LOCUS       RR213904                6882 bp    DNA     circular  SYN 
DEFINITION  Tgm4 (Myc-DDK-tagged ORF) - Rat transglutaminase 4 (prostate) (Tgm4), (10 ug)
ACCESSION   NM_022713
VERSION     NM_022713.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3039..3130 
                     /label="Myc-DDK"  
     misc_feature    3160..3745 
                     /label="PolyA signal"  
     misc_feature    3217..3236 
                     /label="Restriction Enzyme"  
     misc_feature    4021..4693 
                     /label="ColE1"  
     misc_feature    5123..5917 
                     /label="Kan/Neo"  
     misc_feature    5952..6254 
                     /label="SV40 ori"  
     misc_feature    6340..6817 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3021..3027 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3021 
                     /label="Tgm4"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGACTCCAGG AACATGCTTG TCGTTTACTC TGTGAACTTG GAGAAAAAGC 
     1081 TGAACGCTGC TGCCCACCAC ACGATTGAGT ACCAAACTCA GAAGCTCGTG CTCAGGCGTG 
     1141 GACAGATATT CTCCCTGAAG GTGATGCTAA ACAGACCGCT CCAATCTCAT GATGAACTGA 
     1201 AGCTGATATT CAATACTGGA CACAACATGC CATTCTACAC TGTGGAGCTT GATCCAATGA 
     1261 CATCCTACCG TTCCAAGGGC TGGCAGGTGA AGATTGCTAA ACAGTCTGGT GTAGAGGTTG 
     1321 TATTGAATGT CATCAGTGCT GCCGACGCCG TGGTGGGGAG GTACACGATG AACGTGAACG 
     1381 AATTTGACGC TGGAGTGTTC TTCCTTCTCT TCAATCCGTG GTGCTCAGAT GACAGTGTCT 
     1441 TCATGGCTAG TGAGGAAGAC CGAGCAGAGT ACGTCCTTAA TGATACCGGT TACATGTACA 
     1501 TGGGTTTTGC CAAACAGATT AAAGAGAAGC CCTGGACCTT TGGTCAGTTT GAGAAGTACA 
     1561 TCTTGAACTG CTGCTTCCGA TTGTTGACCC ATTTGGAGCC CAAGGAAATG CAGAGCCCAG 
     1621 TGCTTGTGTC CAGGGCCATT TGTACTATGA TGTGTGCTGC AAACAATTTT GGAGTGCTGG 
     1681 TGGGGAACTG GACAGGAGAC TATAGCAATG GCACTGCCCC GTATGTGTGG GCCAGCAGTG 
     1741 TACCCATTCT CCAGCAGCAC TACATCACCA GGATGCCTGT ACGCTTTGGC CAGTGCTGGG 
     1801 TTTTCTCTGG AGTCCTGACC ACAGCACTGA GAGCAGTGGG CATCCCAGCT CGCAGCGTGA 
     1861 CTAACTTTGA GTCAGCCCAC GATACAGAAA AGAACCTCAG AGTAGACATC TACCTGGATG 
     1921 AATCGGGCAA GACAATTCCA CATCTGACCA AAGACTCTGT CTGGAATTTC CATGTGTGGA 
     1981 CAGATGCCTG GATGAAAAGA CAGGATCTAC CCCAGGGCCA TGATGGTTGG CAGGTCCTGG 
     2041 ATTCTACACC ACAGGAAATC AGTGAAGGAC AATTCCGCAT TGGACCTTCA CCGGTGTCTG 
     2101 CTATCCGCCA GGGATTGGTC CAAATCATGT ATGACACCAC ATTTGTGTTC ACGGAGGTGA 
     2161 ACGGTGACAA GTACATCTGG CTGGTGAAGC AGAACCAGGA AAGGGAGAAG AATGTCCTCA 
     2221 TCGCTGTGGA GACTGCTAGC ATCGGCAAGA ACATCAGCAC CAAGATGGTG GGCGAGAACA 
     2281 GGCGTCAAGA TATTACCTTG CATTACAAGT TCCCAGAAGG CAGCCCAGAG GAGAGGAAGG 
     2341 CCATGGAGAA GGCCTCTGGG AAACGTCCTG ATGACAAGCT CAACTCTCGG ACACTTCACA 
     2401 TATCTGTCCT CCAGAACTCT GTGGAGCTAG GACATCCTAT CAATTTGACC ATAGTGTTGA 
