CCAACAGTGA AGGTGACTAT CAGCTGGTGC AGCATGAAGT GCTGTGCTCT ATGACAAACA 
     1621 CCTATGAGGT TTTAGAGTTC TTGGGGCGAG GGACATTTGG GCAAGTGGTC AAGTGCTGGA 
     1681 AACGGGGCAC CAATGAAATT GTGGCCATTA AGATCCTAAA GAACCACCCA TCCTATGCCC 
     1741 GGCAAGGCCA GATTGAAGTG AGCATCCTGG CCCGGCTGAG CACGGAGAGC GCTGATGACT 
     1801 ACAATTTTGT GCGGGCCTAT GAGTGCTTCC AGCACAAGAA TCACACATGC TTAGTCTTTG 
     1861 AGATGTTGGA GCAGAACCTC TATGACTTTC TGAAACAGAA CAAGTTTAGT CCCTTGCCCC 
     1921 TCAAGTATAT TCGCCCAGTC CTCCAGCAGG TAGCCACAGC CCTGATGAAA CTCAAAAGCC 
     1981 TGGGTCTTAT CCATGCTGAC CTCAAACCAG AAAACATCAT GCTGGTGGAT CCATCCAGAC 
     2041 AACCATACCG AGTGAAGGTC ATTGACTTTG GTTCAGCTAG TCATGTGTCC AAAGCTGTCT 
     2101 GTTCTACGTA CTTGCAATCC AGATACTACA GGGCCCCTGA AATCATCCTT GGTTTGCCAT 
     2161 TCTGTGAGGC AATTGACATG TGGTCCCTGG GTTGTGTCAT TGCTGAGCTG TTCCTGGGCT 
     2221 GGCCATTATA CCCAGGTGCT TCTGAGTATG ATCAGATTCG GTATATTTCA CAAACACAGG 
     2281 GTTTACCAGC TGAATATTTA TTAAGTGCAG GGACAAAGAC AACTAGGTTT TTCAACCGTG 
     2341 ACACAGACTC ACCGTATCCT TTGTGGAGGC TAAAGACACC AGATGACCAT GAAGCAGAGA 
     2401 CGGGGATTAA ATCAAAAGAA GCAAGAAAGT ACATTTTCAA CTGTTTGGAT GACATGGCCC 
     2461 AGGTGAACAT GACAACAGAT TTGGAAGGGA GTGACATGTT AGTAGAGAAG GCAGACCGGC 
     2521 GGGAGTTCAT TGACCTGTTA AAGAAGATGC TGACCATAGA TGCTGATAAG AGGATCACTC 
     2581 CCATTGAAAC TCTGAACCAC CCCTTTGTCA CCATGACGCA CCTACTGGAT TTTCCTCATA 
     2641 GTACTCATGT CAAATCTTGT TTCCAAAACA TGGAGATTTG CAAGCGCCGG GTGAATATGT 
     2701 ATGACACAGT GAACCAGAGC AAAACACCTT TCATCACACA TGTGGCCCCC AGCACATCTA 
     2761 CCAACTTGAC CATGACCTTT AACAACCAGC TGACCACTGT CCACAACCAG GCTCCCACCT 
     2821 CCACCAGTGC CACTCTTTCC TTAGCCAATC CCGAAGTCTC CATACTAAAC TACCAATCTA 
     2881 CACTCTACCA GCCCTCAGCG GCATCCATGG CCGCAGTGGC CCAGCGGAGC ATGCCCCTGC 
     2941 AGACGGGAAC AGCCCAAATT TGTGCCCGGC CTGACCCTTT CCAGCAAGCT CTCATCGTGT 
     3001 GTCCCCCTGG CTTCCAAGGC CTGCAGGCCT CTCCCTCCAA GCACGCTGGC TACTCAGTGC 
     3061 GGATGGAAAA TGCTGTTCCC ATCGTCACTC AGGCTCCGGG CGCTCAGCCT CTTCAGATCC 
     3121 AGCCAGGTCT GCTTGCTCAG GCCTGGCCAG GTGGGGCCCA ACAGATCCTC CTTCCTCCAG 
     3181 CATGGCAGCA GCTCACTGGC GTGGCTACCC ACACATCCGT ACAGCACGCA GCTGTGATTC 
     3241 CCGAGACCAT GGCAGGCACT CAGCAACTGG CTGACTGGAG AAACACACAT GCTCACGGAA 
     3301 GCCATTACAA TCCCATCATG CAGCAGCCTG CACTCTTGAC TGGTCATGTG ACCCTTCCAG 
     3361 CTGCCCAGCC CTTAAATGTG GGTGTAGCCC ACGTGATGAG ACAGCAACCA ACCAGCACCA 
     3421 CCTCCTCCAG GAAGAGTAAG CAGCACCAAT CATCAGCGAG AAATGTCTCC ACCTGTGAAG 
     3481 TGACCTCTTC CCAGGCTATC AGCTCCCCTC AGCGATCCAA GCGTGTCAAG GAGAACACTC 
     3541 CCCCGAGGTG TGCAATGGTA CACAGCAGCC CAGCTTGCAG CACCTCACTC ACCTGTGGGT 
     3601 GGGGTGACGG GGCCTCCAGC ACCACCCGGG ACCGACAGCG GCAGACCATT GTCATCCCCG 
     3661 ACACCCCGAG CCCCACAGTC AGTGTCATCA CCATCAGCAG CGACACAGAT GAGGAGGAGG 
     3721 AACAGAAGCA CGCCCCCACC AGCACAGTCT CCAAGCAAAG AAAAAATGTC ATCAGCTGTG 
     3781 TCACAGTCCA TGATTCTCCC TACTCTGACT CCTCCAGCAA CACCAGCCCC TACTCGGTGC 
     3841 AGCAGCGCAC AGGGCACAAC GGCACCAACA CCTTGGACAC CAAGGGGGGC CTAGAGAACC 
