LOCUS       RR213877                6717 bp    DNA     circular  SYN 
DEFINITION  Tbx18 (Myc-DDK-tagged ORF) - Rat T-box18 (Tbx18), (10 ug)
ACCESSION   NM_001108173
VERSION     NM_001108173.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    2874..2965 
                     /label="Myc-DDK"  
     misc_feature    2995..3580 
                     /label="PolyA signal"  
     misc_feature    3052..3071 
                     /label="Restriction Enzyme"  
     misc_feature    3856..4528 
                     /label="ColE1"  
     misc_feature    4958..5752 
                     /label="Kan/Neo"  
     misc_feature    5787..6089 
                     /label="SV40 ori"  
     misc_feature    6175..6652 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    2856..2862 
                     /label="Restriction Enzyme"  
     misc_feature    1020..2856 
                     /label="Tbx18"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGGAGAAG CGGAGGGGCT CACCGTGCAG CATGCTAAGC CTCAAGGCGC 
     1081 ATGCCTTCTC GGTGGAGGCA CTGATCGGCG CCGAAAAGCA GCAACAGCTT CAAAAGAAGC 
     1141 GGAGAAAGCT GGCCACGGAA GAGGCGGCCG GGGCGGTGGA CGACGCAGGC TGCAGCCGTG 
     1201 GCGGAGGCGC GAGAGAACAC CGCTGCACCG AGGCCGACGA AGACCCGGCT CCCCCGCCGC 
     1261 CCGCAGCCGG GTCGGCGTCC GGGCCGGCGC GGAGCTGCAC GGACGCCGAA CGGAGCTGTG 
     1321 CTTCCCGCGG ACCCGCGGGC AGCTGTGAGG ACGGTTTCTT GCAGGGCGCT TCCCCGCTGG 
     1381 CGTCCCCGGG AGGTTCCCCG AAAGGGTCTC CGGTACCTGG CCTGGCACGG CCGGGGACCC 
     1441 CGCTGCCCGC GCCACAGGCC CCAAGAGTGG ATCTGCAAGG AGCAGAACTC TGGAAACGCT 
     1501 TTCACGAAAT AGGCACCGAG ATGATCATCA CCAAAGCCGG CAGGCGCATG TTTCCAGCGA 
     1561 TGCGAGTGAA GATCTCCGGG TTAGATCCTC ACCAACAATA TTACATTGCC ATGGATATTG 
     1621 TGCCCGTGGA CAACAAAAGA TACAGATATG TTTACCACAG CTCTAAATGG ATGGTGGCAG 
     1681 GAAATGCTGA TTCCCCGGTG CCACCCCGAG TATACATTCA TCCAGACTCA CCGGCCTCTG 
     1741 GGGAGACTTG GATGAGACAA GTGATCAGCT TCGACAAGCT GAAACTTACC AACAACGAAC 
     1801 TGGATGACCA AGGCCATATC ATTCTTCATT CTATGCACAA ATACCAACCG CGAGTGCACA 
     1861 TCATCCGTAA AGACTGCGGG GACGATCTGT CCCCCATCAA GCCCGTTCCC TCGGGAGAGG 
     1921 GAGTGAAGGC GTTCTCCTTT CCAGAAACAG TCTTCACAAC TGTCACTGCC TATCAGAATC 
     1981 AGCAGATTAC TCGCCTGAAG ATAGACAGGA ATCCATTTGC CAAAGGCTTC CGAGACTCTG 
     2041 GGAGGAACAG AATGGGTTTG GAAGCTCTGG TGGAGTCATA TGCATTCTGG AGGCCATCAC 
     2101 TACGAACTCT CACCTTTGAA GATATTCCTG GAATCCCAAA GCAAGGCAAC ACAAGTTCTT 
     2161 CAGCTCTACT CCCAGGTACT GGGAATGGCG TTCCTGCTGC GCACCCTCAC CTGCTGTCTG 
     2221 GCTCCTCCTG TTCCTCTCCT GCCTTCCATC TGGGGCCCAA CACCAGCCAG CTGTGTAGTA 
     2281 TGGCTCCCGC TGACTATTCT GCCTGCGCCC GGTCAGGCCT TGCTCTCAAC CGATACAGCA 
     2341 CGTCCTTGGC AGAGACCTAC AACAGGCTTA CCAACCAGAG CAGCGAGACC TTTGCCCCAC 
     2401 CCAGGACTCC CTCCTATGTG AGTGTGAGCA GCCCCTCTGT GAACATGTCC ATGGGCGGCA 