     2461 AAAGGAAAAC GGCCACACCA CAGAATGTCA ACATTTCATG CTCCCTTGAC CTACAGACTT 
     2521 ACACAGGCAA CAAGAAGACA AACCTGGGAG TCATCCAGAA GACTGTGCAG ATTCAAGGCC 
     2581 AAGAATCAGA GGTGTCTCTC AGCATGGATT CCAGTTTCTA CATCTATAAA CTGGGCATGG 
     2641 TTGATGATGA GATGGTCATT AAAGGGTTCA TCATTGCAGA AATTGTTGAT TCTGGTGAAC 
     2701 GGGTAGCCAC TGATACGACC CTGTGTTTCC TGTACTCTGC CTTCTCTGTA GAGATGCCGA 
     2761 GCACAAGCAA GGTCAACCAG CCTCTGACCA TCACCTGCAA CTTCAAGAAC ACCCTGCCCA 
     2821 TCCCTTTGAC TAACATCAAG TTCTCAGTGG AAAGCCTGGG CCTCAATAAC ATGAAGTCCT 
     2881 GGGAGCAAGA GACGGTGCCG CCCGGAAAGA CCATCAATTT CCAGATAGAA TGCACCCCAG 
     2941 TGAAAACTGG ACCCAGGAAG TTCATTGTCA AGTTTATTTC CAGACAAGTG AAAGAGGTTC 
     3001 ATGCCGAGAA GGTCGTTCTC ATCACAAAGA CGCGTACGCG GCCGCTCGAG CAGAAACTCA 
     3061 TCTCAGAAGA GGATCTGGCA GCAAATGATA TCCTGGATTA CAAGGATGAC GACGATAAGG 
     3121 TTTAAACGGC CGGCCGCGGT CATAGCTGTT TCCTGAACAG ATCCCGGGTG GCATCCCTGT 
     3181 GACCCCTCCC CAGTGCCTCT CCTGGCCCTG GAAGTTGCCA CTCCAGTGCC CACCAGCCTT 
     3241 GTCCTAATAA AATTAAGTTG CATCATTTTG TCTGACTAGG TGTCCTTCTA TAATATTATG 
     3301 GGGTGGAGGG GGGTGGTATG GAGCAAGGGG CAAGTTGGGA AGACAACCTG TAGGGCCTGC 
     3361 GGGGTCTATT GGGAACCAAG CTGGAGTGCA GTGGCACAAT CTTGGCTCAC TGCAATCTCC 
     3421 GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA 
     3481 TGCATGACCA GGCTCAGCTA ATTTTTGTTT TTTTGGTAGA GACGGGGTTT CACCATATTG 
     3541 GCCAGGCTGG TCTCCAACTC CTAATCTCAG GTGATCTACC CACCTTGGCC TCCCAAATTG 
     3601 CTGGGATTAC AGGCGTGAAC CACTGCTCCC TTCCCTGTCC TTCTGATTTT AAAATAACTA 
     3661 TACCAGCAGG AGGACGTCCA GACACAGCAT AGGCTACCTG GCCATGCCCA ACCGGTGGGA 
     3721 CATTTGAGTT GCTTGCTTGG CACTGTCCTC TCATGCGTTG GGTCCACTCA GTAGATGCCT 
     3781 GTTGAATTGG GTACGCGGCC AGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG 
     3841 GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA 
     3901 GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA 
     3961 CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG 
     4021 ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT 
     4081 TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG 
     4141 CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC 
     4201 CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT 
     4261 AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA 
     4321 TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC 
     4381 AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC 
     4441 TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT 
     4501 TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC 
     4561 TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT 
     4621 CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA 
     4681 ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC GACGTTGGCT GCGAGCCCTG GGCCTTCACC 