     3901 ACTGCACCGG CAACCCCCGC ACCATCATCG TACCCCCACT GAAGACGCAG GCCAGTGAAG 
     3961 TGTTGGTAGA ATGTGACAGC CTAGGGCCAG CAATCAGTAC CGGTCACCAT TCATCCTCCT 
     4021 TCAAGTCCAA GTCCTCCAGC ACCGTGACCT CCACCAGTGG CCACTCTTCA GGGAGCTCGT 
     4081 CAGGAGCCAT CGCCTACCGT CAACAGCGGC CAGGCCCCCA CTTCCAGCAG CAGCAGCCCC 
     4141 TCAATCTCAG CCAGGCTCAG CAGCACATGG CTGCAGACCG CACTGGGAGT CACCGTCGGC 
     4201 AGCAGGCCTA CATCACTCCT ACCATGGCTC AGGCTCCGTA CACCTTCCCG CACAGCAGCC 
     4261 CCAGCCATGG CACTGTTCAC CCCCACCTGG CTGCGGCTGC CCACCTCCCT GCCCAGCCTC 
     4321 ACCTCTACAC CTACACAGCG CCCACAGCCC TAGGCTCCAC CAGCACTGTA GCCCACCTGG 
     4381 TGGCATCCCA AGGCTCAGCA CGACACACCG TGCAGCACAC TGCCTATCCG GCCAGCATCG 
     4441 TCCACCAGGT CCCCGTGAGC ATGGGGCCCC GGGTCCTGCC CTCGCCCACC ATTCACCCCA 
     4501 GTCAGTATCC GGCCCAGTTT GCCCACCAGA CCTATATCAG CGCCTCGCCA GCCTCCACCG 
     4561 TCTACACTGG ATATCCACTG AGTCCTGCCA AGGTCAACCA ATACCCTTAC ATAACGCGTA 
     4621 CGCGGCCGCT CGAGCAGAAA CTCATCTCAG AAGAGGATCT GGCAGCAAAT GATATCCTGG 
     4681 ATTACAAGGA TGACGACGAT AAGGTTTAAA CGGCCGGCCG CGGTCATAGC TGTTTCCTGA 
     4741 ACAGATCCCG GGTGGCATCC CTGTGACCCC TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT 
     4801 GCCACTCCAG TGCCCACCAG CCTTGTCCTA ATAAAATTAA GTTGCATCAT TTTGTCTGAC 
     4861 TAGGTGTCCT TCTATAATAT TATGGGGTGG AGGGGGGTGG TATGGAGCAA GGGGCAAGTT 
     4921 GGGAAGACAA CCTGTAGGGC CTGCGGGGTC TATTGGGAAC CAAGCTGGAG TGCAGTGGCA 
     4981 CAATCTTGGC TCACTGCAAT CTCCGCCTCC TGGGTTCAAG CGATTCTCCT GCCTCAGCCT 
     5041 CCCGAGTTGT TGGGATTCCA GGCATGCATG ACCAGGCTCA GCTAATTTTT GTTTTTTTGG 
     5101 TAGAGACGGG GTTTCACCAT ATTGGCCAGG CTGGTCTCCA ACTCCTAATC TCAGGTGATC 
     5161 TACCCACCTT GGCCTCCCAA ATTGCTGGGA TTACAGGCGT GAACCACTGC TCCCTTCCCT 
     5221 GTCCTTCTGA TTTTAAAATA ACTATACCAG CAGGAGGACG TCCAGACACA GCATAGGCTA 
     5281 CCTGGCCATG CCCAACCGGT GGGACATTTG AGTTGCTTGC TTGGCACTGT CCTCTCATGC 
     5341 GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA TTGGGTACGC GGCCAGCGGC GAGCGGTATC 
     5401 AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA 
     5461 CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT 
     5521 TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG 
     5581 GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG 
     5641 CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG 
     5701 CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC 
     5761 CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA 
     5821 CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG 
     5881 TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC 
     5941 TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC 
     6001 CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG 
     6061 TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT 
     6121 GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT 
     6181 CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA 