     2461 CGGACGGGGA CACCTTCAGC TGCCCGCAGA CCAGCCTGTC CATGCAGATT TCAGGAATGT 
     2521 CCCCTCAACT GCAGTACATC ATCCCGTCAC CATCCAGCAA TGCCTTTGCT GCTAACCAGA 
     2581 CCCATCAGGG TTCCTACAAC ACTTTCCGAT TGCACAGTCC CTGTGCCTTG TATGGATATA 
     2641 ACTTCTCCAC ATCCCCCAAA CTGGCTGCCA GTCCTGAGAA AATTGTCTCT TCCCAAGGAA 
     2701 GTTTCTTGGG GTCGTCACCG AGTGGGACCA TGACGGATCG GCAGATGTTG CCCCCGGTGG 
     2761 AAGGAGTGCA CCTGCTCGGC AGTGGGAACC AGCAGAGTTT CTTTGACTCC AGGACCCTAG 
     2821 GAAGCTTGAC TCTGTCATCT TCTCAAGTGT CTGCGCATAT GGTCACGCGT ACGCGGCCGC 
     2881 TCGAGCAGAA ACTCATCTCA GAAGAGGATC TGGCAGCAAA TGATATCCTG GATTACAAGG 
     2941 ATGACGACGA TAAGGTTTAA ACGGCCGGCC GCGGTCATAG CTGTTTCCTG AACAGATCCC 
     3001 GGGTGGCATC CCTGTGACCC CTCCCCAGTG CCTCTCCTGG CCCTGGAAGT TGCCACTCCA 
     3061 GTGCCCACCA GCCTTGTCCT AATAAAATTA AGTTGCATCA TTTTGTCTGA CTAGGTGTCC 
     3121 TTCTATAATA TTATGGGGTG GAGGGGGGTG GTATGGAGCA AGGGGCAAGT TGGGAAGACA 
     3181 ACCTGTAGGG CCTGCGGGGT CTATTGGGAA CCAAGCTGGA GTGCAGTGGC ACAATCTTGG 
     3241 CTCACTGCAA TCTCCGCCTC CTGGGTTCAA GCGATTCTCC TGCCTCAGCC TCCCGAGTTG 
     3301 TTGGGATTCC AGGCATGCAT GACCAGGCTC AGCTAATTTT TGTTTTTTTG GTAGAGACGG 
     3361 GGTTTCACCA TATTGGCCAG GCTGGTCTCC AACTCCTAAT CTCAGGTGAT CTACCCACCT 
     3421 TGGCCTCCCA AATTGCTGGG ATTACAGGCG TGAACCACTG CTCCCTTCCC TGTCCTTCTG 
     3481 ATTTTAAAAT AACTATACCA GCAGGAGGAC GTCCAGACAC AGCATAGGCT ACCTGGCCAT 
     3541 GCCCAACCGG TGGGACATTT GAGTTGCTTG CTTGGCACTG TCCTCTCATG CGTTGGGTCC 
     3601 ACTCAGTAGA TGCCTGTTGA ATTGGGTACG CGGCCAGCGG CGAGCGGTAT CAGCTCACTC 
     3661 AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC 
     3721 AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG 
     3781 GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC 
     3841 GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT 
     3901 TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT 
     3961 TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG 
     4021 CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT 
     4081 TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT 
     4141 TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG 
     4201 CTACACTAGA AGAACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA 
     4261 AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT 
     4321 TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC 
     4381 TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT 
     4441 ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA 
     4501 AAGTATATAT GAGTAACCTG AGGCTATGGC AGGGCCTGCC GCCCCGACGT TGGCTGCGAG 
     4561 CCCTGGGCCT TCACCCGAAC TTGGGGGGTG GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA 