     4741 CGAACTTGGG GGGTGGGGTG GGGAAAAGGA AGAAACGCGG GCGTATTGGC CCCAATGGGG 
     4801 TCTCGGTGGG GTATCGACAG AGTGCCAGCC CTGGGACCGA ACCCCGCGTT TATGAACAAA 
     4861 CGACCCAACA CCGTGCGTTT TATTCTGTCT TTTTATTGCC GTCATAGCGC GGGTTCCTTC 
     4921 CGGTATTGTC TCCTTCCGTG TTTCAGTTAG CCTCCCCCTA GGGTGGGCGA AGAACTCCAG 
     4981 CATGAGATCC CCGCGCTGGA GGATCATCCA GCCGGCGTCC CGGAAAACGA TTCCGAAGCC 
     5041 CAACCTTTCA TAGAAGGCGG CGGTGGAATC GAAATCTCGT GATGGCAGGT TGGGCGTCGC 
     5101 TTGGTCGGTC ATTTCGAACC CCAGAGTCCC GCTCAGAAGA ACTCGTCAAG AAGGCGATAG 
     5161 AAGGCGATGC GCTGCGAATC GGGAGCGGCG ATACCGTAAA GCACGAGGAA GCGGTCAGCC 
     5221 CATTCGCCGC CAAGCTCTTC AGCAATATCA CGGGTAGCCA ACGCTATGTC CTGATAGCGA 
     5281 TCCGCCACAC CCAGCCGGCC ACAGTCGATG AATCCAGAAA AGCGGCCATT TTCCACCATG 
     5341 ATATTCGGCA AGCAGGCATC GCCATGGGTC ACGACGAGAT CCTCGCCGTC GGGCATGCTC 
     5401 GCCTTGAGCC TGGCGAACAG TTCGGCTGGC GCGAGCCCCT GATGCTCTTC GTCCAGATCA 
     5461 TCCTGATCGA CAAGACCGGC TTCCATCCGA GTACGTGCTC GCTCGATGCG ATGTTTCGCT 
     5521 TGGTGGTCGA ATGGGCAGGT AGCCGGATCA AGCGTATGCA GCCGCCGCAT TGCATCAGCC 
     5581 ATGATGGATA CTTTCTCGGC AGGAGCAAGG TGAGATGACA GGAGATCCTG CCCCGGCACT 
     5641 TCGCCCAATA GCAGCCAGTC CCTTCCCGCT TCAGTGACAA CGTCGAGCAC AGCTGCGCAA 
     5701 GGAACGCCCG TCGTGGCCAG CCACGATAGC CGCGCTGCCT CGTCTTGCAG TTCATTCAGG 
     5761 GCACCGGACA GGTCGGTCTT GACAAAAAGA ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC 
     5821 ACGGCGGCAT CAGAGCAGCC GATTGTCTGT TGTGCCCAGT CATAGCCGAA TAGCCTCTCC 
     5881 ACCCAAGCGG CCGGAGAACC TGCGTGCAAT CCATCTTGTT CAATCATGCG AAACGATCCT 
     5941 CATCCTGTCT CTTGATCGAT CTTTGCAAAA GCCTAGGCCT CCAAAAAAGC CTCCTCACTA 
     6001 CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC CTCGGCCTCT GCATAAATAA AAAAAATTAG 
     6061 TCAGCCATGG GGCGGAGAAT GGGCGGAACT GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT 
     6121 TAGGGGCGGG ACTATGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     6181 TGGGGAGCCT GGGGACTTTC CACACCTGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     6241 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCCT AACTGACACA CATTCCACAG 
     6301 CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT 
     6361 TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT 
     6421 CCGCGCACAT TTCCCCGAAA AGTGCCACCT GACGCGCCCT GTAGCGGCGC ATTAAGCGCG 
     6481 GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT 
     6541 CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA 
     6601 AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA 
     6661 CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT 
     6721 TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC 
     6781 AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG 
     6841 TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TT
//