     6241 ATCAATCTAA AGTATATATG AGTAACCTGA GGCTATGGCA GGGCCTGCCG CCCCGACGTT 
     6301 GGCTGCGAGC CCTGGGCCTT CACCCGAACT TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC 
     6361 GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG TGGGGTATCG ACAGAGTGCC AGCCCTGGGA 
     6421 CCGAACCCCG CGTTTATGAA CAAACGACCC AACACCGTGC GTTTTATTCT GTCTTTTTAT 
     6481 TGCCGTCATA GCGCGGGTTC CTTCCGGTAT TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC 
     6541 CCTAGGGTGG GCGAAGAACT CCAGCATGAG ATCCCCGCGC TGGAGGATCA TCCAGCCGGC 
     6601 GTCCCGGAAA ACGATTCCGA AGCCCAACCT TTCATAGAAG GCGGCGGTGG AATCGAAATC 
     6661 TCGTGATGGC AGGTTGGGCG TCGCTTGGTC GGTCATTTCG AACCCCAGAG TCCCGCTCAG 
     6721 AAGAACTCGT CAAGAAGGCG ATAGAAGGCG ATGCGCTGCG AATCGGGAGC GGCGATACCG 
     6781 TAAAGCACGA GGAAGCGGTC AGCCCATTCG CCGCCAAGCT CTTCAGCAAT ATCACGGGTA 
     6841 GCCAACGCTA TGTCCTGATA GCGATCCGCC ACACCCAGCC GGCCACAGTC GATGAATCCA 
     6901 GAAAAGCGGC CATTTTCCAC CATGATATTC GGCAAGCAGG CATCGCCATG GGTCACGACG 
     6961 AGATCCTCGC CGTCGGGCAT GCTCGCCTTG AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC 
     7021 CCCTGATGCT CTTCGTCCAG ATCATCCTGA TCGACAAGAC CGGCTTCCAT CCGAGTACGT 
     7081 GCTCGCTCGA TGCGATGTTT CGCTTGGTGG TCGAATGGGC AGGTAGCCGG ATCAAGCGTA 
     7141 TGCAGCCGCC GCATTGCATC AGCCATGATG GATACTTTCT CGGCAGGAGC AAGGTGAGAT 
     7201 GACAGGAGAT CCTGCCCCGG CACTTCGCCC AATAGCAGCC AGTCCCTTCC CGCTTCAGTG 
     7261 ACAACGTCGA GCACAGCTGC GCAAGGAACG CCCGTCGTGG CCAGCCACGA TAGCCGCGCT 
     7321 GCCTCGTCTT GCAGTTCATT CAGGGCACCG GACAGGTCGG TCTTGACAAA AAGAACCGGG 
     7381 CGCCCCTGCG CTGACAGCCG GAACACGGCG GCATCAGAGC AGCCGATTGT CTGTTGTGCC 
     7441 CAGTCATAGC CGAATAGCCT CTCCACCCAA GCGGCCGGAG AACCTGCGTG CAATCCATCT 
     7501 TGTTCAATCA TGCGAAACGA TCCTCATCCT GTCTCTTGAT CGATCTTTGC AAAAGCCTAG 
     7561 GCCTCCAAAA AAGCCTCCTC ACTACTTCTG GAATAGCTCA GAGGCCGAGG CGGCCTCGGC 
     7621 CTCTGCATAA ATAAAAAAAA TTAGTCAGCC ATGGGGCGGA GAATGGGCGG AACTGGGCGG 
     7681 AGTTAGGGGC GGGATGGGCG GAGTTAGGGG CGGGACTATG GTTGCTGACT AATTGAGATG 
     7741 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC TGGTTGCTGA 
     7801 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     7861 CCCTAACTGA CACACATTCC ACAGCTGGTT CTTTCCGCCT CAGGACTCTT CCTTTTTCAA 
     7921 TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT 
     7981 TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC ACCTGACGCG 
     8041 CCCTGTAGCG GCGCATTAAG CGCGGCGGGT GTGGTGGTTA CGCGCAGCGT GACCGCTACA 
     8101 CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC GCTTTCTTCC CTTCCTTTCT CGCCACGTTC 
     8161 GCCGGCTTTC CCCGTCAAGC TCTAAATCGG GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT 
     8221 TTACGGCACC TCGACCCCAA AAAACTTGAT TAGGGTGATG GTTCACGTAG TGGGCCATCG 
     8281 CCCTGATAGA CGGTTTTTCG CCCTTTGACG TTGGAGTCCA CGTTCTTTAA TAGTGGACTC 
     8341 TTGTTCCAAA CTGGAACAAC ACTCAACCCT ATCTCGGTCT ATTCTTTTGA TTTATAAGGG 
     8401 ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA AATGAGCTGA TTTAACAAAA ATTTAACGCG 
     8461 AATTTT
//