     4621 TTGGCCCCAA TGGGGTCTCG GTGGGGTATC GACAGAGTGC CAGCCCTGGG ACCGAACCCC 
     4681 GCGTTTATGA ACAAACGACC CAACACCGTG CGTTTTATTC TGTCTTTTTA TTGCCGTCAT 
     4741 AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG 
     4801 GGCGAAGAAC TCCAGCATGA GATCCCCGCG CTGGAGGATC ATCCAGCCGG CGTCCCGGAA 
     4861 AACGATTCCG AAGCCCAACC TTTCATAGAA GGCGGCGGTG GAATCGAAAT CTCGTGATGG 
     4921 CAGGTTGGGC GTCGCTTGGT CGGTCATTTC GAACCCCAGA GTCCCGCTCA GAAGAACTCG 
     4981 TCAAGAAGGC GATAGAAGGC GATGCGCTGC GAATCGGGAG CGGCGATACC GTAAAGCACG 
     5041 AGGAAGCGGT CAGCCCATTC GCCGCCAAGC TCTTCAGCAA TATCACGGGT AGCCAACGCT 
     5101 ATGTCCTGAT AGCGATCCGC CACACCCAGC CGGCCACAGT CGATGAATCC AGAAAAGCGG 
     5161 CCATTTTCCA CCATGATATT CGGCAAGCAG GCATCGCCAT GGGTCACGAC GAGATCCTCG 
     5221 CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG AACAGTTCGG CTGGCGCGAG CCCCTGATGC 
     5281 TCTTCGTCCA GATCATCCTG ATCGACAAGA CCGGCTTCCA TCCGAGTACG TGCTCGCTCG 
     5341 ATGCGATGTT TCGCTTGGTG GTCGAATGGG CAGGTAGCCG GATCAAGCGT ATGCAGCCGC 
     5401 CGCATTGCAT CAGCCATGAT GGATACTTTC TCGGCAGGAG CAAGGTGAGA TGACAGGAGA 
     5461 TCCTGCCCCG GCACTTCGCC CAATAGCAGC CAGTCCCTTC CCGCTTCAGT GACAACGTCG 
     5521 AGCACAGCTG CGCAAGGAAC GCCCGTCGTG GCCAGCCACG ATAGCCGCGC TGCCTCGTCT 
     5581 TGCAGTTCAT TCAGGGCACC GGACAGGTCG GTCTTGACAA AAAGAACCGG GCGCCCCTGC 
     5641 GCTGACAGCC GGAACACGGC GGCATCAGAG CAGCCGATTG TCTGTTGTGC CCAGTCATAG 
     5701 CCGAATAGCC TCTCCACCCA AGCGGCCGGA GAACCTGCGT GCAATCCATC TTGTTCAATC 
     5761 ATGCGAAACG ATCCTCATCC TGTCTCTTGA TCGATCTTTG CAAAAGCCTA GGCCTCCAAA 
     5821 AAAGCCTCCT CACTACTTCT GGAATAGCTC AGAGGCCGAG GCGGCCTCGG CCTCTGCATA 
     5881 AATAAAAAAA ATTAGTCAGC CATGGGGCGG AGAATGGGCG GAACTGGGCG GAGTTAGGGG 
     5941 CGGGATGGGC GGAGTTAGGG GCGGGACTAT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     6001 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CTGGTTGCTG ACTAATTGAG 
     6061 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCCTAACTG 
     6121 ACACACATTC CACAGCTGGT TCTTTCCGCC TCAGGACTCT TCCTTTTTCA ATATTATTGA 
     6181 AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT 
     6241 AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGACGC GCCCTGTAGC 
     6301 GGCGCATTAA GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG TGACCGCTAC ACTTGCCAGC 
     6361 GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC TCGCCACGTT CGCCGGCTTT 
     6421 CCCCGTCAAG CTCTAAATCG GGGGCTCCCT TTAGGGTTCC GATTTAGTGC TTTACGGCAC 
     6481 CTCGACCCCA AAAAACTTGA TTAGGGTGAT GGTTCACGTA GTGGGCCATC GCCCTGATAG 
     6541 ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA ATAGTGGACT CTTGTTCCAA 
     6601 ACTGGAACAA CACTCAACCC TATCTCGGTC TATTCTTTTG ATTTATAAGG GATTTTGCCG 
     6661 ATTTCGGCCT ATTGGTTAAA AAATGAGCTG ATTTAACAAA AATTTAACGC GAATTTT
//